(JP8506731-W)      New DNA constructs (A) contain a sequence (I) encoding a Pyrococcus alpha-amylase (II), or its active and/or immunologically cross-reactive variants. (I) (1) comprises at least one of 5 partial sequences (117-3036p) reproduced in the specification or analogues able to hybridise with probes (P) derived from them, (2) corresponds to a genomic Pyrococcus sequence located within 5 kb of a sequence hybridising with P, or (3) is a 1380 bp sequence (reproduced) or analogues able to hybridise with probes (Pt) derived from it.    Also new are (1) DNA constructs (Ia) encoding related amylolytic enzyme, (2) vectors contg. the new constructs, (3) host cells contg. the new constructs or vectors, (4) (II) having a 435 amino acid sequence (reproduced), its variants or enzymes with at least 70% homology, also new amylolytic enzymes encoded by (Ia).    USE/ADVANTAGE -   (II) is used for liquefaction, saccharification and/or debranching of starch or for prodn. of syrups, cyclodextrin or oligosaccharides. Typically applications are in the wine and fruit industries, textile desizing, baking, detergents, biomass (waste) conversion for energy prodn. etc. DNA fragments (43 specified) present in (I) can be used to identify/isolate new amylolytic enzymes. (A) can be expressed in host cells without any modification to the ribosome binding site. (II) can be produced in good yield as recombinant protein with high purity i.e. without additional enzymatic activities. Because of its high heat stability, (II) is esp. useful in high temp. starch liquefaction.    (JP3201526-B2)      New DNA constructs (A) contain a sequence (I) encoding a Pyrococcus alpha-amylase (II), or its active and/or immunologically cross-reactive variants. (I) (1) comprises at least one of 5 partial sequences (117-3036p) reproduced in the specification or analogues able to hybridise with probes (P) derived from them, (2) corresponds to a genomic Pyrococcus sequence located within 5 kb of a sequence hybridising with P, or (3) is a 1380 bp sequence (reproduced) or analogues able to hybridise with probes (Pt) derived from it.    Also new are (1) DNA constructs (Ia) encoding related amylolytic enzyme, (2) vectors contg. the new constructs, (3) host cells contg. the new constructs or vectors, (4) (II) having a 435 amino acid sequence (reproduced), its variants or enzymes with at least 70% homology, also new amylolytic enzymes encoded by (Ia).    USE/ADVANTAGE -   (II) is used for liquefaction, saccharification and/or debranching of starch or for prodn. of syrups, cyclodextrin or oligosaccharides. Typically applications are in the wine and fruit industries, textile desizing, baking, detergents, biomass (waste) conversion for energy prodn. etc. DNA fragments (43 specified) present in (I) can be used to identify/isolate new amylolytic enzymes. (A) can be expressed in host cells without any modification to the ribosome binding site. (II) can be produced in good yield as recombinant protein with high purity i.e. without additional enzymatic activities. Because of its high heat stability, (II) is esp. useful in high temp. starch liquefaction.