NOVELTY - DNA fragment (I) of 5.8 kilo bases (kb) containing a toluene monooxygenase (Tom) gene and 1 BamH1, 2 EcoRI, 1 Hpa1, 1 Kpn1, 1NcoI, 1 NspV, 1 Sac, 2 SmaI, 3 SphI, 2 XhoI, and no ClaI, DraI, EcoRV, HindIII, NdeI, NheI, PvuII, ScaI, See8387I, StuI or XbaI restriction site, and comprising a restriction map given in the specification, is new.    USE - The nucleic acids and hosts are useful for the recombinant production of TOM. The hosts and TOM are useful for degrading a chlorinated aliphatic hydrocarbon compound (e.g. trichloroethylene (TCE) or dichloroethylene (DCE)), or an aromatic compound (e.g. 1 of toluene, benzene, phenol, and cresol), e.g. in environmental remediation.    ADVANTAGE - The TOM has a high efficiency in degrading aromatic and volatile organic chlorinated compounds.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for the following:    (1) a recombinant DNA comprising a vector encoding (I);    (2) a DNA encoding a TOM comprising 5 sequences encoding TomL (355 amino acids (aa); (II)), TomM (89 aa; (III)), TomN (516 aa; (IV)), TomO (118 aa; (V)), and TomP (352 aa; (VI)), given in the specification, aligned so that expressed TomL-TomP polypeptides can form an active protein;    (3) a DNA encoding an active variant of at least 1 of TomL-TomP;    (4) a DNA encoding a TomK polypeptide of 76 aa (VII), given in the specification, or its active variants;    (5) recombinant DNA's comprising a vectors encoding a DNA as in (2) and/or a DNA as in (4), linked to a promoter or promoters;    (6) a transformant obtained by introducing any of the vectors of (1) and (5) to a host microorganism;    (7) producing TOM comprising culturing the host of (6);    (8) cleaning a medium or environment polluted with, or degrading at least 1 chlorinated aliphatic hydrocarbon compound and/or an aromatic compound in a medium using a transformant as in (6);    (9) a polypeptide comprising 1 of aa's (II)-(VII), or a sequence of 118 aa (VIII), given in the specification; and    (10) a TOM comprising at least TomL-TomP, or its active variants.    DESCRIPTION OF DRAWING(S) - The diagram shows a restriction map of a 5.8 kilo bases (kb) DNA fragment (I) carrying a toluene monooxygenase (Tom) gene.