TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Process: An enzyme is prepared by cultivating a microorganism selected from the group of bacteria, fungi and/or hypomycetes on a nutrient substrate.Preferred Enzymes: The enzymes are alpha amylase and pectic lyase. Waste water produced after separating the oil is subjected to microbial fermentation using an inoculum of Saccharomyces ceravisiae to recover the solid residues of the polysaccharide. Then, residue is subjected to microbial fermentation using an inoculum of Candida utilis to recover a solid bio-mass and conditional water, is recycled to prepare nutrient substrate to produce the specified enzyme.Preferred Conditions: Enzymes are used at a concentration of 0.5-10mg/kg of solid mass to be treated at a temperature of 35+/-2degreesC and at pH between 5.5-7.8 based on the specific enzymes used.    TECHNOLOGY FOCUS - BIOLOGY - Preferred Microorganisms: Bacterial species used for producing alpha-amylase includes Bacillus cereus and/or Bacillus subtilis. Other species includes Aspergillus horizae, Cladosporium resinae, Cryptococcus luteoleus, Filobasidium capsoligenum, Lipomyces konoenkoaue, Saccharomycopsis capsularis, Saccharomycopsis fibuligera and/or Schwanniomyces occidentalis. Species that produce pectin lyase includes Bacillus polymixa, Colletrichum lindemuthianum, Aspergillus japonicus, Monilinia fructigena and/or penicillium expansum.An INDEPENDENT CLAIM is included for the recycling of biomasses and conditioned wastewater to prepare the nutrient substrate for the local production of at least one enzyme. EXAMPLE - Primary extraction of oil from soya seeds comprises:    (a) Preparation Of Enzyme - Bacillus cereus was cultured in a nutrient broth (starch absent) to produce alpha-emylase. After culturing, filtration of the broth was done to recover the enzyme.    (b) Enzyme Attack - The broth containing the enzyme was poured into a paste mixer to which soya was introduced. The mass was corrected to pH between 6-6.1 at a temperature of 35+/-2degreesC and agitated for 1-2 minutes.    (c) Pressing And Centrifugation - After the enzyme reaction, the resulting mass was pressed with presses to produced solid cake and liquid part. The liquid part was centrifuged to separate water, oil and lecithin from solid residues.    (d) Separation Of Lecithin and Oil - Oil was separated by centrifugation and send to refining.    (e) Metabolization Of Processed Waste Water - The water that remains after centrifugation had D-glycuronic acid and pentasaccharides (products of breakdown of inulin by alpha amylase enzyme). Culturing with Saccharomyces cerevisiae and Candida utilis produced the biomasses which were sterilized and used as protein for zeo-technical use or for preparation of culture broths.