TECHNOLOGY FOCUS - BIOLOGY - Preferred Process: Method (9) is applied to films on hard surfaces that are impermeable to microbes, particularly those made of metal, synthetic polymer, rubber, concrete, board, glass, wood, paper, textile, rock, marble, gypsum, ceramics, paint and enamel. The hard surface is preferably a member of a system holding, transporting or processing or is in contact with aqeous solutions, such systems are water supply systems, food processing systems, cooling systems or chemical, pharmaceutical or wood processing systems. The hard surface is a member of one of system units pipes, tanks, heat-exchangers, centrifuges, evaporators, mixers, spray towers, valves and reactors. The hard surface may alternatively be a member of a utensil such as endoscopes, prosthetic devices or medical implants. Especially the method is a Cleaning In Place process. Alternatively, the surfaces are of biological origin (e.g. mucosa, skin, teeth, hair, nails and mucous membranes), and the method is then particularly used for removal of dental plaque. In process (10), (I) may be formulated with other hydrolases, e.g. (hemi)cellulase, xylanase, amylase, lipase, protease and pectinase, and/or an antimicrobial agent such as laccase or peroxidase.Preferred Composition: Composition (8) is particularly for oral care and is presented as powder, paste, gel, liquid, ointment or tablet. Other enzymes (e.g. dextranase, mutanse, (per)oxidase etc.) and conventional abrasives, humectants etc. may also be included. Alternatively, (8) is a detergent additive or detergent composition, optionally containing surfactants and other standard detergent enzymes.    TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Enzymes: Proteins (A), (B) and (C) are from Paenibacillus amylolyticus, P. pabuli and P. macerans, respectively. (I) is, or comprises, (A), (B) or (C), or their fragments, and is particularly encoded by the inserts, in plasmid pSJ1678, present in the Escherichia coli strains DSM 12406, 13028 and 13029. (I) has at least 20% of the FH activity of (A), (B) and (C).Preferred Nucleic Acid: (II) may include at least one mutation in the region encoding the mature polypeptide.Preparation: Typically, genomic DNA from P. amylolyticus DSM 12458 was isolated and amplified with the primers 5'-CATTCTGCAGCAGCGGCGGCTATGGCTGTACTTGC 5'-CGCGGATCCAGATCTTACATGGAGTCCAAGCTTTC The amplicon was cloned as two fragments and the recombinant plasmid used to transform Bacillus subtilis SHa 273, for expression of recombinant FH. More generally, once isolated the DNA can be expressed in any standard vector/host system. ACTIVITY - Anticarie; Neuroprotective.    MECHANISM OF ACTION - None given.    ADMINISTRATION - The protein or its mutant is preferably administered as a powder, paste, gel, liquid, ointment or tablet e.g. as toothpaste, mouth wash or chewing gum (claimed).    EXAMPLE - A biofilm was established (from microbes in tap water) on steel disks, then these treated for 2 hr at pH 6.5 and 50degreesC with a solution containing recombinant 2,6-beta-D-fructan hydrolase. Significant biofilm removal was observed and there was a very large synergistic effect when the enzyme was used together with a BioCip membrane (containing protease and several different carbohydrolase enzymes).