TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Enzymes: The enzymes produced by the microorganism can be manganese peroxidase, lignin peroxidase, laccase, benzene monooxygenase or benzene dioxygenase.Preferred Microorganism: The microorganism producing the enzyme is selected from Schizophillum, Pleurotus, Trametes, Lentinus, Rhizoctonia, Funalia, Pycnoporus, Merulius, Mycliophtora, Coprinus, Flammulina, Ganoderma, Daedaleopsis, Favolus, Lyophyllum, Asperugillus and Auricularia.Preferred Process: The hardly degradable harmful material can be dioxins, halogenated biphenyls, halogenated hydrocarbons, bisphenols, alkylphenols, halogenated phenols and phthalates. The treatment on miscellaneous bacteria is by heating and/or chemical treatment. Heating is particularly at not less than 60degreesC, especially in combination of the use of oxygen or water and an exothermic material to generate heat by reaction. Typically, the contaminated soil is mixed and stirred with oxygen or water and not less than 5 wt.% the exothermic material, with respect to the dry soil, followed by adding water for exothermic reaction to keep the soil at not less than 60degreesC, and storing until the temperature is lowered to not more than 40degreesC for charging in the microorganism with capability of degrading the specified material. The exothermic material can be calcium oxide, iron powder, graphite, sulfur, iron(II) hydroxide, magnesium, molybdenum, nickel, tris(acetylacetonate)aluminum, bis(acetylacetonate)copper, tris(acetylacetonate)iron(III), bis(acetylacetonate)magnesium, dibenzenemolybdenum, molybdenum hexacarbonyl, diethylsilanediol, or/and diethylzinc. During the process, pH of the soil is adjusted to 4-10. The chemical treatment is by using the agents selected from ethyl alcohol, ethylene oxide, reversible soap, limonene, veratryl alcohol, diethyl carbonate, hydrogen peroxide, hypochloric acid, hydrochloric acid and chlorpicrin. After charging nutrients to the contaminated soil, ash or water, a microorganism is added for contact. The miscellaneous bacteria are removed then the required microorganism or its enzyme is added for contact; or the residual inorganic chlorides are removed or decomposed into chloride ion before adding the microorganism. The hardly degradable harmful material is decomposed in water at pH 3-11. When promoting degradation of the hardly degradable harmful material by the enzyme produced from the microorganism, this is performed in the presence of 1 or more of surfactants, oleic acid, olive oil, linseed oil and limonene. EXAMPLE - Dioxin-contaminated soil in water was heated at 65degreesC for 1 hour and then cultured with Schizophillum commune IFO 6505 and added nutrients, at 25degreesC for 30 days (TEQ = 2560 pg/g based on inputted 2,3,7,8-tetrachlorodibenzo-p-dioxin).