TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation: Producing (I) involves incubating a host cell genetically engineered to express the polynucleotide, which encodes (I). The preparation further involves isolating Gux1 polypeptide from incubated host cells. The host cell is a plant or bacterial cell. The host cell is genetically engineered to express the selectable marker. The host cell further comprises polynucleotide molecule encoding one or more polypeptide molecules chosen from glycoside hydrolase family of proteins. The glycoside hydrolase is a thermostable glycoside hydrolase (claimed).Preferred Polypeptide: (I) has at least 90% sequence identity with the amino acids sequence of (1-5). Preferred Composition: In (II), Gux1 peptide is further defined as comprising a linker and signal peptide. The GH48 catalytic domain of Gux1 peptide is further defined as having a length of 637-643 amino acids. The CBD III of the Gux1 peptide further defined as having a length of 150-156 amino acids. The CBD type (II) of Gux1 peptide is further defined as having a length of 95-105 amino acids. The GH48 catalytic domain is further defined as the sequence of (2). The CBD type (III) is further defined as the sequence of (1). The CBD type (II) is further defined as the sequence of (4). (II) is further defined as comprising a sequence of (1), (2), and (4). The Gux1 is further defined as comprising a nucleic acid sequence having at least 90%, 80% or 70% sequence identity to the nucleic acid sequence encoding an amino acid sequence of (2). The Gux1 is further defined as comprising a nucleic acid sequence having at least 90% sequence identity to nucleic acid sequence encoding the amino acid sequence of (1), (3), (4), or (5). The Gux1 is further defined as comprising a nucleic acid sequence encoding a heterologous protein in frame with the Gux1 peptide. The heterologous protein in frame with the Gux1 peptide is further defined as a peptide tag. The peptide tag is 6-His, thioredoxin, hemagglutinin, glutathione-S-transferase (GST) or OmpA signal sequence tag. The heterologous protein is a substrate targeting moiety. The nucleotide sequence encoding the Gux1 is operably linked to a transcriptional or translational regulatory sequence comprising a transcriptional promoter or enhancer.Preferred Mixture: The industrial mixture is further defined as comprising a detergent.Preferred Fusion Protein: The heterologous peptide is substrate targeting moiety or a peptide tag. The peptide tag is 6-his, thioredoxin, hemagglutinin, GST, or OmpA signal sequence tag. The heterologous peptide is an agent that promotes polypeptide oligomerization. The agent is a leucine zipper.Preferred Host Cell: In (IV), the host cell is a plant cell, fungi or a bacterial cell. Preferably, the host cell is a bacterial cell.Preferred Antibody: The antibody is a polyclonal or monoclonal antibody.Preferred Method: In (M1), an increase in carbohydrate degradation activity in the presence of the agent of interest demonstrates stimulation of Gux1 activity and where a decrease in carbohydrate degradation activity demonstrates inhibition of Gux1 activity. The carbohydrate is cellulose. The agent of interest is an antibody. EXAMPLE - Molecular cloning of Gux1 was done as follows. Genomic DNA was isolated from Acidothermus cellulolyticus and was purified by banding on cesium chloride gradients. Genomic DNA was partially digested with Sau 3A and separated on agarose gels. The DNA fragments in the range of 9-20 kbp were isolated from the gels. The purified Sau 3A digested genomic DNA was ligated into the Bam H1 acceptor site of purified EMBL3 lambda phage arms. Phage DNA was plated with Escherichia coli LE392 in top agar, which contained the soluble cellulose analog, carboxymethylcellulose (CMC). The plates were incubated overnight to allow transfection, bacterial growth, and plaque formation. Plates were stained with Congo Red followed by destaining with 1 M NaCl. The lambda plaques harboring endoglucanase clones showed up as unstained plaques on a red back ground. The lambda clones which is screened positive on CMC Congo Red plates were purified by successive rounds of picking, plating and screening. Individual phage isolates were named SL-1, SL-2, SL-3, and SL-4. Subsequent subcloning efforts employed the SL-3 clone which contained an approximately 14.2 kb fragment of A.cellulolyticus genomic DNA. The template DNA was constructed using a 9 kb Bam H1 fragment obtained from the 14.2 kb lambda clone SL-3 prepared from A.cellulolyticus genomic DNA. The 9 kb Bam H1 fragment from SL-3 was subcloned into pDR540 to generate a plasmid NREL501. NREL501 was sequenced by primer walking. Sequencing data from primer walking and subclones were assembled together to verify that all SL-3 regions had been sequenced from both strands. An open reading frame (ORF) was found in 9 kb Bam H1 fragment, C-terminal of E1, termed Gux1. An ORF of 3366 base pairs and amino acid sequence were deduced. The predicted amino acid sequence was determined to have significant homology to known cellulases. Sequence alignments and comparisons of the amino acid sequences of A.cellulolyticus Gux1 catalytic domain (amino acids 231-870), Cellulomonas fimi and Thermobifida fusca polypeptides were prepared using the ClustalW program. The determination of amino acid sequence alignment of the GH48 domains indicated that the amino acid sequence Gux1 catalytic domain was homologous to the amino acid sequences of known GH48 family catalytic domains of C.fimi.