TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Component: The CSC is a bacterial pheromone, eukaryotic hormone, or diffusible communication molecule. The bacterial pheromones are N-acyl homoserine lactones, pheromone peptides, N-acylated C-amidated D-amino acid hexapeptides, D-amino acid peptides comprising D-isoleucine and/or D-tyrosine, cyclic dipeptides, hydrophobic tryamines, lipopeptide biosurfactants, fatty acid derivatives, antimicrobial peptides, or furanones. The eukaryotic hormones are auxins, cytokinins, cytokines, ethylene gas, gibberlines, or abscisic acid. The CSC can be Rhodomine 123 and/or 3-hydroxypalmitic acid methyl ester, N-(3-oxohexanoyl)-L-homoserine lactone, 3-oxydodecanoyl homoserine lactone, N-butanoyl-L-homoserine lactone, 6-benzylamino-purine, extracts of Delisea pulchra, kinetin, indole-acetic acid, Rhodomine 123, Zeatin, 6-(gammagamma-dimethylallylamino)purine, 6-benylamino-purine, butyryl homoserine lactone, alkyl furanones, N-acyl homoserine lactone, peptides pheromones and/or histidine protein kinase pheromones. The regulated activity is modulated by gene expression in a microbial cell or is modulated by communication signals between microbial cells or populations of microbial cells. It can be cell to cell communication, quorum sensing, swarming, bacterial motility, symbiotic associations with multicellular organisms, cell metabolic rates, production of metabolic products, cell division and conjunction, cell resuscitation, formation of biofilm communities, entry into a stationary or dormant phase, discrete and diverse metabolic processes in concert with cell density, antibiotic production, or bioluminescence. The microbial population is Gram positive bacteria, Gram negative bacteria, cyanobacteria, autotrophic bacteria, heterotrophic bacteria, or nitrogen-fixing bacteria. It can be aerobic bacteria, facultative anaerobic bacterial, anaerobic bacteria, sulfur reducing bacteria, sulfate reducing bacteria, ammonia producing bacteria, nitrite producing bacteria, or methane producing bacteria. The activity of a microbial population is up regulated, initiated, or sustained by the addition of CSC. The CSC is added as a bolus, either continuously or at intervals. the CSC is added to the sewage substrate in a sewerage catchment network or a sewage treatment plant EXAMPLE - A sub-sample sewage (800 ml) was mixed with cell signaling chemical (CSC). Each identical time or identical sub-sample. Each sample was decanted and subjected to plate counts. If testing aerobic antimicrobial response, the samples were aerated. After 24 hours the sample from each replication was subjected to NATA certified procedures for standard plate counts.