TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: In (M1), the support is made to hold one type of (I). The concentration of bacteria held by support ranges from 107 piece- 108 piece/g of support, which is a wood chip. The moisture content of the support is 40-60%. The moisture content of support and organic waste is adjusted to 35% or more and 45% or less after application of the support, by supplying air to the treatment tank. The temperature of the treatment tank is maintained at 30 degreesC or more. The support and organic waste are stirred in the treatment tank. EXAMPLE - The contents of an organic refuse-disposer were transferred to a treatment tank. The temperature of the tank was maintained at 30 degreesC and the contents were mixed using a stirrer. The moisture content was maintained below 75% and 26.1 kg of wood chip was added to the tank to serve as support. Organic refuse consisting of 450 g vegetables, 300 g fruit, 40 g fish, 30 g meat and 280 g of cooked rice was further added into the treatment tank and stirred continuously with a moderate supply of air. Sixteen kinds of bouillon culture medium varying in salt concentration and pH were prepared. From the treatment tank 10 g of organic refuse was collected and diluted using dilution liquid. The liquids were inoculated into the prepared culture plates and cultivated. A total of 306 strains were collected from seven kinds of agar media. The isolated strains were further categorized based on the electrophoresis profile amplified by random amplified polymorphic DNA (RAPD) and 232 strains were obtained. The ability of the obtained bacteria to survive in the organic refuse as microbial flora was analyzed. These bacteria were compared with the bacteria stored in the database and the similarity ratio was determined. Seventeen kinds of bacteria having 0.07 or more ratio value with the bacteria stored in data base were selected. Based on the ability of strains to exist in the microbial flora, seven kinds of bacteria namely SA1001, SA1014, SA1031, SA1036, SA3007, SA4004 and SA6002 were identified and specified as spawn.