TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Methods: The algae is green algae and is an isolated strain with down regulated expression of sulfate permease with 50 % or less expression of sulfate permease relative to normal wild-type algae. The algae can be genetically modified by insertion of an antisense sequence to CrcpSulP, insertion of sense strand of CrcpSulP, ablation of CrcpSulP, or targeted gene deletion of CrcpSulP. The antisense sequence hybridizes to a portion of nucleic acid comprising 3840 base pairs.Producing hydrogen comprises culturing a combination of sulP1 strain of Chlamydomonas reinhardtii and Rhodobacter sphaeroide with Clostridium species.Generating hydrogen gas preferably comprises subjecting a biomass comprising an algae to sunlight in a sulfur-containing media comprising carbon dioxide and inorganic nutrients for a period of time and under conditions to cause the algae to undergo oxygenic photosynthesis and to generate hydrogen gas; and subjecting an anaerobic photosynthetic bacterium in the media to sunlight for a period of time to generate hydrogen from a nitrogenase/hydrogenase enzymatic system in the media. The method further comprises inducing fermentation of the biomass the media of Chlamydomonas/ Rhodobacter via Clostridium species.Generating hydrogen gas comprises providing in an aqueous media a genetically modified strain of Chlamydomonas reinhardtii, providing a strain of Rhodobacter sphaeroide and exposing to sunlight for generation of hydrogen and biomass, subjecting an anaerobic photosynthetic bacterium to sunlight to generate hydrogen from a nitrogenase/hydrogenase enzymatic system, providing a strain of Clostridium in the medium and inducing fermentation of the biomass in the media via Clostridium. The genetically modified algae is modified to decrease activity of sulfate permease by a technique selected from insertion of an antisense strand of a sulfate permease gene, insertion of a sense strand of a sulfate permease gene, ablation of the sulfate permease gene, or targeted gene deletion of the sulfate permease gene.Preferred Compositions: The algae containing-composition further comprises Clostridium species having the lineage bacteria, Firmicutes, Clostridia, Clostridiales, or Clostridiaceae. Preferred Components: The algae can be green algae. It is artificially engineered to reduce sulfate permease expression relative to a wild-type algae. It can be unicellular, photosynthetic, or anoxygenic algae. It can be Rhodobacter sphaeroide or genetically modified Chlamydomonas reinhardtii. The Rhodobacter sphaeroide is anoxygenic photosynthesis bacterium having a lineage of Proteobacteria; Alphaproteobacteria, or Rhodobacterales; Rhodobacteraceae.Preferred Nucleic Acid: The isolated antisense oligonucleotide comprises a sequence complementary to codons 118-412 of N1 comprising 3840 base pairs.