WO2003033533-A  "M-110S Microfluidizer" MICROFLUIDICS CORPORATION, [Online] 1998, XP002202508 Retrieved from the Internet: &lt;URL:http://www.microfluidicscorp.com/mi_e _110s.htm&gt; [retrieved on 2002-06-18];  DE LUCA P M ET AL: "Evaluation of the stability and immunogenicity of autoclaved and nonautoclaved preparations of a vaccine against American tegumentary leishmaniasis" VACCINE, BUTTERWORTH SCIENTIFIC. GUILDFORD, GB, vol. 17, no. 9-10, 5 March 1999 (1999-03-05), pages 1179-1185, XP004158241 ISSN: 0264-410X;  LEWIS R.: "New Disrupters help cell biologists retrieve products" THE SCIENTIST, [Online] vol. 24, no. 18, 13 December 1993 (1993-12-13), page 18 XP002202507 Retrieved from the Internet: &lt;URL:http://www.the-scientist.com/yr1993/d ec/tools_931213.html&gt; [retrieved on 2002-06-18];  REED S G ET AL: "SELECTION OF A SKIN TEST ANTIGEN FOR AMERICAN VISCERAL LEISHMANIASIS" AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, vol. 35, no. 1, 1986, pages 79-85, XP002202015 ISSN: 0002-9637 cited in the application;  SCOTT P ET AL: "VACCINATION AGAINST CUTANEOUS LEISHMANIASIS IN A MURINE MODEL I. INDUCTION OF PROTECTIVE IMMUNITY WITH A SOLUBLE EXTRACT OF PROMASTIGOTES" JOURNAL OF IMMUNOLOGY, vol. 139, no. 1, 1987, pages 221-227, XP002202016 ISSN: 0022-1767;  STITELER J M ET AL: "ODS Leishmania skin test, MFL-LSTA(R2): Stability of the cGMP product in the guinea pig animal model." AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, vol. 62, no. 3 Supplement, March 2000 (2000-03), page 310 XP008004176 49th Annual Meeting of the American Society of Tropical Medicine and Hygiene;Houston, Texas, USA; October 29-November 02, 2000 ISSN: 0002-9637WO2003033533-A1  &quot;M-110S Microfluidizer&quot; MICROFLUIDICS CORPORATION, [Online] 1998, XP002202508 Retrieved from the Internet: URL:http://www.microfluidicscorp.com/mi_e _110s.htm [retrieved on 2002-06-18];  LEWIS R.: &quot;New Disrupters help cell biologists retrieve products&quot; THE SCIENTIST, [Online] vol. 24, no. 18, 13 December 1993 (1993-12-13), page 18 XP002202507 Retrieved from the Internet: URL:http://www.the-scientist.com/yr1993/d ec/tools_931213.html [retrieved on 2002-06-18];  DE LUCA P M ET AL: &quot;Evaluation of the stability and immunogenicity of autoclaved and nonautoclaved preparations of a vaccine against American tegumentary leishmaniasis&quot; VACCINE, BUTTERWORTH SCIENTIFIC. GUILDFORD, GB, vol. 17, no. 9-10, 5 March 1999 (1999-03-05), pages 1179-1185, XP004158241 ISSN: 0264-410X;  SCOTT P ET AL: &quot;VACCINATION AGAINST CUTANEOUS LEISHMANIASIS IN A MURINE MODEL I. INDUCTION OF PROTECTIVE IMMUNITY WITH A SOLUBLE EXTRACT OF PROMASTIGOTES&quot; JOURNAL OF IMMUNOLOGY, vol. 139, no. 1, 1987, pages 221-227, XP002202016 ISSN: 0022-1767;  STITELER J M ET AL: &quot;ODS Leishmania skin test, MFL-LSTA(R2): Stability of the cGMP product in the guinea pig animal model.&quot; AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, vol. 62, no. 3 Supplement, March 2000 (2000-03), page 310 XP008004176 49th Annual Meeting of the American Society of Tropical Medicine and Hygiene;Houston, Texas, USA; October 29-November 02, 2000 ISSN: 0002-9637;  REED S G ET AL: &quot;SELECTION OF A SKIN TEST ANTIGEN FOR AMERICAN VISCERAL LEISHMANIASIS&quot; AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, vol. 35, no. 1, 1986, pages 79-85, XP002202015 ISSN: 0002-9637 cited in the applicationEP1436325-A1  See references of WO 03033533A1