TECHNOLOGY FOCUS - BIOLOGY - Preferred Method: The activity of the cultivated microorganisms is maintained effectively due to the presence of ocean deep water. The culture medium obtained is subjected to high pressure wet-sterilization process and placed in a high pressure container. The seed yeast of microorganisms is inoculated in the culture medium and stationary culture is performed in the water bath.Preferred Microorganisms: The microorganisms which are cultivated in the culture medium are thermophilus or normal temperature microorganisms such as Thermophilic actinomycetes (MH-1), Thermophilus (C-1) microbes of Bacillus brevis, and Bacillus stearothermophilus (CH-4) and microorganisms selected from deposition microorganisms of Thermophilus inoculum (PTA-1773, MIROKU). The culture medium components are glucose, polypeptone, yeast extract, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, carbon source such as glycerol, cane sugar and starch, nitrogen source such as yellow tail fish and cotton seed powder and metallic salt of manganese and iron. EXAMPLE - Microbes of Thermophilus inoculum were obtained from scum of shrimp, crab and small fish. 4 types of culture medium were obtained by dissolving YPD culture medium components in deionized water (I), 3.5 % sodium chloride solution (II), sea surface layer water (III) and in ocean deep water (IV). The YPD culture medium contained (in g) glucose (10), polypeptone (8), yeast extract (7), potassium dihydrogen phosphate (1.0) and magnesium sulfate heptahydrate (0.5). The carbon and nitrogen source were added to the culture medium and autoclaved by high pressure wet sterilization at 121 degreesC for 15 minutes. The microbial cells were inoculated in the culture medium and cultured at 590 degreesC. The turbidity and colony forming unit of the culture solution was measured on 7th day. The result showed that the number of microbes in the culture mediums (III and IV) were high when compared to other culture mediums (very few).