NOVELTY - A strain (I) of Bacillus alkalophilus and strain of Bacillus sp. having the following characterisitics: grows at a pH range from 8.0-11.0 and does not grow at neutral pH, and produces acid in the presence of carbohydrates, is new.    USE - (M1) is useful for neutralizing alkaline waste-waters, which involves reconstituting lyophilized bacterial powder by adding distilled water, adding reconstituted bacterial strain solution in alkaline medium and alkaline waster-water containing phenol red indicator, observing acid production by the change in color of phenol red in modified nutrient broth medium and in the alkaline waste-waters from red to orange, orange to yellow, and measuring the decrease in pH of the medium as well as of alkaline waste-waters for a period of two days (claimed). Acclimatized and lyophilized mixed bacterial powder of Bacillus alkalophilus (CBTCC/Micro/8 MTCC 5092) and Bacillus sp. (CBTCC/Micro/8 MTCC 5093) were reconstituted in 50 ml sterile triple distilled water overnight. The mixed reconstituted bacterial culture was used for the neutralization of alkaline waste-waters of textile industry. Three different sets of alkaline waste-waters were prepared to observe the acid production. In the first set, 10% of the mixed reconstituted bacterial suspension was added in the waste-waters. In the subsequent two sets, 10% of the mixed reconstituted bacterial suspension was added in waste-waters containing 1% glucose and 1% sucrose, respectively. All the three sets were incubated at 37degreesC for a period of 7 days in an incubator shaker at 75 rpm acid production by microbial composition was observed in all the three sets after 2, 4 and 7 days of incubation period. A significant change was observed in the original pH of the waste-waters of textile industry after regular time intervals. Decrease in original pH of waste-waters was less in the absence of carbohydrates as compared to that with carbohydrates. Among carbohydrates, original pH decreased markedly in the presence of 1% glucose in comparison to 1% sucrose.    ADVANTAGE - (I)-(III) enables cost-effective and safe neutralization of alkaline waste-waters.    DETAILED DESCRIPTION - A strain (I) of Bacillus alkalophilus having the following characterisitics: grows at a pH range from 8.0-11.0 and does not grow at neutral pH, produces acid in the presence of carbohydrates, grows on blood sugar, does not produce beta-galactosidase and xylanase, and produces alkaline protease, or a strain (II) of Bacillus sp. having the following characterisitics: grows at a pH range from 8.0-11.0 and does not grow at neutral pH, produces acid in the presence of carbohydrates, hydrolyses casein, gelatin and starch but unable to hydrolyze urea, reduces nitrate, and produces alkaline beta-amylase.    An INDEPENDENT CLAIM is also included for a microbial composition (II), comprising a synergistic mixture of equal proportions of (I) and (II), and capable of neutralizing alkaline waste-waters.