NOVELTY - Producing whole cell biomass and biosurfactants from waste oil through bioremediation comprises providing an oleophilic suspension comprising a physiologically active, species/strains of bacteria that are oil-consuming, in a fatty acid.    USE - The method is useful for producing whole cell biomass and biosurfactants from waste oil through bioremediation (claimed).    DETAILED DESCRIPTION - The method comprises:    (A) isolating a source of waste oil in a container;    (B) providing an oleophilic suspension comprising physiologically active, species/strains of bacteria that are oil-consuming, in a fatty substance, comprising an oleophilic nutrient as a source of nitrogen and phosphorus for the bacteria, and admixing it with the source of waste oil and an excess of water;    (C) agitating the mixture of waste oil, oleophilic suspension and water for a fixed period of time, to produce biomass, carbon dioxide, and water by the consumption of oil by the bacteria;    (D) removing the residual oil by solvent extraction;    (E) centrifuging the remaining mixture at a low to medium speed of less than about 2000 rpm, to separate the whole cells, from the cell free, oil-free, water-based liquor;    (F) filtering the liquor through 0.22 micron filter paper;    (G) collection of cell free nutrient rich filtrate;    (H) precipitation of a bio-surfactant, extracellular polymeric substance from filtrate produced in step F by adding a solvent selected from water mixable acetone, alcohol and inorganic salts to the filtrate, storing the mixture for at least about 10 hours at 4degreesC for precipitation; and    (I) centrifuging at a speed less than about 20000 rpm, to separate the precipitate.    INDEPENDENT CLAIMS are included for the following:    (1) a nutritional supplement comprising protein isolated from the biomass produced by the method cited above;    (2) a nutritional supplement isolated from the cell free extract produced by the method;    (3) a method of producing vitamins, antibiotics and tailor made bio-surfactant, where the bacteria are pure strains of bacteria or yeasts, or genetically modified bacterial or yeasts species;    (4) a continuous batch method, comprising the any of the methods cited, where the solvent and oil extracted in the fourth step are separated, the solvent recycled, and the residual oil recycled to the container;    (5) a biosurfactant obtained by solvent precipitation of the cell free extract produced by the novel method;    (6) a nutritional supplement comprising the extra cellular polymeric substance produced as above;    (7) an economical method of producing whole cell biomass comprising about 24% by weight carbohydrate and about 34% by weight protein, from waste oil;    (8) a method for eliminating waste oil.