TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: In (M1), the concentration of the growth medium in the receiving selective enrichment buffer has a growth medium concentration which is equal to or considerable higher than recommended by the manufacturer of growth medium or approved procedure for the pathogen target-bacterium after addition of the selective growth medium. The pre-enrichment medium is pre-warmed to a temperature preferably above 30degreesC, more preferably above 32degreesC and most preferably above 34degreesC. The pre-enrichment medium is prewarmed to 37degreesC+/-3degreesC, preferably to 37degreesC. The transfer of a transfer volume form the pre-enrichment to the selective enrichment preferably is carried out after less than 12 hours, more preferably after 6+/-4 hours, more preferably after 6+/-2 hours, most preferably after 6 hours. The transfer of a transfer volume from pre-enrichment to the selective enrichment preferably is carried out after less than 2 hours. The transfer volume from the pre-enrichment to the selective enrichment is preferably of at least 2 mL, 5 mL, 10 mL, 20 mL, 50 mL, respectively preferably at least 2/200, 5/200, 10/200, 20/200, and 50/200 of the selective growth medium. The SELECTA BIOLINE substrate is applied for the selective enrichment. A pre-enrichment buffer and at least 2 selective growth media are applied. The initial enrichment medium is added with growth enhancers, resuscitation enhancers or selective or partly selective compounds such as selenite, tetrathionat, Novobiocin, antibiotics, Brilliant Green or Malachite green. The selective enrichment is shortened to 1, 2, 4, 6, 8 or 17 hours. The second buffer applied for the enrichment of target-organisms contains tetrathionat, Brilliant Green or Malachite Green. A method of analysis is applied for the detection step including but not restricted to: affinity binding technique such as enzyme immunoassays (enzyme linked immunosorbent assay (ELISA)) based on antigen-antibody reactions, antigen-antibody reactions which involves fluorescence, luminescence, evanescent waves, plasmon resonance, latex agglutination, electrochemical immune detection, technique with immunomagnetic capturing, technique with lateral flow, DNA hybridization based on specific sequences of DNA molecule, RNA-DNA, RNA-RNA, PCR based on multiplication by specific DNA primers, conductance measuring methods based on change in the electric resistance in special growth media, microscoping, techniques with micro arrays, charge coupled device (CCD) camera technique, enzyme immuno technique based on chromogene, fluorescence, luminescence, radioactive signal generating responds, semisolid agars and solid agars or combination with further enrichment procedures. The enzyme linked immunosorbent assay (ELISA) test kit of Bioline ApS is applied for the detection step. The transfer of the transfer volume from the pre-enrichment to the selective enrichment is carried out automatically or semi-automatically.