WO2006093998-A2  KING P.W. ET AL.: 'Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System' JOURNAL OF BACTERIOLOGY vol. 188, no. 6, March 2006, pages 2163 - 2172, XP009096433;  See also references of EP 1853716A2US8835153-B2  Posewitz et al. (JBC, 2004, vol. 279: 25711-25720).;  Stoker et al. Journal of Bacteriology, 1989, vol. 171, No. 8, pp. 4448-4456.;  Chaudhuri et al. Journal of General Microbiology, 1987, vol. 133, pp. 3289-3298.;  Posewitz et al., "Discovery of Two Novel Radical S-Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase," J. Biol. Chem. vol. 279, No. 24, 25711-25720 (2004).;  International Search Report dated Aug. 16, 2006, for International Application No. PCT/US2006/007153.;  International Preliminary Report on Patentability dated Sep. 11, 2007, for International Application No. PCT/US2006/007153.;  Supplementary European Search Report dated Jun. 17, 2008, for European Application No. EP 06 73 6467.;  Forestier, et al, "Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under Anaerobic Conditions", Eur. J. Biochem., vol. 270, 2750-2758 (2003).;  Nixon, et al, "Iron-Dependent Hydrogenases of Entamoeba histolytica and Giardia lamblia:. Activity of the Recombinant Entamoebic Enzyme and Evidence for Lateral Gene Transfer", Biol. Bull. vol. 204: 1-9 (Feb. 2003).;  Happe, et al, "Differential Regulation of the Fe-Hydrogenase during Anaerobic Adaptation in the Green Alga Chlamydomonas reinhardtii", Eur. J. Biochem., vol. 269, 1022-1032 (2002).;  Posewitz, et al, "Discovery of two Novel Radical SAM Proteins Required for the Assembly of an Active [Fe]-hydrogenase", JBC Papers in Press, Manuscript (Apr. 13, 2004).;  Ghirardi, et al, "Biological Systems for Hydrogen Photoproduction", DOE Hydrogen Program, FY 2004 Progress Report.;  Davies J.P. et al, "Sac3, an Snf1-like Serine/Threonine . . . to Sulfur Limitation", Plant Cell, vol. 11, 1179-1190 (1999).;  V. Lumbreras et al, "Efficient Foreign Gene . . . by and Endogenous Intron", Plant J., 441-448 (1998).;  H. J. Sofia et al, "Radical SAM . . . Visualization Methods", Nucleic Acids Res., 1097-1106 (2001).;  P. M. Vignais et al, "Classification and Phylogeny of Hydrogenases", FEMS Microbiol. Rev., vol. 25, 455-501 (2001).;  T. Happe et al, "Isolation, Characterization and N-terminal Amino Acid Sequence of Hydrogenase from the Green Alga Chlamydomonas reinhardtii", Eur. J. Biochem., vol. 214, 475-481 (1993).;  N. K. Menon et al, "Mutational Analysis and Characterization of the Escherichia coli hya Operon, Which Encodes [NiFe} Hydrogenase 1", J. Bacteriol., vol. 173, 4851-4861 (1991).;  A. Jacobi et al, "The hyp Operon Gene Products are Required for the Maturation of Catalytically Active Hydrogenase Isoenzymes in Escherichia coli", Arch. Microbiol, vol. 158, 444-451(1992).;  R. M. Allen "Incorporation of Iron and Sulfur from NifB Cofactor into the Iron-Molybdenum Cofactor of Dinitrogenase", J. Biol. Chem., vol. 270, 26890-26896 (1995).;  G. Pan et al, "Characterization of a [2Fe-2S] Protein Encoded in the Iron-Hydrogenase Operon of Thermotoga maritima", J. Biol. Inorg. Chem., vol. 8, 469-474 (2003).;  L. Casalot et al, "Maturation of the [NiFe] Hydrogenases", Trends Microbiol., vol. 9, 228-237 (2001).;  G. Voordouw et al, "Purification and Characterization of Desulfovibrio vulgaris (Hildenborough) Hydrogenase Expressed in Escherichia coli", Eur. J. Biochem, vol. 162, 31-36 (1987).;  Y. Asada et al, "Heterologous Expression of Clostridial Hydrogenase in the Cyanobacterium Synechococcus PCC7942", Biochem. Biophys. Acta., vol. 1490, 269-278 (2000).;  L. Girbal et al, "Homologous and Heterologous . . . with High Specific Activities", Appl. Env. Microbiol., vol. 71, 2777-2781 (2005).;  K. L. Kindle "High-Frequency Nuclear Transformation of Chlamydomonas reinhardtii" Proc. Natl. Acad. Sci., USA, vol. 87, 1228-1232 (1990).;  Ghirardi et al., "Microalgae: a Green Source of Renewable H2", Trends in Biotechnology, Elsevier, vol. 18, No. 12, 2000, pp. 506-511.;  King, et al, "Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System", Journal of Bacteriology, vol. 188, No. 6, 2163-2172 (2006).