TECHNOLOGY FOCUS - BIOTECHNOLOGY - Isolation (disclosed): (S1) is isolated from the west coast mudflat of Korea.Preferred Protease: (I) has an optimum temperature of 55-65degreesC; optimum pH of 10.0-11.0; is stable for more than 72 hours in the presence of sodium dodecyl sulfate (SDS) surfactants, oxidants such as hydrogen peroxide and sodium perborate, organic solvents and heavy metal ions; retains enzyme activity more than 75% under the concentration of 0.1-20% NaCl; and has molecular weight of 28 kDa as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). (I) has a fully defined 381 or 275 amino acid (SEQ ID No. 4 or 5) sequence, as given in the specification. (I) is encoded by a fully defined 2280 base pair (SEQ ID No. 3) sequence (II), as given in the specification.Preferred Vector: The vector (V1) is pT7-BCAP. The vector (V2) is pHPS-BCAP.Preferred Transformant: (IIIa) is Escherichia coli JM109/pHPS-BCAP deposited under Accession No: KCTC 10772BP. Preferred Method: In the purification method, the synthetic absorbant of the eluting step is a diaion HP synthetic absorbant. The chromatography is hydrophobic and ion exchange chromatography. The hydrophobic chromatography is performed on phenyl-Sepharose column and the ion exchange chromatography is performed on diethylaminoethyl (DEAE)-Sepharose column and carboxymethyl (CM)-Sepharose column. In the production method, the medium includes 0.2-2.5% of soybean meal or casein as an organic nitrogen source, 0.2-2.5% of wheat flour or liquid maltose as a carbon source, and 0.2-2.5% of a low molecular weight carbon source chosen from sodium pyruvate, sodium citrate and sodium succinate with pH 8.0-11.0, and the culture is performed at 30-40degreesC with 200-800 rpm for 24-72 hours. The airflow condition is 0.5-1.5 vvm.Preparation: The protease (I) may be produced in E. coli by standard recombinant techniques or by chemical synthesis. ACTIVITY - Antimicrobial; Antibacterial; Virucide; Cytostatic; Vulnerary; Antiulcer; Anticoagulant; Thrombolytic; Hemostatic. No biological data given.    MECHANISM OF ACTION - None given.    EXAMPLE - Isolation of a strain producing a protease from the west coast mudflat was carried out as follows. Firstly, 1 g of the mudflat sample was suspended in 10 ml of sterilized distilled water, which was then left at room temperature for 5 minutes to precipitate shore components. Then, 0.2 ml of the supernatant was obtained and loaded on a agar plate containing 0.5% sodium carbonate and powdered skim milk, followed by observation on a clear zone formed around a colony formed by hydrolysis of the skim milk. Then, those colonies forming a clear zone in more than 2 mm diameter were selected. From the selected colonies, a strain producing a protease having an excellent activity was selected by using a liquid medium. The cell culture supernatant was recovered to examine the protease activity. Then, 0.5 ml of a substrate solution prepared by dissolving casein (final concentration: 0.5%) in 0.1 M glycine-NaOH buffer (pH 11.0) was mixed well with 0.1 ml of a 100-1000 fold diluted enzyme solution, followed by reaction at 60degreesC 10 minutes. Then, 0.5 ml of 10% trichloro acetic acid (TCA) was added to terminate the reaction. The reaction solution was put in ice for 10 minutes to precipitate proteins remaining unreacted. Supernatant was obtained by centrifugation and tyrosine was quantified at 275 nm. A strain showing the best protease activity was selected, which was confirmed to be a Gram-positive by the Gram-test. The morphological and biochemical characteristics of the selected strain were also investigated. The strain formed a spore and showed catalase and oxidase activities but did not show urease activity. The strain also produced acid and gas by using a sugar such as glucose, fructose, galactose, mannose, maltose, mannitol, sorbitol and starch, and proteins such as casein and gelatin. From a nucleotide sequencing, it was confirmed that the strain has 16S ribosomal RNA had a fully defined 695 base pair (SEQ ID No. 1) sequence given in the specification, and thus the strain was further confirmed to be a Bacillus sp. B.clausii strain. The selected strain was named B.clausii I-52 and deposited with accession no. KCTC 10277BP.