TECHNOLOGY FOCUS - BIOLOGY - Preferred Methods: Producing enzymatic products comprises adding cellulase-complex enzymes to dry biomass or wetted mass. The method further comprises: concentrating the partially hydrolyzed biomass and the cellulase complex mixture in water; allowing the biomass solids to settle in the water; recycling the enzyme complex; shearing and cavitating the organisms to disrupt the structure of the organisms to facilitate separation of mycelia and/or cell walls from cellulase enzyme complex, and to destroy the living cells; separating the cellulase enzyme from cellular material of the cellulase-producing organism; and pretreating the biomass for structural breakdown and increased surface area prior to adding the irradiated cellulase-producing organisms containing a cellulase complex, to the biomass. In the reconcentrating step: actively hydrolyzing the biomass and cellulase complex mixture, and mixing the concentrated biomass with other concentrated biomass in water; mechanically concentrating the biomass; adding additional irradiated cellulase complexes; and irradiating the concentrated biomass and cellulase complex. The recycling step includes: partially removing the cellulase enzyme complex from the biomass; irradiating the cellulase enzyme complex and attached organism cells; adding new biomass to the removed cellulase enzyme complex; and irradiating the combined biomass and removed cellulase enzyme complex. The separating step includes separating the enzyme using methods (shear and cavitation, breaking it down and mechanically separating). The pretreating step includes: irradiating the biomass prior to adding the irradiated cellulase-enzyme containing organisms to the biomass; and shearing and cavitating the biomass prior to adding the irradiated cellulase enzyme complex-containing organisms to the biomass. The active cellulase enzyme complex is isolated from the organisms, which is used in refining biomass to its components of carbohydrates, protein and lignin. The irradiating step includes: irradiating the cellulase-producing organism with radiation (0.0001-100 kGy) to destroy biological activity without destroying all cellulase enzyme complex activity; and mechanically extracting and concentrating actively-hydrolyzing biomass and a cellulase complex prior to irradiating the cellulase-producing organisms.Preferred Components: The enzymatic product is fermentable carbohydrates, plant leaf protein and lignin and the enzyme complex is cellulase enzyme complex. The biomass is a carbon-based material (preferably trees, grass, hay, sugar cane bagasse, crop residues, newsprint, paper, sewage sludge, aquatic plants or yard wastes). The system includes recycling means (continuous, fed-batch, semi-batch or batch processing) for recycling the biomass.