NOVELTY - A disposable kit (K1) (30) for treating bio-waste in a bio-waste containing article, comprising a batch of fluid activatable enzyme-producing bacteria (E-PB), where an amount of the enzyme produced is biologically effective to degenerate the bio-waste, and a biodegradable carrier, where the carrier is loaded with the batch of the bacteria.    USE - (K1) is useful for treating bio-waste in a bio-waste containing article, which involves providing (K1), placing (K1) at a desired location of a pre-soiled article, where the pre-soiled article is the bio-waste containing article before being loaded with the bio-waste, and treating the bio-waste by releasing the fluid activated enzyme from the bacteria. The pre-soiled article further comprises one or more absorbent layer, the desired location being at the one or more absorbent layer. The carrier is a biodegradable cellulose film or a time-release woven cellulose pack. The encasement is adhesively attached to the bio-waste containing article. The enzyme produced from the E-PB is timely released to the bio-waste containing article (claimed). (K1) is useful in treating wastewater such as municipal, food processing, and residential wastewater, for pre-treating wastewater containing high levels of oleaginous matter, for treating site sewage disposal systems such as septic tanks and cesspools, in agricultural uses for livestock feeding to increase food digestion and utilization, and for treating pipes, traps, plumbing systems and grease traps.    ADVANTAGE - (K1) is cost effective, and lessens the environmental problem.    DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a dispensing box (I) for dispensing one or more disposable bio-waste treatment kit, comprising a container sized and configured for stacking one or more of (K1).    DESCRIPTION OF DRAWING(S) - The figure shows an individually wrapped kit containing the enzyme-producing bacteria formed substrate.    disposable enzyme-producing bacteria kit (30)    tear or cut notch (34)    non-adhesive flap (42)    heat-sealing (44)    outer encasement (46)