TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: In (M1), the cotyledon from L. cylindrica is whole, not dissected one. The placing of inoculated cotyledon on a medium containing BAP is performed in such a manner that the total parts of the cotyledon are inserted into the medium layer. The amount of BAP in the culture medium is 1.0-4.0 mg/l, preferably 1.5-2.5 mg/l. The amount of NAA in the rooting medium is 0.001-0.08 mg/l, preferably 0.005-0.03 mg/l. ACTIVITY - Antipyretic; Gastrointestinal-Gen.; Antiinflammatory. No biological data is given.    MECHANISM OF ACTION - None given.    EXAMPLE - Luffa cylindrica Roem's seeds were sterilized for 15 minutes in 5% NaOCl solution and washed 3 times for 15 minutes with sterilized DW. The sterilized seeds were placed on germination media containing 1/2 Murashige and Skoog medium including minimal salts (MSMS), 2% sucrose and 0.6% agar and then cultured to germinate seed for 4 days at 25plus minus1degreesC under dark condition. Seedlings without true leaf were selected and cotyledon and hypocotyls were taken from them. A suitable composition was prepared with 16 combinations of cytokinin (6-benzylaminopurine (BAP)) and auxin (alpha-naphthalene acetic acid (NAA)). The basal media containing Murashige and Skoog medium cindluinkg Gamborg B5 vitamines (MSB5)), 500 mg/l of 2-(N-morpholino)ethanesulfonic acid monohydrate (MES), 3% sucrose and 0.6% agar were employed for regeneration. Each of 12 individuals was placed on each medium above and cultured for 30 days at 25plus minus1degreesC and 8000 lux under the condition of 16 hours/8 hours (light/dark). The regeneration rate was calculated from percentage of ratio of the number of regenerated section to total number of section placed and the average number of regenerated shoot was calculated from percentage of ratio of the number of regenerated shoot to the number of regenerated section. The regeneration rate and the ability in shoot formation were exhibited in the wide range of 6.67-40% and 0.49-1.0, respectively, depending on the media used. The hormone-combination experiment showed that at 1-4 mg/l concentration, BAP was found to accomplish higher regeneration rate and ability in shoot formation, while at more than 4 mg/l, to decrease regeneration rate. In the media containing 2 mg/l of BAP and no NAA, the highest regeneration rate were exhibited, the average number of shoot regenerated was found to be much larger (1.0/section) and the period necessary for regeneration was found to be excellent (approximately 20 days). All results above were obtained from cotyledon. As to hypocotyls, the regeneration rate was much lower, shoots were rarely regenerated, and callus formed were finally dead.