NOVELTY - Preparing a transformed Luffa cylindrica Roem, comprising inoculating a cotyledon from L. cylindrica Roem with Agrobacterium tumefaciens harboring a vector capable of inserting into a genome of a cell from L. cylindrica Roem, placing the inoculated cotyledon on a shoot medium containing 6-benzylaminopurine (BAP) and culturing regenerated shoots on a rooting medium containing alpha-naphthalene acetic acid (NAA), is new.    USE - (M1) is useful for preparing a transformed L. cylindrica Roem (claimed). The matured fibers of the transformed L. cylindrica Roem are useful in washing ships and decks and manufacturing slippers or baskets, useful as shoe mats, inner cloth of bonnet. The L. cylindrica Roem has effect on treatment of fever, enteritis, swelling, etc. The seed of L. cylindrica Roem containing 40% oil is useful as oil sources and the oil cake is useful as fertilizer and feed. The transformed L. cylindrica Roem is useful for the preparation of biologically important proteins.    ADVANTAGE - (M1) exhibits much higher transformation and regeneration efficiency leading to production of transformed L. cylindrica Roem having desirable traits with higher reproducibility.    DETAILED DESCRIPTION - Preparing (M1) a transformed Luffa cylindrica Roem, comprises:    (a) inoculating a cotyledon from L. cylindrica Roem with Agrobacterium tumefaciens harboring a vector, in which the vector is capable of inserting into a genome of a cell from L. cylindrica Roem and contains the sequences such as a replication origin operable in the cell from L. cylindrica Roem, a promoter capable of promoting a transcription in the cell from L. cylindrica Roem, a structural gene operably linked to the promoter and a polyadenylation signal sequence;    (b) placing the inoculated cotyledon on a medium containing 1.0-2.5 mg/l of 6-benzylaminopurine (BAP) by insert and culturing the inoculated cotyledon to obtain regenerated shoots; and    (c) culturing the regenerated shoots on a rooting medium containing 0.005-0.03 mg/l of alpha-naphthalene acetic acid (NAA) to obtain the transformed L.cylindrica Roem.    An INDEPENDENT CLAIM is also included for a transformed L. cylindrica Roem prepared by (M1).