NOVELTY - A formulation (I), comprising at least one polypeptide (P1) having phytase activity, where the polypeptide is chosen from a polypeptide encoded by a nucleic acid comprising a nucleotide sequence of a fully defined 1901 base pair (SEQ ID No. 7) sequence given in the specification or a polypeptide having an amino acid sequence of a fully defined 432 amino acid (SEQ ID No. 8) sequence given in the specification, is new.    USE - (I) is a dietary supplement useful for treating, preventing or reversing osteoporosis or bone loss, and preventing muscle cramps. The liquid supplement is useful for preventing muscle cramps. The method is useful for reducing pollution and increasing nutrient availability in an environment or environmental sample by degrading environmental phytic acid, where the environment or environmental sample comprises a soil or a body of water. The body of water is well, pond, lake, river, aquifer or reservoir. The environment or environmental sample comprises a sewage, sewage effluent, landfill or manure pond. (All claimed). The immobilized phytase is useful in foodstuffs for improving the feeding value of phytate rich ingredients.    DETAILED DESCRIPTION - A formulation (I), comprises at least one polypeptide (P1) having phytase activity, where (P1) is chosen from:    (a) a polypeptide encoded by a nucleic acid comprising a nucleotide sequence of a fully defined 1901 base pair (SEQ ID No. 7) sequence given in the specification, and where nucleotide 389 is G, 390 is A, 437 is T, 438 is G, 439 is G, 470 is C, 472 is T, 476 is T, 477 is G, 478 is T, 689 is G, 690 is A, 691 is G, 728 is T, 729 is A, 730 is T, 863 is T, 864 is G, or 1016 is G, or their combination, and the polynucleotide encodes a phytase;    (b) a polypeptide having an amino acid sequence of a fully defined 432 amino acid (SEQ ID No. 8) sequence given in the specification and having one or more amino acid modifications chosen from W68E, Q84W, A95P, K97C, S168E, R181Y, N226C, Y277D or their combination, where the polypeptide has phytase activity;    (c) a polypeptide encoded by a nucleic acid comprising a nucleotide sequence of a fully defined 1323 base pair (SEQ ID No. 1) sequence given in the specification;    (d) a polypeptide having an amino acid sequence of a fully defined 440 amino acid (SEQ ID No. 2) sequence given in the specification; or    (e) a combination of (a)-(d).    INDEPENDENT CLAIMS are also included for:    (1) a pharmaceutical composition (C1) comprising at least one (P1) and a excipient;    (2) a kit (K1) comprising (I) or (C1) and instructions for using (I) or (C1);    (3) an immobilized phytase (II) comprising (P1);    (4) a dietary supplement (III) comprising (II);    (5) a pharmaceutical composition (C2) comprising (II);    (6) a fertilizer or soil additive (IV) comprising (II), or at least one (P1);    (7) a liquid supplement (V) for preventing muscle cramps, comprising (I);    (8) a hydrating agent (VI), comprising (I);    (9) a tissue culture or cell culture media or cell culture media additive (VII) comprising at least one (P1);    (10) a plant food additive (VIII) comprising at least one (P1);    (11) reducing (M1) pollution and increasing nutrient availability in an environment or environmental sample by degrading environmental phytic acid, involves applying to the environmental or environmental sample an effective amount of a composition comprising at least one (P1).