NOVELTY - A biological synthetic composite (BSC) membrane comprises a porous support; and a functional phospholipid bilayer comprising at least one reconstituted functional membrane transport protein isolated from a cell. The porous support is polyethyleneterephthalate (PETE), polycarbonate (PC), poly-L-lactic acid (PLLA), and/or plastic plate comprising wells. Each well comprises at least one laser-drilled pore. The membrane transport protein is Kv1.5 K+ ion channel membrane transport protein and an H/K ATPase membrane transport protein.    USE - The membrane is used for high throughput screening assays useful for rapidly screening compounds for drug selection and design. The membrane is also used for fuel cells, biocides, toxin detectors and protective devices against toxins.    ADVANTAGE - The membrane has both sides accessible, and utilizes energy for the selective uptake, concentration, and/or release of ions and/or molecules in an organized manner.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for:    (1) method of making BSC membrane, comprising forming a functional lipid bilayer on a porous support; confirming existence of the functional lipid bilayer; reconstituting a membrane transport protein into the lipid bilayer; and confirming existence of the reconstituted transport protein; and    (2) method of identifying an agent as an inhibitor of a membrane ion transport protein, comprising forming a lipid bilayer on a porous support; measuring current across the bilayer in a medium comprising a membrane transport protein-permeable ion; reconstituting the membrane ion transport protein in the lipid bilayer; and measuring current across the bilayer in a medium comprising a membrane transport protein-permeable ion in the presence and absence of the agent, where current in the presence of an inhibitor of the ion transport protein is less than current in the absence of an inhibitor of the ion transport protein, in a medium comprising the membrane transport protein-permeable ion; and    (3) high throughput screening assay comprising the identifying method and being partially or fully automated.