TECHNOLOGY FOCUS - BIOTECHNOLOGY - Isolation (disclosed): The GXS1 DNA fragment was isolated from Candida intermedia.Preferred Host Cell: The host cell is a yeast, preferably Saccharomyces cerevisiae. EXAMPLE - The xylose/glucose active transporter from Candida intermedia was identified by comparison of the relative abundance of the proteins present in plasma membranes isolated from C.intermedia cultivated under inducing and repressing conditions, using Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein of about 40 kDa molecular weight that seems to be present only in plasma membranes of cells cultivated in 0.5% of xylose, corresponding to the molecular weight of sugar transporter, was isolated from preparative gel loaded with 250 micrograms of total membrane protein from C.intermedia cultivated in 0.5% of xylose. The sequencing of the N-terminal end of the isolated protein was carried out, and from this sequence, degenerated primers were designed. These primers were used to amplify the cDNA through Rapid amplification of cDNA ends (RACE) technique. The RNA sample from cells cultivated in 0.5% of xylose was used as template for the 3' RACE protocol, in combination with the CiGXSLl (5'-GARGAYAAYMGIATGGTIAARMG-3') and the CiGXSL2 (5'-AARMGITTYGTIAAYGTNGG-3') primers, in which I is inosine, Y is C/T, R is A/G, M is A/C and N=A/T/C. Since the design of the primers was based on the sequence of the first amino acids of the protein, it was expected that the 3' RACE reaction would produce the cDNA almost entirely. The 3' RACE reaction enables a product of about 1.7 kb, which was cloned in the pMOSBlue vector and partially sequenced, using an automatic sequencer ALF Express and Cy5-labelled primers specific for the vector sequences. The 5' end from the cDNA was obtained through the 5' RACE technique, using the CiGXSR3 (5'-CGTTAAGGAATGGAGCACAAAG-3') primer. The fragments obtained were cloned and sequenced, showing that an additional amino acid (initializing methionine) and a leader sequence of 28 or 31 amino acids were encoded, indicating the existence of two active sites of transcription initiation. Thus, novel gene designated GXSl (Glucose Xylose Symport 1) having base sequence of SEQ ID No. 1 was obtained.