TECHNOLOGY FOCUS - BIOLOGY - Preferred Components: The method further comprises providing the bacteria in the genus Pseudomonas stutzeri, Pseudomonas putida, Bacillus subtilis, Agrobacterium sp., Enterobacter cloacae, Zoogloea sp., Caulobacter vibrioides, Caulobacter sp. and/or Caulobacter crescentus to the container.    TECHNOLOGY FOCUS - ENVIRONMENT - Preferred Method: The method further comprises: inoculating the bacteria in the container; autoclaving the alginate solution prior to adding bacteria to the alginate solution; stirring the bacteria-alginate solution; separating the bacteria-alginate particles from the solution; introducing the preserved bacteria into a wastewater treatment system; introducing a flow of nutrients for the bacteria into the container; stopping a flow of nutrients for the bacteria into the container; allowing weaker bacteria to die and allowing hardier bacteria to enter a starvation phase; reviving the preserved bacteria; and reducing an amount of contaminant in the wastewater using the biofilm. The method further comprises introducing the bacteria to a first bacteria generator; adding a part of the preserved bacteria into a second bacteria generator; allowing the part of the preserved bacteria to incubate in the second bacteria generator; providing a portion of the bacteria in the second bacteria generator to a container comprises a substrate; and allowing the provided bacteria from the second bacteria generator to couple to the substrate in the container to form a biofilm. The preserving a portion of the bacteria comprises: mixing alginate into a first aqueous solution; adding the portion of the bacteria in the starvation phase to the alginate solution; adding the bacteria-alginate solution into a second aqueous solution comprising metal ions; allowing particles of bacteria-alginate to form; adding the nutrients to the preserved bacteria to revive the preserved bacteria; mixing alginate into an aqueous solution. The preserved bacteria is capable of entering a growth phase in the presence of nutrients.    TECHNOLOGY FOCUS - INORGANIC CHEMISTRY - Preferred Components: The bacteria-alginate solution is added drop wise into the second aqueous solution comprising metal ions. The second aqueous solution comprises calcium ions, barium ions, copper ions and/or zinc ions.