NOVELTY - Preparation of biological remover involves transferring activated sludge into bio-trickling filter and introducing hydrogen sulfide gas; taking 3 g acclimated filler inoculated with activated sludge, processing the diluted filler through ultrasonic wave vibration, smearing bacterial suspension on heterotrophic sulfur dioxide culture medium plate, and performing line-printing separation; and dividing the 2 strain of heterotrophic sulfur oxide psychrophile into two groups, culturing on heterotrophic sulfur dioxide culture medium plate, and mixing at a weight ratio of 1:1.    USE - Preparation method of biological remover for removing thiosulfate ion and sulfureted hydrogen under low temperature.    ADVANTAGE - Between 0-20degrees C, the maximal removing ratio of biological remover to thiosulfate ion is 62% while the maximal removing ratio of intermediate temperature heterotrophic mixed bacterium to thiosulfate ion is 29.8%. The maximal removing ratio of biological remover to sulfureted hydrogen at 0-20?OC, can reach 100%.    DETAILED DESCRIPTION - Preparation of biological remover for removing thiosulfate ion and sulfureted hydrogen under low temperature, involves:    (A) under 3-8 (preferably 5)degrees C, transferring activated sludge from sewage disposal plant into bio-trickling filter filled with active carbon, introducing hydrogen sulfide (H2S) gas at a rate 40-60 (preferably 50) mg/m3, increasing rate of H2S gas to 300-500 (preferably 500) mg/m3 in 30-90 (preferably 50) days, then using checking pipe to check air-out effect, if the air-out effect becomes stable the acclimation is finished;    (B) taking 3 g acclimated filler inoculated with activated sludge, diluting the filler by 100-110 (preferably 105) mL sterile water, processing the diluted filler through ultrasonic wave vibration for 10-30 (preferably 20) minutes to achieve bacterial suspension, in sterile room, taking 0.3-0.6 (preferably 0.6) mL bacterial suspension by sterile straw and smearing it on heterotrophic sulfur dioxide culture medium plate, inversely placing the heterotrophic sulfur dioxide culture medium plate in culture box, culturing under 4-6 (preferably 4)degrees C with constant temperature for 3-5 (preferably 4) days, performing line-printing separation to numerically superior colony on heterotrophic sulfur dioxide culture medium plate and further purifying, repeating the purifying step 3-5 times to achieve 2 strain of heterotrophic sulfur oxide psychrophile; and    (C) dividing the 2 strain of heterotrophic sulfur oxide psychrophile into two groups, culturing on heterotrophic sulfur dioxide culture medium plate respectively for 3-5 days, mixing the two heterotrophic sulfur oxide psychrophile according to weight ratio of 1:1, washing with sterile water and adding distilled water to achieve heterotrophic sulfur oxide psychrophile suspension (1g psychrophile/40-50mL distilled water).