TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: The cellobiohydrolase, endoglucanase, or beta-glucosidase, is obtainable from Thermoascus aurantiacus, Acremonium thermophilum, or Chaetomium thermophilum, where the cellobiohydrolase, endoglucanase, or beta-glucosidase, is obtainable from T. aurantiacus CBS 116239, A. thermophilum CBS 116240, or C. thermophilum CBS 730.95. Recombinant enzymes are used, preferably produced in a strain from the genus Trichoderma or Aspergillus, where the method comprises using a cellobiohydrolase obtainable from T. aurantiacus or A. thermophilum to which a cellulose binding domain has been genetically attached, and where the attached cellulose binding domain is derived from Trichoderma reesei or C. thermophilum, and preferably the resulting fusion protein comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 28 or 30. The endoglucanase comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 10, 12, 14 or 16, or to its enzymatically active fragment. The beta-glucosidase comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 22, 24 or 26, or to its enzymatically active fragment. The cellulosic material is lignocellulosic material. The method comprises treating lignocellulosic material with at least one further enzyme, preferably a xylanase, which preferably comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 18 or 20, or to its enzymatically active fragment, where the xylanase is obtainable from T. aurantiacus or A. thermophilum, preferably from T. aurantiacus CBS 116239, or A. thermophilum CBS 116240. The enzymes are added to the cellulosic material either simultaneously or sequentially. At least one of the enzymes is encoded by a gene similar to that included in a microorganism having accession number DSM 16723, DSM 16728, DSM 16729, DSM 16727, DSM 17326, DSM 17324, DSM 17323, DSM 17729, DSM 16724, DSM 16726, DSM 16725, DSM 17325 or DSM 17667. The cellulosic material is corn stover, switchgrass, cereal straw, sugarcane bagasse, or wood derived materials. Treating cellulosic material with a spent culture medium of at least one microorganism capable of producing a polypeptide comprising a fragment having cellulolytic activity described above comprises reacting the cellulosic material with the spent culture medium to obtain hydrolyzed cellulosic material. Preparing a polypeptide comprising a fragment having cellulolytic activity described above comprises transforming a host cell with a vector encoding the polypeptide, and culturing the host cell under conditions enabling expression of the polypeptide, and optionally recovering and purifying the polypeptide produced.Preferred Enzyme Preparation: The enzyme preparation is in the form of spent culture medium, powder, granules, or liquid. An enzyme preparation comprises a polypeptide above. The enzyme preparation comprises cellobiohydrolase, endoglucanase, beta-glucosidase, and optionally xylanase activity and/or other enzyme activities. The enzyme preparation further comprises conventional additives.Preferred Vector: The vector is capable of expressing a polypeptide above.Preferred Host Cell: The host cell is capable of expressing the polypeptide encoded by the heterologous polynucleotide sequence, where the host cell is a strain from the genus Trichoderma or Aspergillus. EXAMPLE - No suitable example given.