NOVELTY - Raw amylolytic enzyme I or II obtained by suspending powdery Eisenia fetida in 50 mM tris hydrochloric acid, collecting supernatant liquids after centrifuging for 20 minutes at 15000 rpm and at 4degrees C, adding protease inhibitor to supernatant liquid, adding saturated ammonium sulfate to supernatant, collecting precipitates after centrifugation, dialyzing the enzyme liquid using cellulose tube, fractionating active fraction obtained by applying to anion exchange chromatography and to gel-filtration chromatography, obtaining enzyme active fraction, is new.    USE - The raw amylolytic enzyme I or II is useful for alcoholic fermentation (claimed), and in foodstuffs and for processing waste material containing raw starch.    ADVANTAGE - The raw amylolytic enzyme I or II enables processing waste material containing raw starch, at low temperature.    DETAILED DESCRIPTION - Raw amylolytic enzyme I or II isolated from Eisenia fetida by (a) freeze-drying Eisenia fetida obtained from food overnight, (b) suspending powdery Eisenia fetida grinded by mortar in 50 mM tris hydrochloric acid (pH 7.0), (c) collecting supernatant liquids after centrifuging for 20 minutes at 15000 rpm and at 4degrees C, (d) adding protease inhibitor to the supernatant liquid, (e) collecting supernatant liquids after carrying out ultracentrifugation for 30 minutes at 100000 G and at 4degrees C, (f) adding saturated ammonium sulfate (35%) in the supernatant liquid, (g) maintaining the liquid at 4degrees C overnight, (h) then, collecting the precipitates after centrifuging for 20 minutes at 15000 rpm and at 4degrees C, (i) dialyzing the enzyme liquid obtained by dissolving the obtained precipitate in a small amount of 50 mM tris hydrochloric acid (pH 7.0) using the cellulose tube for dialysis with respect to the liquid containing 20 mM tris hydrochloric acid (pH 7.0), the decomposition activity with respect to starch or raw starch is used as a parameter for the liquid in the dialysis, (j) fractionating active fraction eluted earlier or later among two active fractions obtained by applying to the anion exchange chromatography by DEAE-TOYOPEARL (RTM: Not defined) 650 M, (k) fractionating active fraction by subsequently applying the fraction to the gel-filtration chromatography using Sephacryl S-200, (l) applying the active fraction to the hydrophobic chromatography using BUTYL-TOYOPEARL (RTM: Not defined) 650S, and (m) obtaining the raw amylolytic enzyme active fraction by fractionating the active fraction. An INDEPENDENT CLAIM is included for alcoholic fermentation method, involves saccharifying a starch with the above-cited raw amylolytic enzyme, and cultivating yeast in the saccharide.