TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Composition: In the composition, the ester substrate is a liquid and the perhydrolase enzyme and hydrogen peroxide source are solids suspended in the liquid. The peracid is selected from peracetic acid, pernonanoic acid, perpropionic acid, perbutanoic acid, perpentanoic acid, and perhexanoic acid, where the peracid is peracetic acid. The ester substrate is propylene glycol diacetate. The perhydrolase enzyme comprises the amino acid sequence of fully defined 216 amino acids (SEQ ID NO: 1), given in the specification. The perhydrolase enzyme is the S54V variant of SEQ ID NO: 1. The hydrogen peroxide source is a hydrogen peroxide generating compound selected from sodium percarbonate, sodium perborate, and urea hydrogen peroxide. The hydrogen peroxide source is sodium percarbonate. The composition comprises about 0.001-1% by weight perhydrolase enzyme, about 35-45% by weight sodium percarbonate, and about 55-65% by weight propylene glycol diacetate. Moreover, the hydrogen peroxide source is an enzymatic hydrogen peroxide generation system comprising an oxidase and its substrate, where the oxidase is selected from glucose oxidase, sorbitol oxidase, hexose oxidase, choline oxidase, alcohol oxidase, glycerol oxidase, cholesterol oxidase, pyranose oxidase, carboxyalcohol oxidase, L-amino acid oxidase, glycine oxidase, pyruvate oxidase, glutamate oxidase, sarcosine oxidase, lysine oxidase, lactate oxidase, vanillyl oxidase, glycolate oxidase, galactose oxidase, uricase, oxalate oxidase, and xanthine oxidase. The composition also comprises at least one additional enzyme selected from proteases, cellulases, amylases, and microbial cell wall degrading enzymes. The composition further comprises at least one surfactant. Preferred Method: In decontaminating, the composition is contained in a water soluble container, where the container is a sachet made of polyvinyl alcohol film. The water is sterilized. Moreover, exposing comprises exposing the item to the solution at high temperature. The contaminant comprises a toxin selected from botulinum toxin, anthracis toxin, ricin, scombroid toxin, ciguatoxin, tetrodotoxin, mycotoxins, and any combination. The contaminant comprises a pathogen selected from bacteria, viruses, fungi, parasites, prions, and any combination. The pathogen is selected Bacillus spp., B. anthracis, Clostridium spp., C. botulinum, C. perfringens, Listeria spp., Staphylococcus spp., Streptococcus spp., Salmonella spp., Shigella ssp., Escherichia coli, Yersinia spp., Y. pestis, Francisella spp., F. tularensis, Campylobacter ssp., Vibrio spp., Brucella spp., Cryptosporidium spp., Giardia spp., Cyclospora spp., and Trichinella spp. The item is selected from hard surfaces, fabrics, food, feed, apparel, rugs, carpets, textiles, medical instruments, and veterinary instruments. Preferred System: In the system, the container is a sachet, ampoule, capsule, or sphere. The water soluble container is made of a water soluble polymer material polyvinyl alcohol, polyethylene oxides, cellulose derived water soluble carbohydrates, and polylactides. Preferred Kit: The kit also comprises an empty mixing container for mixing the composition with water; water in a sealed container, where the water is sterilized; and instructions for use in a method. SPECIFIC SEQUENCES - Specifically claimed is perhydrolase enzyme amino acid sequence comprising 216 amino acids (SEQ ID NO: 1), fully defined in the specification.    EXAMPLE - No suitable example given.