TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Process: Separation comprises distillation, where the separating further comprises at least one step selected from evaporation, gas-stripping, adsorption, and liquid-liquid extraction. Making a reaction product comprising at least one isooctane comprises:(A) obtaining a fermentation broth comprising 1-butanol;(B) separating dry 1-butanol from the fermentation broth to form separated dry 1-butanol;(C) contacting the separated dry 1-butanol of step (b), optionally in the presence of a solvent, with at least one acid catalyst at 50-450degrees C and a pressure of 0.1-20.7 MPa to produce a first reaction product comprising at least one isooctene;(D) recovering the at least one isooctene from the first reaction product to obtain at least one recovered isooctene;(E) contacting the at least one recovered isooctene with hydrogen in the presence of at least one hydrogenation catalyst to produce the reaction product comprising at least one isooctane; and(F) optionally recovering the at least one isooctane from the reaction product to obtain at least one recovered isooctane.Making a reaction product comprising at least one isooctanol comprises:(A) obtaining a fermentation broth comprising 1-butanol;(B) separating dry 1-butanol from the fermentation broth to form separated dry 1-butanol;(C) contacting the separated dry 1-butanol of step (b), optionally in the presence of a solvent, with at least one acid catalyst at 50-450degrees C and a pressure of 0.1-20.7 MPa to produce a first reaction product comprising at least one isooctene;(D) recovering the at least one isooctene from the first reaction product to obtain at least one recovered isooctene;(E) contacting the at least one recovered isooctene with water and at least one acid catalyst to produce the reaction product comprising at least one isooctanol; and(F) optionally recovering the at least one isooctanol from the reaction product to obtain at least one recovered isooctanol.Making a reaction product comprising at least one isooctyl alkyl ether comprises:(A) obtaining a fermentation broth comprising 1-butanol;(B) separating dry l-butanol from the fermentation broth to form separated dry l-butanol;(C) contacting the separated dry 1-butanol of step (b), optionally in the presence of a solvent, with at least one acid catalyst at 50-450degrees C and a pressure of 0.1-20.7 MPa to produce a first reaction product comprising at least one isooctene;(D) recovering the at least one isooctene from the first reaction product to obtain at least one recovered isooctene;(E) contacting the at least one recovered isooctene with at least one straight-chain or branched C1 to C5 alcohol and at least one acid catalyst to produce the reaction product comprising at least one isooctyl alkyl ether; and(F) optionally recovering the at least one isooctyl alkyl ether from the reaction product to obtain at least one recovered isooctyl alkyl ether.Making at least one isooctane comprises:(A) obtaining a fermentation broth comprising 1-butanol;(B) separating dry 1-butanol from the fermentation broth to form separated dry 1-butanol;(C) contacting the separated dry 1-butanol of step (b), optionally in the presence of a solvent, With at least one acid catalyst at 50-450degrees C and a pressure of 0.1-20.7 MPa to produce a first reaction product comprising at least one isooctene;(D) contacting the first reaction product with hydrogen in the presence of at least one hydrogenation catalyst to produce a second reaction product comprising at least one isooctane; and(E) recovering the at least one isooctane from the second reaction product to produce a recovered isooctane. EXAMPLE - No suitable example given.