TECHNOLOGY FOCUS - ENVIRONMENT - Preferred Components: The fillers in anoxic zone and aerobic zone are bunchy semiflexible fiber fillers. The domestication culture medium is LB liquid domestication culture medium with nutrient content of 1/4, 1/8 and 1/16 of standard LB liquid culture medium. The substitute waste water is mixed with distilled water and domestication culture medium is made upto 100% waste water. Preferred Method: The settled sludge in settler is divided into two portions, where one portion of the settled sludge is refluxed to anoxic zone at a rate of 1.5-2.2, and the other portion is exhausted. The settled sludge is refluxed in anoxic zone of biological treatment device for 8-12 days. The waste water is pretreated before introducing into biological treatment device. The recombinant bacteria is obtained by removing impurity from sludge in aerated tank of waste water treatment plant, and culturing the sludge at 10degrees C such that the chemical oxygen demand (COD) removing rate is 85%, crushing sludge floe to carry out gradient diluting and culturing at 10degrees C, separating and purifying the cultured materials, performing domestication culturing at 10degrees C, selecting 2-8 microorganisms with short and high turbidity degree, mixing both microorganisms, and collecting the mixture containing both cultures. The recombinant bacteria is introduced into system by spraying the recombinant bacteria on filler surface symmetrically, introducing the bacteria into anoxic zone and aerobic zone, in a ratio of 1:8-12, passing waste water after 1-5 days in batch type, aerating for 22 hours, maintaining the aerated contents for 2 hours in 1-2 days, aerating the contents for 10 hours, maintaining the aerated contents for 2 hours in 3-5 days, spraying the recombinant bacteria (1-2 vol.%) culture solution into the treatment device on day 1, 4 and 6, spraying urea into waste water on day 4-6, flowing waste water on day 6 for 8-10 hours, refluxing sludge at a ratio of 1.5-2.0 until the sludge consistency is higher than 2000mg/l, and total quantity of bacteria is more than 1000000/ml, and COD removing rate is 80%, where the urea spraying quantity is five days biochemical oxygen demandx daily treated water quantityx 0.157/1000. In the step of introducing waste water in batch type, the aeration volume of batch type is 4mg/l. Preferred Ratio: The volume ratio of oxygen charging aerated gas and water in aerobic zone is 10-15:1.