NOVELTY - In situ biological remediation of pollution of biomass strengthening oil to soil, by adding petroleum hydro-carbons decomposing and combining bacterial agents to soil contaminated with hydrocarbons, producing biosurfactants and intermediary secondary metabolites as a part of glucide, to activate petroleum hydro-carbons decomposition and for resisting the ecological reason which limits the biological remediation and biological factors.    USE - The method is useful for in situ biological remediation method of pollution of biomass strengthening oil to soil (claimed).    ADVANTAGE - The method is simple and easy to operate. The method promotes efficient bioremediation of hydrocarbons, and the byproducts are effectively used for improving soil fertilization.    DETAILED DESCRIPTION - An in situ biological remediation method of pollution of biomass strengthening oil to soil, involves forming intermediate product in the process of transformation of biomass in soil to promote quick growth of petroleum hydro-carbons decomposing and combining bacterial agents in soil to overcome ecological reason which limits in situ biological remediation and non-biological factors, adding petroleum hydro-carbons decomposing and combining bacterial agents to the contaminated soil, where the petroleum hydro-carbons decomposing and combining bacterial agents contain bacteria which can produce biosurfactants and intermediary secondary metabolites as a part of glucide which is obtained by transforming biomass decomposing bacteria. The glucide promotes the growth of petroleum hydro-carbons decomposing and combining bacterial agents. The bacterial agents contain microorganism which produces biosurfactants with high efficiency in growth to produce large quantity of biosurfactants to activate petroleum hydro-carbons contamination in contaminated soil and improve the extraction of petroleum hydro-carbons decomposing and combining bacterial agents by microorganism and resist the ecological reason which limits the biological remediation and biological factors.