NOVELTY - Treating cellulosic material with cellobiohydrolase, endoglucanase and beta-glucosidase, where the cellobiohydrolase comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 2, 4, 6 or 8, or to its enzymatically active fragment.    USE - The enzyme preparation is useful for degrading cellulosic material, where the cellulosic material is corn stover, switchgrass, cereal straw, sugarcane bagasse, or wood derived materials. Treatment method is useful for preparing ethanol from cellulosic material. The polypeptide or enzyme preparation is useful in fuel, textile, detergent, pulp and paper, food, feed or beverage industry, where the enzyme is used in treatment of kraft pulp, mechanical pulp, or recycled paper, and where the enzyme preparation is spent culture medium (all claimed).    ADVANTAGE - The present invention provides method for degrading cellulosic substrates, in particular lignocellulosic substrates, and with enhanced efficiency. IT also provides processes and enzymes, which work at high temperature, thus enabling the use of high biomass consistency and leading to high sugar and ethanol concentrations, and thus leads to significant saving in energy and investment costs. The high temperature also decreases the risk of contamination during hydrolysis.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are:    (1) an enzyme preparation comprising cellobiohydrolase, endoglucanase and beta-glucosidase, where the cellobiohydrolase comprises an amino acid sequence having at least 80% identity to SEQ ID NO: 2, 4, 6 or 8, or to its enzymatically active fragment;    (2) a polypeptide comprising a fragment having cellulolytic activity and is selected from:    (i) a polypeptide comprising an amino acid sequence having at least 66% identity to SEQ ID NO: 4, 79% identity to SEQ ID NO: 6, 78% identity to SEQ ID NO: 12, 68% identity to SEQ ID NO: 14, 72% identity to SEQ ID NO: 16, 68% identity to SEQ ID NO: 20, 74% identity to SEQ ID NO: 22 or 24, or 78% identity to SEQ ID NO: 26,    (ii) a variant of (i) comprising a fragment having cellulolytic activity, or    (iii) a fragment of (i) or (ii) having cellulolytic activity;    (3) an isolated polynucleotide selected from    (i) a nucleotide sequence of SEQ ID NOS: 3, 5, 11, 13, 15, 19, 21, 23 or 25, or a sequence encoding a polypeptide,    (ii) a complementary strand of (i),    (iii) a fragment of (i) or (ii) comprising at least 20 nucleotides, and    (iv) a sequence that is degenerate as a result of the genetic code to any one of the sequences in (i)-(iii);    (4) a vector, which comprises as a heterologous sequence a polynucleotide;    (5) a host cell comprising the vector;    (6) an Escherichia coli strain having accession number DSM 16728, DSM 16729, DSM 17324, DSM 17323, DSM 17729, DSM 16726, DSM 16725, DSM 17325 or DSM 17667; and    (7) a method for preparing a polypeptide.