NOVELTY - Biological method designed to speed the overall biological oxidation removal rate of organic waste matter within any waste water liquid containment system, comprises using a liner within the containment system to establish a lined aquatic environment, where the liner has a surface, pre-coating the surface with attached microorganisms that will germinate and grow to establish necessary bio films on the liner surface and contacting the surface with liquid waste containing organic matter.    USE - The invention deals about the biological method designed to speed the overall biological oxidation removal rate of organic waste matter within any waste water liquid containment system e.g. ponds, lakes or waste holding. The method is useful for protecting the residential and commercial aquatic environment. The method is useful for water clarification and purification.    ADVANTAGE - The method: reduces organic pollutants and the time for biological function to occur; improves the rate of biological removal efficiency; and provides better support for aquatic life forms.    DETAILED DESCRIPTION - Biological method designed to speed a biological oxidation removal rate of organic waste matter within any waste water liquid containment system, comprises using a liner within the containment system to establish a lined aquatic environment, where the liner has a surface, pre-coating the surface with attached microorganisms that will germinate and grow to establish necessary bio films on the liner surface, and contacting the surface with liquid waste containing organic matter, where: the liner organisms will then grow, multiply and seed the water with additional microorganisms to increase the biological removal efficiency of the waste water contained within the lined aquatic environment; and the microorganisms are applied to the liner in a dry biological formulation. An INDEPENDENT CLAIM is included for a method of pre colonizing a water containment area having impurities comprising attaching microbial spore forming microorganisms to the liner to be placed within the containment area by precoating the liner; allowing the pre coated liner to dry and packaging for introducing the pre coated liner from the package into the water containment area that contains waste organic matter and for activating the microorganisms to germinate and begin to produce additional microbial colonies.