TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: Producing a biofuel also comprises alkylating cysteine residues present within the lipase which is modified to comprise a steroidal moiety. The lipase is selected from a Thermomyces lanuginosus lipase, a Candida Antarctica Lipase B, a phospholipase C beta , optionally a phospholipase C beta 3, and their combinations. The phospholipase C beta 3 is a mammalian phospholipase C beta 3. The mammalian phospholipase C beta 3 is a human phospholipase C beta 3 that comprises SEQ ID NO: 1 not given in the specification, or a biologically active fragment or its variant. The cysteine residues are selected from C193, C221, C360, C516, C614, C892, C1176, and C1207 of SEQ ID NO: 1 not given in the specification. The lipase is an avian phospholipase C beta , optionally a turkey phospholipase C beta comprising an amino acid sequence as set forth in SEQ ID NO: 2, or a biologically active fragment or its variant. The lipase is present in a cell free system comprising mixed micelles. The alkylating comprises contacting the lipase with an alkylating agent, optionally U73122 (1-(6-(((17 beta )-3-methoxyestra-1 ,3,5(10)-trien-17-yl)amino)hexyl)-2,5-pyrrolidinedione) or its analog, under conditions and for a time sufficient that at least one, optionally one to eight, cysteine residue(s) on the lipase is/are alkylated. The lipase is preferably bound to a surface. In enhancing a biological (e.g., catalytic) activity of a lipase, the lipase is modified to comprise a steroidal moiety. The lipase is an avian phospholipase C beta , optionally a turkey phospholipase C beta comprising SEQ ID NO: 2 not given in the specification, or a biologically active fragment or its variant. The lipase is present in a cell free system comprising mixed micelles. The biological (e.g., catalytic) activity of the lipase is enhanced two-, three-, four- or more-fold as compared to the corresponding lipase that has not been alkylated. Preferred System: In the system, a biological activity of the lipase is enhanced relative to the same system in which the lipase does not comprise any such moieties. The system is a cell-free system. The surface is provided on a particle, sheet, plate, or the like. Preferred Lipase: In the isolated modified lipase, the steroidal moieties, optionally U73122 moieties or U73122 analog moieties, are bound to one, two, three, four, five, six, seven, eight, or more cysteine residues present in the modified lipase. The moieties may also be bound to one, two, three, four, five, six, seven, eight, or more cysteine residues corresponding to the cysteine residues e.g. C193, C221, C360, C516, C614, C892, C1176, and C1207 of SEQ ID NO: 1. The lipase also comprises SEQ ID NO: 1, or its functional fragment, and further where at least one of C193, C221, C360, C516, C614, C892, C1176, and C1207, if present, is conjugated to a steroidal moiety, optionally a U73122 moiety or U73122 analog moiety. EXAMPLE - No suitable example given.