TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Component: The bacterium is Arthrobacter aurescens strain (NBRC 12136), Arthrobacter citreus strain (NBRC 12957), Arthrobacter histidinolovorans strain (JCM 2520), Arthrobacter nicotianae strain (JCM 1333), Arthrobacter protophormiae strain (JCM 1973), Arthrobacter globiformis strain (ATCC 4336), Arthrobacter pascens strain (ATCC 13346), Arthrobacter paraffineus strain (ATCC 21003), Bacillus badius strain (ATCC 14574), Bacillus sphaericus strain (NBRC 3341), Bacillus licheniformis strain (NBRC 12197), Bacillus cereus var. juroi strain (ATCC 21182), Microbacterium barkeri strain (JCM 1343), Microbacterium testaceum strain (JCM 1353), Microbacterium liquefaciens strain (JCM 3879), Microbacterium esteraromaticum strain (NBRC 3751), Raoultella planticola strain (JCM 7251), Raoultella terrigena strain (JCM 1687), Archromobacter denitrificans strain (NBRC 12669), Achromobacter lyticus strain (ATCC 21456), Brevibacterium butanicum strain (ATCC 21196), Brevibacterium ketoglutamicum strain (ATCC 21004), Brevibacterium fuscum strain (JCM 1488), Corynebacterium glutamicum strain (ATCC 14020), Curtobacterium albidum strain (JCM 1344), Curtobacterium luteum strain (JCM 1480), Curtbacterium citreum strain (JCM 1345), Devosia riboflavina strain (NBRC 13584), Exiguobacterium acetylicum strain (JCM 1968), Frateuria aurantia strain (NBRC 3247), Hafnia alvei strain (NBRC 3731), Mesorhizobium loti strain (NBRC 13336), Nocardioides simplex strain (NBRC 12069), Paenibacillus validus strain (NBRC 13635), Paenibacillus polymyxa strain (NBRC 3020), Paenibacillus macerans strain (JCM 2500), Proteus vulgaris strain (NBRC 3851), Saccharopolyspora erythraea strain (NBRC 13426), Spirillospora albida strain (NBRC 12248), Streptomyces parvulus strain (NBRC 13193), Streptomyces virginiae strain (NBRC 12827), Streptomyces galilaeus strain (ATCC 31133), Streptomyces canus strain (ATCC 12646), Streptomyces fradiae strain (ATCC 10745), Streptomyces alboflavus strain (NBRC 13196), Streptomyces albovinaceus strain (NBRC 12739), Streptomyces cellulosae strain (NBRC 3713), Streptomyces fumanus strain (NBRC 13042), Streptomyces longispororuber strain (NBRC 13488), Pseudomonas azotoformans strain (JCM 2777), Pseudomonas oryzihabitans strain (JCM 2952), Pseudomonas synxantha strain (NBRC 3913), Pseudomonas fragi strain (JCM 20552), Pseudomonas chlororaphis strain (NBRC 3904), Pseudomonas taetrolens strain (NBRC 3460), Nocardia uniformis strain (NBRC 13702), or Citrobacter freundii strain (JCM 1657). Preferred Condition: The bacterium is cultured under an aerobic condition. EXAMPLE - A Citrobacter freundii strain (JCM 1657) was spread on a plate culture agar medium F comprising (g) potassium phosphate monobasic (3), sodium phosphate dibasic (6), sodium chloride (0.5), ammonium chloride (1), magnesium sulfate heptahydrate (0.25), glycerol (20), agar (20), and distilled water (1 L) with final pH of 7.4. The bacterial strain was allowed to stand at 30 degrees C for 4 days. The bacterial strain grown on the plate was inoculated with a platinum loop in a test tube culture medium G (3 mL) containing same composition with medium F but with no agar and containing glycerol (10 g); and subjected to shaking culture at 30 degrees C at 200 rpm for 24 hours. The grown bacterial strain culture solution (30 mu L) was transferred to another test tube culture medium H (3 mL) containing same composition with medium G and a glycerol fraction (19.6 g) comprising 51% glycerol, 11% methanol, 8% potassium hydroxide, 4% water, and 26% glyceride which was by-product from biodiesel fuel production. The bacterial strain culture solution was then subjected to shaking culture at 300 degrees C at 200 rpm for 24 hours. Four days after initiation of the reaction, 4.2 g/L of pyruvic acid was accumulated.