NOVELTY - Electrochemically active strains separation involves shearing anode of microbial fuel cell under bacteria-free condition i.e. by adding disinfecting liquid. The disinfecting liquid is diluted and transferred to Hungate roll tube and dispersed in a solid culture medium for culturing at 28-31 degrees C for 5-10 days. A strain colony is selected from Hungate roll tube and inoculated in a liquid culture medium, and cultured for 2-4 days at 25-32 degrees C to obtain a strain with electrochemical active strain.    USE - Method for separating electrochemically active strains (claimed).    ADVANTAGE - The electrochemically active strains separation method separates strains having simple nutritional value and high electrochemical activity.    DETAILED DESCRIPTION - Electrochemically active strains separation involves shearing anode of microbial fuel cell under bacteria-free condition, where disinfecting liquid is added, diluted and transferred to Hungate roll tube and dispersed in a solid culture medium for culturing. A strain colony is selected from Hungate roll tube and inoculated in a liquid culture medium, and cultured for 2-4 days at 25-32 degrees C to obtain a strain with electrochemical active strain. 1000 ml of liquid culture medium comprises 10-20g of glucose, 2-4g of tryptone, 2-4 g of beef extract, 1-1.5g of yeast, 3-6 g of sodium chloride, 1-2 g of dipotassium hydrogen phosphate, 0.1-0.3 g of magnesium chloride, 0.1-0.25 g of ferrous sulfate heptahydrate, 3-7 ml of vitamin containing liquid, 10-14 ml of trace element containing liquid and 0.05 mol of phosphate buffered saline at pH 7. 1000 ml of solid culture medium comprises 10-20 g of agar, 10-20 g of glucose, 2-4 g of tryptone, 2-4 g of beef extract, 1-1.5 g of yeast, 3-6 g of sodium chloride, 1-2 g of dipotassium hydrogen phosphate, 0.1-0.3 g of magnesium chloride, 0.1-0.25 g of ferrous sulfate heptahydrate, 3-7 ml of vitamin containing liquid, 10-14 ml of trace element containing liquid and 0.05 mol of phosphate buffered saline at pH 7. An INDEPENDENT CLAIM is included for a testing method for electrochemically active strains, which involves:    (A) adding strain containing liquid in an aqueous solution; and    (B) mixing the aqueous solution into a reactor and installing a cyclic voltmeter-ammeter for measuring electric potential.