TECHNOLOGY FOCUS - BIOTECHNOLOGY - Isolation (Claimed): The polynucleotide is isolated from bacterium, plant, insect, yeast, fungus, or mammal, preferably bacterium. The bacterium is Gram positive or Gram negative bacterium. The bacterium is isolated from the genus Jeotgalicoccus. The bacterium is chosen from Jeotgalicoccus halotolerans, Jeotgalicoccus psychrophilus, Jeotgalicoccus sp. ATCC 8456, Jeotgalicoccus pinnipedalis, Corynebacterium efficiens, Kokuria rhizophila, Methylobacterium populi, and Bacillus subtilis. Preferred Polynucleotide: The nucleotide sequence exhibits at least about 80% (preferably 90% or 95%) sequence identity to SEQ ID No. 1, 4, 6, 8, 9, 11 or 13. The biological activity is fatty acid decarboxylase activity. The isolated polynucleotide further comprises an operably linked promoter. Preferred Vector: The recombinant vector is a plasmid. Preferred Host: The host cell is chosen from mammalian cell, plant cell, insect cell, yeast cell, fungus cell, filamentous fungi cell, and bacterial cell. The host cell is chosen from the genus Escherichia, Bacillus, Lactobacillus, Rhodococcus, Pseudomonas, Aspergillus, Trichoderma, Neurospora, Fusarium, Humicola, Rhizomucor, Kluyveromyces, Pichia, Mucor, Myceliophtora, Penicillium, Phanerochaete, Pleurotus, Trametes, Chrysosporium, Saccharomyces, Stenotrophamonas, Schizosaccharomyces, Yarrowia, and Streptomyces. The host cell is (a) Bacillus lentus cell, Bacillus brevis cell, Bacillus stearothermophilus cell, Bacillus licheniformis cell, Bacillus alkalophilus cell, Bacillus coagulans cell, Bacillus circulans cell, Bacillus pumilis cell, Bacillus thuringiensis cell, Bacillus clausii cell, Bacillus megaterium cell, Bacillus subtilis cell, and Bacillus amyloliquefaciens cell, (b) Trichoderma koningii cell, Trichoderma viride cell, Trichoderma reesei cell, Trichoderma longibrachiatum cell, A.awamori cell, A.fumigates cell, A.foetidus cell, A.nidulans cell, A.niger cell, A.oryzae cell, Humicola insolens cell, Humicola lanuginose cell, Rhodococcus opacus cell, Rhizomucor miehei cell, or Mucor miehei cell, (c) Streptomyces lividans cell or Streptomyces murinus cell, (d) Actinomycetes cell, (e) Chinese hamster ovarian (CHO) cell, COS cell (cell line derived from kidney cells of African green monkey), VERO cell, BHK cell, HeLa cell, CvI cell, Madin-Darby canine kidney (MDCK) cell, 293 cell, 3T3 cell, or PC12 cell (cell line derived from a pheochromocytoma of the rat adrenal medulla), (e) Escherichia coli cell including strain B, a strain C, a strain K, or strain W E.coli cell. The host cell expresses a polypeptide encoded by the recombinant vector. The polynucleotide sequence is stably incorporated into the genomic DNA of the host cell, and where the expression of the polynucleotide sequence is under the control of a regulated promoter region. The host cell produces an olefin. The olefin is a terminal olefin. The olefin is secreted by the host cell. The host cell expresses a reduced level of an acyl-CoA synthase relative to a corresponding wild-type host cell. The gene encoding an acyl-CoA synthase is attenuated or deleted in the host cell. The gene is Fas (TNFRSF6)-associated via death domain (fadD), JadK, BH3103, yhflL, Pfl-4354, EAV15023, fadDl, fadD2, RPC4074, fadDD35, fadDD22, faalp, faa3p, lcfA, or the gene encoding the protein having the amino acid sequence (ZP 1644857). The gene is fadD. The gene encoding an acyl-Co A synthase is attenuated or deleted in the microorganism. Preferred Microorganism: The polynucleotide is endogenous to the microorganism. Preferred Method: The method further involves culturing the host cell in the presence of at least one biological substrate for the polypeptide. The terminal olefin comprises 5-25C terminal olefin, preferably 13-21C terminal olefin. The terminal olefin comprises an unsaturated terminal olefin, monounsaturated terminal olefin, straight chain terminal olefin, branched chain terminal olefin or cyclic moiety. The substrate is a fatty acid. The fatty acid comprises 6-26C fatty acid or 14-22C fatty acid. The fatty acid is chosen from tetradecanoic acid, hexadecanoic acid, octadecanoic acid, behenic acid, methyl eicosanoic acid, eicosanoic acid, methyl octadecanoic acid, stearic acid and palmitic acid. The method further involves isolating the olefin from the host cell or from the culture medium. The method further involves cracking or refining the olefin. Preferred Biofuel: The olefin has delta 13C of -15.4 or more, -15.4 to -10.9, or -13.92 to -13.84. The olefin has fM14C of at least 1.003, 1.01, 1.5, 1.111-1.124. EXAMPLE - No suitable example given.