TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: The transgenes are introduced into the organism by glass bead agitation, microparticle bombardment, or electroporation. The feedstock is genetically modified. The method further includes treating the feedstock by heating to 140-200 degrees C, adding dilute sulfuric acid, steam explosion, ammonia fiber explosion, acid or alkaline treatment, or physical disruption. The physical disruption is achieved by chopping, grinding, sonicating, pressing, or exposure to vacuum, adding chemical compounds or enzymes, or exposure to freezing or high temperature. The additive organism is added before or after treatment of feedstock. The additive organism is modified to produce enzyme(s) that results in the lysis of its own cells upon administration of eliciting signal including exposure to specific temperature or chemical elicitor. Preferred Components: The transgenes are present in a construct or minichromosome. The minichromosome further comprises promoter induced by heat or by decrease or increase in pH. Each transgene comprises one of 40 polynucleotide sequences given in the specification, e.g. SEQ ID NOs. 1-10. The additive organism is plant, alga, or fungus, preferably Lemna minor, Chlamydomonas reinhardii, Chlamydomonas, Dunaliella, or Chlorella. The enzyme is from plant, protist, fungi, bacterium, archaea, or animal. The enzymes are cellulose or alpha -glucosidase. The enzyme breaks down glucans and is selected from endo- beta (1,4)-glucanase, cellobiohydrolase, beta -glucosidase, alpha -/ beta -glucosidase, mixed-linked glucanase, endo- beta (1,3)-glucanase, exo- beta (1,3)-glucanase and beta -(1,6)-glucanase. The enzyme breaks down xyloglucan, xylan, or mannan and is selected from hemicellulase/xylanase, endo-1,4- beta -xylanase, beta -xylosidase, glycosyl hydrolase, alpha -l-arabinofuranosidase, alpha -glucuronidase, xyloglucan-specific endoglucanase, oligoxyloglucan reducing end-specific xyloglucanase, alpha -fucosidase, alpha -xylosidase, endo- beta (1,4)-xylanase, beta -xylosidase, beta -xylosidase/ alpha -arabinosidase, acetylxylan esterase, ferulic acid esterase, alpha -glucuronidase, endo- beta (1,4)-mannanase, beta -mannosidase and alpha -galactosidase. The enzyme breaks down cell wall components and is selected from ligninase, acetylesterase, pectinase, pectin lyase, pectate lyase, endo-polygalacturonase, exo-polygalacturonase, pectin methyl esterase, rhamnogalacturonase, rhamnogalacturonan lyase, rhamnogalacturonan acetylesterase, alpha -L-rhamnosidase, endo- alpha (1,5)-arabinosidase, alpha -L-arabinofuranosidase, endo- beta (1,4)-galactanase, xylogalacturonase and beta -galactosidase. The enzyme removes fermentation inhibitor(s) and is nicotinamide adenine dinucleotide phosphate (NADPH)-dependent alcohol dehydrogenase. The enzyme improves fermentation and produces nutrients (preferably vitamin B or lipids) for yeast growth. Preferred Product: The biofuel is ethanol, propanol, butanol, methanol, methane, 2,5-dimethylfuran, dimethyl ether, biodiesel, paraffin, or hydrogen. EXAMPLE - No suitable example given.