TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Polypeptide/Polynucleotide: The variant has at least 80% sequence identity with the sequence of SEQ ID NO: 3. The fragment is at least 50-150 amino acids in length. The polypeptide has cellobiohydrolase activity. The polynucleotide hybridizes under high stringency conditions with a polynucleotide being the reverse complement of SEQ ID NO: 1 and/or SEQ ID NO: 2. Preferred Construct: The GC content is 56% or more, 58% or more, or from 58-65%. Preferred Cell: The cell is a eukaryote cell. The cell is a fungal cell is selected from the genera Aspergillus, Trichoderma, Hypocrea, Fusarium, Disporotrichum, Penicillium, Acremonium, Neurospora, Thermoascus, Myceliophtora, Sporotrichum, Thielavia, Chryosporium, Fusarium, Humicola, Neurospora and Talaromyces or where the fungal cell is of the species Aspergillus oryzae, Aspergillus sojae, Aspergillus nidulans, or a species from the Aspergillus niger group. One or more gene is deleted, knocked-out or disrupted in full or in part, where optionally the gene encodes for a protease. Preparation (claimed): Preparing a polypeptide having carbohydrate material modifying activity comprises cultivating a cell or plant above under conditions which allow for expression of the polypeptide and, optionally, recovering the expressed polypeptide. The cell is a filamentous fungal cell. Preferred Composition: The cellulase is a cellobiohydrolase I, cellobiohydrolase II, an endo- beta -1,4-glucanase, a beta -glucosidase or a beta -(1,3)(1,4)-glucanase. The hemicellulase is an endoxylanase, a beta -xylosidase, an alpha -L-arabinofuranosidase, an alpha -D-glucuronidase, a cellobiohydrolase, an acetyl xylan esterase, a feruloyl esterase, a coumaroyl esterase, an alpha -galactosidase, a beta -galactosidase, a beta -mannanase or a beta -mannosidase. The pectinase is an endo-polygalacturonase, a pectin methyl esterase, an endo-galactanase, a beta galactosidase, a pectin acetyl esterase, an endo-pectin lyase, pectate lyase, alpha rhamnosidase, an exo-galacturonase, an expolygalacturonate lyase, a rhamnogalacturonan hydrolase, a rhamnogalacturonan lyase, a rhamnogalacturonan acetyl esterase, a rhamnogalacturonan galacturonohydrolase, a xylogalacturonase, an alpha-arabinofuranosidase or an endo-arabinanase. The composition comprises a ligninase, an expansin, an expansin-like polypeptide, a swollenin or the polypeptide product of a gene encoding a cellulose integrating protein or a cellulose induced protein. Preferred Method: In the method for treating a substrate comprising non-starch carbohydrate material, optionally a plant material, the substrate is treated to produce sugar from a ligno-cellulosic plant material. SPECIFIC SEQUENCES - The carbohydrate modifying polypeptide comprises the amino acid sequence of SEQ ID NO: 3 encoded by a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1 and/or SEQ ID NO: 2. Sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication.    EXAMPLE - No suitable example given.