NOVELTY - An establishment of genetic transformation method of heavy metal hyper-accumulator Thlaspi caerulescens and culture of transgenic seedlings comprises: (a) establishing primary culture-seedlings of T. caerulescens; (b) culture of cespitose buds; (c) pre-culture of cespitose buds; (d) activation of Agrobacterium tumfaciens; (e) Agrobacterium tumfaciens infection and co-culture of the pre-cultured cespitose buds; (f) front culture; (g) screening culture; (h) seedling culture; (i) rooting culture; and (j) permanent planting of transplantation.    USE - The method is useful for establishing genetic transformation of heavy metal hyper-accumulator Thlaspi caerulescens and culture of transgenic seedlings (claimed).    ADVANTAGE - The method is capable of excluding the effects of the external conditions, and being performed the four seasons, saving the ground for culturing seedlings, lowering the production cost, and obtaining a great quality of strong transgenic seedlings in a short period, and satisfying the research of molecular biology and the production application.    DETAILED DESCRIPTION - An establishment of genetic transformation method of heavy metal hyper-accumulator Thlaspi caerulescens and culture of transgenic seedlings comprises: (a) establishing primary culture-seedlings of Thlaspi caerulescens, taking mature seeds of Thlaspi caerulescens, using alcohol to perform the surface sterilization, and then placing in mercury bichloride and taking out after dipping, washing by sterile water, sterilizing, absorbing the water on the surface by filter paper; (b) culture of cespitose buds of Thlaspi caerulescens, placing the seedlings of Thlaspi caerulescens to a differentiation culture medium to perform the differentiation culture of the cespitose buds for 10-14 days, differentiating the cespitose seedling namely the cespitose buds at the eustipes; (c) pre-culture of cespitose buds of Thlaspi caerulescens, flatly putting the cespitose buds of Thlaspi caerulescens on a culture medium of the pre-culture to pre-culture for 2-3 days; (d) activation of Agrobacterium tumfaciens, respectively picking monocolony of Agrobacterium tumfaciens of an expression carrier of a dicotyledon having an object gene Bjcat5, inoculating to 5 milliliters of LB Agrobacterium tumfaciens liquid culture medium to culture for 8 hours; (e) Agrobacterium tumfaciens infection and co-culture of the pre-cultured cespitose buds of Thlaspi caerulescens, placing the cespitose buds of Agrobacterium tumfaciens pre-cultured for 2-3 days to the inflection liquid culture medium having optical density 600 (OD600) of Thlaspi caerulescens 0.15; (f) front culture, picking out the cespitose buds of Thlaspi caerulescens after the culture in (e), respectively placing in triangular flasks; using sterile water to wash 3-5 times, and using sterile water having 500 milligrams /liter of carbenicillin for 3 times, 10 minutes each time; (g) screening culture, picking out the cespitose buds of Thlaspi caerulescens after the front-culture for 3-15 days, transferring to a screening culture medium; (h) seedling culture, shearing the cespitose buds in the test tubes not less than 3 centimeters after the screening culture into stem sections having 2-3 nodes, inoculating to a seedling culture medium for 18-35 days, containing the seedlings in the test tubes; (i) rooting culture, inoculating the 3.5-8 centimeters of seedlings in the test tubes after the seedling culture to a rooting culture medium to culture for 18-25 days, obtaining the rooted seedlings; and (j) permanent planting of transplant, picking out the aquicultured roots not less than 5 centimeters after the aquiculture culture, transplanting to nursery substrates, performing the permanent planting of transplant; the nursery substrates are composed of soils and vermiculites by the weight proportion 1:1; before transplanting the seedlings, deeply pouring the nursery substrates by Granda nutrient fluid culture medium; after the transplantation, using polyvinyl chloride film to cover, keeping 90-95% of humidity, taking caring of the ventilation; spraying water one time per 5 days, spraying Granda nutrient fluid culture medium one time per 14 days; removing the film after the permanent planting of the transplanted seedlings for 10 days; and keeping 80% of the room humidity, temperature 20-30 degrees C, spraying water three times each day, spraying Granda nutrient fluid culture medium one time per 14 days; spraying urea one time per 21 days, fast culturing, accomplishing the culture of the transgenic seedlings of Thlaspi caerulescens.