NOVELTY - Pure fungus seed of anaerobic degradation polycyclic aromatic hydrocarbon comprises preparing anaerobic agar culture medium with polycyclic aromatic hydrocarbon anaerobic degradation mixed with fungus on the base plate; sealing the culture medium plate, putting it into the drier full of nitrogen; adding colony in the culture medium plate into resazurin anaerobic bottle with polycyclic aromatic hydrocarbon, electron acceptor, basic culture medium, micro-content metal liquid, and vitamin c liquor, rotating; and repeating purifying and substituting fungus.    USE - Pure fungus seed of anaerobic degradation polycyclic aromatic hydrocarbon for biological degradation processing of polluted earth and water.    ADVANTAGE - The method provides good anaerobic degradation to polycyclic aromatic hydrocarbon.    DETAILED DESCRIPTION - INDEPENDENTS CLAIMS are pure fungus seed of anaerobic degradation polycyclic aromatic hydrocarbon comprises (a) adding 99 ml basic culture medium, 1.0 ml micro-content metal liquid, 0.1 ml vitamin C liquor and 0.1 resazurin liquor to anaerobic bottle to make 150-200ml, aerating for 3.5 hours by high pure nitrogen, adding 50-60 mg Na2-S to remove the left oxygen, sealing bottle opening with fluorinated rubber stopper and tightening the screw bottle cap in which the composition of basic culture medium is ammonium chloride (NH4-Cl), potassium dihydrogen phosphate (KH2-PO4), magnesium chloride (MgCl2) and calcium chloride dihydrate (CaCl2.2-H2-O) and composition of micro-content metal liquid is cobalt chloride hexahydrate (CoCl2.6-H2-O), copper chloride (CuCl2), boric acid (H3-BO3), manganese chloride tetrahydrate (MnCl2.4-H2-O), sodium molybdate dihydrate (Na2-Mo4.2-H2-O), nickel chloride dihydrate (NiCl2.2-H2-O) and zinc chloride (ZnCl2); (b) adding 50 ml liquid culture medium, 0.25-0.3 g electron acceptor and 0.65 g agar to anaerobic glove box to make 80-100 ml, sealing the bottle opening with fluorinated rubber stopper and tightening the screw bottle cap, sterilizing in pressure cooker for 20 minutes at 121 degrees C, cooling to 60 degrees C and pouring the culture medium into the culture dish in anaerobic glove box full of nitrogen for 2 days; (c) adding 0.3 ml of 2 g/l polycyclic aromatic hydrocarbon acetone liquor in culture dish, rotating the dish continuously and distributing equally the polycyclic aromatic hydrocarbon acetone liquor and putting the culture dish for 2 days in anaerobic glove box full of nitrogen; (d) adding 10 ml liquid culture and 0.25 g agar in anaerobic bottle to make 20-25 ml, sealing and tightening the cap, sterilizing in pressure cooker for 20 minutes at 121 degrees C and cooling to 60 degrees C; (e) adding 1 ml polycyclic aromatic hydrocarbon anaerobic degradation colony obtained from enrichment and domestication selection, shaking, absorbing 1 ml and adding into culture dish, rotating, sealing and placing in the drier full of nitrogen until colony appears after 1-2 months;(f) adding 2.5-3 ml of 2 g/l polycyclic aromatic hydrocarbon acetone liquor in anaerobic bottle in anaerobic glove box full of nitrogen for 2 days, adding culture medium and 0.05-0.06 g electron acceptor; (g) collecting colony in culture dish, inoculating to anaerobic bottle, sealing, culturing for 20-25 days in agitator at 20\l2 degrees C and rotating at 100 r/minute; and (h) repeating the separation purifying method for greater than or equal to 4 periods, substituting fungus to polycyclic aromatic hydrocarbon anaerobic degradation colony liquor at the last period.    (1) Pseudomonas spp. carries anaerobic degradation to naphthalene with initial concentration of 27.4 mg/l to less than or equal to 0.04 mg/l after 15 days under nitrate reduction;    (2) Pseudomonas spp. carries anaerobic degradation to fragrant with initial concentration of 1.08 mg/l to less than or equal to 0.01 mg/l after 15 days under nitrate reduction;    (3) Pseudomonas spp. carries anaerobic degradation to fluorene with initial concentration of 0.13 mg/l to less than or equal to 0.01 mg/l after 10 days under nitrate reduction;    (4) Pseudomonas spp. carries anaerobic degradation to pyrene with initial concentration of 0.12 mg/l to less than or equal to 0.01 mg/l after 10 days under nitrate reduction.