NOVELTY - Determining oxygen consumption and oxygen consumption rate of oil-hidden microbe comprises: (a) infusing the oil well extraction water or infusion water containing 0.5-5% of nutrient components by weight into a culture container; (b) infusing oxygen or air in the air bottle into the culture container through an air flow control apparatus; (c) cultivating for 5-30 days; (d) after finishing cultivation, analyzing colony density and analyzing colony structure; and (e) taking gas sample to perform gaseous chromatographic analysis.    USE - The method is useful for determining oxygen consumption and oxygen consumption rate of oil-hidden microbe (claimed).    DETAILED DESCRIPTION - Determining oxygen consumption and oxygen consumption rate of oil-hidden microbe comprises: (a) infusing the oil well extraction water or infusion water containing 0.5-5% of nutrient components by weight into a culture container, which is stainless steel and has a volume of 100-500 ml, through a displacement pump; (b) infusing oxygen or air in the air bottle into the culture container through an air flow control apparatus, where the air flow is 1-5 folds of volume of water sample; (c) cultivating for 5-30 days at an analog oil-hidden temperature of 45-80 degrees C and under a pressure of 10-20 MPa; (d) after finishing cultivation, analyzing colony density and analyzing colony structure; and (e) taking gas sample to perform gaseous chromatographic analysis, combining the colony analysis result, calculating oxygen consumption of oxygen; where analysis items of physical and chemical properties of culture solution in (d) comprises: surface tension, content of volatile fatty acid; analysis of all gas components in gas sample in (e) is determined by gaseous chromatogram, the result is represented by volume percentage of each component, the analyzed gas comprises: oxygen, nitrogen, methane, ethane, propane and carbon dioxide; the colony counting in (e) uses bacterial bottle diluting method, the analyzed colony comprises: a hydrocarbon oxidized bacteria, saprophytic bacteria, zymocyte, sulfate reducing bacteria, nitrate reducing bacteria, ferric bacteria and methane production bacteria; the bacteria cultivation is carried out at a temperature of analog oil-hidden environment; the final determination of microbial oxygen consumption and oxygen consumption rate is determined through variable rate of content of oxygen analyzed by the gaseous chromatogram; the gas sample can be taken to analyze chromatogram of gas component quantitatively in any time of culture time, in order to determine microbe metabolic characteristics in different times; the air flow control apparatus in (b) is composed of a Brooks air flow meter and a control instrument.    DESCRIPTION OF DRAWING(S) - The drawing show a flow process for determining oxygen consumption and oxygen consumption rate of oil-hidden microbe (Drawing includes non-English language text).