TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Composition: The biofuel further comprises an ethanol. Preferred Process: The partial transesterification takes place: with the help of a catalyst, where the catalyst is a carrier bound sn-1,3 regiospecific lipase; via several discontinuous production cycles with gradual addition of enzymes; and by stoichiometrical addition of alcohol. The addition of alcohol takes place continuously or in several steps during the transesterification. In the transesterification process, first the alcohol is extensively liberated from the lipase, the reaction mixture containing triglyceride is dissolved; and the reaction mixture is in contact with the catalyst after the complete dissolution of the alcohol with lipase. The alcohol concentration of less than or equal to 5 mass% is used in the reaction mixture to contact with the lipase. The biofuel is mixed with fossil fuel, biodiesel or biomass-to-liquid fuel. Preferred Components: The ratio of mass fraction of the diglyceride to the monoglyceride is greater than or equal to 2. The amounts of the mass fraction of the alkyl esters of fatty acids are minimum of 15% and maximum of 35%. The amounts of the mass fraction of the triglycerides are at least 30% or greater than 40%, preferably maximum of 65%. The mass fraction of the monoglyceride and the diglyceride lies at 0.1-10% and 4-30%, respectively. The mass fraction of the alkyl esters of fatty acids, the triglycerides, the diglyceride and the monoglyceride lies at 20-25%, 50-55%, 20% and 5%, respectively. The mass fraction of ethanol is up to 2%. The alcohol concentration is not exceeded by 3 mass% during the addition of alcohol in several steps. The triglyceride is vegetable oil or vegetable fat. EXAMPLE - Rapeseed oil (100 g) was completely dissolved in ethanol (2 g). The transesterification was started under the addition of an immobilized sn-1,3 regiospecific lipase (1 g). The mixture was mixed for 3 hours at the temperature with the highest lipase activity. After 3 hour, lipase free intermediate (50 ml) was removed from the reaction vessel. The removed reaction medium was completely dissolved in ethanol (2 g). Then, the ethanol solution product was conducted back into the reaction vessel and this was repeated for another 3 hours. After a total of 10 hours, the lipase from the reaction product was separated to give a clear, single-phase liquid comprising alkyl esters of fatty acids (30 mass-%), diglyceride (24 mass%), monoglyceride (9 mass%), triglycerides (36 mass%) and ethanol (1 mass%).