TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Method: The two lysis steps occur in two different reactors (4, 5) connected by a separation unit. A recirculation step comprises conveying the larger dimension organic molecules separated from the vegetal matter in the separation unit to the first reactor. Another separation step occurs in a separation unit (8) placed downstream from the second reactor, where at least part of further larger dimension organic molecules are separated from the vegetal matter. Another recirculation step comprises conveying the further larger dimension organic molecules separated from the vegetal matter in the second separation step to the second reactor. The lysis reactions in the first step are endolysis reactions induced by an enzymatic preparation having amylase, cellulase, protease, pectinase, or xylanase activity. The lysis reactions in the second step are exolysis reactions induced by an enzymatic preparation having glucosidase, aryl esterase, maltase, or cellobiase activity. The lysis reactions occur in the presence of water. The first separation step comprises mechanical filtration of the vegetal matter by filtering through a membrane having a porosity lower than 6 mu m (preferably 110-80 or 11-8 kDa). During the first separation step, a pressure of greater than or equal to 2 bar is applied to the vegetal matter upstream from the membrane. A negative pressure is applied to the vegetal matter through the membrane. The first separation step comprises a micro-filtration step using a membrane having a porosity of 2-5 mu m and subsequently an ultra-filtration step using a membrane having a porosity of 110-80 kDa. The second separation step comprises filtration using a membrane having a porosity lower than 11 (preferably lower than 1.1) kDa. A concentration step following the second concentration step comprises separating a liquid component, particularly water, from a treated vegetal matter by reverse osmosis. A third separation step follows the second separation step where components of the vegetal matter are mutually separated. A fuel generation step follows the second separation step where the vegetal matter is at least partially transformed in fuel through a microbial process. An energy production step follows the second separation step where the vegetal matter is used as fuel. EXAMPLE - Wheat bran was added with deionized water (8 times the amount of treated bran by weight), stirred, added with preservatives (0.6 wt./vol.% m-parahydroxybenzoate, 3 wt./vol.% citric acid, 3 wt./vol.% lactic acid, and/or 1.5 wt./vol.% EDTA). The suspension was thermally treated at 75 degrees C for 75 minutes, added with enzymatic preparation having amylase (1300 KU/L), xylanase (140 KU/L), pectinase (400 KU/L), cellulase (5.2 U/L), and protease (1500 KU/L), and stirred for 11 hours at 50 degrees C. The liquid was cooled down at room temperature and subjected to micro-filtration through a tubular ceramic membrane having a porosity of 3.5 mu m. The concentrate (0.47 L) was sent again to the thermal pre-treatment step and mixed with new suspension (2.21 L). Microfiltered permeate (2.5 L) was subjected to ultrafiltration through a tubular ceramic membrane having a porosity of 100 kDa to yield concentrate (0.32 L) that was sent again to the thermal pre-treatment step and permeate (2.17 L). The permeate underwent an enzymatic hydrolysis treatment with enzymatic preparations having amylase (1300 KU/L), xylanase (160 KU/L), pectinase (400 KU/L), cellulase (4.8 KU/L), protease (1500 KU/L), aryl-esterase (1900 KU/L), and glucosidase (380 U/L) and stirred for 11 hours at 50 degrees C. The hydrolyzed product (2.17 L) was subjected to ultrafiltration to yield concentrate (0.28 L) that was sent again to hydrolysis treatment step and permeate (1.89 L). Permeate was subjected to enzymatic hydrolysis treatment with enzymatic preparations having aryl-esterase (1900 KU/L) and glucosidase (335 U/L) and stirred for 11 hours at 50 degrees C. Hydrolyzed product (1.89 L) was subjected to ultra-filtration to yield concentrate (0.24 L) that was sent again to hydrolysis treatment step with exo-enzymes and ultrafiltration step and permeated matter (1.65 L). The permeated matter was enriched with chemical compounds with a weight lower than 1000 g/mole.