NOVELTY - Silver nanoparticles associated with a biocomponent of the genus Lactobacillus (20-80 wt.%), where the silver nanoparticles having a ratio of the average particle size to the specific surface area (BET) is 0.015-0.15 nm/m2/g.    USE - For manufacturing article or composition having anti-microbial activity (claimed) for inhibiting bacteria e.g. staphylococcus aureus, fungi e.g. candida albicans, and algae e.g. chlorella vulgaris; for surface coating in medical application, and in water treatment filters; and in the formulated biocides, antimicrobials and disinfectants, electronic chemicals, silver conductive ink, medical applications, solar panels and smart glass.    ADVANTAGE - The silver nanoparticles have increased antimicrobial reactivity, good dispersive behavior and a limited size distribution. The silver nanoparticles produced in high concentrations without the need for stabilizing polymers or potentially harmful organic molecules. The method is cost-effective production method. The most of the microorganisms which are contemplated for use in nanoparticles are cheaply and easily produced in large quantities. The bionanoparticles produced within a narrow size range e.g. 3-4 nm on average, and used as a source of controlled Ag+ release, retaining high antimicrobial activity over time, both inside polymers, inside porous resins or other compounds, and in dispersions. The method provides improved conversion of silver salt into silver nanoparticles. The size distribution of the silver nanoparticles is relatively tight and at least partially tuned by differential extraction. The small nanoparticles with good dispersive behavior result in very limited agglomeration, and for the smaller nanoparticles, needed less silver to obtain antimicrobial properties. The larger silver nanoparticles have good antimicrobial effectiveness. The amines and/or thiol-bearing molecules were conjugated to silver nanoparticle that allows long-term storage of silver nanoparticles without appreciable agglomeration. The bionanosilver particles show a high UV absorption maximum at 425 nm, even at low concentrations of 40 mg/liter Ag. The bionanosilver particles show excellent biocidal properties against algae, Gram positive bacteria, Gram negative bacteria, fungi.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for following:    (1) method (P1) of producing silver nanoparticles involving incubating bacteria of the genus Lactobacillus with an aqueous solution containing a silver salt (at least 4 mM) in the presence of ammonia and an alkali metal hydroxide until a biomass of bacteria containing Ag(0) silver nanoparticles is formed;    (2) an anti-microbial composition (C1) comprising the silver nanoparticles; and    (3) manufacturing an article or a composition with anti-microbial activity involving dispersing or impregnating silver nanoparticles into the article or composition.