NOVELTY - Synthesis of biodiesel from oil involves preparation of lipase by preparing crude lipase liquid, culturing Yarrowia lipolytica LW1 strain in eggplant-shaped flask, inoculating to seed fermentation cylinder, and culturing; preparing immobilized lipase; performing oil hydrolysis by adding oil in reactor; hydrolyzing; and performing esterification.    USE - Method for synthesizing biodiesel from oil (claimed).    ADVANTAGE - The hydrolysis and esterification of oil are catalyzed by lipase, not strong acid or base, thus the reaction condition is mild and pollution is small. The production of methyl/ethyl fatty acid ester does not use organic solvent, thus it is safe and efficient.    DETAILED DESCRIPTION - Synthesis of biodiesel from oil involves preparation of lipase by preparing crude lipase liquid, culturing Yarrowia lipolytica LW1 strain on slant in eggplant-shaped flask, inoculating to seed fermentation cylinder, culturing for 80-100 hours, and centrifuging fermentation liquor when hydrolase activity is greater than or equal to 8000 mu m/ml to remove solid and fungi, which form crude lipase liquid. Immobilized lipase is prepared by adding immobilizing auxiliary (0.1-0.5 wt.%) in the crude lipase liquid, mixing carrier and the liquid at volume ratio of 1:1-1.5, making enzyme on carrier (1 g) 8000-13000 Unit, drying at 50 degrees C, and making water content of carrier to 4-8 wt.%. Hydrolysis of oil involves adding oil in reactor; adding the crude lipase liquid (50-100 mu /g) in the reactor; hydrolyzing for 12-36 hours in condition that weight ratio of oil to water is 1:0.2-1:1.2, in which dodecyl sodium sulfate or sodium stearate (0.05-0.2 g) is used as emulsifier for 1 g of oil, pH of water phase is 5.5-7.0, temperature is 20-55 degrees C and vibration speed is 100-300 rpm; when hydrolysis rate is larger than 90%, acidifying the reactant by 2-4 N acid; making pH of water phase 3.5-5.5; washing using 60-80 degrees C water; placing the reactant to form water phase with 6.1-8.3 wt.% of glycerin and crude fatty acid with a little of water; dewatering the crude fatty acid to form fatty acid for esterifying; and purifying the water phase to form highly pure industrial glycerin or medical glycerin. Esterification involves mixing the crude fatty acid with methanol or ethanol at mol ratio of 1:1-1:1.5; adding the immobilized lipase; esterifying at 30 degrees C during vibrating for 3-24 hours to form methyl or ethyl fatty acid ester, where conversion of the ester is 90% measured by gas phase chromatography; treating the ester by vacuum distillation under 93-98 KPa; and collecting fraction at 190-220 degrees C. An INDEPENDENT CLAIM is included for biodiesel comprising oleic acid which is ester of 16-18C fatty acid and methanol or ethanol, and includes linoleic acid, stearic acid, and hexadecanoic acid.