NOVELTY - Increasing utilization rate of perennial pasture and its weed comprises initially selecting lignin degrading bacteria and cellulose degrading bacteria capable of growing in mixture on potato dextrose agar (PDA) solid culture medium, and culturing inclined culture; inoculating cultures capable of being mixed together on solid fermentation culture medium and sieving mixture culture capable of cooperating in solid fermentation; comparing types of degrading enzymes; and expanding and culturing sieved optimized mixed culture to prepare spore and hyphae.    USE - Increasing utilization rate of perennial pasture and its weed for producing green reproducible biofuel ethanol with lignocellulose substance as raw material (claimed).    ADVANTAGE - The method has simple and easy operation; can increase degradation rate of lignin by 46.25%, degradation rate of cellulose by 10.09%, and degradation rate of hemicellulose by 11%; and greatly reduces production cost and increases utilization and conversion rate of perennial pasture.    DETAILED DESCRIPTION - Increasing utilization rate of perennial pasture and its weed comprises:    (A) initially selecting lignin degrading bacteria and cellulose degrading bacteria capable of growing in mixture on PDA solid culture medium after culture tests, culturing inclined culture for 7 days using PDA culture medium as base culture medium, and observing growth rate, growth appearance and interaction between cultures on PDA culture medium;    (B) inoculating cultures capable of being mixed together on solid fermentation culture medium based on certain inoculating ratio, where solid fermentation measures lignin degradation enzyme activity, cellulose degradation enzyme activity and content change of three elements in natural pasture, and sieving mixture culture capable of cooperating with solid fermentation;    (C) comparing types of degrading enzymes produced by single culture and degradation enzyme activity with that of mixed culture, preferably choosing mixed optimized organization with stronger degradation enzyme activity and higher degradation rate of perennial lignocellulose substance, and further observing growth; and    (D) expanding and culturing sieved optimized mixed culture into production culture for further expansion of thalli quantity and synthesis of product to prepare spore and hyphae.