NOVELTY - A nanolipoprotein particle comprises a target protein, a membrane forming lipid, and a scaffold protein, where the target protein is a membrane associated hydrogenase.    USE - The nanolipoprotein particle is useful in biocatalyst assembly for performing a chemical reaction catalyzed by a membrane associated hydrogenase (claimed), and is applied in various fields such as basic biology research, applied biology, bioengineering, bioenergy and biofuels.    ADVANTAGE - The method enables to solubilize a membrane associated hydrogenase enzyme with excellent unique functionalities, such as oxygen stability, preferential hydrogen producing activity and/or thermal stability.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following:    (1) biocatalyst assembly comprising the nanolipoprotein particle, where the nanoolipoprotein is immobilized to a support;    (2) method for performing a chemical reaction catalyzed by a membrane associated hydrogenase, involves providing reagents for performing the chemical reaction, providing a nanolipoprotein particle comprising the membrane associated hydrogenase, a membrane forming lipid and a scaffold protein, and contacting the reagents with the nanoliproprotein particle for time and under conditions to allow the reaction to occur, thus performing the chemical reaction;    (3) system for performing a chemical reaction catalyzed by a membrane associated hydrogenase, comprises the nanolipoprotein particle, where the membrane associated hydrogenase is the membrane associated hydrogenase catalyzing the chemical reaction, and reagents for performing the chemical reaction, where the nanolipoprotein particle and the reagents can be contacted for a time and under condition to allow the chemical reaction catalyzed by the membrane associated hydrogenase to occur; and    (4) device for performing a chemical reaction catalyzed by a membrane associated hydrogenase, has a substrate compartment, a hydrogenase compartment, and a product compartment, with the substrate compartment in communication with the product compartment through the hydrogenase compartment, where the hydrogenase compartment comprises the biocatalyst assembly, and the substrate compartment is configured to comprise a substrate for the chemical reaction catalyzed by the membrane associated hydrogenase and to allow contact between the substrate and the membrane associated hydrogenase.