TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: Preparing controlled-release biological organic fertilizer of golf course lawn by ternary continuous fermentation, where the first fermentation process is as follows: adding Bacillus, lactic acid bacteria and coccus which have strong degradability and odor-emitting ability to the cushion material in the farm pig room to ferment the pig manure and urea; the secondary fermentation is as follows: stacking and fermenting the pig manure and cushion material on the basis of the first fermentation, adding fermentation liquid of the lactic acid bacteria which has strong degradability; the third fermentation is as follows: adding fermentation liquid of silicate bacteria to the fermentation product on the basis of the secondary fermentation, obtaining controlled-release biological organic fertilizer particle of the high-grade golf course lawn, where the viable count of the silicate bacteria is 10 to the power of 12/g; the organic substance is more than 30%; the Escherichia coli is less than 5/g; the roundworm death rate is more than 98%; the effective microorganism is 10 to the power of 13/g; the specific step is as follows: (a) manufacturing fermentation bed: (i) activating EM strain which is purchased from market: using 10g of EM strain, 0.1kg of brown sugar (the brown sugar is molten by hot water) and 1kg of water to carry out culturing at 37 degrees C; and (ii) manufacturing the fermentation bed: adding EM strain liquid to the pig room cushion material according to 10% of inoculating amount; using the stirrer to fully stir them, adding the mixture to the fermentation bed; after reacting for 48 hours at 25 degrees C, breeding pig; (b) naturally fermenting: uniformly mixing the manure and urea and cushion material by self movement of the pig and turning of worker at 25 degrees C, where the natural fermentation time is 1-2 years; the natural fermentation has strong and effective deodorizing and sterilizing; (c) preparing lactic acid bacteria fermentation agent: (i) preparing a first expanding culture medium: the formulation of the culture medium is as follows: 50g of soybean peptone, 30g of beef powder, 30g of yeast powder, 200g of glucose, 200g of lactose, 50ml of distilled tomato juice and 1000ml of water; using high-pressure steam whose temperature is 121 degrees C to sterilize them for 20 minutes, for use; (ii) first expanding culture: loading the culture medium to 2000ml of triangle flask; loading 5g of lactic acid bacteria to the triangle flask, where the lactic acid bacteria has 10 to the power of 9/g of viable count and contains plant milk, Bacillus, Lactobacillus delbrueckii, Lactococcus lactis, Bifidobacterium and active diastase; carrying out anaerobic static culturing for 16-18 hours at 36|\1 degrees C; when the pH of the fermentation liquid arrives at 3.8-4.5, it is shown that the fermentation state is good; (iii) preparing a secondary expanding culture medium: 1000g of soybean peptone, 100g of cottonseed sugar, 500ml of tomato juice, 50g of sodium chloride and 10000ml of water, using high-pressure steam whose temperature is 121 degrees C to sterilize them for 20 minutes, for use; and (iv) secondary expanding culture: adding the culture liquid to the fermentation tank; inoculating the fermentation liquid of the first fermentation to the culture liquid of the second culture; carrying out anaerobic static culturing for 12-14 hours at 40 degrees C; when the pH of the fermentation liquid arrives at 3.8-4.5, it is shown that the fermentation state is good; (d) lactic acid bacteria anaerobic fermenting: inoculating the lactic acid bacteria fermentation preparation to the fermentation substance obtained by naturally fermenting, where the inoculating amount is 2% of the secondary fermentation amount; mixing them uniformly; using soil shifter to push the mixed fermentation substance needing to secondarily ferment to the sealed fermentation cellar built by bricks and cement, maintaining the temperature at 25 degrees C, carrying out anaerobic fermenting for 36 hours to kill the Escherichia coli and roundworm in the pig manure, improve trace element content of the biological organic fertilizer, convert the organic macromolecule to the small molecule for the plant absorption and effectively regulate acid-alkali degree of the organic fertilizer; (e) preparing silicate bacteria fermentation liquid: (i) preparing the first silicate bacteria culture medium: the formulation of the culture medium is as follows: 5g of sucrose, 2g of sodium dihydrogen phosphate (Na2HPO4), 0.5g of magnesium sulfate heptahydrate (MgSO4.7H2O), 0.005g of iron chloride (FeCl3), 0.1g of calcium carbonate (CaCO3), 0.5g of bauxite, 1000ml of water, at pH 7.0-7.5, using high-pressure steam whose temperature is 121 degrees C to sterilize them for 20 minutes, for use; (ii) carrying out first expanding culture of the silicate bacteria: loading 10ml of first culture medium to the 100ml of triangle flask; selecting silicate bacteria in every culture flask, namely, selecting big Bacillus and gelatin Bacillus strain, selecting one single colony on the inclined plane of the big Bacillus and gelatin Bacillus strain obtained by ultraviolet ray inducing and separating the stain purchased China Centre for Type Culture Collection, which has strong phosphorus-producing and potassium-producing ability, culturing the strain for 24 hours at 37 degrees C in the shaking bed; (iii) preparing the second silicate bacteria expanding culture medium: the formulation of the culture medium is as follows: 0.5% of maize, 0.5% of fish powder, 3.5% of soybean cake, 95.5% of de-ionized water, using high-pressure steam whose temperature is 121 degrees C to sterilize them for 20 minutes, for use; (iv) carrying out secondary expanding culture of the silicate bacteria: loading the secondary culture medium to the fermentation tank, culturing the strain for 24 hours at 37 degrees C on the shaking bed (200 revolutions per minute); and (v) inoculating the silicate bacteria fermentation liquid to the secondary fermentation substance according to 2% of inoculating amount, mixing them uniformly, for use; and (f) preparing biological organic fertilizer: taking glutinous rice soup as regulator for regulating humidity; uniformly mixing the fermentation product of the silicate bacteria and glutinous rice soup till that the humidity is about 10% in order to dry the product; using the pelleting machine to carry out pelleting, drying the product at 45 degrees C, obtaining the product. ACTIVITY - Fertilizer.