TECHNOLOGY FOCUS - BIOLOGY - Preferred Components: The two different enzyme activities include exoglucanase, endoglucanase, hemicellulase, beta -glucosidase (all preferred), feruloyl esterase, xylanase, alpha -L-arabinofuranosidase, endogalactanase, acetylxylan esterase, beta -xylosidase, xyloglucanase, glucuronoyl esterase, endo-1,5- alpha -L-arabinosidase, pectin methylesterase, endopolygalacturonase, exopolygalacturonase, pectin lyase, pectate lyase, rhamnogalacturonan lyase, pectin acetylesterase, alpha -L-rhamnosidase, mannanase, cellulase, licheninase, laminarinase, and/or beta-(1,3)-(1,4)-glucanase. The promoter sequence is a sequence of a promoter comprising a constitutive promoter (preferred) including act1 promoter or 35S cytomegalovirus (CMV) promoter, a developmentally-specific promoter, a tissue-specific promoter, or an inducible promoter. The lignocellulolytic enzyme polypeptide is expressed in at least one targeted sub-cellular compartment or organelle comprising apoplast, chloroplast, vacuole, cell wall, and/or endoplasmic reticulum. The lignocellulolytic enzyme polypeptide is expressed in a plant part comprising stems, leaves, grain, and/or cobs, where the plant includes corn, switchgrass, Sorghum (all preferred), Miscanthus, sugarcane, poplar, pine, wheat, rice, soy, cotton, barley, turf grass, tobacco, bamboo, rape, sugar beet, sunflower, willow, or Eucalyptus. The lignocellulolytic enzyme polypeptide includes endoglucanase, xylanase, (both preferred), cellulase, hemicellulase, ligninase, cellobiohydrolase, beta -D-glucosidase, beta -D-glucan gluchydrolase, xyloglucanse, beta -xylosidase, arabinofuranosidase, acetyl xylan esterase, glucuronidase, rhamnogalacturonase, polygalacturonase, pectin methyl esterase, mannanase, galactanase, arabinase, lignin peroxidase, manganese-dependent peroxidase, hybrid peroxidase, laccase, ferulic acid esterase, glucuronyl esterase, and/or isomerase, where the endoglucanase (E1) comprises endo-1,4- beta -glucanase comprising a fully defined amino acid sequence containing 359 amino acids of SEQ ID NO: 1 as given in the specification, and the xylanase comprises Xyn-Z comprising a fully defined amino acid sequence containing 837 amino acids of SEQ ID NO: 5 as given in the specification.    TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: In the method, the step of tempering comprises a process including ensilement, grinding, pelleting, microwaving, sonication, incubation at a specific temperature, and/or incubation at a specific pH. The step of tempering comprises: incubating the sample at 25-100 degrees C for at least 1 hour, preferably 85 degrees C for at least 24 hours; and ensiling the sample of plant biomass, where the sample is ensiled for at least 5, preferably 20 days, at 37 degrees C or 85 degrees C, and the sample of plant biomass is in solid form or in a liquid slurry during the step of tempering. The step of treating comprises: externally applying an amount of at least one lignocellulolytic enzyme polypeptide, where the amount of externally applied lignocellulolytic enzyme polypeptide required to achieve a given level of hydrolysis is less than the amount of externally applied lignocellulolytic enzyme polypeptide required to achieve the same level of hydrolysis of comparable lignocellulosic biomass from a plant part that is not obtained from a transgenic plant, and the externally applied lignocellulolytic enzyme polypeptide has at least two different enzyme activities; or externally applying an amount of at least two lignocellulolytic enzyme polypeptides, where the two lignocellulolytic enzyme polypeptides together have at least two different enzyme activities. In the method, the gene encoding the lignocellulolytic enzyme polypeptide is fused to a signal peptide sequence, where the signal peptide sequence encodes a secretion signal that allows localization to a cell compartment or organelle comprising cytosol, vacuole, nucleus, endoplasmic reticulum, cell wall, mitochondria, apoplast, peroxisomes, or plastid. The step of treatment further comprises adding a crude extract from the sample of plant biomass, where the crude extract comprises at least one lignocellulolytic enzyme polypeptide encoded by the recombinant polynucleotide. Preferred Components: The externally applied lignocellulolytic enzyme polypeptide is capable of hydrolyzing cellulose to glucose monomers, where a greater level of hydrolysis is obtained from the sample of plant biomass obtained from transgenic plant than from a sample of plant biomass from a non-transgenic plant that is processed under the same conditions. The signal peptide sequence encodes a secretion signal from equistatin of sea anemone, where the secretion signal comprises a KDEL motif (Lys-Asp-Glu-Leu). The crude extract comprises at least one lignocellulolytic enzyme fused to an affinity tag comprising a tag including HAT (histidine affinity tag), FLAG (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C), c-myc (myelocytomatosis viral oncogene homolog) (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu), hemagglutinin antigen, or His (hexahistidine). The sample of plant biomass comprises insoluble fiber, and is obtained from the grain of the transgenic plant. EXAMPLE - No suitable example given.