NOVELTY - Separating propionic acid oxidized hydrogen-producing acetogenic bacteria, involves domesticating anaerobic activated sludge, introducing nitrogen gas into sterile bottle, adding domesticated anaerobic activated sludge, vibrating sludge to obtain suspending solution, enriching suspending solution, preparing colony of propionic acid oxidized hydrogen-producing acetogen and methanogen, preparing propionic acid oxidized hydrogen-producing acetogen colony, and culturing propionic acid oxidized hydrogen-producing acetogen colony in propionic acid culture medium.    USE - The method is useful for separating propionic acid oxidized hydrogen-producing acetogenic bacteria, where the bacteria can degrade high concentrated propionic acid and treats high concentrated organic wastewater.    ADVANTAGE - The method separates the bacteria by a simple process, and the hydrogen-producing rate of the bacterial colony is 6-8 times of the hydrogen-producing rate of the normal acetogen.    DETAILED DESCRIPTION - Separating propionic acid oxidized hydrogen-producing acetogenic bacteria, involves (a) obtaining soybean protein wastewater with chemical oxygen demand (COD) concentration of 2000-10000 mg/l as inlet water, obtaining four chamber anaerobic baffle plate reactor as model and domesticating anaerobic activated sludge such that pH of inlet water is 7-7.25 and temperature is 35 degrees C and hydraulic retention time is 48 hours, (b) introducing nitrogen gas into 120 ml of sterile bottle filled with multiple glass beads, adding 30-60 ml of domesticated anaerobic activated sludge in the third chamber anaerobic baffle plate reactor, and vibrating the sludge for 1-2 hours at 35 degrees C at a speed of 130 rotations/minute to obtain suspending solution A, (c) transferring suspending solution A (10-20 ml) with sterile injector into sterile bottle filled with propionic acid culture medium (10000 mg/l), and enriching the medium at 35 degrees C at 130 rotations/minute at pH 7.0-7.5 to obtain enriched suspending solution, (d) transferring the enriched suspending solution into sterile bottle filled with propionic acid culture medium (10000 mg/l) under the condition that the mass concentration of propionic acid in the culture medium is less than 1000 mg/l, and enriching and culturing the solution at 35 degrees C at a speed of 130 rotations/minute at pH 7-7.5, (e) repeating the step (d) for 8-10 times to obtain colony of propionic acid oxidized hydrogen-producing acetogen and methanogen, (f) transferring propionic acid oxidized hydrogen-producing acetogen and methanogen into propionic acid culture medium containing 2-bromoethyl sodium sulfonate (0.1-10 mol/l), and culturing the colony at 35 degrees C and at pH 7-7.5 at a speed of 130 rotations/minute to obtain propionic acid oxidized hydrogen-producing acetogen colony, (g) transferring propionic acid oxidized hydrogen-producing acetogen colony into propionic acid culture medium containing 2-bromoethyl sodium sulfonate (0.1-10 mol/l) under the condition that mass concentration of propionic acid culture medium containing 0.1-10 mM/l of 2-bromoethyl sodium sulfonate in enrichment culture, and culturing the bacteria at 35 degrees C at a speed of 130 rotations/minute at pH 7-7.5 and (h) repeating the step (g) for 4-8 times and separating propionic acid oxidized hydrogen-producing acetogenic bacteria, where the volume ratio of suspending solution A to propionic acid culture medium in step (b) is 1:6, volume ratio of enriched suspending solution to propionic acid culture medium in step (d) is 1:6, volume ratio of colony of propionic acid oxidized hydrogen-producing acetogen and methanogen to propionic acid culture medium is 1:6, and volume ratio of propionic acid oxidized hydrogen-producing acetogen colony to propionic acid culture medium containing 0.1-10 mol/L of 2-bromoethyl sodium sulfonate is 1:6.