TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation (Claimed): The recombinant yeast cell is prepared by providing recombinant yeast cell comprising butanol biosynthetic pathway, and engineering the yeast cell to comprise at least one genetic modification that increases activity of the high osmolarity/glycerol response pathway. Preferred Method: The genetic modification increases activity of mitogen-activated protein kinase module of the high osmolarity/glycerol response pathway. The genetic modification increases PBS2 mitogen-activated protein kinase activity. The modification increasing PBS2 mitogen-activated protein kinase kinase activity is overexpression of pBS2 protein encoding gene. Preferred Nucleic acid: The 1-butanol biosynthetic pathway comprises at least one gene encoding a polypeptide that performs at least one substrate to product conversion chosen from acetyl-coenzyme A (CoA) to acetoacetyl-CoA (catalyzed by acetyl-CoA acetyltransferase), acetoacetyl-CoA to 3-hydroxybutyryl-CoA (catalyzed by 3-hydroxybutyryl-CoA dehydrogenase), 3-hydroxybutyryl-CoA to crotonyl-CoA (catalyzed by crotonase), crotonyl-CoA to butyryl-CoA (catalyzed by butyryl-CoA dehydrogenase), butyryl-CoA to butyraldehyde (catalyzed by butyraldehyde dehydrogenase) and butyraldehyde to 1-butanol (catalyzed by 1-butanol dehydrogenase). The 2-butanol biosynthetic pathway comprises at least one gene encoding a polypeptide that performs at least one substrate to product conversion chosen from pyruvate to alpha -acetolactate (catalyzed by acetolactate synthase), alpha -acetolactate to acetoin (catalyzed by acetolactate decarboxylase), acetoin to 2,3-butanediol (catalyzed by butanediol dehydrogenase), 2,3-butanediol to 2-butanone (catalyzed by butanediol dehydratase) and 2-butanone to 2-butanol (catalyzed by 2-butanol dehydrogenase). The isobutanol biosynthetic pathway comprises at least one gene encoding a polypeptide that performs at least one substrate to product conversion chosen from pyruvate to acetolactate (catalyzed by acetolactate synthase), acetolactate to 2,3-dihydroxy-isovalerate (catalyzed by acetohydroxy acid isomeroreductase), 2,3-dihydroxy-isovalerate to alpha -ketoisovalerate (catalyzed by acetohydroxy acid dehydratase or dihydroxy acid dehydratase), alpha -ketoisovalerate to isobutyraldehyde (catalyzed by branched-chain keto acid decarboxylase) and isobutyraldehyde to isobutanol (catalyzed by branched-chain alcohol dehydrogenase). The butanol biosynthetic pathway comprises at least one gene that is heterologous to the yeast cell. EXAMPLE - No suitable example given.