NOVELTY - Recombinant nucleic acid encoding a promoter that is upregulated at least 3-fold in a cell of Prototheca when the extracellular environment changes from containing at least 5 mM nitrogen to containing no nitrogen, is new.    USE - The recombinant nucleic acid is useful for encoding a promoter and producing a triglyceride composition, triglyceride oil, a chemical and oil (all claimed), which are useful for producing a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein, which are useful in manufacturing transportation fuels e.g. renewable diesel, biodiesel, and renewable jet fuel.    ADVANTAGE - The cultivation of microorganisms is low cost. The recombinant nucleic acid provides oil in microalgae with shorter chain length and a higher degree of saturation and without pigments, with greater yield and efficiency.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (a1) a nucleic acid vector comprising an expression cassette containing: a promoter that is active in a cell of Prototheca; and a coding sequence in operable linkage with the promoter, where the coding sequence contains the most or second most preferred codons of table 1 of the specification for at least 20% of the codons of the coding sequence; (a2) producing a triglyceride composition, comprising: culturing a population of cells of Prototheca in the presence of a fixed carbon source, where the cells contain an exogenous gene, the cells accumulate at least 10% of their dry cell weight as lipid and the fixed carbon source is Sorghum or depolymerized cellulosic material, and isolating lipid components from the cultured microorganisms; (a3) producing a triglyceride composition, comprising: culturing a population of microorganisms in the presence of depolymerized cellulosic material, where the depolymerized cellulosic material is subjected to washing with water prior to the culturing step, the cells accumulate at least 10% of their dry cell weight as lipid, the depolymerized cellulosic material is concentrated to at least 300 g/l of glucose prior to the cultivation step and the microorganisms are cultured in a fed-batch reaction in which depolymerized cellulosic material of at least 300 g/l of glucose is fed to the microorganisms, and isolating lipid components from the cultured microorganisms; (a4) manufacturing triglyceride oil comprising cultivating a cell that has a 23S rRNA sequence with at least 96% nucleotide identity to SEQ ID NO: 30 (Sequence not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication) in the presence of sucrose as a carbon source; (a5) manufacturing a chemical comprising performing at least one chemical reaction consisting of transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis or saponification on a triglyceride oil; (a6) manufacturing an oil, comprising either hydroxylation reaction, performing an esterification reaction, a hydroprocessing reaction or a deoxygenation reaction consisting of a hydrogenolysis reaction, hydrogenation, a consecutive hydrogenation-hydrogenolysis reaction, a consecutive hydrogenolysis-hydrogenation reaction or a combined hydrogenation-hydrogenolysis reaction; (a7) a recombinant nucleic acid with at least 95% nucleotide identity to SEQ ID NO: 134 and/or 135 (Sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication); and (a8) a recombinant nucleic acid encoding a protein with at least 90% amino acid identity to SEQ ID NO: 136 and/or 137 (Sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication).