NOVELTY - Production of bio-fuel comprises providing feed stock into a pre-fermentation system, which comprises mixing tank, nutrient source, and antibiotic source; directing amended feed stock from the pre-fermentation system to a fermentation system, which comprises first bio-reactor, second bio-reactor, and oxygen source for providing oxygen to the first bio-reactor; generating effluent from the fermentation system; and distilling the effluent from the fermentation system.    USE - The method is for producing bio-fuel (claimed) including butanol, methanol, biodiesel, and preferably ethanol. It is useful for producing food and medical grade materials produced through fermentation, including beer, wine, cheese, and yogurt.    ADVANTAGE - The method does not utilize batch type production, but follows a continuous flow protocol that eliminates much downtime inherent in conventional bio-fuel production systems, while greatly reducing space and equipment requirements. Production is enhanced via controlled program of aeration for microbial growth and anaerobic conditions to ensure fermentation efficiency. As the system becomes more tolerant of alcohol content, efficiency increases. By promoting in-situ growth of yeast and/or other beneficial microbial populations, the method promotes microbial growth and acclimation within the fermentation tanks, piping, and associated components. Over time, the microbial growth can result in the spread of yeast and/or other beneficial microbial agents throughout the fermentation system, improving the fermentation process and overall system efficiency. The method eliminates the need for prolonged storage or retention time common with conventional batch-type production methods, and eliminates the need for large storage tanks common with conventional production methods. It allows for total control of material flow, nutrient addition, and oxygenation using a specific aeration protocol for timed anaerobiosis, allowing for increased and consistent alternative fuels production from feed stocks.