TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: In the method above, the triglyceride is admixed with a lipase and incubated for time and temperature to form the fatty acid. The triglyceride is a member selected from a vegetable oil, an animal fat, tallow, a grease, and a recycled rendered oil or fat. The fatty acid is a C12 to C22 fatty acid. The lipase is derived from a member selected from a plant, a bacteria, a fungi and a higher eukaryotes, where the lipase is derived extracellularly, intracellularly or in the form of a whole-cell. The lipase is immobilized, here the immobilization is on a porous support or a powder, where the porous support is an acrylic resin, and where the lipase is Candida Antarctica lipase B. The lipase is from Candida rugosa. The enzymatic hydrolysis reaction is incubated at 20-50 degrees C. The enzymatic hydrolysis reaction is incubated for 0.5-24 hours. The enzymatic hydrolysis reaction is carried out in the presence of water. The enzymatic hydrolysis reaction is carried out in a buffered solution, where the water to triglyceride ratio is 1:10 to 20:1 (v/v). The lipase concentration is 0.125-2 mg/ml. The lipase can be recycled using a hollow fiber membrane or other filters. The enzymatic hydrolysis reaction further comprises an organic solvent, where the organic solvent is a member selected from a C5-C12 alkane and a sterically hindered alkanol. The esterifying step is chemically or enzymatically catalyzed, where the chemical esterification of the fatty acid comprises admixing the fatty acid with the alcohol and an acid catalyst; and incubating the admixture for a tithe and temperature to form biodiesel. The alcohol is a C1-C5 alcohol. The acid catalyst is selected from an inorganic acid catalyst or an organic acid catalyst, where the acid catalyst is a liquid or a solid. The acid catalyst is an acidic styrene-divinylbenzene sulfonated ion exchange resin. The acid catalyst is regenerated by washing with a polar solvent, where the polar solvent is a member selected from C1-C6 alkanol and a sterically hindered alkanol. The esterifying step comprises an organic solvent, where the organic solvent is a member selected from a C5-C12 alkane and a sterically hindered alkanol. The esterifying step comprises molecular sieves, where the incubation temperature is 20-90 degrees C, where the incubation time is 0.2-24 hours, and where the alcohol to fatty acid ratio is 1:1 to 6:1 (mol/mol). Solid acid catalyst to fatty acid ratio is 1:10 to 10:1 (w/w). The enzymatic esterification of the fatty acid comprises: admixing the fatty acid with the alcohol and a lipase; and incubating the admixture for a time and temperature to form the biodiesel. The lipase is derived from a member selected from a plant, a bacteria, a fungus and a higher eukaryotes. The lipase is derived extracellularly, intracellularly or in the form of a whole-cell. The lipase is immobilized, where the immobilization is on a porous support or a powder, and where the porous support is an acrylic resin. The lipase is C. Antarctica lipase B. The lipase is regenerated by washing with a polar solvent, where the polar solvent is a member selected from a C1-C6 alkanol and a sterically hindered alkanol. The esterification reaction comprises an organic solvent, where the organic solvent is a member selected from a C5-C12 alkane and a sterically hindered alkanol. The esterification reaction comprises molecular sieves, where the incubation temperature is 20-70 degrees C. The incubation time is 0.2-24 hours. The alcohol to the fatty acid ratio is 1:1 to 2:1 (mol/mol). The lipase to the fatty acid ratio is 0.005:1 to 1:1 (w/w). The hydrolysis or esterification is conducted in a reactor, where the reactor is a member selected from a fixed bed reactor, a fluidized reactor and a stirred-tank reactor. The biodiesel is admixed with petrodiesel. EXAMPLE - No suitable example given.