NOVELTY - Fractionating biomass comprises: either permeating walls of biomass cells, liberating cell products from the cells, and fractionating and recovering the liberated cell products and derivatives; or (a) permeability conditioning biomass suspended in a pH adjusted solution of at least one water-based polar solvent to form a conditioned biomass; (b) intimately contacting the biomass with at least one non-polar solvent, partitioning to obtain a non-polar solvent solution and a polar biomass solution; and (c) recovering cell products from the nonpolar solvent solution and polar biomass solution.    USE - The concentrated supplemented media solution is useful for biological growth or fermentation production systems. The method is useful for fractionating biomass to provide biocrude including terpenoids such as sterols and carotenoids, chlorophyll, phospholipids, glycolipids, sphingolipids, triacylglycerols, diacylglycerols, monoacylglycerols, fatty acids, decarboxylated fatty acid hydrocarbon chains, methyl, ethyl, propyl, butyl and/or amyl esters of the fatty acids, aromatics, alkyl aromatics, polyaromatics, naphthalene, alkyl substituted naphthalene, linear and branched alkanes, linear and branched alkenes, alcohols such as methanol, ethanol, butanol or other lipid compounds. The method is useful for fractionating algae biomass to generate end products (all claimed), where the end products are useful for deriving products such as biofuels and other valuable end products. The biomass metabolites containing lipids, proteins and carbohydrates, are useful: as a feedstock for biological production systems, plastic additives (glycols from biomass sugars); in animal nutrition as a feed; and in other fuel producing alternatives (syngas production, methane production by anaerobic digestion, ethanol production via fermentation).    ADVANTAGE - The method is efficient and flexible for deriving large scale end products from algae biomass. The method allows not only targeted product isolation and preconditioning the products, but effective separation of by-products as classes and specific products and recycling nutrients and water.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for:    (1) products recovered from the method, in the non-polar solvent solution comprising lipid products, hydrocarbon chains or organic compounds; or in the polar biomass solution comprising soluble monosaccharides, disaccharides, oligosaccharides, polysaccharides, glycerols, amino acids, soluble proteins, peptides, fiber, nutrients, phosphocholine, phosphate, growth media, or residual solid algae cell structural particles;    (2) a method-1 of operating a renewable and integrated sustainable processing plant for growing and processing high moisture algae, comprising growing biomass algae, harvesting the algae, step (a) and liberating cell products from within the algae, step (b), obtaining growth media from the polar biomass solution and carbon dioxide from the polar biomass solution, and recycling the carbon dioxide and growth media to the algae cultivation system for renewable and sustainable operation;    (3) a method of fractionating biomass comprising steps (a) and (b), and obtaining biocrude; and    (4) the biocrude obtained by the method.    DESCRIPTION OF DRAWING(S) - The figure shows a flow diagram for the large scale method of fractionating biomass.