NOVELTY - Method of producing microbial flocculating agent using culture wastewater, involves obtaining active sludge of sewage treatment plant, screening single colony with excellent growth, preparing seed culture medium, inoculating strain into seed culture medium, fermenting, adding glucose and ammonium sulfate to culture wastewater to obtain culture wastewater fermentation culture medium, sterilizing, inoculating seed liquid to culture effluent fermentation culture medium, culturing, and collecting supernatant containing microbial flocculating agent product.    USE - The method is useful for producing microbial flocculating agent using culture wastewater (claimed).    ADVANTAGE - The method enables to produce microbial flocculating agent simply and cost-effectively, thus can be produced easily in large quantities in industries.    DETAILED DESCRIPTION - Method of producing microbial flocculating agent using culture wastewater, involves obtaining the active sludge of the sewage treatment plant, marking line for separating through dilution and accumulation culture, screening translucent, thick and smooth-raised single colony with excellent growth, ensuring the strain is relatively pure through the microscopic examination, finally re-screening the primarily selected strain through the flocculating effect test to obtain strains for generating the flocculating agent, preparing the seed culture medium with pH of 6-8 using water (in parts weight) (1000), beef extract (2-4), peptone (7-12) and table salt (4-6), inoculating the strain for generating the flocculating agent into the seed culture medium, fermenting at 25-35 degrees C and 120-150 rpm for 24 hours, where the fermentation liquid has optical density of 1.4-1.6, adding glucose (0-4 g) as replenished carbon source and ammonium sulfate (0-1 g) as replenished nitrogen source to the culture wastewater (1 l) having chemical oxygen demand (COD) of 1000-10000 mg/l to obtain the culture wastewater fermentation culture medium with original pH of 6-8, sterilizing at high pressure, inoculating the seed liquid to the culture effluent fermentation culture medium, where 100 ml of the culture wastewater fermentation culture medium contains 0.5-2 ml of the strain seed liquid, finally culturing by aerobic fermentation at 25-35 degrees C and 120-150 rpm for 36-48 hours, and collecting the supernatant containing the microbial flocculating agent product.