NOVELTY - Removing 2,4-dichlorophenol in water with polyurethane sponge immobilized white rot fungus comprises: (a) pre-treating carrier; and (b) immobilizing the white rot fungus and removing 2,4-dichlorophenol in water.    USE - The method is useful for removing 2,4-dichlorophenol in water with polyurethane sponge immobilized white rot fungus (claimed).    ADVANTAGE - The carrier has porosity, hydrophilicity and strong adsorption; the chemical performance is stable, it will not dissolve in acid/alkaline aqueous solution, thus the hydrolysis resistance is strong; the biological bearing amount and mass transfer rate are high, the compatibility is good. The white rot fungus can be effectively absorbed and immobilized by immobilizing the carrier, thus the ability of removing the 2,4-dichlorophenol in water with the immobilized white rot fungus is improved, and it is easy to separate the immobilized white rot fungus from the product. The carrier is suitable for treating sludge.    DETAILED DESCRIPTION - Removing 2,4-dichlorophenol in water with polyurethane sponge immobilized white rot fungus comprises: (a) pre-treating carrier: (i) cutting polyurethane sponge into cuboid material with size of 2x1x1 cubic meter, soaking in ethanol solution with volume percent of 70 percent for 4 hours at room temperature for removing sponge scrap; (ii) washing the above polyurethane sponge with ultra-pure water for three times, adding 2 blocks of sponge into per 100 ml of inorganic salt liquid culture medium; (iii) treating with ultrasonic wave for 30 minutes; and (iv): sterilizing at high temperature of 121 degrees C for 20 minutes, after finishing sterilizing, cooling to room temperature for obtaining polyurethane sponge carrier for immobilizing; and (b) immobilizing the white rot fungus and removing 2,4-dichlorophenol in water: (i) thawing the white rot fungus strain stored at low temperature of -80 degrees C via raising the temperature in a gradient manner, standing until the room temperature is reached, then introducing into inorganic salt liquid culture medium under sterile condition; (ii) putting a tapered bottle which is filled with inorganic salt liquid culture medium into a constant-temperature rocking bed for standing for 12 hours for immobilizing white rot fungus and carrier for obtaining immobilized white rot fungus; (iii) improving the rotating rate of the rocking bed, culture via vibrating at constant temperature; (iv): culturing the strain until the logarithm growth prophase is reached, then adding into waste water which contains 2,4-dichlorophenol into the tapered bottle which is filled with inorganic salt liquid culture medium; and (v) measuring with a high performance liquid chromatograph the residua of the 2,4-dichlorophenol in 12 hours, centrifuging for 20 minutes at rate of 6000 revolutions per minute, then taking the supernatant.