NOVELTY - Producing banana plantlet by tissue culture method, involves mixing organic substrate and inorganic substrate to prepare culture substrate for plantlet, adding the substrate in nutrient solution, immersing the root portion of plantlet in mixture, grading the plantlet and transplanting the plantlet in furrows, placing the plantlets in small area and covering the plantlet with plastic films, adding the nutrient solution to the plantlet after the first leaf of the plantlet appears, and maintaining the water and light for hardening of plantlet.    USE - The method is useful for producing banana plantlet by tissue culture method.    ADVANTAGE - The substrate develops root system, has good permeability, avoids damages caused by bacteria and pest, and is environment-friendly. The survival rate of banana is 94-100% higher than the soil culture.    DETAILED DESCRIPTION - Method of producing banana plantlet by tissue culture method, involves (a) preparing culture substrate by mixing organic substrate and inorganic substrate at volume ratio of 1:1, (b) preparing nutrient solution (organic fertilizer) (0.1-0.3%) using nitrogen, phosphorus and potassium fertilizer at the ratio of 1:0.4:3.5, (c) uniformly mixing the organic substrate and inorganic substrate at the volume ratio of 1:1 as the culture substrate, adding organic fertilizer (40 kg) for every cubic meter of the culture substrate, (d) adding the mixed substrate in nutrient medium, (e) using clean water to clearly wash the substrate adhering to the root portion of the hardened plantlet root, immersing the root portion of the banana plantlet in mixture containing liquid (600 times) with respect to carbendazim for 1 minute, grading the plantlet and transplanting the plantlet with different height in furrows, using non-polluted clean water to entirely water the substrate for normal root, placing the plantlet in the small area and covering using plastic films, (f) watering the plantlets after 7 days of transplantation and uncovering the covered area with plastic films, (g) adding the nutrient solution (0.1%) twice a week after the appearance of first leaf, increasing the concentration of the nutrient solution to 0.2-0.3% after 10 days, when the plantlet entirely changes to green, and (h) ceasing the addition of nutrient solution 10 days before the plantlet appears, and maintaining the water and light for hardening of plantlets.