NOVELTY - A recombinant vector pGAprEHS of expression protein comprising: (a) the genome of the Bacillius subtilis FZB42 as a template; (b) the Bacillius subtilis-Escherichia coli protein shuttle expression vector pM43HF plasmid; and the plasmid pIC333 as a template, is new.    USE - The recombinant vector pGAprEHS of expression protein and biological product of Bacillius subtilis gene engineering bacteria of expression Harpin protein are useful for accelerating the growth of plants and controlling Pyricularia grisea, Thanatephorus cucumeris, Blumeria graminis, wheat scab, tomato bacterial wilt, Alternaria solani, Phytophthora capsici leonian, pepper anthracnose, pepper viral disease, cucumber root rot, cucumber botrytis, cucumber downy mildew, qreens downy mildew and aphid (claimed).    ADVANTAGE - The protein is safe and environment friendly.    DETAILED DESCRIPTION - A recombinant vector pGAprEHS of expression protein comprising: (a) the genome of the Bacillius subtilis FZB42 as a template, using primers aprE-1 and aprE-2 to augment aprE gene and sequence 2815bp on its two sides, the program of the augment as 95 degrees C, 4 minutes; 94 degrees C, 45 seconds; 55 degrees C, 30 seconds; 72 degrees C, 2 minutes; 30 circulations; 72 degrees C, 10 minutes; connecting the augmented segments with pGEM-T-esay cloning carrier to construct successfully pGEM-easy-T-aprE; aprE-1atagtaaggcatcaacc (SEQ ID NO. not defined); aprE-2cggtcgtgattacctttata; (b) the Bacillius subtilis-Escherichia coli protein shuttle expression vector pM43HF plasmid designed by the lab itself as a template, the primers p43-1(HapI) and Hrp-2(ClasI) to augment p43+SP+hrf2 gene sequence, the size as 813bp; the primers p43-1(HapI) and Hrp-2(ClasI) lead in HapI and ClasI restriction enzyme cutting site separately from two ends of the augmented sequences, the program of the augment as 95 degrees Cd, 4 minutes; 94 degrees C, 45 seconds; 62 degrees C, 30 seconds; 72 degrees C, 1 minutes; 30 circulations; 72 degrees C, 10 minutes; double cutting the augmented segments by the HapI and ClasI and connected to the pGEM-easy-T-aprE vector and successfully constructing pGEM-easy-T-aprE- p43sphrf2 intermediate vector; p43-1(HaPI) aactgataggtggtatgttttc (SEQ ID NO. not defined); Hrp-2(ClaI)ttttatcgatctattactgcattcatgcgc (SEQ ID NO. not defined); and (c) the plasmid pIC333 as a template, using the primers spe-1-lox66 and spe-2-lox71 to augment antibiotic (Spectinomycin) marker gene sequence consisting of lox sequence locus, the primers spe-1-lox66 and spe-2-lox71 leading in lox66 and lox71 locus separately from the augment sequence, the program of augment as 95 degrees C, 4 minutes; 94 degrees C, 45 seconds; 62 degrees C, 30 seconds; 72 degrees C, 2 minutes; 30 circulations; 72 degrees C, 10 minutes; the attained by augment coding gene segment as 1438np size; spe-1-lox66: fully defined 57 bp (SEQ ID NO. not defined); spe-2-lox71: fully defined 56 bp (SEQ ID NO. not defined); connecting the antibiotic screening gene of the Pfu enzymantic amplification to the intermediate vector pGEM-easy-T-aprE-p43sphrf2 which is enzyme digested by restriction enzyme to successfully construct a recombinant vector pGAprEHS of expression protein, is new. INDEPENDENT CLAIMS are: (1) a Bacillius subtilis FZB42nprE gene targeted mutagenesis carrier pUNprEK; (2) a Bacillius subtilis gene engineering bacteria of the expression Harpin protein constructed by the recombinant vector pGAprEHS of expression Harpin protein and the Bacillius subtilis FZB42nprE gene targeted mutagenesis carrier pUNprEK; (3) a constricting method of the Bacillius subtilis gene engineering bacteria of the expression Harpin protein; (4) a biological product of Bacillius subtilis gene engineering bacteria of expression Harpin protein; and (5) a preparation method of the biological product of Bacillius subtilis gene engineering bacteria of expression Harpin protein.