NOVELTY - Preparation of biodiesel using pupal oil comprises extracting powdered silkworm chrysalis, mixing extract with sodium hydroxide, stirring, saponifying, and cooling; extracting fatty acid, standing, washing to neutrality, drying, filtering, and recycling solvent to obtain mixed fatty acid; mixing mixed fatty acid with saturated urea solution with low-carbon alcohol, stirring, crystallizing, and filtering; extracting filtrate, washing, drying extract, and distilling; and adding solid catalyst to residue, stirring, esterifying, filtering, standing and distilling upper layer.    USE - Method of preparing biodiesel using pupal oil (claimed).    DETAILED DESCRIPTION - Preparation of biodiesel using pupal oil comprises removing impurities from silkworm chrysalis, and crushing into powder; extracting powdered silkworm chrysalis in petroleum ether or n-hexane by heat refluxing to extract crude pupal oil, mixing obtained extract with ethanol solution of sodium hydroxide (1-10 wt.%), stirring, saponifying under water bath at 30-90 degrees C for 1-12 hours, cooling to room temperature, adding petroleum ether or n-hexane to remove unsaponified substance, and adding hydrochloric acid solution (5-20 wt.%) in the saponified fluid to obtain non-esterified fatty acid having pH value of 3-4; extracting fatty acid using petroleum ether or n-hexane, standing, combining petroleum ether or n-hexane layer, washing to neutrality, drying, filtering, and recycling the ether or n-hexane to obtain mixed fatty acid; mixing mixed fatty acid with saturated solution of urea with low-carbon alcohol to obtain clathrate compound, stirring, cooling the compound to crystallize, and filtering; extracting filtrate with petroleum ether or n-hexane, washing, drying extract, and distilling the solution under reduced pressure to obtain unsaturated fatty acid product; and putting obtained residue in a reactor, adding solid catalyst, stirring, adding methyl alcohol, esterifying at 30-90 degrees C, filtering reaction liquid, recycling the catalyst, standing, and distilling upper layer under reduced pressure.