NOVELTY - An antagonistic bacteria a45 for preventing hot pepper bacterial wilt, belonging to Bacillus subtilis deposited as CGMCC No.3480; where the main biological characteristics is as follows: it is Gram-positive; the cell is rod-shaped; it can ferment glucose, fructose, arabinose, mannitose, and mannitol to generate acid and can hydrolyze starch and liquefy glutin; the citrate, the nitrate reduction reaction, and Voges-Proskauer (V-P) reaction test are positive; and the methyl red reaction, the malonic acid test, and the tartrate test are negative; is new.    USE - The antagonistic bacteria a45 and the microbial organic fertilizer are useful for preventing hot pepper bacterial wilt (all claimed).    ADVANTAGE - The experimental result shows that the microbial organic fertilizer, after being applied to the soil, enables the antagonistic bacteria to grow quickly and further form a superior group in the soil. Moreover, the prevention rate to the hot pepper bacterial wilt reaches 70%.    DETAILED DESCRIPTION - An antagonistic bacteria a45 for preventing hot pepper bacterial wilt, belonging to Bacillus subtilis deposited as CGMCC No.3480; where the main biological characteristics is as follows: it is Gram-positive; the cell is rod-shaped; the spore is mesic or nearly mesic and is elliptical; the sporocyst is not expanded, which does not have the parasporal crystal; it can ferment glucose, fructose, arabinose, mannitose, and mannitol to generate acid and can hydrolyze starch and liquefy glutin; the citrate, the nitrate reduction reaction, and Voges-Proskauer (V-P) reaction test are positive; the methyl red reaction, the malonic acid test, and the tartrate test are negative; and after performing the cultivation on the Luria-Bertani (LB) flat plate at 30 degrees C for 24 hours, the colony is irregular in periphery and opaque; is new. An INDEPENDENT CLAIM is a microbial organic fertilizer produced using the antagonistic bacteria a45 for preventing hot pepper bacterial wilt.