TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation: No preparation method is given. Preferred Components: The nucleic acid comprises a segment of at least 50 nucleotides of SEQ ID NOs: 91-102 (Sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication). The coding sequence comprises a plastid targeting sequence in-frame with a fatty acyl-acyl carrier protein (ACP) thioesterase, which has hydrolysis activity towards at least one 8C-14C fatty acyl-ACP substrate. The plastid targeting sequence encodes a peptide that is capable of targeting a protein to the plastid of a cell of Prototheca. The plastid targeting sequence is from microalgae. The plastid targeting sequence has at least 25% amino acid sequence identity to at least one of SEQ ID NO: 127-133 (Sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication) and is capable of targeting a protein to the plastid of a cell of Prototheca. The vector further comprises a nucleic acid (first and second) sequence endogenous to the nuclear genome of a cell of Prototheca, where the first and second sequences: (a) are each at least 200 nucleotides long; (b) flank the expression cassette; and (c) are located on the same Prototheca chromosome no more than 20 kB apart. The fixed carbon source is depolymerized cellulosic material consisting of corn stover, Miscanthus, forage Sorghum, sugar beet pulp and sugar cane bagasse. The fixed carbon source is depolymerized cellulosic material that has been subjected to washing with water prior to the culturing step. The glucose level of the depolymerized cellulosic material is concentrated to a level of at least 300 g/l, preferably at least 600 g/l of prior to the culturing step and is fed to the culture over time as the cells grow and accumulate lipid. The exogenous gene encodes the fatty acyl-ACP thioesterase. The triglyceride composition (oil) has a profile of at least 4% 8-14C, and at least one of: less than 0.4 mu g/ml total carotenoids; less than 0.001 mu g/ml lycopene; less than 0.02 mu g/ml beta carotene; less than 0.02 mg of chlorophyll/kg of oil; 0.4-0.6 mg of gamma -tocopherol/100 g of oil; 3-9 mg campesterol/100 g of oil; and less than 0.5 mg of total tocotrienols/g of oil. The microorganisms are a species of Prototheca and contain an exogenous gene. Preferred Method: The chemical manufacturing further comprises manufacturing the oil by cultivating a cell of Prototheca that comprises an exogenous fatty acyl-ACP thioesterase gene encoding the fatty acyl-ACP thioesterase having hydrolysis activity. The method of chemical manufacturing comprises performing a hydrolysis reaction. The hydrolysis reaction comprises a hydrolysis reaction consisting of saponification, catalytic hydrolysis (both preferred), acid hydrolysis, alkaline hydrolysis, enzymatic hydrolysis or hot-compressed water hydrolysis, where the oil is split into glycerol and fatty acids. The fatty acids formed undergo: an amination reaction to produce fatty nitrogen compounds; and an ozonolysis reaction to produce mono- and dibasic-acids. The oil undergoes a triglyceride splitting method consisting of enzymatic splitting or pressure splitting. In the method of chemical manufacturing, a condensation reaction follows the hydrolysis reaction. The product of the hydroprocessing reaction undergoes a deoxygenation reaction. The oil undergoes a deoxygenation reaction prior to the performance of a hydroprocessing reaction. The oil undergoes a hydroprocessing reaction and a deoxygenation reaction simultaneously. The oil manufacturing method further comprises an isomerization method and a gas removal reaction, where a condensation reaction follows the hydroxylation reaction, esterification reaction, the hydroprocessing reaction or the deoxygenation reaction. The esterification reaction comprises an interesterification reaction. The esterification reaction comprises a transesterification reaction. EXAMPLE - No suitable example given.