NOVELTY - A yeast culture comprises at least two recombinant strains of yeast where the culture produces a designer cellulosome, and where the yeast culture catabolizes cellulosic material to produce ethanol.    USE - For producing ethanol; and for designing a cellulsome for producing a product from cellulosic material i.e. a biofuel metabolite (claimed).    ADVANTAGE - The yeast strains enables for direct fermentation of cellulose to ethanol, eliminating the need for use of purified cellulases. The methods and compositions provide abundant, low-cost, agriculture residue to be used as raw material for ethanol production. The increased production of ethanol not only reduces pollution to the environment but also the need for imported petroleum as transportation fuel. The method is at least efficient, economical, and environmentally friendly conversion of biomass. Current biofuel production processes are exclusively based upon the soluble enzyme approach, the less efficient model utilized by aerobic microbes. The construction of cellulosomes on the cell surface in present method departs from the current model of enzymatic hydrolysis. A difference lies in the extent of synergism. While the secretion or co-display of cellulases (without the cellulosome structure) permits a synergistic use of these enzymes to some extent, both follow the model of aerobic organisms in cellulose hydrolysis. Since ethanol production is carried out anaerobically, the limited amount of enzyme production makes the high coordination of different enzyme components necessary to maximize the synergy and efficiency.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following:    (1) a culture (C1) comprising: a first recombinant yeast strain comprising an anchoring scaffoldin (anScaff); a second recombinant yeast strain comprising an adaptor scaffoldin comprising several cohesin domains and at least one cellulose binding domain (CBD); and at least one recombinant yeast strain comprising several of secreted dockerin-tagged cellulases;    (2) new recombinant yeast strain (S1) comprising a heterologous polynucleotide encoding an anchoring scaffoldin;    (3) new recombinant yeast strain (S2) comprising a heterologous polynucleotide encoding an adaptor scaffoldin comprising several cohesin domains and at least one cellulose binding domain (CBD);    (4) new recombinant yeast strain (S3) comprising at least one heterologous polynucleotide encoding a secreted dockerin-tagged cellulose;    (5) a culture comprising a recombinant yeast strain (S1), (S2) or (S3);    (6) designing a cellulsome involving identifying the cellulosic substrate, identifying at least one enzyme useful for degradation of the cellulosic material, recombinantly engineering a dockerin peptide to the enzyme, cloning a polynucleotide encoding the dockerin-linked enzyme into a microorganism, culturing the microorganism in a culture of at least one additional microorganism expressing a scaffoldin having several cohesion domains and a cellulosic binding domain, where the cohesion and dockerin a compatible and culturing the microorganisms to express the scaffoldin and dockerin-linked enzymes;    (7) producing a product from cellulosic material involving culturing designed cellulosome where the cellulosic material to produce the product;    (8) producing a biofuel metabolite involving: culturing the yeast in a fermentation broth comprising a cellulosic material, where the microorganism produces the biofuel metabolite.