TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: In extracting a lipid from a microorganism, treating comprises treating with more than 5% v/v of an organic solvent to the lysate. The layers further comprise a lipid:aqueous emulsion layer and/or a cell pellet. Lysing a cultured microorganism to produce a lysate is performed at an acidic pH. It also comprises one or more methods of lysing the microorganism selected from heating, sonication, mechanical lysis, osmotic shock, expression of an autolysis gene, exposure to pH above 8, exposure to pH below 6, or digestion with an enzyme. The microorganism produces a lipid that comprises at least 25% w/v of total cellular lipid as 18C triacylglycerols, at least 25% w/v of total cellular lipid as 16C triacylglycerols, at least 10% w/v of total cellular lipid as 14C triacylglycerols, at least 10% w/v of total cellular lipid as 12C triacyl glycerols, and at least 10% w/v of total cellular lipid as 10C triacylglycerols. The microorganism produces a lipid with a lipid profile comprising at least 4% 8-14C. The microorganism produces a lipid with a lipid profile comprising 4-40% 10-14C. Lysing a cultured microorganism to produce a lysate also comprises acidic lysis and heating. The organic solvent comprises an oil selected from oil from soy, rapeseed, canola, palm, palm kernel, coconut, corn, waste vegetable, Chinese tallow, olive, sunflower, cotton seed, chicken fat, beef tallow, porcine tallow, microalgae, macroalgae, Cuphea, flax, peanut, choice white grease, lard, Camelina sativa, mustard seed cashew nut, oats, lupine, kenaf, Calendula, hemp, coffee, linseed, hazelnut, Euphorbia, pumpkin seed, Coriander, Camellia, sesame, safflower, rice, tung oil tree, cocoa, copra, pium poppy, castor beans, pecan, jojoba, Jatropha, macadamia, Brazil nuts, avocado, a fossil oil, or a distillate fraction. The organic solvent is a surfactant. Separating the lysate into layers comprises reducing the temperature of the mixture below 25 degrees C. The microorganism is selected from a bacterium, a cyanobacterium, eukaryotic microalgae, oleaginous yeast, or fungus. The microorganism is of the genus Chlorella or Prototheca. The method further comprises: (d) removing the lipid:organic solvent composition from the other layer(s), which is performed without substantially separating the lipid from the organic solvent composition. In producing fuel, the chemical reaction is trans-esterification and the fuel is biodiesel. The biodiesel meets or exceeds the ASTM D6751 biodiesel standard. It also meets or exceeds the EN 14214 biodiesel standard. The chemical reaction comprises one or more reactions selected from hydroprocessing, deoxygenation, or isomerization, and the fuel is renewable diesel. The renewable diesel meets or exceeds the ASTM D 975 standard. The chemical reaction comprises hydrotreating and the fuel is jet fuel. The microorganism has not been subjected to a drying step. Subjecting the lipid:organic solvent composition to a chemical reaction is performed without substantially separating the lipid from the organic solvent. The microorganism has at least 85% 23S rRNA genomic sequence identity to one or more sequences selected from SEQ ID NO. 7-39. EXAMPLE - No suitable example given.