TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation: No preparation method is given. Preferred Components: In the transgenic cell, at least 2 endogenous nucleotide sequences are disrupted, where the disrupted endogenous nucleotide sequences include pilT (SEQ ID NO: 1), pilA (SEQ ID NO: 2), nirS (SEQ ID NO: 3), bdlA (SEQ ID NO: 4), lasI (SEQ ID NO: 5), lasR (SEQ ID NO: 6), ftsZ (SEQ ID NO: 7), or fliC (SEQ ID NO: 8). In the transgenic cell, at least two disrupted endogenous nucleotide sequences include group of double nucleotide sequences comprising: pilT (SEQ ID NO: 1) and bdlA (SEQ ID NO: 4); bdlA (SEQ ID NO: 4) and nirS (SEQ ID NO: 3); bdlA (SEQ ID NO: 4) and lasI (SEQ ID NO: 5); nirS (SEQ ID NO: 3) and pilT (SEQ ID NO: 1); nirS (SEQ ID NO: 3) and lasI (SEQ ID NO: 5); lasI (SEQ ID NO: 5) and pilT (SEQ ID NO: 1); ftsZ (SEQ ID NO: 7) and pilT (SEQ ID NO: 1); ftsZ (SEQ ID NO: 7) and bdlA (SEQ ID NO: 4); ftsZ (SEQ ID NO: 7) and nirS (SEQ ID NO: 3); or ftsZ (SEQ ID NO: 7) and lasI (SEQ ID NO: 5). In the transgenic cell, at least three endogenous nucleotide sequences are disrupted and the at least three disrupted endogenous nucleotide sequences include group of triple nucleotide sequences comprising: pilT (SEQ ID NO: 1), bdlA (SEQ ID NO: 4) and ftsZ (SEQ ID NO: 7); bdlA (SEQ ID NO: 4), nirS (SEQ ID NO: 3) and ftsZ (SEQ ID NO: 7); bdlA (SEQ ID NO: 4), lasI (SEQ ID NO: 5) and ftsZ (SEQ ID NO: 7); nirS (SEQ ID NO: 3), pilT (SEQ ID NO: 1) and ftsZ (SEQ ID NO: 7); nirS (SEQ ID NO: 3), lasI (SEQ ID NO: 5) and ftsZ (SEQ ID NO: 7); lasI (SEQ ID NO: 5), pilT (SEQ ID NO: 1) and ftsZ (SEQ ID NO: 7); pilT (SEQ ID NO: 1), bdlA (SEQ ID NO: 4) and nirS (SEQ ID NO: 3); pilT (SEQ ID NO: 1), bdlA (SEQ ID NO: 4) and lasI (SEQ ID NO: 5); or bdlA (SEQ ID NO: 4), lasI (SEQ ID NO: 5) and nirS (SEQ ID NO: 3). In the transgenic cell, at least four endogenous nucleotide sequences are disrupted and the at least four disrupted endogenous nucleotide sequences include group of quadruple nucleotide sequences comprising: pilT (SEQ ID NO: 1), bdlA (SEQ ID NO: 4), nirS (SEQ ID NO: 3) and ftsZ (SEQ ID NO: 7); pilT (SEQ ID NO: 1), bdlA (SEQ ID NO: 4), lasI (SEQ ID NO: 5) and ftsZ (SEQ ID NO: 7); bdlA (SEQ ID NO: 4), lasI (SEQ ID NO: 5), nirS (SEQ ID NO: 3) and ftsZ (SEQ ID NO: 7), or pilT (SEQ ID NO: 1), bdlA (SEQ ID NO: 4), lasI (SEQ ID NO: 5) and nirS (SEQ ID NO: 3). In the transgenic cell, at least five endogenous nucleotide sequences are disrupted and the at least five disrupted endogenous nucleotide sequences include group of quintuple nucleotide sequences comprising pilT (SEQ ID NO: 1), bdlA (SEQ ID NO: 4), lasI (SEQ ID NO: 5), nirS (SEQ ID NO: 3), and ftsZ (SEQ ID NO: 7). The matrix includes sponges, filters, beads, powders, tissues, cassettes, cartridges, or capsules.    TECHNOLOGY FOCUS - INSTRUMENTATION AND TESTING - Preferred Components: The filtration device comprises an indicator. In the microbial fuel cell, the anode chamber is detachable. The biofilm is attached to the anode, where the biofilm is exposed to a feedstock including sewage, fertilizer run-off, waste-water, animal waste, gaseous material, or a greenhouse gas. In the microbial fuel cell, anaerobic conditions are maintained around the biofilm. The anodic chamber is pretreated with a biofilm inhibitor. The microbial fuel cell further comprises: a mediator; an ultracapacitor; a water transfer component, where the water transfer component transfers water produced by the microbial fuel cell to a water collection device or to the external environment; a hydrogen transfer component; and a hydrogen fuel cell. The sensor further comprises a second transgenic bacterial cell for detecting a second predetermined compound. EXAMPLE - No suitable example given.