TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Fertilizer: In the green environment-friendly biological organic composite fertilizer, the mutagenesis, separation and purification of strain comprises: (a) preparing gravy culture medium; (b) sterilizing the gravy culture medium for 30 min at 1.5 kg/square centimeter; (c) putting more than one ring of strains in the gravy culture medium under sterile condition; (d) micro-vibrating the gravy culture medium, adding micro-amount of antibacterial streptomycin, uniformly mixing; (e) pouring the culture medium uniformly mixed with sample for separating in a culture dish, uniformly shaking; and (f) radiating with ultraviolet radiation and culturing pure breed; firstly, starting ultraviolet ray lamp to pre-heat for 15 minutes, after ultraviolet ray lamp is stable, putting the culture dish in dark room or sterile room, radiating for 1-3 minutes (decided according to the activity of the strain) at the position about 15-25 cm far away the ultraviolet ray lamp, at the same time, opening red lamp or other color lamps, after finishing radiating, coating the treated culture dish with black cloth or other dark color cloth, putting the culture dish in a culture tank, culturing for 24-72 hours at constant temperature of 28-30 degrees C, selecting eugonic strain with high activity, inoculating the strain in a test tube inclined-plane culture medium to culture, repeating performing mutagenesis, separation and purification, until effective strain with high activity and without fungi is screened from the test tube or the culture dish, inoculating the strain in inclined-plane test tube as production strain or putting the strain in a vacuum refrigeration drying ampoule to store for a long time. The preparation and fermentation of the strain and microbial inoculum culture medium comprises (a) preparing AS4.891Steptomyces miroftauus fertilizer: (i) preparing inclined-plane strain by 2 g of soluble starch, 1 g of calcium carbonate, 0.05 g of dipotassium phosphate (K2HPO4), 0.05 g of sodium chloride, 0.001 g of ferric sulfate (FeSO4), 1.5 g of agar and 100 ml of distilled water, where the pH value is 7.2-7.4; and (b) 0.05 g of K2HPO4, 0.05 g of magnesium sulfate (MgSO4), 0.5 g of calcium carbonate (CaCO3), 20-40 parts of bean cake powder, 50-80 parts of fertile soil, 25-50 parts of straw powder, 0.5-1.5 parts of superphosphate, and 3-6 parts of grass peat or plant ash or fly ash, using calcium oxide to regulate pH to about 7-7.5; sterilizing, inoculating and culturing method of the culture medium are as follows: sterilizing culturing medium: uniformly mixing the inclined-plane culture medium, secondary microbial inoculum solid culture medium cultivate material, filling the inclined-plane culture medium to the test tube, placing the left secondary culture medium to the tin container or triangular flask or sac, covering the opening or the upper part with cotton or multi-layer gauze or kraft paper, placing the culture medium in the sterilizing pot, sterilizing for 30 minutes at pressure of 1.5 kg/cm2; inoculation and culture method of the culture medium is: when cooling the culture medium to 40 degrees C or lower, inoculating the test tube inclined-plane culture medium to the mother strain or activating Ampoule dry freezing pipe strain, inoculating secondary culture medium to the inclined-plane or triangular flask strain, controlling temperature at 25-28 degrees C, fermenting for 48-72 hours, detecting the microbial inoculum by microscope, if qualified using as production microbial inoculum of bacterial manure; and (ii) the preparation of the AS1.910 Bacillus mucilaginous; and (b) AS4.891Steptomyces miroftauus microbial inoculum: (i) preparing inclined-plane strain by 0.02-0.04 g of monopotassium phosphate (KH2PO4), 0.05-0.1 g of the K2HPO4, 0.01-0.02 g of magnesium sulfate, 0.005-0.015 g of calcium sulfate (CaSO4), micro amount of ferric chloride (FeCl), micro amount of sodium aluminate, 0.05 g of yeast extractive, 2 g of mannite, 1.5 g of agar and 100 ml of the distilled water, where the pH value of the solution is 7.2; (ii) second-level strain solid culture medium (part by weight) comprising: 0.01-0.02 g of the monopotassium phosphate KH2PO4, 0.04-0.1 g of the K2HPO4, 0.01-0.02 g of MgSO4.7H2O, 0.005-0.015 g of CaSO4.2H2O, 20-60 parts of rice bran (wheat bran or cotton seed cake), 20-40 parts of crop straw powder, 20-40 parts of fertile soil, 0.5-1.5 parts of superphosphate and 3-6 parts of grass peat or plant ash or fly ash, using calcium oxide to regulate pH to about 7-7.5; sterilizing, inoculating and culturing method of the culture medium are as follows: sterilizing culturing medium: uniformly mixing the inclined-plane culture medium, secondary microbial inoculum solid culture medium cultivate material, filling the inclined-plane culture medium to the test tube, placing the left secondary culture medium to the tin container or triangular flask or sac, covering the opening or the upper part with cotton or multi-layer gauze or kraft paper, placing the culture medium in the sterilizing pot, sterilizing for 30 minutes at pressure of 1.5 kg/cm2; inoculation and culture method of the culture medium is: when cooling the culture medium to 40 degrees C or lower, inoculating the test tube inclined-plane culture medium to the mother strain or activating Ampoule dry freezing pipe strain, inoculating secondary culture medium to the inclined-plane or triangular flask strain, controlling temperature at 25-28 degrees C, fermenting for 48-72 hours, detecting the microbial inoculum by microscope, if qualified using as production microbial inoculum of bacterial manure. The preparation and processing of the organic fertilizer comprises: (a) preparation and making of humic acid fertilizer; (i) preparing 60-100 parts of weathered coal or peat or grass carbon; (ii) weighing 10-30 parts of diluted sulfuric acid with a specific gravity of about 1.28 and mixing it with appropriate water; (iii) mixing the weathered coal or peat or grass carbon with the mixture of the diluted sulfuric acid and water; uniformly stirring the system for 24-72 hours of acidification treatment; then mixing the acidified weathered coal or peat or grass carbon with 15-30 parts of powdered phosphate rock or phosphorus fertilizer and uniformly stirring them; stacking the mixture to have a reaction for 5-6 days to obtain humic acid phosphorus fertilizer; and (b) preparation and making of organic crude fertilizer (including urban garbage or animal waste or skin leftovers or subsidiary agricultural product leftovers and a variety of cake materials): (i) stacking the residue from urban garbage after magnetic separation and classification for 8-15 days of natural fermentation; then passing the residue through a sieve of 10-30 meshes; the filtered substances being organic matters and humus, which can be good organic fertilizer after being baked or dried; (ii) adding a part of grass meal or sawdust or weathered coal to the animal wastes for 5-10 days of natural fermentation or adding a third-level microbial inoculum of biological organic phosphorus bacterial fertilizer to the animal wastes; then baking or drying the fermented product in the air to obtain good organic fertilizer; and (iii) crushing the animal skin leftovers and passing the crushed powders through a sieve of 10-30 meshes; then adding microbial inoculum of biological nitrogen-fixing bacteria and third-level microbial inoculum of biological organic phosphorus bacterial fertilizer to the powders to ferment for 4-6 days; then baking or drying the fermented product in the air to obtain good organic protein fertilizer. The preparation and processing of the inorganic fertilizer comprises: (a) preparing 30-60 parts by weight of inorganic materials, in which the mixture ratio of nitrogen to phosphorus to potassium is (3-8):(2-6):(1-5-1) adding 5-19 parts by weight of urea accounting for 46.6%; (b) adding 20-50 parts by weight of phosphorus accounting for 14%; (c) adding 6-14 parts by weight of potassium chloride accounting for 58%; and (d) preparing 2-8 parts by weight of trace element, including 0.5-2.5% of MgSO4, 0.5-2.5% of CaCO3, 0.05-0.15% of boric acid, 0.15-0.25% of zinc sulfate, 0.25-0.35% of manganese sulfate, 0.005-0.15% of ammonium molybdate, 0.5-0.15% of copper sulfate, 0.15-0.25% of fulvate, and 0.04-0.095% of rare earth; and finally granulating the mixture adding the organic fertilizer and humic acid phosphorus fertilizer or fly ash to a mixer; then adding biological bacteria fertilizer and uniformly stirring the fertilizer; subsequently adding the inorganic matter to the system and uniformly mixing them; sending the well mixed compound fertilizer to a granulator by a conveyer for granulating; finally drying the grains with air flow or baking them at a low temperature to obtain the high-efficiency organic biological full-element compound grain fertilizer. ACTIVITY - Fertilizer.