NOVELTY - Cultivating method for degrading microbial strains in industrial waste water comprises preparing liquid cultivation medium of objective compound with different concentration gradient; acquiring strain with plasmid regarded as unique carbon source and nitrogen source of aniline or nitrobenzene compound, and regarded as unique carbon source of phenol; cultivating and evaluating the plasmid and the degradation ability; and acquiring industrial waste water degradation microbial strains by purifying.    USE - The method is useful for degrading microbial strains in industrial waste water (claimed).    ADVANTAGE - The method is simple, inexpensive, capable of degrading composite pollutant by the cultivated strain, and has high degradation efficiency to aniline, phenol and nitrobenzene compound in industrial waste water.    DETAILED DESCRIPTION - Cultivating method for degrading microbial strains in industrial waste water comprises (a) preparing liquid cultivation medium of objective compound with different concentration gradient for aniline, phenol and nitrobenzene compound in industrial waste water; the liquid cultivation medium is regarded as unique carbon source and nitrogen source of aniline or nitrobenzene compound, the phenol is additionally added with nitrogen source; the liquid cultivation medium is further added with other chemical base components for growing the microbial strains; and conversing partial liquid cultivation medium into selective solid cultivation medium; (b) adding beef extract substrate liquid cultivation medium into active pollution mud in the industrial waste water to gather them; then inoculating them into objective compound with different concentration gradient to perform cultivation; the cultivation temperature is 15-45 degrees C; the cultivation time is 6-25 days; the repeated cultivation is 2-12 times; coating cultivated strain on the selective solid cultivation medium to perform plate streaking separation, and extracting plasmid, to acquire strain with plasmid regarded as unique carbon source and nitrogen source of aniline or nitrobenzene compound, and regarded as unique carbon source of phenol; (c) inoculating Pseudomonas elodea into beef extract substrate liquid cultivation medium to gather them; preparing them into competent cell after being gathered; heavily hanging three plasmid strains gained in step (b) and the competent cell in the Luria Bertani (LB) liquid cultivation medium; hatching 10-45 hours under 10-40 degrees C; then adding them into objective compound with different concentration gradient to perform cultivation; the cultivation temperature is 15-45 degrees C; the cultivation time is 6-25 days; the repeated cultivation is 2-12 times; coating cultivated strain on the selective solid cultivation medium after the cultivation; hatching 10-45 hours under 10-40 degrees C; then evaluating the plasmid and the degradation ability; and (d) purifying the strain acquired in step (c), and acquiring industrial waste water degradation microbial strains with plasmid.