TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: Producing carbohydrate-enriched biomass further comprises: (e) washing the biomass produced in (d) with a solvent solution; (f) washing the biomass produced in (e) with water to produce readily saccharifiable carbohydrate-enriched biomass; (g) drying the biomass produced in (e) to produce readily saccharifiable carbohydrate-enriched biomass; and (h) repeating (e) and (f) one or more times. The heating step of (c) occurs in a sealed pressure vessel, and the filtering step of (d) occurs under pressure. The organic solvent solution further comprises an additional nucleophile selected from ammonia (NH3), sodium hydroxide (NaOH), one or more alkylamines, sulfide reagents, and combinations. The additional nucleophile is one or more alkylamine and the one or more alkylamine is at a concentration of up to 20% by weight of dry biomass. The solvent solution to biomass in (b) has a weight ratio of 10-1-0.5-1. The heated suspension of (c) is cooled to room temperature before filtering in (d). The method as well comprises: evaporating off the solvent under vacuum of the filtered and washed biomass after (e); saccharifying the biomass with an enzyme consortium where fermentable sugars are produced; saccharifying the biomass without drying by contacting the biomass with an enzyme consortium after washing in (f), where fermentable sugars are produced; and fermenting the sugars to produce a target product, where the target product is selected from alcohols, organic acids, amino acids and gases. The biomass is selected from switchgrass, waste paper, sludge from paper manufacture, corn fiber, corn cobs, corn husks, corn stover, grasses, wheat, wheat straw, hay, barley, barley straw, rice straw, sugar cane bagasse, sugar cane straw, yellow poplar, sorghum, soy, components obtained from processing of grains, trees, branches, roots, leaves, wood chips, sawdust, shrubs and bushes, vegetables, fruits, flowers, animal manure and combinations. In simultaneous fragmentation and selective extraction of lignin from lignocellulosic biomass to produce a substantially lignin-free biomass, the substantially lignin-free biomass is 60-100% original weight of the biomass. The organic solvent solution further comprises one or additional component selected from alkali or alkaline earth hydroxides or carbonates, ammonia, thiols, polysulfides, hydropolysulfides and hydrosulfides, sulfides and combinations. The solvent solution, and any unreacted elemental sulfur or other unreacted components are recyclable. The organic solvent solution comprises a solvent selected from alcohols, diols and aprotic solvents, where the organic solvent solution comprises a solvent selected from methanol, ethanol, propanol, butanol, pentanol and hexanol, isomers, and diols. The lignocellulosic biomass of (a) has a carbohydrate content that is highly conserved through (a)-(d). The alkylamines is selected from R-NH2, R2-NH, R3N, (H2N-R-NH2), (H2N-R(NH2)2), (HO-R-NH2), ((HO)2-R-NH2), (HO-R-(NH2)2), (HS-R-NH2), ((HS)2-R-NH2), (HS-R-(NH2)2) and (H2N-R(OH)(SH)) and combinations.R=a monovalent, divalent or trivalent 1-6C alkane, alkene or alkyne, linear, cyclic or branched; or methyl, ethyl, propyl or butyl.Moreover, the alkylamine is methylamine. The temperature of (c) is 165-195 degrees C. EXAMPLE - No suitable example given.