TECHNOLOGY FOCUS - FOOD - Preferred Method: The first component comprises 10-30 (preferably 13-17) wt.% solids during the step of treating the first component into the liquefied first component. The first enzyme formulation is supplied at a concentration of 1-20 mg of enzyme protein/g of glucan during the step of treating the first component into the liquefied first component. The pH and the temperature of first component is maintained at 4-6 and at 30-60 degrees C, respectively during the step of treating the first component into the liquefied first component. The retention time of the first component is 1-30 hours during the step of treating the first component into the liquefied first component. The viscosity of the first component is reduced during the step of treating the first component into the liquefied first component. The pre-treating of the biomass comprises utilizing an acid pre-treatment system. The second enzyme formulation and the ethanologen are supplied to the liquefied first component simultaneously. The supply of the second enzyme formulation is initiated before the supply of ethanologen into the liquefied first component. The glucan comprises cellulose and the first enzyme formulation is capable of liquefying glucan. Preferred Components: The first component comprises glucan and lignin. The fermentation product comprises lignin. The liquefied first component comprises liquefied biomass to be supplied to the fermentation system. The first component comprises a solids component of the lignocellulosic material. The second component comprises a liquid component of the lignocellulosic material.    TECHNOLOGY FOCUS - INSTRUMENTATION AND TESTING - Preferred Components: The biorefinery further comprises a second vessel for saccharification and fermentation of the liquefied first component. The first treatment system comprises a vessel for liquefaction of the first component. The vessel is a continuously stirred reactor (CSTR). The system for pre-treating the biomass comprises an acid pre-treatment system.