NOVELTY - Preparation of special biofertilizer comprises screening mixture of at least one of peat, diatomite, montmorillonite, lignite, coal ash, bentonite and zeolite; respectively fermenting different microorganism; respectively absorbing fermented microorganism liquids in screened mixture; uniformly mixing single microorganism preparation after absorption to prepare biological agent; mixing cake fertilizers; and mixing biological agent, cake fertilizer mixture, silkworm excrement, chitosan, attapulgite powder, humic acid, and livestock and poultry excrement.    USE - Preparation of special biofertilizer of tobacco.    ADVANTAGE - The method has easily-obtained raw materials; has low production cost and stable performance, provides lasting fertilizer effect and strong stress resistance; increases yield; and improves product quality.    DETAILED DESCRIPTION - Preparation of special biofertilizer comprises uniformly mixing at least one of peat, diatomite, montmorillonite, lignite, coal ash, bentonite and zeolite; screening mixture through 0.1-2 cm screen to obtain first sample; respectively fermenting Anabaena azotica, Chlorella vulgaris, Chlamydomonas sajao, Bacillus subtilis, Bacillus mucilaginosus and Streptomyces jingyangensis; respectively absorbing fermented microorganism liquids with 2-10 times by weight of first sample; uniformly mixing single microorganism preparation after absorption according to ratio of 15-35:5-55:0-25:10-30: 0-35:10-50 to obtain second sample; uniformly mixing at least one of bean cake, rapeseed cake, pork cake, cotton seed cake, peanut cake, tung tree seed cake, and Camellia oleosa seed cake with water content of 5-30% with silkworm excrement, chitosan and attapulgite powder according to ratio of 35-75:5-50:0-10:0-10 to obtain third sample; and mixing (%) second sample (70-90), third sample (5-25), humic acid (3-3.5), and pig excrement, cow excrement, and/or chicken excrement (0-5.5) with water content of 5-30 wt.% to obtain final product.