NOVELTY - Processing (M1) lignocellulosic biomass involves subjecting the fiber fraction to an initial enzymatic hydrolysis using an initial cellulase enzyme dose of greater than 15 filter paper units (FPU)/g dry solid matter (DM) to a conversion of greater than or equal to 95%, followed by subsequent hydrolysis cycles where cellulase activity recovered from one hydrolysis mixture is used along with a fresh cellulase supplementation dose of 3-8 FPU/g DM to hydrolyse additional fiber fraction in hydrolysis mixture, where the amount of cellulase activity recovered from one hydrolysis mixture is on average at least 58%.    USE - For processing lignocellulosic biomass (claimed) such as for processing of lignocellulosic biomass in bioethanol production. The lignocellulosic biomass includes wheat straw, rice straw, bagasse, grasses, corn stover, or empty fruit bunches.    ADVANTAGE - The method provides rapid and low cost enzymatic full conversion of lignocellulosic biomass. The method enables improved processing of lignocellulosic biomass in bioethanol production. The used cellulase enzymes are subsequently recycled and used in subsequent hydrolysis cycles along with a lower dose supplementation of fresh enzyme. Loss of enzyme activity between hydrolysis cycles is offset by improved overall process advantage. The high enzyme dose in the method provides overall advantage even where consumption of enzyme per liter ethanol is increased relative to a low dose system. The use of a high dose regime results in dramatic reduction of hydrolysis times. This reduction in hydrolysis times provides direct benefits in production scale in that capital costs are reduced (smaller hydrolysis tanks can be used) and production capacity increased (higher biomass throughput is achieved). Further, in the high dose regime, lignocellulosic biomass is more completely hydrolyzed such that conversion approaches 100%. This in turn reduces overall biomass costs per liter ethanol. In other cases, hydrolysis yields can be so significantly improved and cellulase activity recycled to so high degree that the high dose regime provides equivalent or even lower final enzyme consumption per liter ethanol than can be achieved using lower enzyme doses recommended by commercial enzyme suppliers.    DETAILED DESCRIPTION - Processing (M1) lignocellulosic biomass involves providing fiber fraction from hydrothermally pretreated lignocellulosic biomass; subjecting the fiber fraction to an initial enzymatic hydrolysis using an initial cellulase enzyme dose of greater than 15 filter paper units (FPU)/g dry solid matter (DM) to a conversion of greater than or equal to 95%, followed by subsequent hydrolysis cycles where cellulase activity recovered from one hydrolysis mixture is used along with a fresh cellulase supplementation dose of 3-8 FPU/g DM to hydrolyse additional fiber fraction in a subsequent hydrolysis mixture, where the amount of cellulase activity recovered from one hydrolysis mixture and reused in a subsequent hydrolysis mixture is on average at least 58% of the amount present at the start of the hydrolysis from which activity is recovered, and where the cycle of enzyme recovery and supplementation with fresh cellulase in a subsequent hydrolysis mixture is repeated at least three times. An INDEPENDENT CLAIM is included for method (M2) of recovering cellulase activity bound to hydrolysis residual involving washing hydrolysis residual at pH 9, or 7-9, where the recovered cellulase preparation is inactive in the pH range used and where greater than or equal to 40% of bound cellulase enzyme is recovered in the wash.