TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Components: The concentration of the aqueous liquid in the suspension is above 10 mass%, preferably 90 mass%. The lipids includes: one or more triglycerides; and hydrophilic moieties, and the exterior layers result from an exterior configuration of the hydrophilic moieties. The exterior layers include cell walls. The aqueous liquid includes natural or synthetic seawater. The nonpolar solvent includes an alkane. The alkane includes hexane. The particles include cells, and disrupting includes lysing the cells. The first product includes a larger portion of the nonpolar solvent than does the second product. The segregated mixture further comprises a third product having a majority of one or more non-lipid biomass substances. The particles include algae and diatoms. The second product includes a majority of the aqueous liquid. The segregating includes segregating the mixture into first, second, and third products. The first product includes a majority of the lipids, the second product includes a majority of a non-lipid biomass, and the third product includes a majority of the aqueous liquid. The non-lipid biomass includes one or more proteins and one or more carbohydrates. The extracted substance includes a lipid, and the biofuel is synthesized from the lipid using a process that includes transesterification of the lipid. The membrane or filter has a mean pore size of 1 nm to 1 mm or 1-100 mu . The system further comprises a separation station configured to remove at least one of the segregation products. Preferred Method: The disrupting includes: agitating the suspension or mixture; and heating the mixture. The agitating includes stirring and ultrasonicating. In the method, at least a portion of the algae includes a member of the genus Nannochloropsis. The heating includes heating to above 80 degrees C or 150 degrees C. The heating comprises heating the mixture to a temperature above a boiling point at ambient pressure of the nonpolar solvent or the aqueous liquid. The method further comprises: controlling a pressure above the heated mixture to a value that prevents boiling of the nonpolar solvent and the aqueous liquid; and adjusting the chemistry of any of the suspension, mixture, and products. The controlled pressure is below 500 psig or 200 psig. The segregating includes: allowing the mixture to settle according to gravity; and centrifuging. The centrifuging induces an acceleration that does not exceed 500 or 3000 times the force of gravity. The centrifuging induces an acceleration of 5-50000 (preferably 10-10000) times the force of gravity. The forcing includes applying a pressure: that does not exceed 30 bar to the mixture; and of 5-100 bar to the mixture. The adjusting the chemistry includes: adjusting pH; adding one or more acids; adding one or more bases; and dissolving a chemical species from a gas phase passed through any of the suspension, mixture, and products. The disruption station includes a pressure vessel, and may be heated at above 30 degrees C. The segregation station includes a centrifuge. The segregation station includes: a membrane or filter that is permeable to a first substance in the disrupted suspension and substantially impermeable to a second substance in the disrupted suspension; and a pressure vessel configured to force the first substance through the membrane. The segregating includes forcing the mixture through a membrane or filter that is permeable to at least a portion of one of the first and second products and substantially impermeable to at least a portion of the other of the first and second products.