TECHNOLOGY FOCUS - BIOLOGY - Preferred Components: The microorganism is fungus. The fungus is filamentous fungus, white rot fungus, Aspergillus fumigatus or yeast. The white rot fungus is Phanerochaete chrysosporium. The microorganism is bacterium or protist. The protist is algae. The parasite is nematode. The blood is abattoir blood. The animal is human.    TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Processes: The method further comprises separately collecting at least a portion of one or both of the layer comprising organic solvent and the layer comprising the aqueous solution; fermenting fermentable sugar to produce alcohol, the fermentable sugar in the layer comprising aqueous solution; sterilizing biodegradable substrate; increasing surface area of the biodegradable substrate; optimizing the physiochemical conditions for culture of the microorganism; isolating at least a portion of the lipids from the organic solvent; recovering organic solvent, reusing recovered organic solvent, producing biodiesel from lipids; isolating glucose from aqueous layer, producing 1-12 from the glucose, storing hydrogen (H2) in a container; using H for fuel and using CO2 to culture microorganism; fermenting fermentable sugar to produce an alcohol; isolating alcohol; producing a nutrition supplement from the amino acids; producing a sweetener from the polyhydric alcohol; and producing detergent from enzyme; producing a nutrition supplement from vitamin, isolating at least a portion of the non-lipid materials, using at least a portion of the aqueous layer as nutritional supplement; purifying fresh or pure water from layer comprising aqueous solution; floating biomass, lysate and/or autolysate inlet unit on the layer comprising the organic solvent, where the biomass, lysate and/or autolysate inlet unit delivers the biomass, lysate and/or autolysate; floating organic solvent inlet unit on the layer comprising the organic solvent, where the organic solvent inlet unit comprises the first port; and floating outlet unit with the second port on the organic solvent layer while the organic solvent comprising lipids is drawn. The sterilizing comprises adding chlorine dioxide. The increasing comprises mechanical process of grinding, chopping and crushing. The optimizing comprises supplying nutritional supplement or supplying gas, and modulating temperature, humidity or pH. The harvesting comprises mechanically collecting microorganism, or collecting a liquid solution comprising the microorganism. The mechanically collecting comprises manual collection, automated collection or centrifugation. The inducing autolysis comprises mechanical process involving homogenization, chemical process, sonicating microorganism, enzymatic process, modulating temperature, modulating amount of light, or starving microorganism biomass. The chemical process comprises adding a chemical compound comprising chlorine. The modulating comprises increasing or decreasing temperature. The modulating comprises increasing or decreasing the amount of light. The allowing the microorganism biomass to undergo autolysis occurs for 1-120 hours (preferably 1-60 minutes). The step of allowing the microorganism to undergo autolysis further comprises inhibiting cellular glucose oxidase. The inhibiting cellular glucose oxidase comprises adding hydrogen peroxide to autolysate. The storing autolysate is at less than 10 (preferably 2-3) degrees C. The separately collecting comprises decanting or skimming. The recovering comprises evaporation and condensation. The producing biodiesel comprises transesterification of lipids. The transesterification utilizes batch reactor, supercritical alcohol, ultrasonic reactor or microwave irradiation. The isolating comprises chromatography. The production comprises utilizing synthetic enzymatic pathway that yields H2 and carbon dioxide (CO2) from glucose. The fermentation utilizes Saccharomyces cerevisiae, Zymomonas mobilis, Clostridium beijerinckii, C. acetobutylicum or C. tetanomorphum, and produces CO2 that can be used to culture microorganisms. The isolation utilizes steam stripping distillation, adsorption, gas stripping or pervaporation. The fermentable sugar produces acetone. The nutrition supplement is for animal consumption, or a plant fertilizer. The isolating utilizes electrophoresis, chromatography, membrane separation or utilizes centrifugation. The nutritional supplement is used for consumption an animal or microorganism. The pure water is for agricultural use, and for drinking by animals. The processing biomass further comprises mixing layer comprising aqueous solution by moving mixing elements while the biomass, lysate and/or autolysate inlet unit, the organic solvent inlet unit, and the outlet unit float on the layer comprising the organic solvent; delivering material from layer comprising the aqueous solution to a collection unit; and separating component(s) of the delivered material from the layer comprising the aqueous solution using the collection unit. Preferred Components: The biodegradable substrate is solid substrate or liquid substrate. The solid substrate comprises agricultural waste, landscaping waste, solid municipal waste, calcium carbonate, coal, animate, or parasite. The liquid substrate comprises liquid municipal waste, blood, crude oil, salt water, or fresh water. The gas comprises carbon dioxide (CO2) or oxygen (O2). The organic solvent is hexane. The glycerol is byproduct of transesterification. The aqueous layer comprises glucose. The aqueous layer comprises fermentable sugar. The fermentable sugar comprises glucose, fructose, or sucrose. The alcohol is ethanol, butanol or isopropanol. The aqueous layer comprises amino acids. The aqueous layer comprises organic acid, inorganic acid, polyhydric alcohol, nitrogenous compound, enzyme, or vitamin. Preferred Content: The biodiesel and glycerol are produced at ratio of 9:1.