NOVELTY - Process microorganism biomass comprises inducing autolysis of microorganism biomass; and allowing microorganism biomass to undergo autolysis in presence of organic solvent and aqueous solution, and producing autolysate comprising layer comprising organic solvent and layer comprising aqueous solution. The layer comprises organic solvent comprising lipids released from microorganism biomass during autolysis, and layer comprising aqueous solution comprising non-lipid materials released from microorganism biomass during autolysis.    USE - Method of processing microorganism biomass used for producing lipid and non-lipid materials (claimed).    ADVANTAGE - The method uses biodegradable material and microorganism, eliminates the use of heat and acids for starch hydrolysis by utilizing autolysis instead, does not need high temperature, acid treatment, and/or high pressure, and is low cost.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for:    (1) production of lipids and non-lipid materials comprising culturing microorganism on biodegradable substrate to produce microorganism biomass, harvesting microorganism biomass, inducing autolysis of microorganism biomass, and allowing microorganism biomass to undergo autolysis in the presence of an organic solvent and aqueous solution, producing an autolysate comprising layer comprising organic solvent and layer comprising an aqueous solution, and separately collecting at least a portion of one or both of the layer comprising the organic solvent and the layer comprising the aqueous solution;    (2) a method of producing fresh water comprising culturing a microorganism on biodegradable substrate comprising salt or brackish water to produce a microorganism biomass, harvesting microorganism biomass, inducing autolysis of microorganism biomass, allowing microorganism biomass to undergo autolysis in presence of an organic solvent and aqueous solution, and collecting layer comprising aqueous solution;    (3) a method of producing pure water comprising culturing microorganism on a biodegradable substrate comprising contaminated water, harvesting microorganism biomass, inducing autolysis of the microorganism biomass, allowing microorganism biomass to undergo autolysis in presence of an organic solvent and an aqueous solution, and collecting layer comprising the aqueous solution;    (4) a processing system comprising container having a chamber for holding biomass and autolysate, mixing apparatus, feed apparatus including biomass, lysate, and/or autolysate source coupled to the container and an organic solvent source coupled to the container, collection unit in fluid communication with container, and controller communicatively coupled to the feed apparatus and the collection unit;and    (5) a method of processing biomass comprising delivering biomass, lysate and/or autolysate in an aqueous solution to processing system, delivering organic solvent out of a first port of processing system, allowing aqueous solution of the layer comprising the aqueous solution to ferment, and drawing organic solvent comprising lipids from the layer comprising the organic solvent into a second port positioned within the layer comprising the organic solvent while the aqueous solution ferments.