NOVELTY - Producing high intensity heterotrophic algae comprises: (i) cultivating micro algae by improving cell intensity, (ii) increasing the promotion of cell volume and fatty acid accumulation for micro algae cultivation, (iii) cultivating the algae by promoting increase in fatty acid under different conditions.    USE - The method is useful for producing high intensity heterotrophic algae.    ADVANTAGE - The method: changes all the variables, e.g. carbon source, type and concentration of nutritive factor, oxygen dissolution quantity and temperature during the growth of the alga in three stages to induce maximal biomass, lipid and DHA and hence the cell activity of majority cell is subjected to oriented cracking to form many cells quickly; and greatly improves the yielding ratio of the final biomass and the lipid.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for:    (1) producing lipid, comprising: (a) cultivation of micro algae in a cultivating medium for promoting the cell intensity to at least 800000000 cells per ml, (b) increasing the promotion of cell volume and lipid accumulation for micro algae cultivation, (c) cultivating the algae by promoting increase in lipid accumulation;    (2) producing bio-diesel and fatty acid by addition of microorganism, comprising: producing a byproduct of crude glycerol by pre-processing the crude glycerol to form glycerol, which is used as carbon source for cultivation and production of the lipid microorganism to produce the algae biomass rich in fatty acid, processing the algae to obtain omega -3 fatty acid and non- omega -3 fatty acid, separating the omega -3 fatty acid from the non- omega -3 fatty acid, where the production of biological fuel utilizes non- omega -3 fatty acid and produces extra crude thus integrating the bio diesel and the fatty acid production; and    (3) integrating bio diesel and producing omega -3 fatty acid, comprising: (p) refining the bio diesel to obtain crude glycerol, (q) using the crude glycerol as the carbon source to produce the algae biomass rich in fatty acid, comprising omega -3 fatty acid and non- omega -3 fatty acid, (r) collecting omega -3 fatty acid, and (s) using non- omega -3 fatty acid to produce bio diesel and crude glycerol, and (t) repeating the steps (q) to (s).