TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Components: At least one of the Tri5, Tri6, or Tri10 nucleic acid sequences has been modified in order to give the gene constitutive activity in producing a gene product. The mutant filamentous fungus (F1) is selected from: Acremonium, Aspergillus, Aureobasidium, Cryptococcus, Filibasidium, Fusarium, Gibberella, Humicola, Magnaporthe, Mucor, Myceliophthora, Myrothecium, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Piromyces, Stachybotrys, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Thchoderma, or Trichothecium strain (preferably Fusarium, especially Fusarium sporotrichioides (NRRL 3299)). At least two of the nucleic acid sequences from Tri5, Tri6 and Tri10, have been modified. There is a nonrevertable site-selected deletion of part or all of nucleic acid coding for the Tri4 such that the Tri4 gene is inactivated. There is a nonrevertable site-selected deletion or modification to the nucleic acid sequence encoding for the Tri4 gene has been modified such that the Tri4 gene product enzymatic or catalytic activity is reduced by at least 10% when compared to the parent strain under the same conditions. The modified sequence of (b) comprises more than one copy of the nucleic acid sequence encoding for at least one of the genes selected from Tri5, Tri6 and Tri10. At least one of the additional copies of the nucleic acid sequence encoding for at least one of the genes Tri5, Tri6 and Tri10, is in a vector which is capable of autonomous maintenance in the filamentous fungus. The modified sequence of (b) comprises coding for the gene operably linked to a promoter from a constitutively active filamentous fungal gene. The Tri5, Tri6 or Tri10 gene is modified to increase the conversion of farnesyl pyrophosphate (FPP) to trichodiene. The Tri6 and/or Tri10 gene is modified to at least reduce the production of the Tri4 gene product. Preferred Process: In the process (M1): the biomass growth media is selected from: pectin, galacturonic acid, sugar, starch, cellulose, or hemicelluloses. The process is conducted at the production source of the growth media culture. The filamentous fungus produces at least 0.25 g of trichodiene per gram of glucose or glucose equivalent consumed. The media culture includes a lignocellulosic feedstock. EXAMPLE - No suitable example is given.