NOVELTY - Treatment of methyl orange dye wastewater comprises inoculating Acetobacter xylinum CGMCC 1.1812 into seed culture medium, adding modified bacterial cellulose membrane immobilizing panerochaete chrysosporium (1-2 g) into methyl orange dye wastewater (50 ml), treating for 5 days on gas-bath thermostatic table concentrator at 30 degrees C and 120 revolutions/minute, measuring chrominance of wastewater every 24 hours, 5 days after, removing treated wastewater, reserving immobilized fungus, and adding prepared dye wastewater (50 ml).    USE - Method for treating methyl orange dye wastewater (claimed).    DETAILED DESCRIPTION - Treatment of methyl orange dye wastewater comprises: (1) inoculating Acetobacter xylinum CGMCC 1.1812 into seed culture medium (50 ml) comprising (%) glucose (2), peptone (0.5), yeast cream (0.3), sodium phosphate (0.2), potassium phosphate (0.1), magnesium sulfate (0.025) and citric acid (0.02) with pH 5.8, culturing 2 days on gas-bath thermostatic table concentrator at 160 revolutions/minute to obtain activated acetobacter xylinum culture solution; inoculating culture solution (10 ml) into fermentation medium (125 ml) comprising (%) glucose (2), peptone (0.5), yeast cream (0.3), sodium phosphate dibasic dehydrate (0.2), monopotassium phosphate (KH2PO4) (0.1), magnesium sulfate heptahydrate (MgSO4.7H2O) (0.025) and citric acid (0.02), pH at 5.8, standing and culturing for 7 days at 30 degrees C until generating light yellow colloid membrane on liquid level, taking out membrane, washing repeatedly by distilled water to remove culture medium and impurities on surface of the membrane, soaking membrane in 0.1 M sodium hydroxide (NaOH) solution, boiling for 60 minutes at 80 degrees C to remove thallus and residual culture medium in membrane, and washing repeatedly by distilled water until neutral to obtain bacterial cellulose membrane; (2) adding bacterial cellulose membrane (20-30 g) to 1 M NaOH solution (100-150 ml), adding epoxy chloropropane (9-12 ml) to react for 60-90 minutes at 50 degrees C of thermostatic water bath, taking out of membrane, washing by acetone, adding ethylenediamine (5-10 ml) and distilled water (95-190 ml), shaking, heating for 20-30 minutes at 70 degrees C of thermostatic water bath, taking out of membrane, washing by distilled water to neutralize, washing for 3 times by 1% hydrochloric acid, washing by distilled water to neutralize, moisturizing by acetone, drying in vacuum to constant weight to obtain modified bacterial cellulose membrane; (3) adding modified bacterial cellulose membrane (3-5 g) into 0.2 M phosphate buffer (20-30 ml) and 7 pH, adding glutaraldehyde water solution (2-3 ml), stirring gently at 35 degrees c for 60-90 minutes, taking out of membrane, washing by distilled water and 0.2 M phosphate buffer having 7 pH, drying in vacuum, adding dried bacterial cellulose membrane into phanerochaete chrysosporium liquid restrictive medium (80-100 ml) comprising (g/l) KH2PO4 (2), MgSO4.7H2O (0.25), manganese sulfate (0.5 mg/L), absolute iron sulfate (0.1), sodium chloride (1 mg/L), ammonium nitrate (2), calcium chloride (0.1), glucose (15) and Vitamin B1 (5 mg/L), pH of 6-7, sterilizing and adding phanerochaete chrysosporium spore liquid (8-10 ml) to culture for 48 hours on table concentrator at 30 degrees C under 120 revolutions/minute, taking out of membrane, washing for 3 times by sterile water, and forming a layer of thin white fungus membrane on the surface of membrane, i.e. modified bacterial cellulose membrane immobilizing panerochaete chrysosporium; (4) adding modified bacterial cellulose membrane immobilizing panerochaete chrysosporium (1-2 g) into methyl orange dye wastewater (50 ml) prepared by weighing methyl orange (80-160 mg/L), dissolving in 1 L phanerochaete chrysosporium liquid restrictive medium to obtain methyl orange dye wastewater, treating for 5 days on gas-bath thermostatic table concentrator at 30 degrees C and 120 revolutions/minute, measuring chrominance of wastewater every 24 hours, 5 days after, removing treated wastewater, reserving immobilized fungus, adding prepared dye wastewater (50 ml), treating, measuring chrominance of wastewater every 24 hours, taking 5 days as circulation, repeating for 5 times, and finishing with removal rate of chrominance of wastewater of more than 81%.