TECHNOLOGY FOCUS - INORGANIC CHEMISTRY - Preferred Components: The acid is selected from hydrochloric acid, nitric acid, phosphoric acid, sulfuric acid, boric acid, hydrofluoric acid, hydrobromic acid and/or carbon dioxide. The base is selected from sodium hydroxide, potassium hydroxide, calcium hydroxide, and other metal hydroxides from the alkali metals and alkaline earth metals, ammonium hydroxide, ammonia, sodium carbonate, potassium carbonate, boron hydroxide, aluminum hydroxide, borax, amino alcohols such as ethanol amine, diethanolamine, triethanolamine, isopropanolamine, diisopropylamine, triisopropylamine, propylamine, 2-propylamine, methylamine, dimethylamine, trimethylamine, dimethylethanol amine, monomethylethanolamine, 2-(2-aminoethoxy)ethanol, diglycolamines and/or diethylamine and other similar polyamines.    TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Components: The acid is selected from acetic acid, lactic acid, formic acid and/or propionic acid. The polar solvent is a combination of water and at least one polar solvent selected from low molecular weight aldehydes, ketones, fatty acids, methanol, ethanol, propanol, butanol, formic acid, acetic acid, propionic acid and amphipathic solvents. The non-polar solvent is selected from carbon dioxide, carbon tetrachloride, chloroform, cyclohexane, 1,2-dichloroethane, dichloromethane, diethyl ether, dimethyl formamide, ethyl acetate, propane, butane isomers, pentane isomers, hexane isomers, heptane isomers, octane isomers, nonane isomers, decane isomers, methyl-tert-butyl ether, toluene, butene isomers, pentene isomers, hexane isomers, heptene isomers, octene isomers, nonene isomers, decene isomers, mineral spirits, and 2,2,4-trimethyl pentane.    TECHNOLOGY FOCUS - PHARMACEUTICALS - Preferred Method: The permeability conditioning step is further defined as adding an acid to the biomass, where the pH is adjusted to 1.0 to 6.5; or as adding a base to the biomass, where the pH is adjusted to 7.5 to 14. The permeability conditioning step further includes the step of heating the biomass to a temperature of 25-200 degrees C, of 45-150 degrees C, of 55-140 degrees C and of 60-130 degrees C. The permeability conditioning step further includes a disordering treatment selected from mechanical treatment, electrical treatment, osmotic shock, infection with a lytic virus, exposure to elevated pressure, rapid pressure oscillation conditions and/or vacuum and pressure oscillation. The permeability conditioning step further includes the step of subjecting the biomass to low voltage pulse electric fields selected from voltage 1 to 150 volts, and voltage 150 to 9000 volts. The permeability conditioning step further includes addition of enzymes in conjunction with acid or in replacement of acid. The method further includes the step of repeat extraction utilizing alcohol post fermentation for additional lipid fractionation or esterification of fatty acids. When the polar solvent in addition to water is an alcohol selected from amyl alcohols, propanols, and butanols, the method further includes the step of simultaneously trans-esterifying acylglycerols and esterifying free fatty acids. The partitioning step is further defined as separating the non-polar solvent solution from the polar biomass solution and residual biomass by a process selected from centrifugation, coalesce, decantation, variation in pressure, high flux membranes, ultrasonification, heating and adding to the multi-phase suspension, an oil-water de-emulsifying agent; performed at a temperature range selected from 25-200 degrees C, 55-180 degrees C and 70-170 degrees C. The method of fractionating biomass includes the steps of permeability conditioning biomass suspended in a water solution of carbon dioxide to form a conditioned biomass; partitioning to obtain a non-polar solvent solution and a polar biomass solution; recovering cell products from the non-polar solvent solution and polar biomass solution; and fractionating polar and water soluble components from the biomass, where the carbon dioxide is selected from liquid carbon dioxide and supercritical carbon dioxide. The permeability conditioning step further includes enzymatic hydrolysis using enzymes such as among others, endoglucanases, exoglucanases, cellobiohydrolases, beta -glucosidase, endoxylanases, beta -xylosidases, beta -mannosidase, galactomannan-degrading enzymes, pectin lyases, pectate lyases, endopolygalacturonase-II, arabinofuranosidases, arabinoxylan, arabinofuranohydrolases, alpha -glucuronidases, feruloyl and para-coumaroyl esterases, cellulases, lipases, hemicellulase, pectinase and algaenan. EXAMPLE - No suitable example given.