NOVELTY - Improving bio-hydrogen production yield, comprises: (a) adding sludge into a taper bottle using a set of laboratory reaction devices, adding a mixed liquid as a nutrient source, where the mixed liquid is obtained by mixing primary compost leachate, glucose and fruit juice waste water; (b) sealing obtained mixed liquid, and placing in a constant-temperature water bath oscillator, discharging hydrogen and gradually passing into a collecting bottle, calculating the yield of hydrogen; and (c) exploring optimal adding ratio of leachate and glucose and proceeding verification through experiment.    USE - The method is useful for improving bio-hydrogen production yield.    ADVANTAGE - The method: verifies feasibility of hydrogen production under warm conditions and leachate as an external nutrition source, and predicts by response surface model that glucose of 6174.93 mg /l and leachate organic load of 3883.20 mg chemical oxygen demand/l, obtains a highest molecular hydrogen yield (1.60 mol hydrogen/mol of glucose) and a highest accumulated hydrogen volume (469.74 ml); and reliable as verification experiment value is substantially similar to predicated value of the corresponding curved surface, and accounts on an average 44% and 39% of total volatile fatty acid.    DETAILED DESCRIPTION - Improving bio-hydrogen production yield, comprises: (a) adding sludge into a taper bottle (500 ml) using a set of laboratory reaction devices, where the sludge contains 0.28-2 mm of black granules, adding a mixed liquid as a nutrient source, where the mixed liquid is obtained by mixing primary compost leachate, glucose and fruit juice waste water; (b) sealing the obtained mixed liquid in the cone-shaped bottle, and placing in a constant-temperature water bath oscillator for anaerobic fermentation to produce hydrogen, discharging hydrogen from an exhaust pipe above liquid level, gradually passing into a collecting bottle filled with water, where gas is in water filled collecting bottle is collected and discharged into a gas collecting bottle, calculating the yield of hydrogen based on the liquid level scale displayed in the collecting bottle, where the constant temperature water bath oscillator maintains stability to 35 plus minus 1 degrees C and initial pH is controlled to 5.5; and (c) using the response curve surface optimization design, exploring optimal ratio of leachate and glucose in the mixed liquid, and proceeding verification through experiment.