TECHNOLOGY FOCUS - BIOLOGY - Preferred Method: The disruption of the fatty acid metabolism pathway further comprises contacting the plant cell or fungus with a glycolytic inhibitor. The fatty acid metabolism pathway is gluconeogenesis or fatty acid oxidation. The syngas is processed by a Fischer-Tropsch reaction to produce a biodiesel. The biodiesel is processed using a transesterification process. The transesterification process is achieved by mixing the fatty acids with methanol. The fatty acids are processed to produce biofuel using a thermochemical liquefaction process or pyrolysis process. Preferred Components: The inhibitor of fatty acid metabolism: is an inhibitor of fatty acid oxidation (preferred), fatty acid transporter inhibitor, reductase inhibitor or isomerase inhibitor within the fatty acid metabolism pathway; is an inhibitory nucleic acid; gluconeogenesis inhibitor i.e. oxamate; or inhibitor of uncoupling protein. The inhibitory nucleic acid is specific for an enzyme comprising 2,4-dienoyl-coenzyme A (CoA) reductase, 2,4-dienoyl-CoA isomerase or butyryl dehydrogenase. The plant or fungus is an alga, a plant seed or Schizochytrium. The RNAi construct is short hairpin RNA or micro RNA. The algae further comprise stably-integrated polynucleotide encoding an acetyl CoA carboxylase gene. The plant cell further comprises stably-integrated polynucleotide encoding an acetyl CoA carboxylase gene.    TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Components: The inhibitor of fatty acid metabolism is oxirane carboxylic acid compound e.g. etomoxir (2-(6-(4-chlorophenoxy)-hexyl)-oxirane-2-carboxylic acid ethyl ester), 2-(4-(3-chlorophenoxy)-butyl)-oxirane-2-carboxylic acid ethyl ester, 2-(4-(3-trifluoromethylphenoxy)-butyl)-oxirane-2-carboxylic acid ethyl ester, 2-(5(4-chlorophenoxy)-pentyl)-oxirane-2-carboxylic acid ethyl ester, 2-(6-(3,4-dichlorophenoxy)-hexyl)-oxirane-2-carboxylic acid ethyl ester, 2-(6-(4-fluorophenoxy)-hexyl)-oxirane-2-carboxylic acid ethyl ester, 2-(6-phenoxyhexyl)-oxirane-2-carboxylic acid ethyl ester, cerulenin, 5-(tetradecyloxy)-2-furoic acid, oxfenicine, methyl palmoxirate, metoprolol, amiodarone, perhexiline, aminocarnitine, hydrazonopropionic acid, 4-bromocrotonic acid, trimetazidine, ranolazine, hypoglycin, dichloroacetate, methylene cyclopropyl acetic acid, beta -hydroxy butyrate or non-hydrolyzable analog of carnitine or their salts. The biofuel is syngas i.e. biodiesel. The glycolytic inhibitor is 2-deoxyglucose compound. EXAMPLE - No suitable example given.