NOVELTY - An isolated nucleic acid encoding a polypeptide having at substantial homology to at least one of the 70 defined sequences comprising 252-1217 amino acids (SEQ ID NO: 1-70), given in the specification, where the nucleic acid encodes at least one polypeptide having enzyme activity, is new.    USE - The nucleic acid, and expression cassette, vector, or cloning vehicle are useful for cost-effective processing of lignocellulosic biomass, producing fermentable sugars, fractionating corn grain into a high starch portion and a high fiber-containing bran portion, reducing recalcitrance of plant biomass to pretreatment and hydrolysis (all claimed), and for biomass conversion, biofuel production, enhanced forage digestibility by animals, improving fiber qualities, and enhancing fiber processing properties.    DETAILED DESCRIPTION - An isolated nucleic acid encoding a polypeptide having at substantial homology to at least one of the sequences selected from SEQ ID NO: 1-70, where the nucleic acid encodes at least one polypeptide having enzyme activity selected from feruloyl esterase, xylanase, alpha-L-arabinofuranosidase, endogalactanase, acetylxylan esterase, beta-xylosidase, xyloglucanase, glucuronoyl esterase, endo-1,5-alpha-L-arabinosidase, pectin methylesterase, endopolygalacturonase, exopolygalacturonase, pectin lyase, pectatelyase, rhamnogalacturonanlyase, pectin acetylesterase, alpha-L-rhamnosidase, mannanaseexoglucanase, cellulase, licheninase, laminarinase, beta-(1,3)-(1,4)-glucanase, beta-glucosidase activity, and their combinations. INDEPENDENT CLAIMS are:    (1) an expression cassette, vector, or cloning vehicle comprising a nucleic acid;    (2) a transformed cell comprising a nucleic acid or an expression cassette, a vector, or a cloning vehicle of (1), where the cell is a plant cell;    (3) a transgenic plant, or plant part, comprising a sequence or an expression cassette, a vector or a cloning vehicle, or a transgenic plant, or plant part, comprising more than one nucleic acid or more than one expression vector, where the plant is created either by gene stacking technology or by traditional plant breeding method;    (4) a method for cost-effective processing of lignocellulosic biomass by pretreatment of a plant part under conditions that promote hydrolysis of cellulose and hemicellulose to fermentable sugars, where the plant part is obtained from at least one transgenic plant of (3);    (5) an expression cassette, vector, or cloning vehicle comprising a nucleic acid comprising a gene encoding an enzyme selected from a cellulase, hemicellulase accessory enzyme, and their combinations, where the expression cassette, vector, or cloning vehicle is capable of being introduced into plant cell;    (6) a transgenic grain crop plant comprising an expression cassette, vector, or cloning vehicle of (5), where the enzyme or combination of enzymes is expressed in the grain;    (7) a method of producing fermentable sugars by providing grain from the transgenic plant of (6) and hydrolyzing both starch and fiber in the grain simultaneously after the grain is ground, or hydrolyzing the starch portion with amylase enzymes;    (8) a method of fractionating corn grain into a high starch portion and a high fiber-containing bran portion by obtaining corn kernel from the transgenic plant of (6) and separating the corn kernel into a high starch portion and high bran portion;    (9) a method of producing higher levels of fermentable sugars compared to non-transgenic corn grain of (6) by hydrolyzing the cellulose and hemicellulose present in the bran portion with endogenous cellulase or hemicellulase;    (10) a transgenic maize plant comprising an expression vector that produces cellulase or hemicellulase enzyme in the corn grain and containing lysine at levels greater than that of non-transgenic maize plants;    (11) a method of producing fermentable sugars by obtaining corn kernel from the transgenic plant of (10), and hydrolyzing both the starch and the fiber in the kernel simultaneously after the kernel is ground;    (12) a method of fractionating corn grain into a high starch portion and high fiber-containing bran portion by obtaining the corn kernel of (10) and separating the grain into a high starch portion and a high fiber-containing bran portion;    (13) a method of producing fermentable sugars comprising providing the starch portion of the kernel of (10) and hydrolyzing the starch portion using amylase enzymes;    (14) a method of producing higher levels of fermentable sugars compared to levels producible from non-transgenic corn grain comprising providing the bran portion of corn kernel of (10) and hydrolyzing cellulose and hemicellulose present in the corn fiber with endogenous cellulase or hemicellulase;    (15) a method to reduce the recalcitrance of plant biomass to pretreatment and hydrolysis;    (16) a method for cost-effective processing of lignocellulosic biomass by pretreatment of a plant part under conditions that promote hydrolysis of cellulose and hemicellulose to fermentable sugars, where the plant part is obtained from a transgenic stay-green plant;    (17) a transgenic stay-green plant comprising the expression vector, where the plant produces cellulase enzyme, hemicellulase enzyme, or both in the grain;    (18) a method of producing fermentable sugars by providing corn kernel obtained from the transgenic plant of (17), and hydrolyzing both the starch and the fiber in the kernel simultaneously after the kernel is ground;    (19) a method of fractionating grain into a high starch portion and a high fiber-containing bran portion by obtaining grain from the transgenic plant of (17) and separating the grain into a high starch portion and high bran portion;    (20) a method of producing fermentable sugars comprising providing the starch portion of grain of (17) and hydrolyzing the starch using endoplant amylase enzymes;    (21) a method of producing higher levels of fermentable sugars compared to non-transgenic corn grain by providing the brain portion of corn kernel of (17) and hydrolyzing cellulose and hemicellulose present in the corn fiber with endogenous cellulase or hemicellulase;    (22) a transgenic plant, the genome of which comprises at least one stay green gene and a recombinant polynucleotide encoding at least one lignocellulolytic enzyme polypeptide linked to a promoter sequence, where expression of at least one stay-green gene in the plant is altered; and    (23) a method for separating the kernel into a high starch portion and a high fiber-containing bran portion.