TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation (Disclosed): The expression cassette is prepared by standard recombinant methods. Preferred Sequence: The polycistronic nucleotide sequence further encodes a microbial cell surface protein containing at least one receptor site for binding to a ligand site in the scaffoldingg protein or fusion with scaffoldingg protein. Preferred Protein: The cell surface anchoring protein contains at least two receptor sites each for binding with scaffoldingg protein. The scaffolding protein contains at least nine, preferably four receptor sites for binding with the enzymes. The at least two enzymes are chosen from cellulosomal enzyme, protease, nuclease, lipase, laccase, and amylase, and each enzyme contains a dockerin domain for binding to one of the cohesin domains in the scaffoldingg protein. The cell surface anchoring protein is a cellulosomal surface anchoring protein containing a cohesin domain as the receptor site and the scaffoldingg protein is a cellulosomal scaffolding protein containing (a) a dockerin domain as the ligand site for binding to the cohesin domain in the surface anchoring protein and (b) at least two cohesin domains as the receptor sites for binding to the enzymes. The cellulosomal surface anchoring protein contains at least two cohesin domains for binding to the cellulosomal scaffolding protein. The cellulosomal scaffolding protein contains at least nine, preferably four cohesin domains for binding with the enzymes. The cohesin domain in the cellulosomal surface anchoring protein is a Type-II cohesin domain and the dockerin domain in the cellulosomal scaffolding protein is a Type-II dockerin domain. The cohesin domains in the cellulosomal scaffolding protein are Type-I cohesin domains and each of the enzymes contains a Type-I dockerin domain for binding to one of the Type-I cohesin domains in the scaffolding protein. The cellulosomal scaffolding protein is CipA of Clostridium thermocellum. The cellulosomal surface anchoring protein is OlpB of C.thermocellum. The enzymes are C.thermocellum cellulosomal enzymes. The scaffoldingg protein is C.thermocellum CipA protein and the enzymes are C.thermocellum CelS, CelK, CelR, CelA, XynC, and XynZ proteins. Preferred Promoter: The promoter is a thermo-inducible promoter. Preferred Host: The host cell is mesophilic cell Bacillus subtilis. The expression cassette in the host cell expresses C.thermocellum CipA protein and C.thermocellum cellulosomal CelS, CelK, CelA, XynC, and XynZ proteins. Preferred Biomass: The biomass contains cellulose and the expression cassette in the host cell expresses at least two cellulosomal enzymes. EXAMPLE - No suitable example given.