TECHNOLOGY FOCUS - ENVIRONMENT - Continuously processing dye waste water by using fixed P. chrysosporium and P. putida, comprises (a) preparing bacterial cellulose film fixed P. chrysosporium: (i) preparing Gluconacetobacter xylinus: grafting the G. xylinus stored on the oblique surface and having the strain serial number as CGMCC NO: 1.1812 into the 50 mL seed culture medium, where the usage of each components in the seed culture medium in the usage of the seed culture medium, calculated by the percentage is respectively as follows: 2% cane sugar, 1% fructose, 0.5% peptone, 0.3% yeast extract powder, 0.2% disodium hydrogen phosphate dodecahydrate (Na2HPO4.12H2O), 0.15% potassium dihydrogen phosphate (KH2PO4), 0.025% magnesium sulfate heptahydrate (MgSO4.7H2O), 0.15% citric acid; the pH is 6.0; culturing for 2 days in a swinging bed at 150 rounds per minute (rpm) and at 30 degrees C so as to obtain the activated G. xylinus culture medium; and storing in a refrigerator at 4 degrees C for the spare use; (ii) preparing P. chrysosporium spore liquid: grafting the P. chrysosporium stored on the oblique surface and having the strain serial number as CGMCC NO: 5.776 into the 50 mL seed culture medium, where the usage of each components in the solid culture medium in the usage of the solid culture medium, calculated by the percentage is respectively as follows: 0.38% potato glucose agar, 0.3% KH2PO4, 0.15% MgSO4.7H2O, 0.0005% vitamin B1; culturing for 5 days at 30 degrees C; amplifying the P. chrysosporium spores in a large number; lightly washing the conidiospore on the surface of the flat plate with the germ-free water; filtering the bacteria block by using 16-layer filtering cloth at the germ-free condition so as to obtain the milky spore suspension as the P. chrysosporium spore solution; and storing in a refrigerator at 4 degrees C for the spare use; and (iii) immobilizing P. chrysosporium: respectively grafting the G. xylinus culture solution obtained in step (i) and the P. chrysosporium spore solution obtained in step (ii) into the 150 mL fermentation culture medium, where the volume ratio of the G. xylinus culture solution, the P. chrysosporium spore solution and the fermentation culture medium is 1:0.5:25; the usage of the each components in the fermentation culture medium in the usage of the fermentation culture medium, calculated by the percentage is respectively as follows: 3% cane sugar, 2% fructose, 0.5% peptone, 0.3% yeast extract, 0.2% Na2HPO4.12H2O, 0.15% KH2PO4, 0.025% MgSO4.7H2O, 0.15% citric acid; the pH is 6.0; placing and culturing for 7 days at 30 degrees C so that the newly generated bacterial membrane cellulose film is absorbed with plenty of P. chrysosporium; washing the film for many times by using the normal saline so as to obtain the bacterial membrane cellulose film fixed P. chrysosporium; and storing in a refrigerator at 4 degrees C for the spare use, where the G. xylinus, the P. chrysosporium and the P. putida come from the China Preservation and Management Center of Normal Microorganism Strains; (b) preparing bacterial membrane cellulose film fixed P. putida: (i) preparing bacterial membrane cellulose film block: grafting 7 mL G. xylinus culture solution obtained in the step (a)(i) into the 125 mL fermentation culture medium; placing and culturing for 7 days at 30 degrees C; generating a faint yellow gel film on the liquid surface; taking out the film; washing repeatedly by using the distilled water; removing the culture medium on the surface of the film; immerging the film into the 0.1 mol/L sodium hydrogen (NaOH) solution; boiling for 20 minutes at 100 degrees C; removing the thallus and the residual culture medium inside the bacterial film; repeatedly washing the film with the distilled water until it is neutral; cutting the obtained bacterial membrane cellulose film into film blocks with the grain size of 1 cm3; and storing the blocks into the refrigerator at 4 degrees C for the spare use; and (ii) immobilizing P. putida: grafting the P. putida stored on the oblique surface and having the strain serial number as CGMCC NO: 1.1003 to the 100 mL P. putida culture medium including bacterial membrane cellulose film into film blocks with the grain size of 1 cm3, where the volume ratio of the bacterial membrane cellulose film into film block to the P. putida culture medium is 1:5; the usage of each component in the P. putida culture medium in the usage of the P. putida culture medium culture medium, calculated by the percentage is respectively as follows: 0.35% ammonium sulfate ((NH4)2SO4), 0.75% potassium dihydrogen phosphate trihydrate (KH2PO4), 2.216% dipotassium hydrogen phosphate trihydrate (K2HPO4.3H2O), 0.025% MgSO4.7H2O, 1.81% sodium citrate; and the pH is 7.0; culturing for 3 days at the water bath swinging bed at 150 rpm and at 30 degrees C; attaching P. putida to the bacterial membrane cellulose film block; washing for many times with the germ-free normal saline so as to obtain the bacterial membrane cellulose film fixed P. putida; and storing in a refrigerator at 4 degrees C for the spare use, where the P. putida during the operation process comes from the China Preservation and Management Center of Normal Microorganism Strains and has the serial number as 1.1003; (c) preparing dye waste water: weighing 30 mg methyl orange, 20 mg congo red, 20 mg methylene blue, and 2 mg malachite green; and mixing them; respectively dissolving the mixture into 1 L coupled culture medium, where the components in the coupled culture medium in the total usage of the coupled culture medium, calculated by the percentage are respectively as follows: 0.75% KH2PO4, 2.216% K2HPO4.3H2O, 0.25% (NH4)2SO4, 0.025% MgSO4.7H2O; 0.005% manganese sulfate (MnSO4), 0.1% anhydrous ferrous sulfate (FeSO4), 0.001% sodium chloride (NaCl), 0.1% calcium chloride (CaCl2), 1.81% sodium citrate, 5% glucose, 0.0005% vitamin B1; and the pH is 7.1; obtaining the dye waste water; scanning by the spectrum so as to obtain the maximum absorption wavelength as 505 nm; and (d) continuously treating dye waste water by using fixed P. chrysosporium and P. putida: controlling the water bath temperature of the processing device at 28-32 degrees C; putting the prepared bacterial membrane cellulose film fixed P. chrysosporium and the P. putida into the corresponding fixed P. chrysosporium reactors I and II and the fixed P. putida reactor; enabling the volume ratio of the dye waste water in each reactor to the fixed bacteria to be 1:(0.08-0.12); starting up a peristaltic pump so as to enable the waste water to come into the treatment device; controlling the flow speed of the incoming water flow of the waste water as 3-7 mL/minute; after the waste water flows out of the water outlet, starting up the peristaltic pump; controlling the dissolution oxygen amount at 4-8 mg/L; and testing the chroma and the chemical oxygen demand (CODcr) removal rate in the dye waste water every 24 hours.