NOVELTY - Method for constructing full-cell lipase preparation, involves designing Flo gene clone primer, amplifying the genome DNA of Saccharomyces cerevisiae as template PCR into Flo gene section using SfiI and EcoRI restriction enzyme, designing the primers, amplifying the primers, digesting EcoRI using Flo gene section, ligating Flo gene section with LIPY7 gene section, amplifying the primers, digesting the smelting gene, expressing the obtained vector in Candida lipolytica and transforming C.lipolytica to obtain product.    USE - The method is useful for constructing full-cell lipase preparation used for degrading oil in oily sewage (claimed) and in soil polluted by oil.    ADVANTAGE - The lipase preparation is environmentally-friendly.    DETAILED DESCRIPTION - Method for constructing full-cell lipase preparation, involves (a) designing Flo gene clone primer according to the gene coding sequence (NC001133) of Flo film proteins Flo-F-SfiI and Flo-R-EcoR, amplifying the genome DNA of Saccharomyces cerevisiae as template PCR into Flo gene section using SfiI and EcoRI restriction enzyme, (b) designing the primers according to the sequence of lipase gene LIPY7, LIPY7f-Mun I and LIPY7r-XbaI, amplifying the primers through PCR to obtain LIPY7 gene section with MunI and XbaI restriction enzymes, digesting EcoRI using Flo gene section and digesting LIPY7 gene section using MunI to obtain the gene section at the tail end with the same stickiness, where EcoRI and MunI are isocaudarner, (c) ligating the Flo gene section digested by EcoRI with LIPY7 gene section digested by MunI using T4DNA ligase, amplifying the primers Flo-F-SfiI as positive primer and Flo-R-EcoR as negative primer using the ligated product as template to obtain Flo-LIPY7 smelting gene section having SfiI and XbaI restriction enzyme at two ends, (d) digesting the smelting gene using SfiI and XbaI, ligating the digested product in expression vector pINA1296 digested by SfiI and XbaI to obtain the vector pINA1296-F-LIPY7, expressing the obtained vector in Candida lipolytica, (e) linearly transforming recombinant plasmid pINA1296-F-LIPY7, transforming C.lipolytica and detecting through indirect immunofluorescence to determine expression of lipase LIPY7 on the surface of C.lipolytica cell to obtain full-cell lipase preparation of lipase LIPY7.