NOVELTY - A Bacillus subtilis ANSB01G (CGMCC 4297), is new.    USE - The B.subtilis is useful for preparing active protein for degrading zearalenone, and/or cellulose, and suppressing Salmonella pullorum, Escherichia coli and/or Staphylococcus aureus (all claimed) in feed.    ADVANTAGE - The B.subtilis has high activity, strong specificity and mild action effect, does not damage nutrient components in feed, improves transferring rate of feed, exhibits degradation rate of 83%, and is capable of resisting gastric acid and/or cholate.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) method (M1) for culturing the B.subtilis, involves obtaining 0.5-2.5 ml of B.subtilis with viable bacteria concentration of 109 colony forming units (CFU)/ml, inoculating the B.subtilis into a culturing medium (50-100 ml), shaking the culture medium, fermenting and culturing the B.subtilis, where the fermentation culturing medium comprises (in g): tryptone (8-12), yeast extract (1.5-3), glucose (1.5-4), beef extract (2-5), sodium chloride (3-6), disodium hydrogen phosphate (2.5-4) and magnesium sulfate heptahydrate (0.5-1.5), and adding distilled water to culture liquid (800-1200 ml), where the pH value of the culture liquid is 7-7.4; (2) method (M2) for degrading zearalenone, involves obtaining fermented liquid (600-800 mu l) of B.subtilis, adding 500 parts-per-billion (ppb)-zearalenone (150-200 mu l), adjusting the pH value of the reaction system to 7-7.4, and reacting the mixture for 2-72 hours at 36-38 degrees C; (3) method (M3) for preparing active protein for degrading zearalenone, involves obtaining fermented liquid of the B.subtilis, centrifuging and obtaining liquid supernatant, adding 2-4 times of absolute ethyl alcohol to supernatant for 0.5-2 hours, centrifuging the obtained mixture, washing the obtained product using phosphate buffered saline (PBS) (0.01 mol/l) of buffer liquor, centrifuging obtained product, dissolving the obtained product in PBS (0.01 mol/l) of buffer liquid, and freeze-drying and concentrating the residue to obtain active protein; and (4) method (M4) for degrading cellulose, involves obtaining the B.subtilis, and inoculating the B.subtilis in culturing medium (25-100 ml) containing cellulose as carbon source, fermenting the culture at 36-38 degrees C, pH value of 7.0-7.4 and 180-220 revolutions/minute (rpm) for 20-30 hours.