NOVELTY - Method for producing biofuel, involves treating raw materials through microorganism cell culture solution, fermenting the solution, distilling the fermented solution, and dehydrating the distillate.    USE - The method is useful for producing biofuel, preferably ethanol.    DETAILED DESCRIPTION - Method for producing biofuel, involves treating raw materials using microorganism cell culture solution, fermenting the solution, distilling the fermented solution, and dehydrating the distillate. The microorganism cell culture solution is fungoid, bacteria, saccharomycetes or actinomycetes. The raw materials and auxiliary materials for producing multienzyme system cell culture solution comprises (in parts weight): carbon source (3-40), nitrogen source (1-10), nutritional salt (1-5), auxin (1-5) and water (40-94). The multienzyme system cell culture solution is prepared by (a) adding the raw materials and auxiliary materials into a special culture container, and maintaining the feeding constant volume of the container to 30-90%, (b) passing air into the container by air compressor and purifying system, and setting the air volume of the air to 0.1-1.4 l/l minute, (c) inoculating the culture solution with microorganism strain into special culture container, and maintaining the inoculum size to 5-30%, (d) culturing the microorganism at 20-39 degrees C, and at pH of 3-8.5 for 40-150 hours, and stopping the culturing step when dextrose equivalent (DE) value of the culture reaches 0.1-20%, and (e) adding the culture solution and the raw materials in the volume ratio of 0.0001-0.1:1, and processing the mixture at 30-105 degrees C and at pH of 3-10. The ethanol is prepared by (a) transferring yeast into a fermenting tank, (b) transferring the treated material into a culture medium, (c) culturing the solution in one or multiple container connected in series or in parallel, and (d) adding the solution into a biofuel production system, liquefying and saccharifying the solution, and hydrolyzing the saccharified solution. The enzyme is chosen from amylase, alpha -amylase, beta -amylase, fungal amylase, isoamylase, saccharifying enzyme, transglucosidase, cellulase, hemicellulase, lignin enzyme, xylanase, glucanase, pectinase, protease, lipase and lysozyme.