TECHNOLOGY FOCUS - BIOLOGY - Preferred Components: The ascidian is Ciona intestinalis.    TECHNOLOGY FOCUS - INSTRUMENTATION AND TESTING - Preferred Method: In the method (M') for farming ascidians, several elongate members comprise at least one elongate support member and several colonization members, where each colonization member is coupled to the support member and comprises at least one smooth and uninterrupted colonization surface. The colonization surface is planar, and is horizontal. The colonization members are cylinders. The several elongate members comprise at least one elongate member, which supports several generally radially extending colonization surfaces or members. The generally radially extending colonization surfaces or members are disc or rectangular-shaped, or are cylinder-shaped. The colonization surfaces or members are dark in color. At least one colonization surface is perforated or formed of a mesh. Preferred Apparatus: In the tunicate colonization apparatus, at least one of the colonization surfaces is planar, and at least one colonization surface is perforated or formed of a mesh. The colonization members are in the form of cylinders.    TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Method: In the method for production of at least one biofuel, the cellulose obtained from a tunicate is used for the production of ethanol, and the lipids/fatty acids obtained from a tunicate is used for the production of biodiesel. The method for production of biofuel selected from alcohol and biodiesel from tunicate, involves: (a) (i) subjecting the tunicate or at least one polysaccharide extracted from the tunicate to enzymatic or acid hydrolysis to form a hydrolysate containing at least one monosaccharide, and (ii) fermenting at least one monosaccharide to form an alcohol; or (b) (i) extracting lipids/fatty acids from the tunicate, and (ii) converting the lipids/fatty acids into biodiesel by transesterification or alcoholysis, or (iii) subjecting the tunicate to transesterification or alcoholysis to convert lipids/fatty acids present in the tunicate into biodiesel. The step (i) of step (a) involves extracting at least one polysaccharide from the tunicate before subjecting at least one polysaccharide extracted from the tunicate to enzymatic or acid hydrolysis to form hydrolysate containing at least one monosaccharide, and the method for producing alcohol from tunicate further involves producing biodiesel from the tunicate by the step (i) of step (b), and prior to the step (i) of step (a), step (i) of step (b), or step (iii) of step (b), the tunicate is dried; or the step (i) of step (a) involves subjecting the tunicate to enzymatic or acid hydrolysis and further involves a step of drying the tunicate to above 50% dryness content prior to subjecting it to enzymatic or acid hydrolysis). In the step (i) of step (b), prior to transesterification, the lipids/fatty acids extracted from the tunicate are subjected to an esterifcation process so that the fatty acids are reacted with an alcohol in the presence of an acid catalyst to form fatty acid alkyl esters. In the step (iii) of step (b), prior to transesterification, the tunicate is subjected to an esterifcation process so that the fatty acids are reacted with an alcohol in the presence of an acid catalyst to form fatty acid alkyl esters. In the method for producing an alcohol from a tunicate by the step (i) of step (a) and additionally producing biodiesel from the tunicate, the method involves extracting lipids/fatty acids from the hydrolysate formed in step (i) of step (a) or a fermentation broth formed in step (i) of step (a) and converting the lipids/fatty acids into biodiesel by transesterification or alcoholysis. The method for producing biodiesel from a tunicate by step (ii) of step (b) or step (iii) of step (b), additionally involves producing an alcohol from the tunicate, where the method involves obtaining a tunicate residue from the extraction of lipids/fatty acids from the tunicate in step (i) of step (b) or from a recovery of biodiesel from the product of step (ii) of step (b) and subjecting the tunicate residue or at least one polysaccharide extracted from the tunicate residue to enzymatic or acid hydrolysis to form a hydrolysate containing at least one monosaccharide and fermenting at least one monosaccharide to form an alcohol. The tunicate is an ascidian. The method for producing biofuel from the ascidian involves the method of farming the ascidian by the method (M'). EXAMPLE - Biodiesel production was carried out as follows: extraction of lipids from tunicate and transesterification of extracted lipids was carried out as follows. For extraction of lipids from dried tunicate a sample of tunicate was freeze dried. The dried tunicate (106 g) was subjected to extraction in Soxhlet equipment using petroleum ether (30-60 degrees C) (800 ml) for 6 hours. The resultant product was filtered to separate the solution (filtrate) from the solid tunicate material. The filtrate was evaporated under vacuum using a rotary evaporator. Lipids (3.2 g) were obtained (3 wt.% yield based on weight of dried tunicate). For base catalyzed transesterification of extracted lipids, KOH (50 mg) was added into methanol (20 ml) under vigorous stirring. The solution obtained was then added to the lipids (1 g) obtained in the previous step. The mixture was heated to reflux and kept under reflux for 4 hours. The mixture was then cooled and left to stand. After which, upper layer was separated out and evaporated under vacuum using a rotary evaporator. Water (10 ml) was then added followed by adding CH2Cl2 (10 ml). After vigorously shaking and then standing, the bottom layer was separated out and evaporated and biodiesel (0.7 g) was obtained. When multiplied up this equated to a yield of biodiesel (2.1 wt.%) based on the weight of the dried tunicate.