NOVELTY - A method of fractionating biomass involves the steps of permeability conditioning biomass suspended in a pH adjusted solution of at least one water-based polar solvent to form a conditioned biomass; intimately contacting the conditioned biomass with at least one non-polar solvent; partitioning to obtain a non-polar solvent solution and a polar biomass solution; recovering cell products from the non-polar solvent solution and polar biomass solution; and fractionating polar and water soluble components from the biomass.    USE - As method of fractionating biomass, where the biomass is selected from fungi, bacteria, yeast, mold, microalgae, macroalgae and related high moisture agricultural products; for obtaining end product selected from biocrude, soluble monosaccharides, disaccharides, oligosaccharides, polysaccharides, glycerols, amino acids, soluble proteins, peptides, fiber, nutrients, phosphocholine, phosphate, growth media, and residual solid algae cell structural particles, where biocrude includes terpenoids such as sterols and carotenoids, chlorophyll, phospholipids, glycolipids, sphingolipids, triacylglycerols, diacylglycerols, monoacylglycerols, fatty acids, decarboxylated fatty acid hydrocarbon chains; methyl, ethyl, propyl, butyl and/or amyl esters of the fatty acids, aromatics, alkyl aromatics, polyaromatics, naphthalene, alkyl substituted naphthalene, linear and branched alkanes, linear and branched alkenes, alcohols such as methanol, ethanol, butanol, and other lipid compounds (claimed); and for deriving biofuels and other valuable end products. The biomass also includes agricultural products such as corn stalks, straw, seed hulls, sugarcane leavings, bagasse, nutshells, and manure from cattle, poultry, and hogs, wood materials such as wood or bark, sawdust, timber slash, and mill scrap, municipal waste such as waste paper and yard clippings, or crops, such as poplars, willows, switchgrass, alfalfa, prairie bluestem, corn, and soybean.    ADVANTAGE - The method efficiently derives end products from biomass such as algae biomass. The method provides efficient and inexpensive method of raising algae; provides efficient and flexible fractionation platform that can achieve success at isolating classes and specific products and in addition recover on a large scale all valuable products efficiently from the algal biomass. The method not only allows extraction of target classes and products but also recovery of by-products and recycling of critical nutrients and water. The method provides flexible and efficient fractionation platform that allows not only targeted product isolation and preconditioning of the products, but also effective separation of by-products. The method allows fractionation of major components of biomass containing membrane-bound lipids on large scale. Unlike prior art processes that use high energy disruption followed by general lipid extraction to extract a single product, the fractionation process includes conditioning process, contacting process and partitioning process that allows formation of end products.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following:    (1) a method (M1) of fractionating biomass involving permeability conditioning biomass suspended in a pH adjusted solution of at least one water-based polar solvent with at least one enzyme to form a conditioned biomass; intimately contacting the conditioned biomass with at least one non-polar solvent; partitioning to obtain a non-polar solvent solution and a polar biomass solution; and obtaining an end product selected from biocrude, soluble monosaccharides, disaccharides, oligosaccharides, polysaccharides, glycerols, amino acids, soluble proteins, peptides, fiber, nutrients, phosphocholine, phosphate, growth media, and residual solid algae cell structural particles; and    (2) biocrude obtained from the method (M1), where the biocrude includes terpenoids such as sterols and carotenoids, chlorophyll, phospholipids, glycolipids, sphingolipids, triacylglycerols, diacylglycerols, monoacylglycerols, fatty acids, decarboxylated fatty acid hydrocarbon chains; methyl, ethyl, propyl, butyl and/or amyl esters of the fatty acids, aromatics, alkyl aromatics, polyaromatics, naphthalene, alkyl substituted naphthalene, linear and branched alkanes, linear and branched alkenes, alcohols such as methanol, ethanol, butanol, and other lipid compounds.