NOVELTY - Hyperin is separated from hawthorn leaves or stems by performing ethanol extraction on crushed hawthorn leaves or stems, filtering and concentrating the extraction solution; adsorbing on AB-8 or D101 macroporous adsorption resin, eluting with ethanol; concentrating the eluate, drying; subjecting into polyamide column chromatography, performing gradient elution with ethanol, concentrating the eluate, drying; and subjecting into silica gel column chromatography separation and ethyl acetate-butanone-formic acid-water or ethyl acetate-acetone-formic acid-water elution to obtain hyperin powder.    USE - Method for separating hyperin from hawthorn leaves or stems (claimed).    ADVANTAGE - The method is simple, practical, environment-friendly, and capable of providing hyperin product with high purity (90-99 %) and high yield, thus making it suitable for industrial production.    DETAILED DESCRIPTION - Hyperin is separated from hawthorn leaves or stems by (1) adding crushed hawthorn leaves or stems with 16-24 times of aqueous ethanol solution having 30-80 mass % concentration to extract 2-3 times for 1-3 hours each time; filtering the extraction solution, combining the filtrates; decompressing and recycling out the ethanol to obtain concentrated solution; (2) adsorbing the concentrated solution on AB-8 or D101 macroporous adsorption resin to remove impurity; eluting with ethanol having 30-80 mass % concentration until flavone is no longer detected; collecting the eluate, concentrating and recycling out the ethanol, drying; (3) subjecting the dried matter into polyamide column chromatography, performing gradient elution with ethanol having 30-80 mass % concentration at a dosage of 3-4 times the volume of the column, decompressing and concentrating the eluate, drying; (4) subjecting the dried matter into 200-300 mesh silica gel column chromatography separation and ethyl acetate-butanone-formic acid-water or ethyl acetate-acetone-formic acid-water elution to directly obtain hyperin powder.