NOVELTY - Method for biologically detoxifying pre-treated lignocellulosic biomass, involves inoculating Amorphotheca resinae strain ZN1 into potato dextrose agar (PDA) substrate, culturing the strain, inoculating the strain into pre-treated lignocellulosic raw material, culturing the strain until the hypha is formed so as to be used as first stage seed, inoculating cellulose and alcoholic fermentation strain into biologically detoxified lignocellulose and performing synchronous saccharification and fermentation to produce fuel ethanol.    USE - The method is useful for biologically detoxifying pre-treated lignocellulosic biomass.    ADVANTAGE - The method is simple to operate and cost-effective, and can be performed within short period of time.    DETAILED DESCRIPTION - Method for biologically detoxifying pre-treated lignocellulosic biomass, involves (a) washing and drying lignocellulosic raw material, pulverizing the raw material using crusher to 0.1-20 mm, treating the powder in a vapor blasting device for 1-15 minutes, where the temperature is 160-240 degrees C and pressure is 0.3-2.0 MPa, or reacting the lignocellulosic raw material in a dilute acid pre-treatment reactor for 1-15 minutes after pre-soaked in dilute sulfuric acid (0.5-5 weight/weight%), where the temperature is 160-240 degrees C, and (b) inoculating Amorphotheca resinae strain ZN1 into potato dextrose agar (PDA) substrate, culturing the strain at 20-30 degrees C, eluting spore of the strain, diluting and inoculating the strain into pre-treated lignocellulosic raw material, culturing the strain until the hypha is formed so as to be used as first stage seed, inoculating the cultured first stage seed into the pre-treated lignocellulosic raw material, where pH of the raw material is adjusted to 4-7 and water content to 10-90%, culturing the strain for 1-7 days at 20-40 degrees C, inoculating cellulose and alcoholic fermentation strain into biologically detoxified lignocellulose and performing synchronous saccharification and fermentation to produce fuel ethanol.