TECHNOLOGY FOCUS - BIOLOGY - Preferred Components: The fermentation raw material comprises food waste containing starch raw material, restaurant-kitchen garbage, and starch pulp in starch processing industry and waste molasses in sugar industry. The weight ratio of solid to liquid of fermentation raw material is 1:1-1:5, and pH is 4.0-5.5. Preferred Method: The method for pre-treating the food waste, involves collecting and sorting food waste to remove large sundries, pulverizing the food waste to particle diameter of 1-10 mm through food crusher, and uniformly mixing the food waste. The method involves adding saccharifying enzyme (100-300 U/g) during the saccharifying process, and performing saccharification process at 50-65 degrees C for 30-60 minutes. The fermentation culture medium is obtained by mixing food waste hydrolysate with water, adjusting concentration of reducing sugar to 60-100 g/l and pH to 6-7, sterilizing the obtained mixture at 115-121 degrees C for 15-20 minutes and cooling the obtained product. The zymogenous bacteria is obtained by activating freeze-dried strain by adding water in corn flour (5-8 g) and mixing the obtained mixture, slowly pouring the solution to boiled water (100 ml) and continuously mixing, cooking for 1 hour and packaging the obtained product in test tube, sterilizing the material at 121 degrees C for 1 hour, cooling the sterilized material to 30-40 degrees C, inoculating the strain in frozen tube, placing the inoculating test tube in boiled water bath for 1-2 minutes, placing the obtained test tube in cold water for 1-2 minutes, placing the test tube in vacuum dryer, and placing the test tube in anaerobic culture medium to culture at 37 degrees C for 18-24 hours or at vacuum degree of 1 MPa. The method involves inoculating activated strain suspension (3.5 ml) in 100 ml blood serum anaerobic bottle filled with tryptone-yeast extract-agar (TYA) culture liquid (70 mol) under sterile condition, culturing in anaerobic culture box at 37 degrees C for 18-20 hours to prepare zymogenous bacteria. The method involves inoculating the zymogenous bacteria (3-10%) in fermentation broth culture medium, where the material filling dosage of fermentation material is 2/5-4/5, culturing the fermentation material in anaerobic culture box at 30-37 degrees C for 70-120 hours to obtain biofuel.    TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Microorganism: The zymogenous bacteria is chosen from C.acetobutylicum strain ATCC824, C.saccharoperbutylacetonicum strain ATCC13564 and C.beijerinckii strain BA101.