TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Algae: The algae have a maximum growth rate at least 25% greater than algae of the same species that has not been EMO to utilize raw glycerol as a primary carbon source, and where the algae was able to grow on glycerol in a refined glycerol test prior to being EMO. It is composed of 15-90% of lipids and/or hydrocarbon. The primary carbon source consists of raw glycerol. The algae have a maximum growth rate at least 25% or 50% greater than algae of the same species that has not been EMO to utilize raw glycerol as a primary carbon source. The algae are not modified by recombinant DNA techniques. Preferred Method: Processing raw glycerol further comprises recovering the grown algae; fractionating the grown algae to obtain a first lipid fraction; and transesterifying the first lipid fraction with an alcohol to obtain an alkyl ester fraction and a second raw glycerol fraction; and recovering the alkyl esters. It also comprises recovering a protein fraction from the fractionating step; adding the second raw glycerol fraction to the first raw glycerol fraction so that the first and second raw glycerol fractions are processed in the processing step; and filtering the alkyl esters to obtain a filtered biodiesel product. The first raw glycerol fraction is obtained from a transesterification reaction. In producing biodiesel product from by-product of biodiesel production, the first by-product of biodiesel production is obtained from a transesterification reaction. The method further comprises adding the second by-product of biodiesel production to the first by-product of biodiesel production in the growing step; filtering the alkyl esters to obtain a biodiesel product; and recovering a protein fraction or other chemical compound from the fractionating step. Preferred Site: The biodiesel manufacturing site further comprises a protein recovery unit for recovering protein from the algae after the algae has been processed in the processing unit. The culture unit has a growth chamber for growing the algae in a heterotrophic environment. The biodiesel manufacturing site also comprises a filter unit for recovering and filtering the alkyl esters to obtain a biodiesel product. EXAMPLE - No suitable example given.