TECHNOLOGY FOCUS - BIOLOGY - Preferred Method: The disrupting stage further includes pressure cycling by injecting the oxidizer agent into the biomass sufficient to pressurize the biomass to a first elevated pressure and then depressurizing the biomass. The pressure cycling is repeated 1 to 4 times. Each of the first elevated pressure is 0-30 psi and second elevated pressures are at least 30 psi. Each of the first elevated pressure and the second elevated pressure is 80-200 psi. The first elevated pressure is different from the second elevated pressure. The injecting and depressurizing steps of the disrupting stage are performed 1 to 15 times (preferably 6 to 10 times). The injecting and depressurizing of the rupture stage produces a pressure differential of at least 50 psi. The depressurizing leaves the biomass at ambient pressure. The contaminated sample is agitated by at least one of stirring, shaking, and ultrasound during at least one of steps injecting, and depressurizing. The method occurs in a reactor vessel having pressurizing and depressurizing time of 20 seconds to 20 minutes for each complete cycle. The process takes place in a reactor that has an occupied volume to headspace volume ratio of 1:9-9:1. The method further involves the step digesting the residual cell contents to form a biofuel. The steps of injecting and depressurizing have a collective duration of 20 seconds to 2 minutes (preferably 25 seconds to 90 seconds). The method further involves a step of equilibrating the sample after the pressurizing step for a period of 10-90 seconds. Preferred Components: The oxidizing agent is an oxidizer gas selected from ozone and/or chlorine. The oxidizer gas includes ozone. The oxidizing agent is a liquid or a solid. The oxidizing agent is potassium permanganate (KMnO4), sodium hypochlorite (NaClO), potassium Ferrate (IV) (K2FeO4), and/or liquid chlorine. The rupture gas is selected from air, carbon dioxide, nitrogen, argon, helium, and/or oxygen. EXAMPLE - No suitable example is given.