TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preparation (claimed): Preparation of alpha-amylase (S1) involves: (a) growing the host cell (C1) under conditions leading to expression of the hybrid alpha-amylase; and (b) recovering the hybrid alpha-amylase. Preferred Compounds: The isolated alpha-amylase (S1) comprises: the A-domain with at least 60% sequence identity with the A-domain and a C-domain with at least 60% sequence identity with the C-domain of any of any of 14 amino acid sequences as given in the specification; e.g. amino acid sequence having 484 amino acids as given in the specification (SEQ ID NO:1); amino acid sequence having 516 amino acids as given in the specification (SEQ ID NO:4); amino acid sequence having 486 amino acids as given in the specification (SEQ ID NO:7); amino acid sequence having 486 amino acids as given in the specification (SEQ ID NO:12); amino acid sequence having 487 amino acids as given in the specification (SEQ ID NO:29); and amino acid sequence having 485 amino acids as given in the specification (SEQ ID NO:30); and a B-domain with at least 60% sequence identity with the B-domain of any of 5 amino acid sequences as given in the specification; e.g. amino acid sequence having 485 amino acids as given in the specification (SEQ ID NO:13); amino acid sequence having 481 amino acids as given in the specification (SEQ ID NO:14); amino acid sequence having 436 amino acids as given in the specification (SEQ ID NO:16); and amino acid sequence having 436 amino acids as given in the specification (SEQ ID NO:31). The A-domain has at least 60 (preferably at least 80, especially at least 90, particularly at least at least 99 or 100)% sequence identity with the A-domain of (SEQ ID NO:4). The A1-domain has at least 60 (preferably at least 80, especially at least 95, particularly at least 99, or 100)% sequence identity with the sequence starting at a position 1-5 and ending a position 94-114, e.g. (starting at a position 1-3 and ending at a position 99-104, or starting at a position 1-3 and ending at a position 104-109, in particular positions 1-104 of (SEQ ID NO:4)). The B-domain has at least 60 (preferably at least 80, especially at least 95, particularly at least 99 or 100)% sequence identity with the sequence starting at a position 93-113 and ending at a position 195-215, e.g. (starting at a position selected from 97-109 and ending at a position selected from 199-211, or starting at a position selected from 100-106 and ending at a position selected from 202-208, in particular positions 103-208 of (SEQ ID NO:13)). The C-domain has at least 60 (preferably at least 80, especially at least 95, particularly at least 99 or 100)% sequence identity with the C-domain of (SEQ ID NO:4). The A2 and C-domains have at least 60 (preferably at least 80, especially at least 95, particularly at least 99 or 100)% sequence identity with the sequence starting at position selected from 201-221 and ending at position selected from 478-483, e.g. (starting at position selected from 206-211 and ending at position selected from 480-483 or starting at position selected from 211-216 and ending at position selected from 480-483, in particular positions 211-483 of (SEQ ID NO:4)). The alpha-amylase (S1) is more thermos table and has reduced calcium sensitivity than the alpha-amylase of any of 14 amino acid sequences as given in the specification, e.g. (SEQ ID NOs:1, 4, 7, 12, 29 and 30). Preferred Process: Producing (M1) the fermentation product involves contacting a starch substrate with the alpha-amylase (S1), a glucoamylase, and a fermenting organism. EXAMPLE - No suitable example is given.