NOVELTY - Preparation of a variant of parent polypeptide, involves (a) providing an amino acid sequence of the parent polypeptide, (b) substituting at least one amino acid residue at position in a sequence corresponding to any positions (P1) chosen from 41, 83, 129, 207 and 284 in an amino acid sequence of SEQ ID NO: 2, (c) selecting a variant with lipolytic activity, and (d) recovering the variant. Sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication.    USE - The method is useful for preparing variant of parent polypeptide for producing fatty acid ethyl esters and fatty acid methyl ester which are used as biodiesel.    ADVANTAGE - The variant has improved stability in presence of elevated temperatures, oxidizing conditions, alcohol, or their combinations, improved specific activity and improved transesterification activity.    DETAILED DESCRIPTION - Preparation of a variant of parent polypeptide, involves (a) providing an amino acid sequence of the parent polypeptide, (b) substituting at least one amino acid residue at position in a sequence corresponding to any positions (P1) chosen from 41, 83, 129, 207 and 284 in an amino acid sequence of SEQ ID NO: 2, (c) selecting a variant with lipolytic activity, where the variant has improved property compared to the parent polypeptide, and comprises an amino acid sequence with at least 60% identity to the mature polypeptide of SEQ ID NO: 2, and (d) recovering the variant. Sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication. INDEPENDENT CLAIMS are included for the following:    (1) isolated variant of the parent polypeptide, chosen from (1) polypeptide comprising a substitution at positions (P1) with at least one amino acid residue, where the variant comprises an amino acid sequence with at least 65%, preferably 99% identity to the mature polypeptide of SEQ ID NO: 2, (2) polypeptide encoded by a polynucleotide that is capable of hybridizing under at least low stringency conditions with (i) a mature polypeptide encoding base pair sequence of SEQ ID NO: 1, (ii) genomic DNA sequence comprising coding sequence of mature polypeptide (P2), or (iii) full-length complementary strand of (i) or (ii), and (c) polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60%, preferably 99% identity with the mature polypeptide (P2);    (2) isolated polynucleotide encoding the above-mentioned variant;    (3) nucleic acid construct comprising the above-mentioned isolated polynucleotide;    (4) expression vector or host cell comprising the above-mentioned nucleic acid construct; and    (5) composition comprising the above-mentioned variant.