TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: The method (M1) involves producing biological oil in which greater than 50% of unsaturated fatty acids are polyunsaturated fatty acids. The microorganism is Thraustochytrid. The microorganism is chosen from microorganisms of genus Schizochytrium, Thraustochytrium, and Ulkenia. The microorganism saccharifies cellulose. The microorganism is resistant to or degrades feedstock components chosen from lignin, hemicellulose, plant oil, plant extracellular polysaccharides, and their combinations. The microorganism is genetically modified microorganism. The microorganism produces 25-85 wt.% of oil in the form of triglyceride. The method (M1) further involves performing autolysis or induced lysis of the microorganism after producing oil of 30-90 wt.%. The method (M1) further involves inducing lysis of the microorganism by exposing the microorganism to a condition favorable for lysis chosen from pH, temperature, presence of enzyme, presence of detergent, physical disruption, and their combinations. The feedstock comprises source of cellulose chosen from grass, sugar cane, agricultural waste, waste paper, sewage, wood, organism of kingdom viridiplantae, and their combinations. The fermentation process is performed in nonsterile fermentor. The method (M1) involves carrying out fermentation in a fermentor chosen from fiber reinforced polymer fermentor, metal matrix composite fermentor, ceramic matrix composite fermentor, thermoplastic composite fermentor, metal fermentor, epoxy lined carbon steel fermentor, plastic lined carbon steel fermentor, plastic fermentor, fiberglass fermenter, and concrete fermentors. The fermentation process is carried out in a fermentor that is submerged in water. The fermentation is carried out in fermentors having cooling systems connected in series such that cooling water effluent from first fermentor or set of fermentors in the series is used as a cooling water supply for second fermentor or set of fermentors in the series. The fermentation is carried out in fermentors having gas systems connected in series such that sparge exhaust from first fermentor or set of fermentors in the series is used as a gas supply for second fermentor or set of fermentors in the series. The transesterifying process of biological oil is performed using alcohol derived from alcohol production process. The method (M2) further involves using glycerol resulting from transesterifying biological oil as carbon source for subsequent fermentation process to produce alcohol or biological oil, where the subsequent fermentation process grows microorganism that is capable of using glycerol as carbon source. The biological oil comprises 10-75 wt.% of polyunsaturated fatty acids. Preferred Composition: The lipid-based biofuel composition has a melting temperature 30 degrees C to -50 degrees C. The biological oil is produced at a rate of 5-70, preferably 30-70 g/L/day. The method (M1) involves growing microorganism, where the microorganism achieves a cell density of 10-300, preferably 150-250 g/L. The recycled media is chosen from de-lipidated biomass, hydrolyzed biomass, partially hydrolyzed biomass, recycled metals, recycled salts, recycled amino acids, recycled extracellular carbohydrates, recycled glycerol, recycled yeast biomass, and their combinations. The continuous seed stage produces 10-95% of total biomass of microorganism. The lipid-production stage is carried out as fed-batch process. The lipid-production stage produces lipids 10-95% of total lipid of said microorganism. EXAMPLE - No suitable example given.