TECHNOLOGY FOCUS - BIOLOGY - Preferred Components: The lipase is yeast lipase, Rhizopus lipase, Pseudomonas lipase or porcine pancreatic lipase.    TECHNOLOGY FOCUS - INORGANIC CHEMISTRY - Preferred Method: The continuous production of bio-diesel using lipase comprises: (i) performing first catalytic transesterification by adding vegetable fats and oils and 1/3rd of lower alcohol into a first mixing tank and mixing thoroughly, transferring the pretreated fats and oils mixture into the fixed bed reactor at 40 plus minus 3 degrees C, passing the reaction products into the storage tank and into the liquid-liquid swirler through a high-pressure pump to separate glycerol; (ii) performing second catalytic transesterification by adding the reaction product and 1/3rd of lower alcohol into a second mixing tank for mixing thoroughly, transferring the mixtures into the fixed bed reactor at 40 plus minus 3 degrees C, passing the reaction products into the storage tank and into the liquid-liquid swirler through a high-pressure pump to separate glycerol; (iii) performing third catalytic transesterification by adding reaction product and 1/3rd of lower alcohol into a third mixing tank for mixing thoroughly, transferring the mixtures into the fixed bed reactor at 40 plus minus 3 degrees C, passing the product into the storage tank and into the liquid-liquid swirler through a high-pressure pump to separate glycerol; and (iv) separating and purifying the bio-diesel by passing the reaction products into the heater and heating, passing into the flash evaporator for distillation and separating the unreacted lower alcohol to obtain biodiesel.    TECHNOLOGY FOCUS - INSTRUMENTATION AND TESTING - Preferred Components: The fixed bed reactor adopts a tubular structure and the immobilized lipase is filled in the tube. The fixed bed reactor utilizes a constant temperature water bath to maintain water temperature. The outlets of fixed bed reactor and the flash evaporator are connected with the low-carbon alcohol storage tank. The outlet of the liquid-liquid swirler is connected with the glycerol storage tank and the outlet of the flash evaporator is connected with the diesel storage tank. The mixing tank and the fixed bed reactor, the storage tank and the liquid-liquid swirler, the reaction recovery mechanism and the last reaction recovery mechanism with the heater are connected via the high pressure pump.    TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Components: The vegetable fats and oils comprise triglycerides. The lower alcohol is methanol and ethanol.