TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: In increasing triacylglycerol production in the cell, the modifying comprises inhibiting the phosphatidylethanolamine N-methyltransferase (PEMT) pathway in the cell, where the inhibiting comprises reducing the quantity or activity of an enzyme, enzyme cofactor, precursor compound, or intermediate compound of the PEMT pathway. The PEMT pathway enzyme is phosphatidylethanolamine methyltransferase or phospholipid methyltransferase. The inhibiting comprises reducing the quantity or activity of transcription factor that regulates a PEMT pathway protein. The inhibiting comprises reducing the quantity or activity of a protein selected from: inositol-1-phosphate synthase, cytidine diphosphate (CDP)-diacylglycerol synthase, casein kinase II, a beta regulatory subunit of casein kinase II, a protein homologous to Saccharomyces cerevisiae INO2/YDR123C, a protein homologous to S. cerevisiae INO4/YOL108C, a protein homologous t0 S. cerevisiae RTC2/YBR147W, a protein homologous to S. cerevisiae MRPS35/YGR165W, a protein homologous to S. cerevisiae FLD1/YLR404W, and a protein homologous to S. cerevisiae FLD1/YLR404W. The cell is a bacterial cell, a yeast, an algal cell or a plant cell. The triacylglycerol comprises at least one saturated fatty acid chain, and at least one short chain fatty acid having less than 14 carbon atoms. The method comprises inhibiting the expression of a gene in the cell. In producing the genetically modified organism, the genetic modification inhibits the PEMT pathway in the cell. The genetic modification reduces the quantity or activity of an enzyme, enzyme cofactor, precursor compound, or intermediate compound of the PEMT pathway. Also, the genetic modification reduces the quantity or activity of a transcription factor that regulates a PEMT pathway protein. The genetic modification reduces the quantity or activity of a protein selected from: inositol-1-phosphate synthase, CDP-diacylglycerol synthase, casein kinase II, a beta regulatory subunit of casein kinase II, a protein homologous to S. cerevisiae INO2/YDR123C, a protein homologous to S. cerevisiae INO4/YOL108C, a protein homologous to S. cerevisiae RTC2/YBR147W, a protein homologous to S. cerevisiae MRPS35/YGR165W, a protein homologous to S. cerevisiae FLD1/YLR404W, and a protein homologous to S. cerevisiae FLD1/YLR404W. The genetically modified organism is a bacterium, yeast, plant or alga. The genetic modification inhibits the expression of a gene in the cell. The protein comprises a sequence as set forth in any one of fully defined approximately 20-400 amino acids (SEQ ID NOS: 1-36, SEQ ID NO: 49), given in the specification, or a variant of any one of the sequences, or a fragment of any one of the sequences. The gene comprises a sequence as set forth in any one of SEQ ID NOS: 44-47, SEQ ID NOS: 50-52, SEQ ID NO: 54, sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication, a variant of any one of the sequences, or a fragment of any one of the sequences. Preferred Method: In producing the biofuel, the transesterifying is performed using a monohydric alcohol and a base catalyst. EXAMPLE - No suitable example given.