NOVELTY - A recombinant host cell (A1) comprising an isobutanol biosynthetic pathway, is new, where the pathway comprises the substrate to product conversion pyruvate to acetolactate catalyzed by a polypeptide encoded by a heterologous polynucleotide integrated into the chromosome, and where the pathway comprises the substrate to product conversion acetolactate to 2,3-dihydroxyiso valerate catalyzed by a polypeptide encoded by a polynucleotide on a plasmid.    USE - For producing biosynthetic pathway product and fuel composition including an alcohol, particularly isobutanol, 2,3-butanediol, butanol (i.e. 2-butanol), or 2-butanone biosynthetic pathway (claimed).    ADVANTAGE - The recombinant host cell is free of pyruvate decarboxylase activity; increases the copy number or expression of a non-integrated recombinant polynucleotide that catalyzes a step of a biosynthetic pathway; increases the flux in a pyruvate-utilizing biosynthetic pathway; is used as a production host preferably has enhanced tolerance to the produced chemical, and/or can have a high rate of carbohydrate utilization; and increases the formation of product of alcohol i.e. butanol. The rate of butanol production is increased by at least 10 (preferably at least 40, especially at least 70, particularly at least 95)%, or at least 2 (preferably at least 3, especially at least 4)-fold greater as compared to a recombinant host cell where the polynucleotide encoding a polypeptide that catalyzes the conversion of pyruvate to acetolactate is not integrated into the chromosome. The titer of butanol production is increased by at least 10 (preferably at least 40, especially at least 70, particularly at least 95)%, or at least 2 (preferably at least 3, especially at least 4)-fold greater as compared to a recombinant host cell where the polynucleotide encoding a polypeptide that catalyzes the conversion of pyruvate to acetolactate is not integrated into the chromosome.    DETAILED DESCRIPTION - A recombinant host cell (A1) comprising an isobutanol biosynthetic pathway, is new, where the pathway comprises the substrate to product conversion pyruvate to acetolactate catalyzed by a polypeptide encoded by a heterologous polynucleotide integrated into the chromosome, and where the pathway comprises the substrate to product conversion acetolactate to 2,3-dihydroxyiso valerate catalyzed by a polypeptide encoded by a polynucleotide on a plasmid, and where the titer of isobutanol production is increased as compared to a recombinant host cell where the polynucleotide encoding a polypeptide that catalyzes the conversion of pyruvate to acetolactate is not integrated into the chromosome. INDEPENDENT CLAIMS are included for the following:    (1) new recombinant host cell (A2) comprising 2,3-butanediol, 2-butanol, or 2-butanone biosynthetic pathway, where the pathway comprises the substrate to product conversion pyruvate to acetolactate catalyzed by a polypeptide encoded by a heterologous polynucleotide integrated into the chromosome, and where the pathway comprises at least one substrate to product conversion catalyzed by a polypeptide encoded by a polynucleotide on a plasmid;    (2) new recombinant host cell (A3) comprising (a) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of pyruvate to acetolactate where the polynucleotide is integrated into the chromosome, (b) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of acetolactate to 2,3-dihydroxyisovalerate where the polynucleotide is on a plasmid, (c) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of 2,3-dihydroxyiso valerate to alpha -ketoisovalerate, and (d) a polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion of alpha -ketoisovalerate to isobutyraldehyde, where the host cell is free of pyruvate decarboxylase activity, and where the host cell produces isobutanol;    (3) producing (M1) biosynthetic pathway product involving contacting the host cell (A1), (A2) and/or (A3) with a fermentable carbon substrate to form a fermentation broth under conditions where the biosynthetic pathway product is produced;    (4) increasing the copy number or expression of a non-integrated recombinant polynucleotide that catalyzes a step of a biosynthetic pathway, involving contacting the recombinant host cell (A1)-(A3) with a fermentable carbon substrate to form a fermentation broth under conditions where the product of the biosynthetic pathway is produced;    (5) increasing the flux in a pyruvate-utilizing biosynthetic pathway, involving contacting the recombinant host cell (A1), (A2) and/or (A3) host cell with a fermentable carbon substrate to form a fermentation broth under conditions, where the flux in the pyruvate-utilizing biosynthetic pathway in the host cell is increased;    (6) increasing the formation of a product of 2,3-butanediol, isobutanol, 2-butanol, or 2-butanone biosynthetic pathway involving growing the recombinant host cell (A1)-(A3) under conditions where the product of the pyruvate-utilizing pathway is formed, where the amount of product formed by the recombinant host cell is greater than the amount of product formed by a host cell, where the polynucleotide encoding a polypeptide which catalyzes the conversion of pyruvate to acetolactate is not integrated into the chromosome;    (7) a composition comprising the host cell (A1), (A2) and/or (A3), butanol, an extractant, and optionally an esterification enzyme.