TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred System: In the expression system, the genes are regulated artificially by positioning the genes within the nucleotide sequence in a specific order, by including an inducible promoter, by including more than one promoters, or by increasing the copy number of a gene. The nucleotide sequence further includes a gene that encodes a scaffolding protein that is capable of binding to the 1 to 150 enzymes. It also includes a gene encoding a microbial cell surface protein that is capable of binding to the scaffolding protein or is fused with the scaffolding protein. The system further comprises a support capable of binding to the 1 to 150 enzymes, where 1 to 150 enzymes are selected from a cellulose-degrading enzyme, an exoglucanase, an endoglucanase, a cellubiohydrolase, a beta -glucosidase, a xylanase, a lichenase, a mannanase, a chitinase, an endopygalactorunase, a protease, a nuclease, a lipase, a laccase, an amylase, and a beta -galactosidase. Each of the genes encoding the enzymes is from Clostridium thermocellum, Clostridium cellulovorans, Clostridium cellulolyticum, Clostridium papyrosolvens, Trichoderma longibrachiatum, Bacteroides cellulosolvens, Acetivibrio cellulolyticusas, Piromyces spp. 12, or Neocallimastix frontalis. The multi-enzyme complex is a cellulosome. The promoter is a Pr promoter, and the nucleotide sequence includes Clostridium thermocellum genes that are cipA, celK, celS, celR, sdbA, celA, xynC, and xynZ. The order of the genes in the nucleotide sequence is cipA, celK, celS, celR, sdbA, celA, xynC, and xynZ, cipA being proximal to the promoter; or the order of the genes in the nucleotide sequence is cipA, xynZ, xynC, celA, sdbA, celK, celR, and celS, cipA being proximal to the promoter. Cell surface anchoring protein is a cellulosomal surface anchoring protein containing at least one cohesin domain, and the scaffolding protein is a cellulosomal scaffoldin protein containing (a) at least one dockerin domain for binding to the at least one cohesin domain in the surface anchoring protein, and (b) at least one cohesin domain for binding to one of the enzymes. Preferred Cell: In the host cell, the nucleotide sequence further includes a gene encoding a microbial cell surface protein that is capable of binding to the scaffolding protein or is fused with the scaffolding protein. The host cell includes a cellulosome anchored to the surface of the cell. It is a Bacillus subtilis, an Escherichia coli, a Lactobacillus, an algal, or a yeast. Preferred Method: Producing cellusome further comprises collecting a supernatant of the culture and isolating the cellusome from the supernatant using cellulose pellets; and isolating the cells from the culture. EXAMPLE - No suitable example given.