NOVELTY - Culturing high-salinity wastewater phenol degradation transgenic bacteria, involves: using saline-alkali soil as halophilic bacteria source, screening bacteria at specific conditions; using river water to culture bacteria, screening at specific conditions; culturing bacteria in saturated salt water in presence of electric shock, heating, cooling, depositing on polyethylene glycol, retaining supernatant; treating bacteria with calcium chloride solution, obtaining bacterial supernatant fluid, coating on phenol culture medium and culturing; and cultivating on modified phenol culture medium.    USE - For culturing and application of high-salinity wastewater phenol degradation transgenic bacteria (claimed), where wastewater is produced after usage of sea water in electric power, chemical industry, machinery, building, printing, dyeing, or as industrial cooling water or industrial process water; and for sewage treatment.    ADVANTAGE - The high-salinity wastewater phenol degradation transgenic bacteria have strong high-salinity wastewater degradation ability such as are capable of degrading more than 20% salt concentration in phenol waste water, have strong and good phenol removing effect, and high efficiency in sewage treatment. The rate of removing salt form phenol waste water is 99% for salt content of more than 20-25%. Thus method has economic benefit and social benefit, and wide application prospect.    DETAILED DESCRIPTION - Method for culturing and application of high-salinity wastewater phenol degradation transgenic bacteria, involves: using saline-alkali soil as halophilic bacteria source, screening halophilic bacteria at 35-40 degrees C and at pH of 7.5-8.5 on MGM culture medium, for 2-3 days, and keeping halophilic bacteria in cold storage; using river water for culturing bacteria source, screening bacterial culture at 35-37 degrees C, pH of 7-8, for 16-24 hours to obtain phenol degradation bacteria, and keeping phenol degradation bacteria in cold storage; culturing halophilic bacteria in saturated salt water in presence of electric shock for 6-8 hours, then heating to 60-70 degrees C for 3-5 minutes to obtain mixed solution, cooling, depositing on polyethylene glycol (PEG), removing precipitate, and retaining supernatant; treating phenol-degradation bacteria with CaCl2 solution, obtaining phenol degradation bacteria in supernatant fluid, coating phenol culture medium with the bacteria and culturing at 35-37 degrees C for 6-7 days to obtain transgenic bacterial source; and cultivating obtained transgene bacteria after coating on modified phenol culture medium at 35-40 degrees C and pH of 7.5-8.5, for 3-4 days.