TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation: No preparation method is given. Preferred Components: The recombinant microorganism belongs to a genus Escherichia, Saccharomyces or Kluyveromyces. The recombinant microorganism is Escherichia coli. The gene encoding the lactate dehydrogenase (ldh) is ldhA or its homolog or variant. The gene encoding the phosphotransacetylase (pta) is pta or its homolog or variant. The alcohol dehydrogenase (adh) is an ethanol dehydrogenase, where the gene encoding the ethanol dehydrogenase is adhE or its homolog or variant. The recombinant microorganism comprises a gene encoding an enzyme that reduces malonyl-coenzyme A into malonic semialdehyde and a gene encoding an enzyme that reduces malonic semialdehyde into 3-HP, where the gene encoding an enzyme that reduces malonyl-coenzyme A into malonic semialdehyde is a gene encoding a malonyl-coenzyme A reductase, and the gene encoding an enzyme that reduces malonic semialdehyde into 3-HP is a gene encoding a malonate semialdehyde reductase. The recombinant microorganism further comprises a gene encoding an NADPH regeneration enzyme. The gene encoding the NADPH regeneration enzyme is a gene encoding a transhydrogenase or a gene encoding a glyceraldehyde-3-phosphate dehydrogenase, where the gene encoding the transhydrogenase is pyridine nucleotide transhydrogenase or soluble pyridine nucleotide transhydrogenase, and the gene encoding glyceraldehyde-3-phosphate dehydrogenase is non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase. The recombinant microorganism is a microorganism deposited in the Korean Collection for Type Cultures as Deposit No. KCTC11947BP, KCTC11948BP, KCTC11949BP or KCTC11950BP. Preferred Method: The recombinant microorganism has undergone deletion or knockout of the genes encoding lactate dehydrogenase, phosphotransacetylase and alcohol dehydrogenase. The recombinant microorganism reduces or inhibits conversion of pyruvate into lactate, conversion of acetyl-coenzyme A into acetyl phosphate or conversion of acetyl-coenzyme A into ethanol. In the method, the inhibition of the enzyme activity is performed by deletion or knockout of at least one gene comprising of ldhA, pta and adhE in the microorganism. The method is performed by incubating the recombinant microorganism in a medium containing a carbon substrate, where the carbon substrate is at least one glucose, sucrose, cellulose and glycerol. EXAMPLE - No suitable example given.