TECHNOLOGY FOCUS - BIOLOGY - Preferred Components: The transgenic phototrophic organism is plant (preferably Brassica, Gossypium hirsutum, Linum usitatissimum, Helianthus, Carthamus tinctorius, Glycine max, Zea mays, Arabidopsis thaliana, Sorghum bicolor, Sorghum vulgare, Avena sativa, Trifolium, Camelina sativa, Miscanthus x giganteus, or Miscanthus sinensis). The transgenic phototrophic organism is plant, and where the part is seed, fruit, or vegetative part of the plant. The transgenic phototrophic organism or its part further comprises an exogenous glycerol-3-phosphate acyltransferase (GPAT), where the exogenous GPAT has phosphatase activity to produce MAG.    TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: In the method (M1), the level of triacylglycerol (TAG) of the transgenic phototrophic organism or its part is at least 0.5 or 1 wt./wt.% greater on a weight basis than in the organism or its part lacking the exogenous MGAT. The total non-polar lipid content of the transgenic phototrophic organism or its part is at least 0.5 wt./wt.% greater on a weight basis or at least 1 wt./wt.% greater on relative basis than in the organism or its part lacking the exogenous MGAT. The total oil content, or the total fatty acid content, of the seed is at least 0.5-25 wt./wt.% greater on a weight basis than in the organism or its part lacking the exogenous MGAT. The part is vegetative part of plant, and where the TAG, diacylglycerol (DAG), TAG and DAG, or monoacylglycerol (MAG) content of the vegetative plant part is at least 10 wt./wt.% greater on a relative basis than the TAG, DAG, TAG and DAG, or MAG content of the part of the organism lacking the exogenous MGAT. The polyunsaturated fatty acid content of the extracted lipid is increased when compared to the polyunsaturated fatty acid content of the organism or its part lacking the exogenous MGAT. The level of the non-polar lipid is determinable by analysis by using gas chromatography of fatty acid methyl esters obtained from the extracted lipid. The method (M1) further involves harvesting the seed from the transgenic plant and/or pressing seedoil from the seed, and/or purifying seedoil from the seed; reacting the extracted lipid with alcohol, optionally in the presence of catalyst to produce alkyl esters; and blending the alkyl esters with petroleum based fuel. The level of the non-polar lipid in the transgenic phototrophic organism or its part is at least 0.5 wt./wt.% greater on a weight basis and/or at least 1 wt./wt.% greater on a relative basis when compared to such an organism or its part lacking the exogenous MGAT but comprising an exogenous Arabidopsis thaliana thaliana diacylglycerol acyltransferase (DGAT1).    TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Components: The fatty acid content (at least 60 mol%) of the extracted lipid is oleic acid. EXAMPLE - No suitable example given.