NOVELTY - Pyrroloquinoline quinone dependence glucose-dehydrogenase variant having improved reactivity with respect to glucose in high concentration glucose conditions and carried out from wild-type pyrroloquinoline quinone dependence glucose dehydrogenase, comprising: (a) amino acid sequence by which 368-isoleucine is substituted to threonine or alanine in fully defined 478 amino acids (SEQ ID NO. 2) given in the specification; or (b) amino acid sequence by which one or more amino acids are substituted, deleted, added or inserted in locations other than 368-isoleucine in SEQ ID NO. 2, is new.    USE - The variant is useful for preparing fuel cell and glucose sensor; and for detecting glucose (all claimed).    ADVANTAGE - The invention provides pyrroloquinoline quinone dependence glucose-dehydrogenase variant having improved reactivity with respect to glucose in high concentration glucose conditions (claimed).    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are:    (1) an isolated nucleic acid molecule encoding the pyrroloquinoline quinone dependence glucose-dehydrogenase variant;    (2) a recombinant vector comprising the isolated nucleic acid molecule;    (3) a transformant comprising the recombinant vector;    (4) a method for preparing pyrroloquinoline quinone dependence glucose-dehydrogenase variant having improved reactivity with respect to glucose in high concentration glucose conditions;    (5) a fuel cell provided with electrode which is immobilized with the pyrroloquinoline quinone dependence glucose-dehydrogenase variant on electroconductive base material connectable with external circuit;    (6) a method for detecting glucose comprising detecting glucose using catalytic activity of the pyrroloquinoline quinone dependence glucose-dehydrogenase variant; and    (7) a glucose sensor provided with electrode which is immobilized with pyrroloquinoline quinone dependence glucose-dehydrogenase variant on electroconductive base material connectable with external circuit.