NOVELTY - Preparing Hypsizygus marmoreus Bigelow and Flammulina velutipes culture matrix through fungus culture medium waste, comprises collecting and physically processing the fungus culture medium waste by detecting the final auriculariales bag, selecting mildew fungi bag with rancidity, crushing at dampish state, adding modifying agent, adjusting pH, basking or drying, and balancing the nutrition of culture medium by taking e.g. processed fungus waste, adding water, absorbing nutrient solution by nutrient biological polymer substance to reach saturated state, and uniformly mixing.    USE - The method is useful for preparing Hypsizygus marmoreus Bigelow and Flammulina velutipes culture matrix through fungus culture medium waste.    ADVANTAGE - The method is economical, ensures high yield, recycles the waste, and reduces the environmental pollution.    DETAILED DESCRIPTION - Preparing Hypsizygus marmoreus Bigelow and Flammulina velutipes culture matrix through fungus culture medium waste, comprises: (i) collecting and physically processing the fungus culture medium waste by detecting the final auriculariales bag, selecting mildew fungi bag with rancidity, removing the bag of the qualified fungi bag, instantly crushing at dampish state, adding modifying agent during crushing and adjusting the pH value to be 6-7, basking for 2-8 days under sunlight or drying at 65-75 degrees C for 6-12 hours until the moisture content is 10-13%; and (ii) balancing the nutrition of the culture medium by taking (in wt.%) processed fungus waste (65-75), bran or rice bran (10-25), soybean flour (2), corn flour (5-10), white sugar (0.5), calcium super phosphate or potassium phosphate (1-2), gypsum (1-2) and magnesium sulfate (0.5-1), adding water to maintain the moisture content to 55-65%, where the addition amount of water is 140-160% of the total weight of the materials, absorbing nutrient solution by nutrient biological polymer substance (0.3-2.5%) to reach saturated state, and uniformly mixing.