NOVELTY - Manufacturing biodiesel fuel comprises esterifying a fatty acid and manufacturing biodiesel fuel while hydrolyzing the phospholipid in biomass feedstock by processing the biomass feedstock containing phospholipid with an enzyme which shows hydrolysis activity with respect to phospholipid.    USE - The method is useful for manufacturing biodiesel fuel (claimed).    ADVANTAGE - By enzyme-treating the biomass feedstock, biodiesel fuel can be manufactured easily and productively.    DETAILED DESCRIPTION - An INDEPENDENT CLAIM is a method for manufacturing biodiesel fuel comprising esterifies a fatty acid and manufacturing biodiesel fuel while hydrolyzing the phospholipid in biomass feedstock by processing the biomass feedstock containing a phospholipid comprising enzyme A comprising: (a1) polypeptide comprising fully defined 289 amino acids (SEQ ID NO. 1) given in the specification; (a2) polypeptide comprising one or more amino acids that are substituted, inserted, deleted and/or added in SEQ ID NO. 1, and shows hydrolysis activity with respect to phospholipid; and (a3) polypeptide which has the amino acid sequence and at least 75% of homology of SEQ ID NO. 1, and shows hydrolysis activity with respect to phospholipid; and enzyme B comprising: (b1) polypeptide comprising fully defined 412 amino acids (SEQ ID NO. 2) given in the specification; (b2) polypeptide comprising one or more amino acids that are substituted, inserted, deleted and/or added in SEQ ID NO. 2, and shows hydrolysis activity with respect to phospholipid; and (b3) polypeptide which has the amino acid sequence and at least 75% of homology of SEQ ID NO. 2, and shows hydrolysis activity with respect to phospholipid; or a method for manufacturing biodiesel fuel comprising esterifying a fatty acid and manufacturing biodiesel fuel while hydrolyzing the phospholipid in biomass feedstock by processing the biomass feedstock containing phospholipid comprising enzyme C comprising: (c1) polypeptide produced from the microorganisms which have a base sequence of fully defined 816 bp (SEQ ID NO. 3) given in the specification as a polynucleotide; (c2) polypeptide produced from the microorganisms which have a polynucleotide hybridized under stringent conditions with a base sequence complementary to SEQ ID NO. 3; and (c3) polypeptide produced from the microorganisms which have a polynucleotide which has the base sequence and at least 75% of sequence identity of SEQ ID NO. 3; and enzyme D comprising: (d1) polypeptide produced from the microorganisms which have a base sequence of fully define 1239 bp (SEQ ID NO. 4) given in the specification as a polynucleotide; (d2) polypeptide produced from the microorganisms which have a polynucleotide hybridized under stringent conditions with a base sequence complementary to the base sequence of SEQ ID NO. 4; and (d3) polypeptide produced from the microorganisms which have a polynucleotide which has the base sequence and at least 75% of sequence identity of SEQ ID NO. 4.