NOVELTY - Integrated method for producing biofuel, involves performing (a) first and second biotechnical process for producing biofuel and/or starting material for biofuel. The method involves culturing microorganisms capable of producing enzymes, optionally separating supernatant and microbial cells, separating biofuel or starting material, and introducing microbial culture, supernatant or protein enriched fraction of supernatant or diluted supernatant comprising catalytically active enzyme(s) into first and/or second biotechnical process or treating feedstock.    USE - The method is useful for producing biofuel chosen from alcohol(s) preferably ethanol, butanol, isopropanol-butanol-ethanol, and/or acetone-butanol-ethanol, and lipid(s). The lipids and alcohols are used as biofuel, or as component of biofuel or starting material for biofuel production. The biofuel is biodiesel or renewable diesel, gasoline and/or jet fuel. The enzyme preparation is useful in biofuel production, preferably in alcohol production or as source of enzymes in other applications. The integrated process systems are useful for producing lipid and alcohol and enzymes used in alcohol production process, preferably ethanol production (all claimed).    ADVANTAGE - The method enables effective and efficient utilization of hemicellulose for the biofuel production, when compared to existing ethanol production methods which does not utilize pentose sugars efficiently. The method enables production of valuable product used for production of biofuels along with production of enzymes, hence the enzymes are cost-effective. The method reduces need of enzyme treatment before use, preferably stabilization of the enzyme. The integrated bioprocess method for lipid production (enzymatic digestion and fermentation) is cost-effective, as need for separate storing unit for enzyme produced is not required. The culture medium needs minimal processing as aerobic/anaerobic process reduces risk of contamination. The method improves economics of the biofuel production, reduces downstream processing costs including water and enzyme stabilization, decreases transportation, packaging and capital costs, and reduces losses through direct transfer of the enzymes to the second biofuel production process.    DETAILED DESCRIPTION - Integrated method for producing biofuel, involves performing (a) first biotechnical process for producing biofuel and/or starting material for biofuel using microorganism capable of producing enzymes, and (b) second biotechnical process for producing biofuel and/or starting material for biofuel. The method involves culturing the microorganisms capable of producing the enzymes, optionally separating supernatant and microbial cells from the microbial culture, separating biofuel or starting material for biofuel(s) from the culture or microbial cells, and introducing the microbial culture, supernatant or protein enriched fraction of the supernatant or diluted supernatant comprising catalytically active enzyme(s) into the first and/or second biotechnical process, or treating feedstock for the process(es). INDEPENDENT CLAIMS are included for the following:    (1) enzyme preparation obtained by the above-mentioned method; and    (2) an integrated process system comprising processes using lignocellulosic material or its fractions as raw material for lipid and alcohol production, where lipid and/or alcohol production process(es) produce(s) enzymes for alcohol production process, preferably ethanol production process.