NOVELTY - Traditional Chinese medicine comprises: Venenum bufonis extract, where extract is obtained by using ethanol water solution (60-95 vol.%) to backflow extract the medicinal material at 60-90 degrees C, concentrating extract, then separating via silica gel column, using petroleum ether and acetone in volume ratio of 1 to 8:1 to process isocratic elution, collecting part of eluent, filtering, concentrating, decompressing, and drying to obtain mixture containing sum of three bufotalins including resibufogenin, cinobufagin and bufalin (less than 95 wt.% according to dry product).    USE - As traditional Chinese medicine comprising Venenum bufonis extract (claimed), useful for treating tumor, for inhibiting growth of tumor cells and neovascularization, relieving pain, and for producing strengthening function.    ADVANTAGE - The Venenum bufonis extract contains mixture of three bufotalins including resibufogenin, cinobufagin and bufalin in an amount of (less than 95 wt.% according to dry product) or (96-100 wt.%) of the extract; as compared to prior art extracts. The method for preparation of Venenum bufonis extract has higher extraction efficiency, increased purity, is safer and more reliable, solvent used causes less environmental pollution, has simple operation, and is easy for industrial production.    DETAILED DESCRIPTION - A traditional Chinese medicine comprises: Venenum bufonis extract, where the extract is obtained by using ethanol water solution (60-95 vol.%) to backflow extract traditional Chinese medicine Venenum bufonis medicinal material at 60-90 degrees C, concentrating extract, then separating via silica gel column, using petroleum ether and acetone in volume ratio of 1-8:1 to process isocratic elution, collecting part of eluent, filtering, concentrating, decompressing, and drying to obtain mixture containing three bufotalins including resibufogenin, cinobufagin and bufalin; in which the sum of three bufotalins is (less than 95 wt.% according to dry product). In traditional Chinese medicine containing Venenum bufonis extract weight percentage of mixture containing three bufotalins including resibufogenin, cinobufagin and bufalin is (96-100 wt.%) of the extract. An INDEPENDENT CLAIM is included for method for preparation of the traditional Chinese medicine Venenum bufonis extract, involving: using ethanol water solution (60-95 vol.%) to backflow extract the traditional Chinese medicine Venenum bufonis medicinal material at 60-90 degrees C, in which ratio of traditional Chinese medicine Venenum bufonis material and extracting solvent is 1 g : 5-25 ml when extracting, extracting time is 30-90 minutes and extraction is carried out 2-4 times; concentrating extract obtained, then separating via silica gel column by using petroleum ether and acetone at volume ratio of 1-8:1 to process isocratic elution, collecting part of eluent, filtering, concentrating, decompressing and evaporating to obtain mixture containing three bufotalins including resibufogenin, cinobufagin and bufalin; and decompressing and drying mixture for 12-96 hours at 30-50 degrees C to obtain traditional Chinese medicine Venenum bufonis extract containing mixture of three bufotalins.