TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation (Disclosed): The M.thermophila fungal cell is prepared by standard recombinant method. Preferred Composition: The fungal cell is genetically modified to reduce the amount of endogenous glucose oxidase and/or cellobiose dehydrogenase produced by the fungal cell and to increase the production of at least one saccharide hydrolyzing enzyme. The fungal cell is genetically modified for at least partially deleting a gene encoding at least one endogenous glucose and/or cellobiose oxidizing enzyme. The fungal cell is genetically modified to reduce the amount of glucose and/or cellobiose oxidizing enzyme activity of two or more endogenous glucose and/or cellobiose oxidizing enzymes that are produced by the fungal cell before genetic modification. Preferred Enzyme: The glucose and/or cellobiose oxidizing enzyme is cellobiose dehydrogenase (EC 1.1.99.18), glucose oxidase (EC 1.1.3.4), pyranose oxidase (EC1.1.3.10), glucooligosaccharide oxidase (EC 1.1.99.B3), pyranose dehydrogenase (EC 1.1.99.29), and glucose dehydrogenase (EC 1.1.99.10). The first and second two or more glucose and/or cellobiose oxidizing enzyme comprises an amino acid sequence exhibiting at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence homology to sequence comprising a 647, 655, 828, 787, 629, 499, 594, or 669 amino acid sequence (SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, or 16) fully defined in the specification. The enzyme mixture is a cell-free mixture comprising at least cellulase enzyme chosen from endoglucanases (EGs), beta -glucosidases (BGLs), type 1 cellobiohydrolases (CBH1s), type 2 cellobiohydrolases (CBH2s), and/or glycoside hydrolase 61s (GH61s), and/or variants of the cellulase enzyme, preferably beta -glucosidase. Preferred Components: The cellulose comprises pretreated lignocellulose. At least one fermentable sugar comprises glucose and cellobiose. The enzyme mixture comprises at least one inhibitor of two or more cellulose hydrolyzing enzymes. Preferred Method: The method further involves performing fermentation of the at least one fermentable sugar to at least one end product. The method involves performing reaction at a reaction volume of at least 10000 liters. The method involves performing production of at least one fermentable sugar, and the fermentation of at least one fermentable sugar by simultaneous saccharification and fermentation process. EXAMPLE - No suitable example given.