NOVELTY - Highly efficient degradation of multiple organic pollutants in environment by green fluorescent protein (GFP) marked microorganism includes conducting double-enzyme cleavage to carrier plasmid pGEM-GFP and carrier pK18; obtaining carrier plasmid pK-GFP-2; transforming into colon Bacillus; amplifying and extracting plasmid; enzyme cutting; connecting the GFP fragment amplification product with enzyme cut pTOPO17 beta -hydroxysteroid dehydrogenase (HSD)-600-3; electrically transforming into cytidylyltransferase (CT) competent cell; and screening.    USE - Highly efficient degradation of multiple organic pollutants in environment by GFP marked microorganism (claimed).    ADVANTAGE - The method is capable of fluorescent tracking in the process of degrading pollutant.    DETAILED DESCRIPTION - Highly efficient degradation of multiple organic pollutants in environment by GFP marked microorganism comprises conducting double-enzyme cleavage to carrier plasmid pGEM-GFP and carrier pK18; connecting to obtain carrier plasmid pK-GFP-2; transforming into colon Bacillus; amplifying and extracting plasmid; enzyme cutting to obtain GFP section; connecting the GFP fragment amplification product with enzyme cut pTOPO17 beta -hydroxysteroid dehydrogenase (HSD)-600-3 to obtain the recombinant plasmid i.e. pTOPO17 beta -600-GFP; electrically transforming into CT competent cell; screening to obtain modified strain CT17 beta -GFP, where T17 genome DNA with GFP gene has a defined sequence of 717 bp (SEQ ID NO: 1), given in the specification, genome DNA containing pK18 gene has a defined sequence of 2661 bp (SEQ ID NO: 2), given in the specification, the CT key enzyme 17 beta -HSD/carbonyl 17 beta -HSD gene has a defined sequence of 600 bp (SEQ ID NO: 3), given in the specification, and CT17 beta -GFP gene is provided with kanamycin and ampicillin resistance; transferring to 0.05-1.0 mM organic pollutant low-nutrient culture medium at 1:50 ratio; continuously shaking culture at a constant temperature of 27 degrees C; and degrading the organic pollutant.