TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation (Claimed): The insecticidal lipid or lipid fraction is prepared by providing organic solvent extraction of a culture of one or more fungi of the phylum Ascomycota, at least partially separating one or more non-acidic lipids from one or more acidic lipids, and recovering the one or more non-acidic lipids or lipid fractions. Preferred Components: The lipid or lipid fraction comprises non-acidic lipids and non-acidic lipid fractions. The lipid fraction is substantially free of acidic lipids or free fatty acids. The lipid fraction has identifying characteristics of (a) fraction 1, fraction 5, or fraction 6 provided in figure 4 of patent specification, (b) fraction 2, fraction 4, or fraction 5 provided in figure 7 of patent specification, (c) fraction 2, fraction 3, fraction 4, fraction 5, or fraction 9 provided in figure 8 of patent specification, (d) fraction 2, fraction 3, fraction 4, fraction 5, or fraction 9 provided in figure 8 of patent specification, and (e) fraction 2, fraction 3, fraction 4b, fraction 5a, fraction 5b, or fraction 6 provided in figure 10 of patent specification. The identifying characteristic of fraction 2, fraction 3, fraction 4b, fraction 5a, fraction 5b, or fraction 6 is matrix-assisted laser desorption/ionization/time-of-flight (MALDI-TOF) mass spectrometry (MS) profile provided in figure 12a-f of patent specification. The lipid fraction has the identifying characteristics of fraction 3 provided in figure 10 of patent specification, and nuclear magnetic resonance (NMR) spectrum provided in figure 13 of patent specification. The lipid is chosen from lipid having an approximate mass at mass/charge (m/z) 86, 147, 445, 656, 454, 751, 393, 751, 693, 695, 717, 531, or 86.02 by MS/MS, and lipid having a mass at m/z 146.97, 444.82, 655.94, 454.19, 750.66, 393.39, 750.67, 692.69, 694.72, 716.71, or 531.34 by MS/MS. The lipid fraction is chosen from lipid having an approximate mass at m/z 86, 147, 445, 656, 454, 71, 393, 751, 693, 695, 717, or 531 by MS/MS, and lipid having a mass at m/z 86.02, 146.97, 444.82, 655.94, 454.19, 750.66, 393.39, 750.67, 692.69, 694.72, 716.71, or 531.34 by MS/MS. The fungus and spores of phylum Ascomycota is chosen from Beauveria bassiana strain K4B3 deposited as national measurement institute of australia (NMIA) No. V08/025855, or its strain having the identifying characteristics, B.bassiana strain AM2 or its strain having the identifying characteristics, B.bassiana strain F480 or its strain having the identifying characteristics, one or more Trichoderma including Trichoderma isolate 1328 or its strain having the identifying characteristics and one or more Metarhizium. Preferred Composition: The composition comprises culture, cell extract, cell suspension, cell homogenate, cell lysate, cell supernatant, cell filtrate, cell pellet, and growth media from culture of fungus of phylum Ascomycota. The composition further comprises spores obtainable from fungus of phylum Ascomycota. The composition comprises one or more lipids or lipid fractions from B.bassiana strain K4B3. The composition has enrichment of at least about 1%, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 99% relative to the amount present in composition without enrichment. ACTIVITY - Insecticide; Fungicide. Test details are described but no results given.    MECHANISM OF ACTION - None given.    ADMINISTRATION - The composition is applied to plant at a dose of 10 kg/hectare.    EXAMPLE - No suitable example given.