TECHNOLOGY FOCUS - BIOLOGY - Preferred Method: The lignocellulosic biomass has been pretreated under conditions to promote accessibility of celluloses within the lignocellulosic biomass. The lignocellulosic biomass is incubated with the extract before being pretreated under conditions to promote accessibility of celluloses within the lignocellulosic biomass. The plant biomass has not been pretreated under conditions to promote accessibility of celluloses within the lignocellulosic biomass. The plant biomass is obtained from a monocotyledonous plant or dicotyledonous plant. The plant biomass is obtained from a plant that comprises at least one soluble component that promotes deconstruction of plant cell wells. The plant biomass is freshly harvested. The plant biomass has been subject to a process comprising grinding, chopping, milling, ensilement, baling, binning, ginning, squeezing, pressing, composting, bagging, drying and/or tempering. The extract is obtained by a process comprising: hydrating the plant biomass in a liquid buffer or subjecting the plant biomass to a procedure comprising rinsing, crushing, chopping, grinding, pureeing, pressing, extruding, milling and/or straining, and separating solids from liquid, thus obtaining the liquid as an extract. The process is performed at a pH 4.5-5.5 and a temperature 15-50 degrees C. The process further comprises: a period of hydration, where the period of hydration extends up to five days; concentrating to reduce volume of liquid extract, diluting to increase the volume of liquid extract or drying to produce a solid extract; removing soluble low molecular mass components from soluble high molecular mass components, thus obtaining an extract that is enriched for enzyme activity; and reducing the content of organic acids. The step of removing is obtained by a fractionation and/or filtration method. The organic acids that are reduced comprise lactic acid. The lignocellulosic biomass is incubated with an enzyme or enzyme cocktail in addition to being incubated with the extract. The extract is mixed with or added to an enzyme or enzyme cocktail during or prior to enzymatic hydrolysis to convert glucans in the lignocellulosic biomass. The starch is obtained from grain from maize, Sorghum, rice, oat, barley, potato, or wheat. Preferred Components: The lignocellulosic biomass is from: the same source as the plant biomass from which plant extract is obtained; the same species of plant as the plant biomass from which plant extract is obtained or a different species of plant as the plant biomass from which plant extract is obtained. The plant biomass is dried distillers grains with solubles. The plant biomass is a byproduct or waste product, where the byproduct or waste product is sawdust, cotton gin waste or grass clippings. The extract is existing plant steepage, where the plant steepage is corn steep water, corn steep liquor, barley steep extract, rice steep extract or wheat steep extract. The plant biomass comprises a plant part comprising leaves, stems, culms, roots, seeds, grain, flowers, fruits, husks, hulls, straw, bark, stover, fiber and/or cobs. The monocotyledonous plant is maize, Sorghum, switchgrass, Miscanthus, sugarcane, wheat, rice, rye, turfgrass, oat, Fescue, millet or grass species. The dicotyledonous plant is tobacco, tomato, sugar beet, potato, soybean, canola, sunflower, alfalfa, cotton, flax, pine, willow, Eucalyptus, Poplar or tree species. The soluble component is polypeptides (preferred), metabolites, chemicals or co-factors. The polypeptide is an enzyme polypeptide, where the enzyme polypeptide is an auto lytic enzyme polypeptide. The enzyme cocktail is ACCELLERASE (RTM: Enzyme complex intended specifically for the lignocellulosic biomass processing industries), CELLIC CTec2 (RTM: Cost-effective cellulase complex with a proprietary hydrolysis-boosting protein that facilitates higher conversion yields) and/or CELLIC? HTec2 (RTM: Concentrated hemicellulase ). The cellulosic material is wood pulp. EXAMPLE - Poplar biomass material (leaves and stems) from wild type Poplar was dried and milled to pass a 20 mesh screen. A subsample (1 g) of the milled material was then incubated with 0.05 M sodium citrate buffer (10 ml, pH 5) for 10 minutes at room temperature. After incubation, the resulting extract solution was separated from the solid by filtering through cheesecloth. A 5 ml aliquot of the extract solution was added to 2 g (wet weight) of dilute acid-pretreated (using conditions sulfuric acid ((0.5%), 190 degrees C for 10 minutes) Poplar with ACCELLERASE 1500 (RTM: Enzyme complex intended specifically for the lignocellulosic biomass processing industries) at a rate of glucan (0.2 ml/g). 2% sodium azide (100 l) was added for microbial inhibition. Reactions were brought to a final volume of 10 ml with distilled water. Slurries were then incubated for 72 hours at 50 degrees C. After incubation, soluble glucose in the solution was measured colorimetrically using the glucose oxidase method.