NOVELTY - Producing enzymes, comprises: cultivating microorganism capable of producing both single cell oil and enzymes under conditions suitable for single cell oil production and enzyme production in a single cell oil production process, and producing single cell oil and enzymes; obtaining microorganism culture comprising single cell oil and enzymes, and recovering at least part of (i) the microorganism culture, of (ii) the supernatant and/or microorganism cells separated from the microorganism culture, of (iii) protein fraction; and recovering single cell oil from the microorganism cells.    USE - The process is useful for producing enzymes which is useful: as a biofuel, component of biofuel or as a starting material for biofuel production, where the biofuel is biodiesel or renewable diesel, gasoline and/or jet fuel; and in industrial process (all claimed).    ADVANTAGE - The process is economic by reducing the energy cost of treatment of lignocellulosic materials or materials comprising other polymeric carbohydrate biomass by using enzymes from the single cell oil process itself in place of commercial enzymes and thermo-mechanical and chemical treatments; reduces the environmental burden caused by large-scale single-cell oil production; reduces the biological oxygen consumption load of the fermentation liquor released from single cell oil production; and improves the carbon balance of a single cell oil process.    DETAILED DESCRIPTION - Producing enzymes, comprises: cultivating a microorganism capable of producing both single cell oil and enzymes under conditions suitable for single cell oil production and enzyme production in a single cell oil production process, and producing single cell oil and enzymes by the microorganisms; obtaining a microorganism culture comprising single cell oil and enzymes, and recovering at least part of (i) the microorganism culture, of (ii) the supernatant and/or microorganism cells separated from the microorganism culture, of (iii) protein fraction enriched from the supernatant, and/or of (iv) protein fraction from the cells, for use as an enzyme preparation or as a source of enzymes; and recovering single cell oil from the microorganism cells. An INDEPENDENT CLAIM is also included for an enzyme preparation obtained by the process.