TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Method: In the step (a), the purified biomass is adjusted to a dry matter range of 6-12%, preferably 10-12%. The step (b) takes place with stirring and without shearing and little emulsification in a device equipped with a marine propeller and baffle. The step (b) takes place at: a temperature of greater than 50 degrees C, preferably 60 degrees C and a pH of greater than 7, preferably 8. In the method, greater than 5, preferably 10 vol./vol.% of ethanol is added at the end of the step (b). The ethanol treatment takes place under stirring for 10 minutes, preferably 15 minutes. The reaction mixture is heated to 60-80 degrees C, preferably 80 degrees C, before the step (c). The step (c) separates three concentration modes, which extracts the upper light (oil) phase from the aqueous phase and the cellular debris. The step (c) takes place with a centrifugal force of greater than 4000, preferably 10000 g. EXAMPLE - Schizochytrium sp. was first precultured in 500 ml Erlenmeyer flasks equipped with baffles. Clearol FBA 3107 (RTM: Antifoaming agent) (one drop) was added. The culture medium (comprising glucose (3%), yeast extracts (0.4%), sodium glutamic acid (6.42%), sodium chloride (1.25%), magnesium sulfate (0.4%), potassium chloride (0.05%), calcium chloride (0.01%), sodium bicarbonate (0.05%), potassium dihyrogen phosphate (0.4%), vitamin mixture (comprising B1 (45 g/l), B6 (45 g/l) and B12 (0.25 g/l)) (0.14%) and trace elements (0.8%) (comprising magnesium chloride dihydrate (8.60 g/l), cobalt chloride hexahydrate (0.2 g/l), nickel sulfate hexahydrate (7.50 g/l), sodium molybdate (VI) dihydrate(0.15 g/l), zinc sulfate heptahydrate (5.70 g/l), copper sulfate pentahydrate (6.50 g/l), ferrous sulfate heptahydrate (32 g/l) and zinc chloride (1.50 g/l))) were filtered after complete dissolution of its constituents. Penicillin G sodium salt (0.25 mg/l) was added. The inoculation was performed by removing colonies of microalgae grown in Petri dishes (at a loopful of 10 feet). The incubation lasted 24-36 hours at a temperature of 28 degrees C under stirring at 100 revolutions per minute. Biomass was decanted to collect 3-5 ml. Second preculture was carried out in 2 ml Erlenmeyer flasks equipped with baffles and pipe. Antifoaming agent (one drop) and yeast extract (one drop) in 100 ml of water was added. All the constituents of the medium (comprising glucose (8.57%), yeast extracts (0.64%), sodium glutamic acid (6.42%), sodium chloride (2%), magnesium sulfate (2.29%), calcium chloride (0.03%), sodium bicarbonate (0.03%), potassium dihydrogen phosphate (0.64%), sodium sulfate (0.03), vitamin mixture (0.14%) and trace elements (0.2%)) was filtered after dissolution in 300 ml of demineralized water. Penicillin G sodium salt (0.25 mg/l) was added. Seeding was done with 3-5 ml of the first preculture. The incubation was performed at 28 degrees C for 24-36 hours under stirring at 100 revolutions per minute. The culture was carried out in a 20 l reactor and the medium was sterilized to obtain biomass of Schizochytrium sp.. The biomass of Schizochytrium sp.was concentrated to 54 g/liter at the end of fermentation. Further, alcalase was added up to 1%/second for 4 hours. At the end of lysis, 10% ethanol was added, stirred for 15 minutes and the temperature was increased to 80 degrees C. The obtained mixture was centrifuged at 9600 revolutions per minute to obtain crude oil containing 85% of squalene.