NOVELTY - The method comprises taking Eupatorium odoratum L., drying and grinding the Eupatorium odoratum L. at 50-60 degrees C, mixing 1 g of ground Eupatorium odoratum L. and 15-25 ml of edible ethanol with volume concentration of 50-60%, extracting the mixture using ultrasonic assisted extraction process at 120-200 W and 20-25 degrees C for 1-2 hours, sieving the extract with 200 meshes of nylon cloth, centrifuging the mixture at 3500-4500 revolutions per minute for 5-15 minute, taking the supernatant, and concentrating supernatant at -0.05 MPa to -0.09 MPa and 50-60 degrees C.    USE - The method is useful for separating and purifying Eupatorium odoratum L. flavonoids through HZ816 macroporous adsorption resin.    ADVANTAGE - The method is capable of easily, economically and safely separating and purifying the Eupatorium odoratum L. flavonoids with high reliability and efficiency in an environmentally friendly manner.    DETAILED DESCRIPTION - The method comprises taking Eupatorium odoratum L., drying and grinding the Eupatorium odoratum L. at 50-60 degrees C, mixing 1 g of ground Eupatorium odoratum L. and 15-25 ml of edible ethanol with volume concentration of 50-60%, extracting the mixture using ultrasonic assisted extraction process at 120-200 W and 20-25 degrees C for 1-2 hours, sieving the extract with 200 meshes of nylon cloth, centrifuging the mixture at 3500-4500 revolutions per minute for 5-15 minute, taking the supernatant, concentrating the supernatant at pressure of -0.05 to -0.09 MPa and 50-60 degrees C, recycling the edible ethanol, drying the product under vacuum to obtain a crude extract, preparing the flavonoids crude extract to flavonoid solution with concentration of 2.75-3.25 mg/mL, taking 7-9 BV of flavonoid solution through a chromatographic column with HZ816 macroporous adsorption resin with flow rate of 2.5-3.5 BV/hour at room temperature, making the adsorption resin to saturation, washing with 4-6 BV of distilled water to become colorless with flow rate of 1-3 BV/hour, washing out the flavonoids with 5-7 BV of edible ethanol with volume concentration of 50-70% at flow rate of 1.5-2.5 BV/hour, collecting the eluent, concentrating the eluent under the condition of -0.05 to -0.09 MPa and 50-60 degrees C, recycling the edible ethanol, and drying the product under vacuum to obtain the extract of Eupatorium odoratum L. flavonoids.