NOVELTY - A raw material for enzyme saccharification is manufactured by ammonia treatment process which disconnects part(s) of ester bond in biomass feedstock containing lignin, cellulose and hemicellulose and processes with ammonia, and wet-grinding process which wet-pulverizes biomass by which ammoniation is carried out. The water content ratio of dry mass (A) of biomass and mass (B) of water in ammonia treatment process is less than or equal to 30. The disconnection of ester bond is carried out until ester bond persistence is less than or equal to 90%.    USE - Manufacture of raw material for enzyme saccharification for manufacturing sugar used for manufacturing ethanol (claimed) for use as ethanol for fuels and industrial ethanol. Can also be used for manufacture of lactic acid, and manufacture of environment-friendly biodegradable plastics.    ADVANTAGE - The enzyme saccharification is efficiently performed. The productive efficiency is improved.    DETAILED DESCRIPTION - A raw material for enzyme saccharification is manufactured by ammonia treatment process which disconnects part(s) of ester bond in biomass feedstock containing lignin, cellulose and hemicellulose and processes with ammonia, and wet-grinding process which wet-pulverizes biomass by which ammoniation is carried out. The water content ratio ((B divide by (A+B))x 100) of dry mass (A) of biomass and mass (B) of water in ammonia treatment process is less than or equal to 30. The disconnection of ester bond is carried out until ester bond persistence calculated by formula ester bond persistence (%)=(ester bond peak of biomass by which ammoniation is carried out/ester bond peak of biomass feedstock)x 100 in which ester bond peak is value of ester bond produced by condensation with fatty acid and aliphatic alcohol in light absorption wavenumber of 1730 cm-1 vicinity measured by Fourier transform infrared spectrophotometer obtained by dividing area of absorption peak which belongs to lignin conjugation carbonyl (C=O) stretching vibration with respect to area of absorption peak which belongs to benzene nucleus structure vibration (C=C) of light absorption wavenumber of 1515 cm-1 vicinity is less than or equal to 90%. INDEPENDENT CLAIMS are included for:    (1) manufacturing method of sugar involving carrying out enzyme saccharification of raw material for enzyme saccharification and obtaining sugar; and    (2) manufacturing method of ethanol involving fermenting sugar and obtaining ethanol.