NOVELTY - Preparing inactivated vaccine, comprises: e.g. (1) generating and cultivating the cell for preparing vaccine by digesting and generating the cell with the EDTA pancreatin cell dispersing liquid and then cultivating in a cell culture bottle or bioreactor with cell growing liquid and forming the cultivating cell; and (2) domesticating the vaccine for adapting cell by diluting the avian influenza virus to the density of 0.1-5 wt.% virus liquid with a cell maintenance media, and inoculating the virus liquid into a cultivating plate grew into monolayer cell.    USE - The method is useful for preparing inactivated vaccine, which is: H9 subtype and F avian influenza inactivated vaccine; and useful for treating avian influenza virus (claimed).    ADVANTAGE - The method is capable of rapidly, safely and economically preparing the inactivated vaccine with high efficiency in an environmentally friendly manner.    DETAILED DESCRIPTION - Preparing inactivated vaccine, comprises: (1) generating and cultivating the cell for preparing vaccine by digesting and generating the cell with the EDTA pancreatin cell dispersing liquid and then cultivating in a cell culture bottle or bioreactor with cell growing liquid and forming the cultivating cell; (2) domesticating the vaccine for adapting cell by diluting the avian influenza virus to the density of 0.1-5 wt.% virus liquid with a cell maintenance media, inoculating the virus liquid into a cultivating plate grew into monolayer cell, adding tosyl phenylalanyl chloromethyl ketone (TPCK) pancreatin and then adsorbing for 0.5-3 hours in an incubator, adding cell maintenance media and then putting into a cultivating box for cultivating, and continuously passing to make the vaccine adapt to the cell to obtain domesticated avian influenza producing virus seed; (3) optionally setting up the virus seed group by taking the low-passage seed as a primitive seed, base seed and/or the production seed; (4) cultivating the cell for preparing vaccine by inoculating the culture cell into the domesticated avian influenza producing virus seed and then adding TPCK pancreatin to adsorb for 0.5-3 hours, and adding cell maintenance media for continuously cultivating; (5) harvesting the cell for preparing vaccine by sampling the cell for detecting the left glucose content and virus liquid blood coagulation value in the medium after cultivating for 10-50 hours, harvesting the virus liquid as the two estimated value tend to stable; and (6) optionally producing the vaccine product by centrifugally separating the harvested virus liquid and then inactivating, and emulsifying and producing the vaccine end product.