NOVELTY - Separating neutral lipids from intact algal cells involves: (A) providing wet algal biomass; (B) optionally dewatering; (C) mixing with first amphipathic and hydrophobic solvents to form heavier phase of amphipathic solvent, neutral lipids, proteins and biomass, and lighter hydrophobic solvent; (D) separating; (E) separating biomass comprising neutral lipids and proteins; (F) mixing biomass with second amphipathic and hydrophobic solvents to form heavier phase of amphipathic solvent and biomass, and lighter hydrophobic solvent; (G) separating; and (H) separating neutral lipids from lighter phase.    USE - For separating neutral lipids from intact algal cells (claimed) useful as e.g. renewable fuels.    ADVANTAGE - The method provides improved method for extracting and fractionating algal products by utilizing step extraction and fractionation with amphipathic and hydrophobic solvents to process wet algal biomass so as to separate neutral lipids, and further produces oils, proteins, and polar lipids. The method allows for separation and fractionation of components of wet algal biomass. Further, method has ability to accomplish preliminary refining, because hydrophobic solvent in system selectively separates products further into lipids and non-lipid components. The amount of hydrophobic solvent is minimized in order to reduce later processing to remove solvent. The method thus allows for simultaneous extraction and fractionation of algal products from algal biomass in a manner that allows for production of both fuels and nutritional products. The introducing of highly non-polar solvent to extraction system avoids cost of evaporating and recycling all of solvent used for extraction. The method selectively removes products from algal biomass comprising intact algal cells.    DETAILED DESCRIPTION - Method for separating neutral lipids from intact algal cells, involves:    (A) providing a wet algal biomass comprising intact algal cells which comprise proteins, polar lipids, and neutral lipids;    (B) optionally dewatering the wet algal biomass by removing extracellular water to increase the solid content of the wet algal biomass to 5-50% to generate a partially dewatered wet algal biomass;    (C) mixing the partially dewatered wet algal biomass or wet algal biomass with a first amphipathic solvent set and a first hydrophobic solvent set to generate a first extraction mixture comprising a first heavier phase and a first lighter phase, where the first heavier phase comprises the first amphipathic solvent set, neutral lipids, proteins, and partially dewatered wet algal biomass or wet algal biomass, and the first lighter phase comprises the first hydrophobic solvent set and the polar lipids;    (D) separating the first lighter phase of the extraction mixture from the first heavier phase of the extraction mixture;    (E) separating the partially dewatered wet algal biomass or wet algal biomass from the first amphipathic solvent set in the first heavier phase of the extraction mixture to generate a separated partially dewatered wet algal biomass or wet algal biomass comprising neutral lipids, and a protein mixture comprising proteins, water and the first amphipathic solvent set;    (F) mixing the separated partially dewatered algal biomass or wet algal biomass with a second amphipathic solvent set and a second hydrophobic solvent set to generate a second extraction mixture comprising a second heavier phase and a second lighter phase, where the second heavier phase comprises the second amphipathic solvent set and separated partially dewatered wet algal biomass or wet algal biomass, and the second lighter phase comprises the second hydrophobic solvent set and the neutral lipids;    (G) separating the second heavier phase from the second lighter phase; and    (H) separating the neutral lipids from the second lighter phase.