TECHNOLOGY FOCUS - BIOLOGY - Preferred Components: The lignocellulose raw material comprises corn straw, corn core, or wheat straw and branches. The Clostridium pasteurianum strain SE-6 (CCTCC NO: M 2012109. Preferred Method: The wet milling process is carried out by: (a) grinding lignocellulose raw material using a grinding disc, where the gap between the upper and lower grinding disc is controlled at 2-4 mu m; and (b) wet-grinding the pre-treated raw material, and carrying out particle size analysis of pre-treated raw material by using a laser particle size analyzer, and stopping the grinding process when more than 90% of the raw materials subjected to wet grinding has a particle diameter of 20-60 mu m, and carrying out enzymatic hydrolysis by using cellulase. The enzymatic hydrolysis by cellulase is carried out by adding 16 filter paper unit (FPU) Acremonium hansfordii complex cellulose/g of dry matter, where the amount of dry matter in the enzymolysis system accounts to 10%, uniformly mixing at 50 degrees C at 200 rotations/minute, and subjecting to hydrolysis for 48 hours. The butanol fermentation is carried out by taking crude glycerine by-product raw material obtained by trans-esterification of biodiesel, and subjecting the non-purified crude glycerine to purification treatment. The Clostridium pasteurianum strain SE-6 is fermented by using biodiesel by-product crude glycerine for producing butanol, and controlling the fermentation temperature at 30-35 degrees C, and pH at 6-6.5. The method further comprises: (c) pre-treating lignocellulose by crushing, and wet-grinding, and preparing saccharified liquid by carrying out hydrolysis using cellulose when the when more than 90% of the raw material subjected to wet grinding has particle diameter of 20-60 mu m; (d) producing microbial oils and fats by carrying out fermentation using lignocellulose hydrolysis solution as carbon source, and Candida humicola (ATCC NO: 96219); (e) centrifuging by using a tubular centrifuge, and collecting the bacterial cells, crushing the bacterial cells by heat method, and recovering the microbial oil by using an organic solvent; (f) preparing biodiesel oil and glycerol by trans-esterification reaction of the microbial oil; (g) carrying out butanol fermentation by adding glycerol (10-60 g/l), carrying out steam sterilizing at 121 degrees C for 30 minutes, cooling to room temperature, adding ferrous sulfate by sterile filtering, and adding to butanol fermentation culture medium; and (h) automatically controlling the temperature at 30-35 degrees C, controlling the pH at 6-6.5, and simultaneously introducing nitrogen, removing oxygen from a fermentation tank, and adding Clostridium pasteurianum strain SE-6 before carrying out fermentation, and carrying out butanol fermentation.