TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Method: The step (b) and (b1) comprise agitating the mixture for at least 1 hour at 30-60 degrees C. The step (c) and (c1) comprise discontinuing the agitating and decanting at least a portion of the aqueous solution or the first aqueous solution from the insoluble portion comprising settled solids. The step (a) comprises milling the lignocellulosic material to a size of less than 2 mm. The step (d) comprises milling or grinding the first insoluble portion to a size of less than 50 mu . The method (II) further comprises: washing the first insoluble portion at least once after the step (c1) and drying the washed first insoluble portion before the step (d); and bio-fermentation processing of the 6C and/or 5C sugar. Preferred Components: The aqueous solution or the first aqueous solution comprise an aqueous acidic organic buffer solution. The 6C and/or 5C sugar comprises solubilized and/or suspended 6C and/or 5C sugar. The enzyme blend comprises: a first enzyme blend comprising at least one enzyme comprising at least two of (a) endoglucanase and carboxymethylcellulase, (b) exoglucanase and avicelase, (c) cellobiase, beta -glucosidase and alpha -glucosidase, (d) endo-1,4- beta -xylanase and endo-1,4- beta xylanohydrolase or (e) beta -1,3-xylanase, 1,3- beta -D-xylosidase and exo-1,3- beta -xylosidase; and a second enzyme blend comprising at least one enzyme comprising at least two of endo-1,3(4)- beta -glucanase, laminarinase (endo-1,3- beta -glucanase), exo-1,2-1,6- alpha -mannosidase, beta -D-xylopyranosyl-(1,4)- beta -D-xylopyranose, alpha -N-arabinofuranosidase, feruloyl esterase, endo-1,5- alpha -arabinanase, pectinase, polygalacturonase, pectin esterase, aspartic protease, metalloprotease, endo-1,4-mannanase, phytase, alpha -glucuronidase, beta -glucuronidase, hexenuronidase, alkaline phosphatase, acid phosphatase, alpha -galactosidase, beta -galactosidase, beta -mannosidase or alpha -fucosidase. The system further comprises: a dryer for drying the insoluble portion comprising lignin; a comminutor (A2) for comminuting the first insoluble material; a digester (D2) comprising a vessel equipped with an internal agitator, where the vessel is operable for holding a mixture of the comminuted first insoluble material, the enzyme and aqueous solution, and contacting the mixture with agitation for enzymatic hydrolysis; a separator (S2) for separating the portion of the aqueous solution from the insoluble portion; and a bio-fermentation processing system adapted to receive the liberated 6C and/or 5C sugar from the digester and ferment the sugar into ethanol. EXAMPLE - Cotton seed hulls were dry milled to sizes of less than 2 mm and the milled cotton seed hulls ( approximately 220 g) was made up as a 10% solids solution in citrate buffer (50 mM) of pH 5. The temperature of the 10% cotton seed hull solution was raised to approximately 50 degrees C and held at that temperature. The heated solution was amended with first enzyme solution (comprising a mixture of enzymes comprised of Cellic CTEC (RTM: Enzyme preparation including endoglucanases, cellobiohydrolases and beta -glucosidases) (1/3 parts by weight), glucosidases (1/3 parts by weight) and xylanase (1/3 parts by weight)) (0.5%) in a fermentation beaker (4 l). The resulting mixture was agitated at approximately 200 rpm for 24 hours. The agitation was discontinued and solids were allowed to settle. The aqueous phase containing sugars and suspended fine sugar particulates above the settled solids was decanted from the digester. The settled solids were resuspended in the digester by addition of water and acidified with hydrochloric acid to a pH 2.5 and agitated. Agitation was discontinued after washing the solids and the solids were allowed to settle again. The aqueous phase of wash fluid was decanted from the digester leaving only solids. The solids were resuspended in water, agitated and recollected after discontinuing agitation. The solids were dried in a hot air oven at approximately 50 degrees C overnight. The dried precipitate was milled to less than 50 mu in size. The milled powder was made up to a 10% solids solution in citrate buffer (0.05 mM) of pH 5. The solution was agitated at approximately 200 rpm and amended with a second enzyme solution (comprising cellulase (1/3 parts by weight) and penicillium ferment (2/3 parts by weight)). The resulting mixture was digested at approximately 45 degrees C for 6 hours. Agitation was discontinued and solids were allowed to settle. The aqueous phase above the settled solids was decanted, removing as much fine particulate suspended in the aqueous phase as possible while leaving the heavier settled solids in the digester. The precipitate was dried in the hot air oven and a finished dried material comprising lignin was obtained.