TECHNOLOGY FOCUS - BIOLOGY - Preferred Method: The cultivating cell in step (1) is within 40 generations. The cell growing liquid in step (1) is the Dulbecco's Modified eagle media (DMEM) medium containing 8-10 wt.% of serous, and the DMEM medium containing 3-5 wt.% of the serous or medium without serous. The cultivating condition in step (1) is carried out with the dissolved oxygen content of 30-60%, the pH of 7-7.3, the temperature of 36.5-37.5 degrees C, and the turning speed of the reactor of 40-50 revolutions per minute. The reactor is auto automatically controlled. The cell is cultivated via suspending method. The TPCK pancreatin is added in step (2) until the density of the TPCK pancreatin be 0.1-3 mu g/ml. The cultivating condition in step (2) is carried out with the pH of 7-7.2, the temperature of 36-37 degrees C, and the density of the carbon dioxide of 5% for cultivating the vaccine for adapting cell. The primitive seed in step (3) is 2-5 generation. The base seeding in step (3) is 6-9 generations. The production seed in step (3) is less than 13 generations. The cultivating condition in step (4) is carried out with the dissolved oxygen content of 40-60%, the pH of 7-7.2, the temperature of 36-37 degrees C and the turning speed of the reactor of 50 revolutions per minute. The cell maintenance liquid in step (2) is the DMEM medium containing 1-2 wt.% of the serous or medium without serous and/or the cell maintenance liquid in step (2) is the DMEM medium containing 1-2 wt.% of the serous or medium without serous. The TPCK pancreatin is added in step (4) until the density of the TPCK pancreatin be 0.1-3 mu g/ml. The left glucose content in the virus liquid in step (5) is stable at 0.5-1.5 g/l. The virus liquid blood coagulation value of haemagglutination (HA) is greater than or equal to 29. The step (6) adopts high-speed freezing continuous flow centrifugal machine with the turning speed of more than 9000 revolutions per minute. The inactivation process of step (6) is carried out at 37 degrees C for more than 16 hours using formaldehyde solution with the density of 0.1 wt.%. The emulsifying process in step (6) comprises putting 2-3 parts by weight (pbw) of the oil phase into an emulsifying tank, adding 1-2 pbw of the water phase prepared by inactivated virus under stirring, and fully mixing and then emulsifying. The density of the TPCK pancreatin in step (2) or step (4) is 1.2 mu g/ml. Preferred Components: The cell is dog nephrocyte Mardin-Darby canine kidney (MDCK), African green monkey kidney cell (VERO) or chick embryo fibroblast DF-I. The bioreactor is a turbulence flow bioreactor. The cell cultivating carrier is polyester fiber carrier. ACTIVITY - Virucide.    MECHANISM OF ACTION - Vaccine.