TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Components: The substrate in the reaction system with final concentration of 0.1-1 mmole/l. The enzyme-containing thallus cell is present in an amount of 20 g/g of substrate. Preferred Method: The enzyme-containing thallus cell is prepared by inoculating Saccharomyces cerevisiae CGMCC No. 2266 into a fermentation culture medium at a rotating speed of a shaking table of 150-200 revolutions per minute and a temperature of 26-35 degrees C for 30 hours, centrifuging the fermentation liquor to obtain the enzyme-containing thallus cell. The method: comprises (1) culturing the Saccharomyces cerevisiae CGMCC No. 2266 inoculated in culture medium inclined surface at 26-35 degrees C for 4-6 days to obtain the bacteria body inclined surface, where the inclined plane culture medium comprises 5-15 g/l of malt extract, 2-4 g/l of yeast powder, 4 g/l of peptone, 7-12 g/l of glucose and 15-25 g/l of agar and has neutral pH value, and solvent is water, (2) selecting inclined plane strain to seed culture medium and then culturing in a shaking table at a temperature of 26-35 degrees C and a rotating speed of 150-200 revolutions per minute for 18-26 hours to obtain seed solution, where the seed culture medium comprises 26-32 g/l of glucose, 2-4 g/l of yeast powder, 3-6 g/l of ammonium sulfate, 0.2-0.4 g/l of anhydrous magnesium sulfate, 0.5-1.5 g/l of dipotassium hydrogen phosphate trihydrate and 0.6-1.5 g/l of potassium dihydrogen phosphate, and solvent is water, and adjusting the pH value to 7 of the liquid culture medium using sodium hydroxide or hydrochloric acid solution, (3) taking the seed liquid and then inoculating 10-20 vol.% in fermentation culture medium and culturing at 26-35 degrees C under rotating speed of the shaking table of 15-200 revolutions per minute for 18-30 hours to obtain enzyme-containing thallus cell and fermentation liquid, centrifuging and separating the liquid to obtain the enzyme-containing thallus cell, where the fermentation culture medium comprises 26-32 g/l of glucose, 2-4 g/l of yeast powder, 3 to 6 g/l of ammonium sulfate, 0.2-0.4 g/l of anhydrous magnesium sulfate, 0.5-1.5 g/l of dipotassium hydrogen phosphate trihydrate and 0.6-1.5 g/l of potassium dihydrogen phosphate, and adjusting the pH value of the liquid culture medium to 7 using sodium hydroxide or hydrochloric acid solution, and (4) adding (3S)-3-(tert-butoxycarbonyl)amino-1-chloro-4-phenyl-(2R)-butanol and 5-20% ethanol as the auxiliary substrate and then dissolving in 0.1-1 mmole/l of phosphate buffer solution at a pH of 5-8, adding enzyme-containing thallus cell to 20 times of (3S)-3-(tert-butoxycarbonyl)amino-1-chloro-4-phenyl-(2R)-butanol amount and then carrying out conversion reaction at 25-45 degrees C for 12-72 hours to obtain the conversion liquid; and further comprises (5) separating and purifying the conversion liquid to obtain (3S)-3-(tert-butoxycarbonyl)amino-1-chloro-4-phenyl-(2R)-butanol, and adding ethanol with the concentration of 5-20% in the reaction system. The step of separating and purifying (3S)-3-(tert-butoxy carbonyl)amino-1-chloro-4-phenyl-(2R)-butanol comprises centrifuging the conversion solution at 4000 revolutions per minute for 20 minutes after the reaction is finished, removing bacteria in the precipitate, taking supernatant fluid and then extracting by equal volume of ethyl acetate for 3 times, combining the ethyl acetate extracting solution, adding anhydrous sodium sulfate to remove water in the ethyl acetate extracting solution and then filtering, and taking the filtrate and then distilling to remove ethyl acetate to obtain the (3S)-3-(tert-butoxy carbonyl)amino-1-chloro-4-phenyl-(2R)-butanol.