NOVELTY - Extraction and separation of nardosinone involves adding 8-10 times spikenard medicines into 90-95 mass% ethanol solution; refluxing and extracting for 3 times for 2 hours each time; combining extracting liquids; concentrating and recycling ethanol to obtain extract; adding 2-4 times water into extract; fully stirring and dispersing; adding ethyl acetate in same volume of water solution; fully stirring; separating liquid; concentrating organic phase; recycling ethyl acetate to obtain extract; fully mixing extract with silica gel; drying; and grinding into powder.    USE - Extraction and separation of nardosinone.    ADVANTAGE - The method has simple and fast operation, low solvent consumption, high yield, and is economical and environment friendly. All reagents used are recyclable, thus no pollution is generated.    DETAILED DESCRIPTION - Extraction and separation of nardosinone comprises adding 8-10 times spikenard medicines into 90-95 mass% ethanol solution according to mass-volume ratio (kg/l); refluxing and extracting for 3 times for 2 hours each time; combining extracting liquids; concentrating and recycling ethanol to obtain extract; adding 2-4 times water into extract according to mass-volume ratio (kg/l); fully stirring and dispersing; adding ethyl acetate in same volume of water solution; fully stirring; separating liquid; concentrating organic phase; recycling ethyl acetate to obtain extract; fully mixing extract with 2 times of 60-80-meshes chromatographic silica gel; drying; grinding to powder to obtain mixed sample silica gel; weighing 200-300-mesh chromatography silica gel to silica gel column; sampling according to weight ratio of sampling amount to column packed silica gel of 1:5; eluting with petroleum ether-ethyl acetate solution according to volumetric ratio of 8:1 while monitoring with thin liquid chromatography method; absorbing chromatography eluent and nardosinone comparison product; sampling on same silica gel GF254 thin plate; spreading with petroleum ether-ethyl acetate according volumetric ratio of 4:1 as spreading agent; observing in UV lamp of 254 nm; collecting eluent in product segment; concentrating until solid starts to crystallized; standing; cooling and crystallizing for 8-10 hours; re-crystallizing according to specific purity of crystal until purity is greater than or equal to 98.5%; filtering and drying solid at 80 degrees C for 6 hours to obtain nardosinone product; heating and dissolving crude nardosinone product with petroleum ether-ethyl acetate solution according to volumetric ratio of 4:1; concentrating until solid starts to crystallized; standing; cooling and crystallizing for 8-10 hours; repeatedly crystallizing according to specific purity of crystal until purity is greater than or equal to 98.5%; and filtering and drying solid at 80 degrees C for 6 hours to obtain nardosinone product.