NOVELTY - Method for preparing bacterial cellulose using bagasse, involves drying and pulverizing the bagasse, immersing pulverized bagasse in acid, reacting, filtering and collecting hydrolyzate, adding ion exchange resin, centrifuging or filtering mixture, adding 20% peroxidase, adding 50 mM hydrogen peroxide, reacting mixture, adding alkaline solution, obtaining hydrolyzed product, adding nitrogen source, inoculating Acetobacter to the obtained medium, culturing and obtaining bacterial cellulose from the medium.    USE - Method for preparing bacterial cellulose using bagasse (claimed).    ADVANTAGE - The culture medium carbon source prepared by the method has excellent quality and low price, and is suitable for industrialized production.    DETAILED DESCRIPTION - Method for preparing bacterial cellulose using bagasse, involves (a) drying the bagasse, pulverizing the dried bagasse into powder, immersing the pulverized bagasse in 0.5-10 weight/volume% acid in the ratio of 1:5-1:20, and reacting the obtained mixture at 100-240 degrees C for 50-90 minutes, (b) filtering and collecting hydrolyzate, adding ion exchange resin (pH 10), reacting the obtained mixture at room temperature or 50 degrees C for 30-60 minutes, and centrifuging or filtering the obtained mixture to remove resin to obtain solution (pH 4-6), (c) adding ion exchange resin (pH 5.5), reacting the obtained mixture at room temperature or 50 degrees C for 30-60 minutes, and centrifuging or filtering the obtained mixture to remove resin to obtain solution (pH 4-6), (d) adding activated carbon to the obtained solution, reacting for 5-60 minutes, and centrifuging or filtering to remove the precipitate, (e) (i) adding 20% peroxidase 5 (U/ml) to the obtained product, adding 50 mM hydrogen peroxide for every 1 hour, reacting the obtained mixture at 30 degrees C in a water bath for 12-24 hours, removing hydrogen peroxide, and maintaining pH value of the obtained product at 4-6, or (ii) adding alkaline solution (pH 9-11) to the obtained product, reacting the obtained mixture at 20-60 degrees C in a water bath for 12-24 hours, and centrifuging or filtering to remove the precipitate, and (f) obtaining the hydrolyzed product, adding 0.1-2% nitrogen source, reacting the obtained mixture at 121 degrees C for 15-20 minutes to obtain a medium, inoculating 5-10% Acetobacter to the medium, shaking and culturing at 25-30 degrees C and at 160-250 revolutions per minute (rpm) for 6-25 days, and obtaining bacterial cellulose from the medium.