NOVELTY - A Metarhizium anisopliae mutant strain 11490 deposited as CCTCC M 2011240, is new.    USE - The M.anisopliae mutant strain is useful for 11 alpha hydroxylation reaction of steroids chosen from 19-norandrost-13-ethyl-androst-4-en-3,17-dione, androst-4-ene-3,17-dione and androst-1,4-diene-3,17-dione (all claimed).    ADVANTAGE - The strain effectively introduces 11 alpha hydroxyl group into steroids, has wide steroid substrate selectivity, high transformation rate and excellent application prospect, and can be cultured simply. The method is environmentally-friendly and easy to operate, and provides key intermediate for synthesis of steroid medicines.    DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for hydroxylation of steroids, which involves (a) selecting the above-mentioned strain, (b) inoculating the strain to potato dextrose agar (PDA), adding 0.25 %mass silkworm chrysalis powder, culturing at 26-30 degrees C for 5-10 days, (c) inoculating the cultured strain under sterile conditions to 40 ml liquid medium in 250 ml shake flasks and culturing at 26-30 degrees C in a shaker at 220 revolutions/minute (rpm) for 24-60 hours to obtain seed liquid, where the medium comprises 1-3 %mass glucose, 1-3 %mass soybean meal and 0.25-1 %mass silkworm chrysalis powder, and has pH of 6-7, (d) inoculating 5-15 vol.% seed liquid to liquid culture medium and culturing at 26-30 degrees C in rotary shaker at 220 rpm for 24-60 hours to obtain fermentation broth, (e) allowing the substrate to be transformed, previously dissolved in 1 %mass Tween 80 (RTM: Polysorbate 80) to stand, sonicating the solution for 30 minutes, adding 1-3 %mass solution into the fermentation broth and fermenting at 26-30 degrees C in rotary shaker at 220 rpm for 48-96 hours to obtain the fermented broth, and (f) centrifuging the fermentation broth, removing cells after filtration, washing the filtrate with 1/2 volume of ethyl acetate, extracting the filtrate 3 times, combining the extracts, decompressing and concentrating the combined extract under reduced pressure, adding saturated salt solution, drying by anhydrous sodium sulfate, subjecting to silica gel column chromatography, and immersing the recovered cells in ethyl acetate.