NOVELTY - Preparing oleanolic acid and echinocystic acid from Gledisia Sinensis Lam involves extracting Gledisia Sinensis Lam: weighing 100 g Gledisia Sinensis Lam, using 75% of ethanol to reflux and extract for 2 hours, filtering extract, combining decoction for 3 times extraction liquid, recycling ethanol, condensing, decompressing and drying, crushing to obtain Gledisia Sinensis Lam, taking 10 g Gledisia Sinensis Lam, adding 3-5 g diatomite, stirring uniformly, in Soxhlet extractor, adding methanol, refluxing, cooling, evaporating to dryness and crystallizing.    USE - For preparing oleanolic acid and echinocystic acid from Gledisia Sinensis Lam (claimed).    ADVANTAGE - The method does not need expensive device, is simple and easy, is low cost, and it can reach the quality requirement of traditional Chinese medicine chemical reference substance.    DETAILED DESCRIPTION - Preparing oleanolic acid and echinocystic acid from Gledisia Sinensis Lam involves extracting Gledisia Sinensis Lam: weighing 100 g Gledisia Sinensis Lam, using 75% of ethanol to reflux and extract for 2 hours, filtering, residue and then using ethanol to extract filtering, combining the decoction for 3 times extraction liquid, recycling the ethanol, condensing it into extract, at 60-65 degrees C, decompressing and drying, crushing to obtain Gledisia Sinensis Lam, (2) taking 10 g Gledisia Sinensis Lam, adding 3-5 g diatomite, stirring uniformly, in the Soxhlet extractor, adding methanol, refluxing for 3 hours, cooling, evaporating to dryness, leavings adding diluted hydrochloric acid to dissolve, transferring to a round bottom flask, heating in water bath to hydrolyze for 2 hours, cooling, in the separating funnel, extracting for 3 times by chloroform, combining the chloroform extracting solution, using water to wash for 2 times, discarding the water solution, recycling chloroform, evaporating to dryness, leavings with methanol, 4 degrees C, separating out white crystal, filtering the crystal, the mother liquor 10 ml is concentrated to, above, and crystallizing and separating out and filtering the crystal, combining the crystal, using methanol to wash crystal, at 55-60 degrees C and drying to obtain the dry and crystallizing, (3) oleanolic acid and thin-layer puncture of echinocystic acid is prepared as follows: thin layer for preparing the test sample solution: taking the dry crystal, using methanol as a solution with the concentration of 26 mg/ml as a thin layer for a test sample, a sample and absorbing the sample solution by a micro sampler, a strip-shaped or point-shaped continuous form with thin-layer plate, containing a chromatography cylinder developing agent, taking out, air-curing, two sides of the thin layer, chromatography plate taking oleanolic of echinocystic acid and color spot corresponding to a non-color region respectively scraping the oleanolic acid and the scraped of echinocystic acid silica gel powder are placed in a conical flask, adding methanol, performing ultrasonic treatment for 30 minutes, centrifuging, filtering the clear liquid, then adding methanol, ultrasonic, centrifuging repeatedly for 2 times, combining the three ultrasonic filtrate, recycling methanol, evaporating to dryness, namely obtaining oleanolic acid preparation is capsule and acid.