TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Method: The primary fraction comprises rind. The secondary fraction comprises pith, leaves, or sugars. The lignocellulosic feedstock is separated into at least ( a)primary fraction, secondary fraction, and third fraction, (b) primary fraction, secondary fraction, third fraction, and fourth fraction, or (c) primary fraction, secondary fraction, third fraction, fourth fraction, and fifth fraction. The method (M1) involves using automated separation device to separate lignocellulosic feedstock, extracting juice from lignocellulosic feedstock and subsequently separating lignocellulosic feedstock, separating the lignocellulosic feedstock and subsequently extracting juice from the lignocellulosic feedstock, or pretreating at least one of the fractions before to any substantial extraction of juice. The method (M1) further involves generating primary fraction enriched in water and a secondary fraction depleted in water, where the fraction depleted in water is combusted, generating a primary fraction enriched in cellulose, generating a primary fraction enriched in hemicellulose, generating a primary fraction enriched in lignin, pretreating each of fractions separately or together, burning at least one of the fractions to generate steam and electricity, converting at least one of the fractions to one or more sugars, and converting sugars into renewable material. The fraction is pretreated using a harsh pretreatment process, or pith and/or leaves using mild pretreatment process. The juice has not been substantially separated. The method (M1) involves using cane tissue fractionation system at a front end of an existing sugar mill to separate the lignocellulosic feedstock into the fractions, and further involves sending the juice fraction to a sucrose production train which is portion of existing sugar mill. The method (M1) further involves sending the rind fraction to cane handling equipment for sugar recovery, sending a bagasse remainder to a boiler for power production, sending the sugar either to sucrose recovery unit or to fermentation unit, pretreating the pith fraction by hydrolyzing the pith fraction with low or no acid for at least 110 degrees C, further hydrolyzing the pith fraction with an enzyme cocktail to produce sugars for fermentation, sending wax from the wax cut fraction to a wax recovery unit, burning remaining fiber from the wax cut, pretreating the trash fraction by hydrolyzing the trash fraction with low or no acid at a temperature of at least 110 degrees C, and further hydrolyzing the trash fraction with an enzyme cocktail to produce sugars for fermentation. Preferred Components: The lignocellulosic feedstock comprises at least one compound chosen from cellulose, hemicellulose, lignin, and their combinations. The pretreatment involves using one or more ionic liquid, acid, base, enzyme, and water. The acid comprises at least one inorganic acid, organic acid, mineral acid, bronsted acid, Lewis acid, and their combinations and is chosen from sulfuric acid, hydrochloric acid, sulfonic acid, phosphoric acid, nitric acid, acetic acid, lactic acid, formic acid, oxalic acid, and their combinations. The base comprises at least one inorganic base, organic base, mineral base, bronsted base, Lewis base, and their combinations and is chosen from ammonia, ammonium hydroxide, sodium hydroxide, potassium hydroxide, magnesium hydroxide, lime, calcium hydroxide, calcium oxide, and their combinations. The enzyme is chosen from cellulase, glucanase, endoglucanase, exoglucanase, cellobiohydrolase, glucosidase, hemicellulase, esterase, acetyl xylan esterase, pectinase, and their combinations. The pretreatment is performed without heating at least one fraction. The sugar is chosen from sucrose, glucose, fructose, mannose, galactose, xylose, arabinose, hexose, pentose, cellobiose, oligosaccharides, and their combinations. The renewable material comprises at least one compound chosen from ethanol, n-butanol, isobutanol, 2-butanol, fatty alcohols, isobutene, isoprenoids, triglycerides, lipids, fatty acids, lactic acid, acetic acid, propanediol, butanediol, and their combinations, or one or more biofuel components, alcohol, ethanol, butanol, isobutanol or lipids, and suitable for use as biofuels, blendstocks, chemicals, intermediates, solvents, adhesives, polymers, and/or lubricants. The lignocellulosic feedstock comprises at least one energycane, sugarcane, miscanthus, napier grass, sorghum, arundo, switchgrass, and their hybrids, or grasses, legumes, forbs, hardwoods, softwoods, municipal solid waste, paper mill residue, forest litter, fruit waste, citrus waste, and agricultural residues. The primary fraction comprises 30-50 wt.% (dry weight) cellulose (dry weight), 15-30 wt.% hemicellulose or 15-25 wt.% lignin (dry weight). The secondary fraction comprises 15-40 wt.% (dry weight) cellulose, 9-25 wt.% (dry weight) hemicellulose, or 5-19 wt.% (dry weight) lignin. The biofuel comprises alcohol. The biofuel component comprises ethanol, preferably butanol. Preferred Unit: The biorefmery comprises cane tissue fractionation system at front end of a sugar mill and separates tissues of lignocellulosic feedstock into juice, rind, pith, wax cut, and trash. The cane handling equipment is portion of an existing sugar mill. EXAMPLE - No suitable example given.