TECHNOLOGY FOCUS - BIOLOGY - Preferred Method: The method comprises placing Bacillus pumilus in a culture medium comprising 5 g of peptone, 3 g of beef extract, 5 g of sodium chloride, 15 g of agar and 1000 l of distilled water, adjusting the pH value to 7 and then cultivating, adding water and 5 mg of manganese sulfate monohydrate, placing Streptococcus lactis in a culture medium comprising 10 g of casein peptone, 10 g of beef extract, 5 g of yeast powder, 5 g of glucose, 5 g of sodium acetate, 5 g of citric acid, 1 g of Tween-80 (RTM: Polysorbate 80), 2 g of dipotassium hydrogen phosphate, 2 g of magnesium sulfate heptahydrate, 0.05 g of manganese sulfate monohydrate, 20 g of calcium carbonate, 15 g of agar and 1 l of distilled water, and adjusting the pH value to 6-8, placing Pseudomonas sp. in lysogeny broth liquid culture medium, inoculating strain 200 and 10 l of PR culture medium, performing culture medium in a fermentation temperature to 28 degrees C, adjusting pH value of 6-8, adding ammonium hydroxide solution (28%), stirring the mixture at a speed of 400 (rpm) and then ventilating amount of 0.4 (volume/volume), when the concentration of the biological quantity reaches 10-40 g/l, stopping the fermentation and then cultivating for 24 hours, placing Pantoea agglomerans in a R4F culture medium, inoculating strain 250, and 10 l of R4F culture medium, performing culture medium in a fermentation temperature to 28 degrees C, adding ammonium hydroxide solution (28%), adjusting the pH value to 6-8, preferably 7, stirring the mixture at a speed of 100 rpm and then ventilating amount of 0.2 (v/v), when the concentration of the biological quantity reaches 10-30 g/l, and stopping fermentation. Preferred Components: The R4F culture medium comprises 1000 ml of distilled water, 2 g of beef extract, 3 g of peptone, 0.05 g of magnesium sulfate heptahydrate, 0.5 g of dipotassium hydrogen phosphate, 0.05 g of ferrous sulfate heptahydrate, 0.05 g of manganese sulfate monohydrate, 20 g of cane sugar and 18-20 g of agar phospholipid powder.