NOVELTY - Preparation of multifunctional biological protein controlled release fertilizer comprises crushing rapeseed cake, feather meal, bone powder and cottonseed or straw additive; fermenting bacterial strains into fermentation culture medium, flash evaporating and drying; mixing germ powder, dry fungi powder, carboxymethyl cellulose and water, adding coating agent to granular organic fertilizer surface, and drying; spraying nitrification inhibitor oil solution to urea surface; and mixing uniformly nitrification inhibitor coated urea, monoammonium phosphate, potassium chloride or potassium sulfate.    USE - Method for preparing multifunctional biological protein controlled release fertilizer (claimed).    ADVANTAGE - The fertilizer restores activity of soil, and improves soil fertility, disease resistance and yield.    DETAILED DESCRIPTION - Preparation of multifunctional biological protein controlled release fertilizer comprises: (A) taking rapeseed cake, feather meal, bone powder and additive at weight ratio of 25-35:25-35:18-22:18-22, mixing raw material, crushing into granular organic fertilizer, where additive is cottonseed or straw; (B) taking bacterial strains into the fermentation culture medium, fermenting, flash evaporating and drying ferment bacteria liquid to obtain rejuvenation agent, where bacterial strains are Bacillus licheniformis (CCTCC M2013021), Bacillus subtilis (CCTCC M2013019) and Bacillus amyloliquefaciens (CCTCC M2013020); (C) mixing germ powder, dry fungi powder, carboxymethyl cellulose and water at mass ratio of 2.25-5:0.03-0.05:1.5-3 to obtain coating agent, where coating agent and granular organic fertilizer weight ratio is 3.78-8.05:470-1000, coating the coating agent to granular organic fertilizer surface, and drying at 40-50 degrees C; (D) taking nitrification inhibitor and no. 68 machine oil at weight ratio of 0.8-1:2.6-3, pouring nitrification inhibitor to no. 68 mechanical oil, heating to 68-72.5 degrees C, stirring, taking nitrification inhibitor oil solution and urea at weight ratio of 0.98-1:980:1000, spraying nitrification inhibitor oil solution to surface of urea, where nitrification inhibitor is 2-chloro-6-trimethylpyridine (sic); and (E) taking 20000000/g biological bacteria, nitrogen element, phosphorus element, potassium element and organic matter at mass ratio of 13-28:4-20:5-17:15-20, and mixing uniformly nitrification inhibitor coated urea, monoammonium phosphate, potassium chloride or potassium sulfate.