NOVELTY - Preparing acridine series chemiluminescent agent comprises e.g. dissolving 4-aminobutyric acid in first solvent, stirring, cooling, adding triethylamine and p-methyl benzene sulfonyl chloride, reacting, regulating pH, extracting, vacuum extracting to obtain p-toluenesulfonylaminobutyric acid, placing 9-acridine carboxylic acid in a flask, adding solvent, reacting, filtering, dissolving p-toluenesulfonylaminobutyric acid in solvent, adding inorganic alkali, reacting, adding filtrate A, using water to quench reaction, vacuum extracting, eluting to obtain product of (III).    USE - The method is useful for preparing acridine series chemiluminescent agent (claimed).    ADVANTAGE - The method has simple preparation techniques, is environmentally friendly, and economical.    DETAILED DESCRIPTION - Preparing acridine series chemiluminescent agent comprises (i) dissolving 4-aminobutyric acid in first solvent, stirring, cooling, adding triethylamine and p-methyl benzene sulfonyl chloride, maintaining the temperature, after finishing reaction, regulating pH value, extracting product, drying the organic phase, vacuum extracting to remove the solvent to obtain product p-toluenesulfonylaminobutyric acid, (ii) placing 9-acridine carboxylic acid in a flask, adding the second solvent and dissolving, stirring, cooling, adding the third solvent, reacting, filtering solid and collecting the filtrate to obtain the filtrate, dissolving p-toluenesulfonylaminobutyric acid in a solvent, cooling, slowly adding inorganic alkali, reacting, slowly adding the filtrate A to the reaction system, placing the reaction system at room temperature and reacting overnight, using water to quench reaction, vacuum extracting to remove solvent, adjusting using hydrochloric acid, extracting the product, and drying the organic phase, vacuum extracting to remove the solvent to obtain crude product, purifying the crude product by column chromatography, eluting using eluent to obtain the product, i.e. acridin-9-carbonyl-(p-toluenesulfonyl)aminobutyric acid of formula (III), (iii) placing (III) in a flask, adding 1,3-propane sultone, stirring, heating reaction and cooling to room temperature, pumping, washing, drying, filtering and collecting solid to obtain 9-((3-carboxypropyl)-(p-toluenesulfonyl)carbamyl)-10-(3-sulfonatopropyl)acridin-10-ium of formula (IV) crude product, and (iv) dissolving (IV) in N,N-dimethyl formamide (solvent four), stirring and in turn adding triethylamine and 3-succinimido-1,1,3,3-tetramethyluronium tetrafluoroborate, separating and purifying the reaction product by liquid-phase chromatogram to obtain the product 9-((3-succinimidyloxycarbonylpropyl)-(p-toluenesulfonyl)carbamyl)-10-(3-sulfonatopropyl)acridin-10-ium of formula (V).    R = H or OCH3.