NOVELTY - Deacetyl asperulosidic acid methyl ester and scandoside methyl ester are extracted by adding Gardenia jasminoides material with ethanol to reflux and extract to obtain concentrated liquid; eluting concentrated liquid with alcohol; sending alcohol elution liquid to a silica gel column, then eluting with chloroform-methanol solution, collecting elution liquid, removing solvent, and collecting residue; and dissolving residue with water and performing low-pressure liquid phase separation using methanol or acetonitrile solution as mobile phase.    USE - Method for extracting deacetyl asperulosidic acid methyl ester and scandoside methyl ester (claimed).    ADVANTAGE - The method is simple and easy to operate, does not pollute the environment by utilizing limited amount of harmful solvent, has high yield, and can obtain high purity product and can separate two monomer components at one time. Further, the column material can be repeatedly used.    DETAILED DESCRIPTION - Extraction of deacetyl asperulosidic acid methyl ester and scandoside methyl ester comprises: (A) adding Gardenia jasminoides material with ethanol to reflux and extract, condensing alcohol extract solution, adding ethanol to precipitate for 16-24 hours, filtering, recycling alcohol, and concentrating to obtain concentrated liquid; (B) sending condensed liquid to a macroporous resin column, eluting with purified water, detecting and monitoring by HPLC, collecting target eluent, decompressing and concentrating to obtain concentrated elution liquid, adjusting pH value of concentrated liquid to 1-3, and heating in water bath for 30 minutes; (C) eluting water bath heated concentrated elution liquid with purified water, then eluting with 30 vol.% alcohol, collecting alcohol elution liquid, decompressing and concentrating into 5 g medicine material/ml; (D) sending concentrated liquid to a silica gel column, then eluting with chloroform-methanol solution (10:1), collecting elution liquid, recycling solvent, and collecting residue; and (E) dissolving residue with water, performing low-pressure liquid phase separation using methanol or acetonitrile solution as mobile phase, collecting deacetyl asperulosidic acid methyl ester and scandoside methyl ester elution liquids, removing solvent, dissolving residue with water, and freeze-drying to obtain deacetyl asperulosidic acid methyl ester and scandoside methyl ester.