NOVELTY - A monotropein monomer separating method involves crushing dried Pyrola herb into Pyrola segment. The Pyrola segment is extracted with a polar solvent by reflux extraction to obtain an extract, where the unit of the Pyrola segment and polar solvent is kilograms and liters, respectively. The extract is concentrated to obtain concentrated solution, which is subjected to enrichment by feeding AB-8 macroporous resin for secondary adsorption to obtain enriched product. The enriched product is washed with ethanol solution and water.    USE - Method for separating monotropein from Pyrola.    ADVANTAGE - The method enables separating monotropein with high yield in a simple, efficient, inexpensive and eco-friendly manner, and realizing high industrial production effect.    DETAILED DESCRIPTION - A monotropein monomer separating method involves crushing dried Pyrola herb into Pyrola segment with length of 2-5 mm. The Pyrola segment is extracted with a polar solvent at ratio of 1:10-30 by reflux extraction at 40-80 degrees C for 5-10 hours to obtain an extract, where the unit of the Pyrola segment and polar solvent is kilograms and liters, respectively. The extract is concentrated to obtain concentrated solution, which is subjected to enrichment by feeding AB-8 macroporous resin for secondary adsorption to obtain enriched product. The enriched product is washed with 20-40% ethanol solution and 80-95% water. The 20-40% ethanol solution is collected and concentrated to ethane, followed by absorbing the enriched product by 70-90% ethanol solution, followed by collecting analytic solution at 40-60 degrees C by extracting the analytic solution by using 60-80 meshes silica gel. The analytic solution is dried to obtain dispersed granular raw material, followed by adding 200-300 meshes of silica gel with ethyl acetate-methanol solution at a volume ratio of 25:1-15:1 in wet method column to separate dispersed granular raw material by performing gradient elution with ethyl acetate-methanol solution with a volume ratio of 25:1-15:1 in separating column with diameter of 10-30 cm, where the dispersed granular raw material comprises raw material and silica gel at a mass ratio of 1:(2-4). The resultant product is modified by using ethyl acetate-methanol solution at a volume ratio of 20:1-5:1 and trimeric sodium phosphate solid at a mass ratio of 1:0.5-2, followed by collecting eluent-A as target product solution to obtain monotropein coarse solution. The obtained solution is decompressed at 40-60 degrees C, followed by recycling reagent to obtain crystal of saponin crude product, which is dried and dissolved in water to obtain dissolved product. The dissolved product is transferred in an activated material separating column and eluted with water, water washing liquid and 60-80% ethanol solution to obtain eluent-B. The eluent-B is decompressed to obtain filtrate. The filtrate is concentrated and dried by vacuum and normal pressure drying or freeze drying process to obtain a monotropein monomer.