NOVELTY - A transgenic Pseudomonas aeruginosa stably transformed with an isolated nucleic acid molecule, is new. The microbial cell exhibits decreased expression of the endogenous nucleotide sequence of interest and the cell exhibits an altered electrogenic efficacy. The P.aeruginosa comprises at least three endogenous nucleotide sequences of interest that are disrupted.    USE - The transgenic P.aeruginosa is used in cleaning solutions and odor reduction systems.    ADVANTAGE - The transgenic P.aeruginosa has improved function in microbial fuel cells, improved power (electron) generation, improved electron transfer, improved contact with the anode, improved biofilm formation, reduced proliferative capability, reduced virulence in mammals or plants, altered iron-containing cytochrome concentration, preferably iron-containing cytochrome concentration at or near the cytoplasmic membrane of the cell, and altered concentration of channel forming protein, preferably the channel forming concentration of the outer membrane of the cell, and exhibits increased current output/bacterial cell when the bacterial cell is a component of a microbial fuel cell, increased electron transfer to an anode directly or indirectly, reduced motility, altered pilus sticking, and altered twitching motility as compared with nontransgenic cell.    DETAILED DESCRIPTION - A transgenic Pseudomonas aeruginosa stably transformed with an isolated nucleic acid molecule, is new. The isolated nucleic acid molecule disrupts an endogenous nucleotide sequence of interest chosen from (a) pilT comprising a 1035 base pair sequence (SEQ ID NO: 1) fully defined in the specification, (b) type IV pilus genes (pilA) comprising a 450 base pair sequence (SEQ ID NO: 2) fully defined in the specification, (c) nitrite reductase gene (nirS) comprising a 1707 base pair sequence (SEQ ID NO: 3)fully defined in the specification, (d) bdlA comprising a 1254 base pair sequence (SEQ ID NO: 4) fully defined in the specification, (e) lasI comprising a 606 base pair sequence (SEQ ID NO: 5) fully defined in the specification, (f) lasR comprising a 720 base pair (SEQ ID NO: 6) fully defined in the specification, (g) filamenting temperature sensitive mutant Z, ref.1 (ftsZ) comprising a 1185 base pair sequence (SEQ ID NO: 7) fully defined in the specification, and (h) fliC comprising a 1467 base pair sequence (SEQ ID NO: 8) fully defined in the specification. The microbial cell exhibits decreased expression of the endogenous nucleotide sequence of interest and the cell exhibits an altered electrogenic efficacy. The P.aeruginosa comprises at least three endogenous nucleotide sequences of interest that are disrupted. INDEPENDENT CLAIMS are included for the following: (1) biofilm comprising a transgenic bacterial cell where the bacterial cell is the above-mentioned transgenic P.aeruginosa cell; (2) filtration device comprising the biofilm; (3) microbial fuel cell comprising the biofilm, an anode chamber and a cathode chamber; (4) sensor for detecting a predetermined compound, comprising the microbial fuel cell and an indicator, where the indicator indicates an abnormal level of the compound; and (5) bioremedial system comprising the microbial fuel cell, where the transgenic bacterial cell utilizes a predetermined compound as a feedstock and the cell converts the predetermined compound into a second compound.