NOVELTY - Preparing total flavone, total alkaloid and total polysaccharide from mulberry bark, involves smashing mulberry bark, adding ethanol to the smashed product, heat preserving in a slight boiling state, performing extraction, combining the extracting solution, reducing pressure of the extracting solution, recovering ethanol, concentrating the solution, filtering the concentrated solution, passing the filtrate through 2 sections of high-voltage resin in series, eluting 2 sections of the resin, eluting macroporous resin column, and eluting ion exchange column.    USE - The method is useful for preparing total flavone, total alkaloid and total polysaccharide from mulberry bark (claimed).    ADVANTAGE - The method is environmentally-friendly, simple, rapid, cost-effective and innoxious. The total flavone, total alkaloid and total polysaccharide prepared by the method from mulberry bark has purity of 60% or more, 40% or more and 50% or more respectively.    DETAILED DESCRIPTION - Method for preparing total flavone, total alkaloid and total polysaccharide from mulberry bark, involves smashing mulberry bark using 10-40 mesh size sieves, adding 8 times 60% ethanol to the smashed product, heat preserving for 1 hour in a slight boiling state, subsequently adding 6 times, 60% ethanol to the smashed product, heat preserving for 1 hour in a slight boiling state, performing extraction for 2 times, combining the extracting solution, reducing pressure of the extracting solution, recovering ethanol (0.1-0.2 g crude drug/ml), concentrating the solution, filtering the concentrated solution using 300-400 mesh filtering bag, passing the filtrate through 2 sections of high-voltage resin in series (PIPO-01 macroporous resin and 732 strong acid type cation exchange resin), eluting 2 sections of the resin using 10 BV water, eluting macroporous resin column using 6 BV and 80% ethanol, and eluting ion exchange column using 0.5 mol/l ammonia water, where ethanol, ammonia water and unabsorbed liquid passing through 2 sections of high-voltage resin in series are conducted to pressure reduction, concentrated solution is obtained after recovery, and concentrated solution is freeze-dried.