TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Method: The preparation of acridine series chemiluminescent agent comprises (i) dissolving 4-aminobutyric acid in first solvent, stirring, cooling, adding triethylamine and p-methyl benzene sulfonyl chloride, maintaining the temperature, after finishing reaction, regulating pH value using hydrochloric acid to 3, extracting the product twice using ethyl acetate, adding anhydrous sodium sulfate into the organic phase, vacuum extracting to remove the solvent to obtain the product, i.e. p-toluenesulfonylaminobutyric acid, (ii) adding 1-methoxy-9-acridine formic acid into the flask, adding the second solvent and dissolving, stirring, cooling, adding triethylamine and ethyl chloroformate and reacting, filtering the solid and collecting the filtrate to obtain the filtrate A, placing p-toluenesulfonylaminobutyric acid in flask, adding second solvent to dissolve it, cooling, slowly adding sodium hydroxide, slowly adding the filtrate A to the reaction system, raising the reaction system temperature to room temperature and reacting, using water to quench the reaction, vacuum extracting to remove the solvent, adjusting pH, extracting the product, adding anhydrous sodium sulfate in organic phase, vacuum extracting to remove the solvent to obtain crude product, purifying the crude product by column chromatography, eluting with eluent to obtain the product that is acridin-9-carbonyl-(p-toluenesulfonyl)aminobutyric acid (III), (iii) placing (III) in a flask, adding 1,3-propane sultone, magnetic force stirring, heating and reacting, cooling to room temperature, adding ethyl acetate, washing, filtering and collecting the solid to obtain 9-((3-carboxypropyl)-(p-toluenesulfonyl)carbamyl)-10-(3-sulfonatopropyl)acridin-10-ium (IV) crude product, and (iv) dissolving (IV) crude product in the solvent, magnetic force stirring at room temperature and adding triethylamine and 3-succinimido-1,1,3,3-tetramethyluronium tetrafluoroborate, reacting, separating and purifying the reaction product by liquid-phase chromatogram to obtain the product 9-((3-succinimidyloxycarbonylpropyl)-(p-toluenesulfonyl)carbamyl)-10-(3-sulfonatopropyl)acridin-10-ium (V). In the step (i), the cooling temperature is 0 degrees C. In the step (ii), the cooling temperature is -10 degrees C. In the step (i) and (ii), the pH is adjusted to 3. The volume ratio of eluent ethyl acetate and petroleum ether in mixed solvent is 1:1. In the step (iii), heating to 125 degrees C, the reaction time is 1 hour. Preferred Components: The first solvent is dichloromethane, the second solvent is tetrahydrofuran, the third solvent is triethylamine and ethyl chloroformate. The extracting agent is ethyl acetate. The fourth solvent is N,N-dimethyl formamide.