NOVELTY - Improving natamycin yield by using mortierella alpina ferment waste liquid, involves 1) shaking seed culture under aseptic condition, taking Streptomyces natalensis spore coated culture based on agar inclined plane, culturing medium to culture, then putting the culture temperature culture scraper into the triangular flask with a rocker bottle seed culture medium, shaking culture the spore is 26-30 degrees C, and the culturing time is 6-9d, the shake culture is 150-200 rpm, the culture temperature is 26-30 degrees C, culture time is 36-48 hours, 2) fermentation culturing the seed liquid to 5-10% inoculum concentration of insert containing fermentation culture medium in triangular flask, the culture temperature is 26-30 degrees C, speed of the shaking table is 150-200 revolution per minute (rpm), and the fermentation time is 96-120 hours, at the same time, the fermentation time to buffer solution 7-7.2, after fermentation to obtain the natamycin, the fermentation culture medium is glucose 5-15 g/L, soluble starch (30), green pigment (50 g/L), yeast extract (5-10 g/L), yellow bean cake powder (20-40 g/L), MQ organic silicon resin, Mortierella is curvularia ferment waste liquid 10-70 vol./vol.%, and buffer solution (pH 7).    USE - For improving natamycin yield (claimed).    ADVANTAGE - The method has simple operation, short production period, is low cost and high yield, energy saving and environment-friendly.    DETAILED DESCRIPTION - Improving natamycin yield by using mortierella alpine ferment waste liquid, involves 1) shaking seed culture under aseptic condition, taking Streptomyces natalensis spore coated culture based on agar inclined plane, culturing medium to culture, then putting the culture temperature culture scraper into the triangular flask with a rocker bottle seed culture medium, shaking culture the spore is 26-30 degrees C, and the culturing time is 6-9d, the shake culture is 150-200 revolution per minute (rpm), the culture temperature is 26-30 degrees C, culture time is 36-48 hours, 2) fermentation culturing the seed liquid to 5-10% inoculum concentration of insert containing fermentation culture medium in triangular flask, the culture temperature is 26-30 degrees C, speed of the shaking table is 150-200 rpm, and the fermentation time is 96-120 hours, at the same time, the fermentation time to buffer solution 7-7.2, after fermentation to obtain the natamycin, the fermentation culture medium is glucose 5-15 g/L, soluble starch (30), green pigment (50 g/L), yeast extract (5-10 g/L), yellow bean cake powder (20-40 g/L), MQ organic silicon resin, Mortierella is curvularia ferment waste liquid 10-70 vol./vol.%, and buffer solution (pH 7).