NOVELTY - Separation and purification of iodixanol injection raw materials involves dissolving and synthesizing iodixanol raw materials with water, carrying out adsorption separation and purification using PIPO-02 macroporous resin column, using non-linear chromatography for carrying out pressing continuous chromatography, carrying out segmentation elution with ethanol, and activating and recycling separation materials.    USE - Separation and purification of iodixanol injection raw material by macroporous resin (claimed).    ADVANTAGE - The method is simple, economical, efficient and environmentally-friendly. PIPO-02 macroporous resin has excellent advantages over C-18 separating material. The cost of PIPO-02 macroporous resin is only 20% of that of C-18 reverse phase silica gel. The adsorption capacity of PIPO-02 macroporous resin is 2.5 times that of C-18 separating material. The non-linear chromatography method provides 98% iodixanol products for injection to the maximum limit.    DETAILED DESCRIPTION - Iodixanol injection raw materials are ultrasonically dissolved in 10-times water, to obtain an aqueous solution. Remaining water is added for complete dissolution. The solution is loaded in a PIPO-02 column until the solution overflows at the end. The solution is subjected to multi-segmented pressure column elution by flowing into a second PIPO-02 column. The impurities are eluted with 5% ethanol. The second column is connected to a third column, and iodixanol is washed with 15-25% ethanol. All the columns are eluted with 95% ethanol, concentrated, and dried to obtain iodixanol injection products with a purity of greater than 98%. After high-performance liquid chromatography, the purity of iodixanol product is confirmed to be greater than 98%. Column online activation regeneration is then carried out by pumping an acid into the column, and washing with 5% aqueous sodium hydroxide, 5% hydrochloric acid aqueous solution, and water in an amount 3-5 times the column volume.