NOVELTY - Microwave-assisted aqueous two-phase/reversed micelles extraction method of Sophora flavescens alkaloids, involves mixing radix sophorae flavescentis powder with ethanol-ammonium sulfate aqueous two-phase agent, obtaining radix sophorae flavescentis alkaloid aqueous solution, mixing solution with sodium dodecyl benzene sulfonate-isooctane-n-octanol reversed micelle agent, obtaining coarse product of radix sophorae flavescentis alkaloids, separating product, collecting eluent, recrystallizing eluents and obtaining matrine, oxymatrine and sophoridine monomer.    USE - The method is useful for extracting S.flavescens alkaloids.    ADVANTAGE - The method combines reversed micelles extraction, microwave-assisted extraction and aqueous two-phase extraction technologies, is simple, rapid and environmentally-friendly, has low requirements for facilities, allows easy and continuous production and scale-up, extracts S.flavescens alkaloids with purity above 90.92% and yield of 55.38 mg/g, and separates matrine, oxymatrine and sophoridine with high purity of more than 94% and little organic solvent residue.    DETAILED DESCRIPTION - Microwave-assisted aqueous two-phase/reversed micelles extraction method of Sophora flavescens alkaloids, involves (a) preparing ethanol-ammonium sulfate aqueous two-phase agent containing ammonium sulfate (18%) and ethanol (28%), preparing sodium dodecyl benzene sulfonate (SDBS)-isooctane-n-octanol reversed micelle agent, (b) crushing radix sophorae flavescentis or subprostrate Sophora powder, mixing the powder with the ethanol-ammonium sulfate aqueous two-phase agent, extracting the powder with microwave assistance, cooling the extracted liquid, centrifuging the liquid and spin-drying the upper phase, (c) dissolving the residue after spin-drying with acid, diluting the solution with water, adjusting the pH to 5 and obtaining radix sophorae flavescentis alkaloid aqueous solution, (d) mixing the radix sophorae flavescentis alkaloids aqueous solution with SDBS-isooctane-n-octanol reversed micelle agent, stirring the solution, extracting the solution, allowing the solution to stand, separating the phases, where the upper layer is reversed micelle layer and the lower layer is aqueous phase, mixing the reversed micelle layer with potassium chloride solution, stirring the solution, reverse extracting the solution, allowing the solution to stand, combining the solution with lower aqueous phase, performing rotary evaporation, desalinating the solution, filtering the solution, washing the solution, obtaining the filtrate for spin drying and obtaining coarse product of radix sophorae flavescentis alkaloids, and (e) using silica gel column chromatography for separating the coarse product of the radix sophorae flavescentis alkaloids, eluting the column with chloroform-methanol system, collecting the eluent, using thin-layer chromatography for combining different eluents, concentrating the eluent, recrystallizing the eluents, vacuum-drying the crystals and obtaining matrine, oxymatrine and sophoridine monomer.