NOVELTY - Icariin and icarisid II are extracted and separated from Epimedium by extracting Epimedium medicinal material with water; passing concentrated extract solution through a nonpolar or polar macroporous absorption resin column, then eluting with ethanol, collecting 40-45% (v/v) ethanol eluent as eluent I and 50-70% (v/v) ethanol eluent as eluent II; respectively recycling ethanol in eluent I and eluent, then extracting with water-ethyl acetate system, collecting ethyl acetate layer, filtering, and separating out solid to obtain icariin I and icariin II crude products.    USE - Method of extracting and separating icariin and icarisid II from Epimedium (claimed).    ADVANTAGE - The method has simplified process flow, obtains high purity intermediate product, increases yield of monomer component, improves utilization rate of medicine material, reduces production cost, at the same time, and does not use toxic organic solvent. Thus, the method ensures safety of operator, realizes green production, and is suitable for industrialization.    DETAILED DESCRIPTION - Extraction and separation of Icariin and icarisid II from Epimedium comprise: (A) extracting Epimedium medicinal material with water, decompressing and concentrating obtained clear liquid to obtain concentrated solution; (B) passing the concentrated solution through a nonpolar or polar macroporous absorption resin column, then sequentially eluting with 20-70% (v/v) ethanol, 40-45% (v/v) ethanol and 50-70% (v/v) ethanol, collecting 40-45% (v/v) ethanol eluent as eluent I and 50-70% (v/v) ethanol eluent as eluent II; (C)recycling ethanol in eluent I, then extracting with water-ethyl acetate system, collecting ethyl acetate layer, recycling solvent, adding 50-70% (v/v) ethanol to dissolve, filtering, refrigerating and standing, and separating out solid to obtain icariin crude product; and (D) recycling ethanol in eluent II, extracting with water-ethyl acetate system, collecting ethyl acetate layer, recycling solvent, adding 50-70% (v/v) ethanol to dissolve, filtering, refrigerating and standing, and separating out solid to obtain icarisid II crude product.