NOVELTY - Producing at least a first enzyme from a host cell, comprises cultivating the host cell in a sugar depleted cultivation environment comprising the host cell, water and a solid composition to produce the first enzyme for a cultivation time, where the first enzyme hydrolyzes a first pre-treated ligno-cellulosic biomass, and the composition: comprises a complex sugar and a lignin; is obtained from a second pre-treated ligno-cellulosic biomass; and comprises a complex sugar of the second pre-treated ligno-cellulosic biomass and a lignin of the second pre-treated ligno-cellulosic biomass.    USE - The process is useful for producing at least a first enzyme from a host cell to hydrolyze ligno-cellulosic biomass (all claimed), which is used for bio-ethanol production, where the host cell is of Penicillium decumbens, Aspergillus awamori, Fusarium culmorum, Chrysosporium lucknowense, Coprinus cinereus, Thielavia terrestris, Bacillus brevis, Bacillus circulans or Bacillus coagulans.    ADVANTAGE - The process produces the enzyme from the host cell in a cost-effective manner.    DETAILED DESCRIPTION - Producing at least a first enzyme from a host cell, comprises cultivating the host cell in a sugar depleted cultivation environment comprising the host cell, water and a solid composition to produce the first enzyme for a cultivation time, where the first enzyme hydrolyzes a first pre-treated ligno-cellulosic biomass, the composition: comprises a complex sugar and a lignin; is obtained from a second pre-treated ligno-cellulosic biomass; and comprises a complex sugar of the second pre-treated ligno-cellulosic biomass and a lignin of the second pre-treated ligno-cellulosic biomass, and the ratio of the total amount of the complex sugars to the total amount of the lignin in the solid composition is less than the ratio of the total amount of the complex sugars to the total amount of the lignin in the second pre-treated ligno-cellulosic biomass. INDEPENDENT CLAIMS are included for:    (1) the enzyme or its mixture obtained by the above process; and    (2) a hydrolyzed ligno-cellulosic biomass hydrolyzed by the enzyme mixture.