NOVELTY - Extracting androstendione, comprises (i) dehydrating the fermentation liquid; (ii) extracting the dehydrated fermentation liquid oil phase and methanol for first time, and pumping the liquid to a pipeline mixer, and extracting by a pipe-type continuous ultrasonic extractor, and separating liquid-liquid mixed solution, to obtain oil phase A; (iii) extracting separated oil phase A and methanol for second time, pumping the liquid into pipeline mixer, extracting, and separating liquid-liquid mixed solution, to obtain oil phase B; and (iv) extracting oil phase B twice, and recovering methanol.    USE - The method is useful for extracting androstendione (claimed).    ADVANTAGE - The method has an extraction yield is more than 98%, and low energy consumption rate; can effectively improve the extraction efficiency; reduces the extraction stages and the usage amount of the solvent; guarantees the continuous and stable extraction process; and saves time.    DETAILED DESCRIPTION - Extracting androstendione, comprises (i) dehydrating the fermentation liquid, and controlling the water content below 1%; (ii) extracting the dehydrated fermentation liquid oil phase and methanol for the first time which are in the volume ratio of 1:2, and pumping the liquid into a pipeline mixer by using a measuring pump, and simultaneously extracting by a pipe-type continuous ultrasonic extractor, where the flow rate of the fermentation liquid oil phase is controlled at 1.5 m3/hour, and the flow rate of methanol is controlled at 3 m3/hour, and separating the liquid-liquid mixed solution, to obtain a clear liquid and an oil phase A; (iii) extracting the separated oil phase A and methanol for the second time which are in the volume ratio of 1:1, pumping the liquid again into the pipeline mixer by using the measuring pump, and simultaneously extracting by the pipe-type continuous ultrasonic extractor, where the flow rate of the fermentation liquid oil phase is controlled at 1.5 m3/hour, and the flow rate of methanol is controlled at 1.5 m3/hour, and separating the liquid-liquid mixed solution, to obtain a clear liquid and an oil phase B, where the content of androstenedione in the oil phase B is less than 2% of the androstenedione containing in the fermentation liquid oil phase; and (iv) extracting the oil phase B twice, and recovering methanol.