TECHNOLOGY FOCUS - BIOTECHNOLOGY - Recombinant Methods (Claimed): The method for producing polypeptide involves culturing a recombinant cell engineered to express the polypeptide under conditions in which the polypeptide is expressed. When the polypeptide comprises a signal sequence, the recombinant cell is cultured under conditions in which the polypeptide is secreted from the recombinant cell. The method further involves recovering the polypeptide from the cell culture. The step of recovering the polypeptide involves a step of centrifuging away cells and/or cellular debris; or a step of filtering away cells and/or cellular debris. Preferred Components: Each of the at least one substitution is selected from: Ala73 substitution selected from Ala73Gly and Ala73Ser; Tyr74 substitution that is Tyr74Leu; Val167 substitution that is Val167Ala; Thr219 substitution selected from Thr219Ala and Thr219Ser; Lys231 substitution that is Lys231Glu; Met246 substitution selected from Met46His and Met246Lys; Phe292 substitution selected from Phe292Ile and Phe292Val; Ser296 substitution that is Ser296Thr; Met325 substitution that is Met325Thr; Asn326 substitution that is Asn326Gly; Tyr399 substitution that is Tyr399Phe; Trp401 substitution that is Trp401Phe; Thr441 substitution that is Thr441Val; and Ala449 substitution that is Ala449Cys. Preferred Polypeptide: The polypeptide comprises Met246His substitution; or Ile216Val substitution; or Thr219Ala substitution; or comprises two substitutions selected from: Met246His, Ile216Val, and Thr219Ala; or comprises Met246His, Ile216Val, and Thr219Ala substitutions. The polypeptide further comprises at least one, at least two, at least three, at least four, at least five, at least six, at least seven, or eight substitutions selected from: Asp7His substitution; Asp154Asn substitution; Ile216Val substitution; Asp243His substitution; Asp302Arg substitution; Ser317His substitution; Glu365Gly substitution; and Val400Tyr substitution. The polypeptide comprises an amino acid sequence having at least 45%, at least 48%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 97% sequence identity to SEQ ID No. 379; or comprises an amino acid sequence having at least 45%, at least 48%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, or at least 95% sequence identity to SEQ ID No. 378 containing 447 amino acids. Preferred Composition: In the composition, the polypeptide represents at least 1% (preferably at least 5%, especially at least 25%) of all polypeptides in the composition. Preferred Nucleic Acid: The nucleic acid has at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 97% sequence identity to the nucleotide sequence of SEQ ID Nos. 1-198 or SEQ ID No. 381 e.g. SEQ ID No. 1 containing 1368 base pairs, SEQ ID No. 20 containing 1386 base pairs, SEQ ID No. 50 containing 1386 base pairs, SEQ ID No. 60 containing 1386 base pairs or SEQ ID No. 70 containing 1386 base pairs or SEQ ID No. 100 containing 1386 base pairs or SEQ ID No. 198 containing 1386 base pairs or SEQ ID No. 381 containing 1362 base pairs. The nucleic acid comprises the nucleotide sequence of SEQ ID Nos. 1-198 or SEQ ID No. 381. Preferred Vector: The vector further comprises an origin of replication. The vector further comprises a promoter sequence operably linked to the nucleotide sequence, where the promoter sequence is operable in yeast or the promoter sequence is operable in filamentous fungi.    TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Method: The method (M1) further involves recovering fermentable sugars, where the fermentable sugars comprise disaccharides or the fermentable sugars comprise monosaccharides. The method (M2) further involves prior to first step, pretreating the biomass. Preferred Components: The monosaccharides are produced by a beta -glucosidase in the composition or the fermentation broth.    TECHNOLOGY FOCUS - PHARMACEUTICALS - Preferred Components: The fermenting microorganism is a bacterium or yeast. The fermenting microorganism is a bacterium selected from Zymomonas mobilis, Escherichia coli and Klebsiella oxytoca; or is a yeast selected from Saccharomyces cerevisiae, Saccharomyces uvarum, Kluyveromyces fragilis, Kluyveromyces lactis, Candida pseudotropicalis, and Pachysolen tannophilus. The biomass is corn stover, bagasses, sorghum, giant reed, elephant grass, miscanthus, Japanese cedar, wheat straw, switchgrass, hardwood pulp, softwood pulp, crushed sugar cane, energy cane, or Napier grass. SPECIFIC PEPTIDES - 179 Sequences are specifically claimed as the polypeptide e.g. SEQ ID No. 267 containing 461 amino acids, SEQ ID No. 199 containing 461 amino acids, SEQ ID No. 200 containing 461 amino acids, SEQ ID No. 309 containing 461 amino acids and SEQ ID No. 310 containing 461 amino acids.    EXAMPLE - No suitable example given.