NOVELTY - Preparing lipstatin, comprises (i) preprocessing the fermentation broth; (ii) extracting filter cake using polar solvent; (iii) concentrating the filtered solution having lipstatin concentration of 15-30 g/l; (iv) extracting lipstatin by adding aqueous solution of organic solvent using an immiscible non-polar solvent, to obtain lipstatin containing non-polar extraction liquid; (v) concentrating lipstatin containing non-polar extraction liquid, adding polar solvent, to obtain lipstatin polarity extracting solution; (vi) removing insoluble impurities; (vii) crystallizing.    USE - The method is useful for preparing lipstatin (claimed).    ADVANTAGE - The method has total production yield of more than 80%, produces product with high content of 85.3%, purity of 96.5%, and good quality, and thus it performs the synthesis process and subsequently performs the hydrogenation process; shortens the preparation process; has easy operation procedure, and good recovering process; invests less on equipment; utilizes less amount of solvent; and is economical, and environmental friendly.    DETAILED DESCRIPTION - Preparing lipstatin, comprises (i) preprocessing the fermentation broth by adding a filter aid into the fermentation broth, and adjusting the acid pH to 4-5, stirring, and filtering to obtain filter cake; (ii) extracting the obtained filter cake using a polar solvent, stirring, and filtering to obtain a lipstatin solution; (iii) concentrating the resulting filtered solution having lipstatin concentration of 15-30 g/l, and adding drinking water to regulate the concentration of an aqueous solution of organic solvent to 50-80 vol.%; (iv) extracting lipstatin by adding aqueous solution of organic solvent using an immiscible non-polar solvent, to obtain lipstatin containing non-polar extraction liquid; (v) concentrating 100-200 g/l lipstatin containing non-polar extraction liquid, and adding polar solvent, to obtain lipstatin polarity extracting solution; (vi) stirring the lipstatin polarity extracting solution, cooling to 5 degrees C, maintaining the temperature, and filtering to remove the insoluble impurities, to obtain lipstatin clarification filtered liquid, and concentrating until the liquid gets dried, to obtain lipstatin oil; (vii) diluting the lipstatin oil by adding solvent until the concentration reaches 50-100 g/l, cooling, stirring to crystallize, and filtering to obtain white crystals of lipstatin.