NOVELTY - Bio-organic fertilizer is prepared by obtaining bagasse, preferably sugarcane bagasse as main raw material, and fermenting the sugarcane bagasse, animal excrement and pond sludge using effective microorganisms (EM). The fertilizer is prepared by mixing the sugarcane bagasse, animal excrement and pond sludge, stirring obtained mixture, dissolving sugar in purified water, adding EM to sugar solution to obtain EM culture liquid, mixing obtained mixture with the EM culture liquid and water, performing anaerobic fermentation, and drying and packing product.    USE - The bio-organic fertilizer is useful for crops.    ADVANTAGE - The fertilizer is cost-effectively prepared using EM having high purity that promotes degradation of organic matter, and rich in nutritional content, nitrogen, phosphorus and potassium, has improved quality, increases yield of crops, and reduces usage of chemical fertilizer.    DETAILED DESCRIPTION - Bio-organic fertilizer is prepared by obtaining bagasse, preferably sugarcane bagasse as main raw material, and fermenting the sugarcane bagasse, animal excrement and pond sludge using effective microorganisms (EM). The fertilizer is prepared by (a) obtaining 600 kg sugarcane bagasse, 200 kg animal excrement, 200 kg pond sludge, 1 kg EM, and 2 kg sugar, (b) mixing the sugarcane bagasse, animal excrement and pond sludge, and uniformly stirring obtained mixture, (c) dissolving sugar in 200-300 kg purified water in a container, then adding 1 kg EM to dissolved sugar solution, mixing and uniformly stirring obtained mixture, and sealing the container for 3-4 days to obtain 200-300 kg EM culture liquid, (d) mixing mixture obtained in step (b) with the EM culture liquid and suitable amount of water, and regulating water content of obtained mixture to 55-65%, (e) piling mixture obtained in step (d) to a height of 1 m and width of 2 m, covering the mixture tightly using plastic film or cloth, subjecting the mixture to anaerobic fermentation at 60 degrees C for 6-8 days, turning the mixture, covering the mixture tightly using plastic film or cloth, and again fermenting the mixture for 6-8 days, (f) drying fermented product at 60 degrees C until water content of the fermented product reaches less than 8-10%, and (g) packing obtained product.