TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Polypeptide: In the isolated or recombinant lipase polypeptide, the polypeptide has a mutation at Gly181 and Ser238 and has improved thermostability compared to a polypeptide comprising SEQ ID NO: 2. Mutation at Gly181 is Gly181Cys. Mutation at Ser238 is Ser238Cys. The polypeptide is at least 80%, 85%, or 95% identical to SEQ ID NO: 15 and having Cys181 and Cys238; or the polypeptide comprises SEQ ID NO: 15. It further comprises a mutation at a residue selected from Arg33, Leu64, Ala70, Glys202, Lys208, Gly266 or its combination and where the lipase has improved methanol tolerance compared to a polypeptide of SEQ ID NO: 2. The polypeptide further comprises a mutation selected from Arg33Thr, Leu64Ile, Ala70Thr, Gly202Glu, Lys208Asn, Gly266Ser and its combination. It comprises the mutations Arg33Thr, Leu64Ile, Ala70Thr, Gly202Glu or Gly202Asp, Lys208Asn, and Gly266Ser. It further comprises a mutation at a residue selected from Leu13, Asn17, Phe40, Val61, EGlu67, Ala70, Ser95, Arg117, Met119, Thr138, Ser141, Ser144, Gly145, Asp149, Ala153, Gly206, Glu207, Asn223, Phe225, Gly243, Leu245, Ile255, Tyr267, Asp270, Val272, Gln277 and its combination. The polypeptide has mutations selected from: (a) Phe225Leu and Gln277Leu; (b) NAsn17S, Met119Ile, Phe225Leu, Asp270Asn and Gln277Leu; and (c) Asn17Ser, Glu67A, Met119Ile, Ala153Val, Phe225Leu, Ile255Phe, Asp270Asn and Gln277Leu. It is at least 80% identical to SEQ ID NO: 2 and has mutations selected from: (a) Leu64Ile, Ala70Thr, Gly181Cys, Lys208Asn, Phe225Leu, Ser238Cys and Gln277Leu; (b) Asn17Ser, Leu64Ile, Ala70Thr, Met119Ile, Gly181Cys, Gly202Glu, Lys208Asn, Phe225Leu, Ser238Cys, Gly266Ser, Asp270Asn and Gln277Leu; and (c) Asn17Ser, Arg33Thr, Leu64Ile, Glu67Ala, Ala70Thr, Met119Ile, Ala153Val, Gly181Cys, Gly202Glu, Lys208Asn, Phe225Leu, Ser238Cys, Ile255Phe, Gly266Ser, Asp270Asn and Gln277Leu. The polypeptide comprises sequence selected from SEQ ID NO: 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, or 85. It further comprises 1-20 conservative amino acid substitutions. Preferred Polynucleotide: The polynucleotide is selected from SEQ ID NO: 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, and 84. Sequences not defined here may be defined at ftp://ftp.wipo.int/pub/published_pct_sequences/publication. Preferred Vector: The vector is an expression vector. Preferred Cell: The cell is prokaryotic or eukaryotic cell. EXAMPLE - No suitable example given.