NOVELTY - An isolated variant (v1) of a parent cellobiohydrolase (CBH) enzyme, having cellulase activity, at least 80% sequence identity to specified amino acid sequence, and significantly increased melting temperature (Tm) as compared to the parent CBH enzyme, is new.    USE - In detergent composition and feed additive; for producing cell culture supernatant with cellulases and/or hemicellulases; and for hydrolyzing a cellulosic substrate of a lignocellulosic biomass and is selected from grass, switch grass, cord grass, rye grass, reed canary grass, miscanthus, sugar-processing residues, sugarcane bagasse, agricultural wastes, rice straw, rice hulls, barley straw, corn cobs, cereal straw, wheat straw, canola straw, oat straw, oat hulls, corn fiber, stover, soybean stover, corn stover, forestry wastes, wood pulp, recycled wood pulp fiber, paper sludge, sawdust, hardwood and/or softwood (claimed), particularly for converting cellulosic materials to monosaccharides, disaccharides, and polysaccharides. The cellulase composition containing CBH variant is used in ethanol production.    ADVANTAGE - The cellobiohydrolase (CBH) variants have improved properties such as, but are not limited to: altered temperature-dependent activity profiles, thermostability, pH activity, pH stability, substrate specificity, product specificity and chemical stability. The CBH variants with improved performance under conditions of thermal stress can be used to increase cellulase activity in assays carried out at high temperatures (e.g. temperatures at which the parent CBH would perform poorly), allowing a user to reduce the total amount of CBH employed (as compared to using the parent CBH). Other improved properties of CBH variant polypeptides can be exploited in cellulase assays, including CBH variants having altered pH optima, increased stability or activity in the presence of surfactants, increased specific activity for a substrate, altered substrate cleavage pattern, and/or high level expression in a host cell of interest. Thus, CBH variant polypeptides are used in detergent compositions that exhibit enhanced cleaning ability, function as a softening agent and/or improve the feel of cotton fabrics (e.g. stone washing or biopolishing), in compositions for degrading wood pulp into sugars (e.g. for bio-ethanol production), and/or in feed compositions. The isolation and characterization of CBH variants provides the ability to control characteristics and activity of such compositions.    DETAILED DESCRIPTION - An isolated variant (v1) of a parent cellobiohydrolase (CBH) enzyme, having cellulase activity, at least 80% sequence identity to amino acid sequence of 497 amino acids (SEQ ID NO: 3) as given in the specification, and significantly increased melting temperature (Tm) as compared to the parent CBH enzyme, is new. INDEPENDENT CLAIMS are included for the following:    (1) an isolated polynucleotide comprising a polynucleotide sequence encoding the variant (v1) of a parent CBH polypeptide;    (2) a vector comprising the isolated polynucleotide;    (3) a host cell comprising the isolated polynucleotide, the vector, or the expression vector;    (4) producing variant CBH polypeptide;    (5) a detergent composition comprising a surfactant and the variant CBH polypeptide;    (6) a feed additive comprising the variant CBH polypeptide;    (7) hydrolyzing a cellulosic substrate, involving: contacting the substrate with the isolated variant CBH polypeptide; and/or contacting the substrate with the host cell;    (8) a cell culture supernatant comprising the CBH variant; and    (9) producing (p1) a cell culture supernatant comprising CBH variant, involving: culturing the host cell under suitable conditions to express the CBH variant; and collecting the cell culture supernatant of the culture.