NOVELTY - Biodiesel preparation involves extracting liquid by slicing fresh jerusalem artichoke inulin, then drying and crushing the material, and then passing through 100 mesh sieve to obtain artichoke. Jerusalem artichoke is dissolved in distill water. The obtained material 4000 revolution per minute for 10 minutes, and then supernatant is collected to obtain the chrysanthemum sugar extraction liquid. The pH value of prepared inulin extract is set at 4.6, then sugar and cutting enzyme is added at 55 degrees C and 5.5 pH value for 8 hours.    USE - Method for preparing biodiesel (claimed).    DETAILED DESCRIPTION - Biodiesel preparation involves extracting liquid by slicing fresh jerusalem artichoke inulin, then drying and crushing the material, and then passing through 100 mesh sieve to obtain artichoke. Jerusalem artichoke is dissolved in distill water. The obtained material 4000 revolution per minute for 10 minutes, and then supernatant is collected to obtain the chrysanthemum sugar extraction liquid. The pH value of prepared inulin extract is set at 4.6, then sugar and cutting enzyme is added at 55 degrees C and 5.5 pH value for 8 hours. Enzymolysis of extracted liquid is carried out at 115 degrees C for 30 minutes. Culture medium is prepared, and then sheath-shaped silk spore yeast is inoculated (10%) at 30 degrees C, then it is rotated at 200 revolutions per minute. The obtained material is dried. Prepared fat yeast Trichosporon cutaneum is transferred into a single neck flask. Sodium hydroxide is dissolved in anhydrous methanol to prepare a methanol solution of catalyst for leather-like yeast Trichosporon cutaneum. Catalyst is added to the flask, and the flask is heated in microwave oven for 1.0-3.0 minutes. Hydrochloric acid is added to the flask. Reaction mixture is placed in a funnel. The obtained material is distilled at 70 degrees C under atmospheric pressure. After distillation of ester phase the obtained material is placed in a separatory funnel, and then placed in a microwave oven for l0 minutes. Repeat the distillation process, and then heated the product. Anhydrous sodium sulfate is added to the material to remove residual moisture to obtain pale yellow biodiesel.