TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation: No preparation method is given. Preferred Components: The microorganism further comprises a butanol biosynthetic pathway. The microorganism comprises a genetic modification in an endogenous polynucleotide encoding a polypeptide having sporulation activity, where the polypeptide comprises apical membrane antigen 1, apical membrane antigen 1, APG1, APG2, APG7, APG9, APG10, APG12, APG13, ADP-ribosylation factor domain protein 1, ATP10, AUT1, AUT7, AUT10, bisindolyl maleimide 1, BRE1, budding uninhibited by benzimidazoles 3, nuclear cap-binding protein subunit 2, cell division control protein 10, cell division control protein 40, CTD kinase 1, CTD kinase subunit beta, cyclin-dependent kinase 8, DOA4, DHH1, ERV14, GCN5, mannosyl-oligosaccharide glucosidase, generative cell specific 1, HOP2, HPR1, IME1, IME2, IMP2, ISC1, MLS1, MUM2, MON1, meiotic plaque component protein 54, MSO1, N-acetyltransferase 1, NDT80, NGG1, NPR2, PAF1, phosphoenolpyruvate carboxykinase 1, PEP3, PEP7, PEP12, PPA1, RAD6, RAM1, RIM4, RIM11, SIN3, SLG1, SLX8, SMA2, sucrose non-fermenting 1, sucrose non-fermenting 2, sucrose non-fermenting 6, superoxide dismutase 2, SPO1, SPO11, SPO14, SPO20, SPO21, SPO71, SPO73, SPO74, SPO75, SPO77, SSO1, SPT20, sporulation-specific protein 1, SWI3, TUP1, ubiquitin-like modifier activating enzyme 14, UME6, vacuolar protein 8, vacuolar morphogenesis protein 3, vacuolar morphogenesis protein 6, vacuolar morphogenesis protein 7, V-type proton ATPase subunit B, V-type proton ATPase subunit 6, V-type proton ATPase subunit 13, vacuolar protein sorting-associated protein 13, vacuolar protein sorting-associated protein 30, vacuolar protein sorting-associated protein 41, YPT7, YBL100C, YGL218W, YGR226Q, YKL118W, YMR158W-A, YNL296W, YOR135C and YPL183W-A, preferably IME1. The genetic modification results in less than 5%, preferably less than 0.01% of sporulation efficiency. The butanol biosynthetic pathway is an isobutanol biosynthetic pathway. The microorganism is a diploid or tetraploid. The microorganism comprises the genetic modification in the endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity, where the polypeptide comprises pyruvate decarboxylase (PDC)-1, PDC-5, and/or PDC-6. The microorganism comprises an insertion, deletion, mutation, and/or substitution in one or more endogenous polynucleotides encoding fos-related antigen 2, glycerol-3-phosphate dehydrogenase 2,3-hydroxybutyrate dehydrogenase, type 1, phosphodiesterase type 1, and Yersinia multiple regulator 226C. The pH of microorganism is less than or equal to 2. The microorganism comprises the genetic modification in an endogenous polynucleotide encoding a polypeptide having pheromome activity, where the polypeptide comprises BEM1, CDC24, CDC42, DIG1, DIG2, FAR1, FUS3, GPA1, KSS1, STE2, STE3, STE4, STE5, STE7, STE11, STE12, STE18, STE20, and STE50.    TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Components: The carbon substrate is glucose, fructose, and/or sucrose. EXAMPLE - No suitable example given.