NOVELTY - Producing sophorolipid using waste molasses and oil, by inoculating Torulopsis bombicola (ATCC 22214) in yeast extract peptone dextrose (YPD) agar slant, culturing medium, adding seed liquid medium to flask, inoculating bacterial suspension into sterilized seed liquid medium, culturing medium, inoculating seed liquid in fermentation culture medium comprising sterilized molasses and waste oil, adding seed liquid to sterilized culture medium, performing fermentation and extracting sophorolipid from fermented liquid using ethyl acetate.    USE - The method is useful for production of sophorolipid (claimed).    ADVANTAGE - The method enables production of the sophorolipid at a yield of 30-100 g/l and effective utilization of molasses and oil discarded as waste, and hence environmentally-friendly, as pollution caused by discharge of the waste oil is also reduced. The method significantly reduces production cost of the sophorolipid, as complex devices or equipments are not required and the fermentation process is simple and easy. The method has good development, application and popularization prospects.    DETAILED DESCRIPTION - Method for producing sophorolipid using waste molasses and oil as carbon source by fermentation process, involves (a) inoculating Torulopsis bombicola (ATCC 22214) in yeast extract peptone dextrose (YPD) agar slant culture medium, diluting the culture medium with distilled water up to 1 l, maintaining pH of the diluted culture medium at 6, performing culturing process at 30 degrees C for 2-3 days and eluting cultured medium using 0.85 %mass physiological saline solution to obtain bacterial suspension, (b) adding 100 ml seed liquid medium comprising glucose, yeast extract and urea to 500 ml flask, sterilizing the medium at 121 degrees C for 20 minutes, cooling sterilized medium, inoculating 3-5 vol.% bacterial suspension as inoculum into the seed liquid medium and performing culturing process under shaking conditions by shaker at 25-32 degrees C and 120-180 revolutions per minute (rpm) for 24 hours to obtain seed liquid, (c) inoculating the seed liquid in 5 l fermentation tank containing fermentation culture medium comprising sterilized molasses and waste oil as fermentation substrate, making up the volume of the fermentation substrate up to 3 l using tap water, adjusting pH of the medium at 6, sterilizing the fermentation medium at 121 degrees C for 20 minutes, cooling the seed liquid, adding the seed liquid to sterilized culture medium, and performing fermentation at 25-32 degrees C and dissolved oxygen concentration of 20-60%, and (d) extracting the sophorolipid from obtained fermented liquid using organic solvent by adding ethyl acetate to the fermented liquid in an equal volume, and removing the organic solvent from obtained liquid extract by distillation under reduced pressure conditions to obtain the sophorolipid.