NOVELTY - A micro-fluidic chip's monolithic column preparing method involves preparing PDMS micro-fluidic chip, using soft lithography's standard micro-machining method for preparing PDMS micro-fluidic chip, followed by preparing porous monolithic column solution by mixing glycidyl methacrylate as monomer, ethylene glycol dimethacrylate as cross-linking agent, dioctyl phthalate as pore forming agent with dimethylolpropionic acid as initiator ultrasonically to obtain mixed product, using nitrogen in monolithic column solution to remove oxygen. The column solution is placed in ice bath.    USE - Method for preparing micro-fluidic chip's monolithic column for use in Raman detection of organic small molecule 2-pyridine (claimed), serum marker detection, gas marker detection, environmental monitoring and food safety field.    ADVANTAGE - The method enables preparing micro-fluidic chip's monolithic column with reduced energy consumption and better flexibility in a convenient and inexpensive manner.    DETAILED DESCRIPTION - A micro-fluidic chip's monolithic column preparing method involves preparing PDMS micro-fluidic chip, using soft lithography's standard micro-machining method for preparing PDMS micro-fluidic chip, followed by preparing porous monolithic column solution by mixing glycidyl methacrylate as monomer, ethylene glycol dimethacrylate as cross-linking agent, dioctyl phthalate as pore forming agent at a weight ratio of 2:1:7-1:2:2 with 1-5 wt.% dimethylolpropionic acid as initiator ultrasonically to obtain mixed product, using nitrogen in monolithic column solution to remove oxygen. The column solution is placed in ice bath. The monolithic column solution is injected to micro-fluidic chip in a pipeline, followed sealing by using sealing film, fixing optical mask plate to the chip and pipeline area to control exposure, placing entire device under UV light exposure, removing sealing film inlet and outlet, washing a microfluidic channel by injecting methanol by using a syringe pump, removing porogen and unreacted monolith solution, and washing using deionized water. An INDEPENDENT CLAIM is also included for a micro-fluidic chip's monolithic column.