TECHNOLOGY FOCUS - BIOLOGY - Preferred Method: (I) produces a metabolite in the production of n-butanol and/or producing n-butanol, where the metabolite and/or n-butanol is/are produced through a malonyl-CoA dependent pathway comprising an oxygen tolerant CoA-acylating aldehyde dehydrogenase. Preferred Components: The organism comprises expression or elevated expression of an enzyme and expression of at least one heterologous, or the over expression of at least one endogenous, target enzyme that convert acetyl-CoA to malonyl-CoA, malonyl-CoA to acetoacetyl-CoA, and at least one enzyme that converts acetoacetyl-CoA to (R)- or (S)-3-hydroxybutyryl-CoA, (R)- or (S)-3-hydroxybutyryl-CoA to crotonyl-CoA, crotonyl-CoA to butyryl-CoA, butyryl-CoA to butyraldehyde and butyraldehyde to 1-butanol. (I) comprises a metabolic pathway for the production of 1-butanol that is an NADPH dependent pathway. The photoautotrophic or photoheterotrophic microorganism is engineered to express or overexpress one or more polypeptides that convert acetyl-CoA to malonyl-CoA and malonyl-CoA to acetoacetyl-CoA. The polypeptides comprises a nphT7 polypeptide comprising at least 90% identity to a sequence of SEQ ID NO: 18 and having acetoacetyl-CoA synthase activity. (I) is engineered to express an acetyl-CoA carboxylase. The acetyl-CoA carboxylase comprises a sequence that is at least 90% identical to a sequence of SEQ ID NO: 2. The microorganism further expresses or overexpresses one or more enzymes that carries out a metabolic function comprising (a1) converting acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA, (b1) converting acetoacetyl-CoA to (S)-3-hydroxybutyryl-CoA, (c1) converting (R)-3-hydroxybutyryl-CoA to crotonyl-CoA, (d1) converting (S)-3-hydroxybutyryl-CoA to crotonyl-CoA, (e1) converting crotonyl-CoA to butyryl-CoA, and (f1) converting butyryl-CoA to butyraldehyde and butyraldehyde to 1-butanol. (I) comprises an NADPH dependent metabolic pathway: that converts (i) acetyl-CoA to malonyl-CoA, (ii) malonyl-CoA to acetoacetyl-CoA, (iii) acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA, (iv) (R)-3-hydroxybutyryl-CoA to crotonyl-CoA, (v) crotonyl-CoA to butyryl-CoA, (vi) butyryl-CoA to butyraldehyde, and (vii) butyraldehyde to 1-butanol; and that converts (i) acetyl-CoA to acetoacetyl-CoA, (ii) acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA, (iii) (R)-3-hydroxybutyryl-CoA to crotonyl-CoA, (iv) crotonyl-CoA to butyryl-CoA, (v) butyryl-CoA to butyraldehyde, and (vi) butyraldehyde to 1-butanol. (I) is a photoautotroph or photoheterotroph microorganism that is engineered to express or overexpress an acetyl-CoA carboxylase and an acetoacetyl-CoA synthase and further expresses or overexpresses one or more enzymes comprising: (a) acetoacetyl-CoA reductase, (b) enoyl-CoA hydratase, (c) crotonyl-CoA reductase, and (d) an oxygen tolerant CoA-acylating aldehyde dehydrogenase; (a), (b), (c) trans-2-enoyl-CoA reductase and (d); (a), (b), (c) trans-2-enoyl-CoA reductase, and (d) oxygen tolerant CoA-acylating aldehyde dehydrogenase and 1,3-propanediol dehydrogenase; (a) hydroxybutyryl CoA dehydrogenase, (b) crotonase, (c) trans-2-enoyl-CoA reductase, and (d) oxygen tolerant CoA-acylating aldehyde dehydrogenase and 1,3-propanediol dehydrogenase. The photoautotrophic or photoheterotrophic organism that comprises a reduction, disruption or knockout of at least one gene encoding an enzyme that competes with a metabolite necessary for the production of a desired higher alcohol product or which produces an unwanted product. The photoautotrophic or photoheterotrophic organism is engineered to disrupt, delete or knockout one or more genes encoding a polypeptide or protein consisting of: (i) an enzyme that catalyzes the NADH dependent conversion of pyruvate to D-lactate (e.g. Lactate dehydrogenase A (LdhA)); (ii) an enzyme that promotes catalysis of fumarate and succinate interconversion (e.g. fumarate reductase iron-sulfur subunit (frdBC)); (iii) an oxygen transcription regulator; and (iv) an enzyme that catalyzes the conversion of acetyl-coA to acetyl-phosphate (e.g. phosphotransacetylase (pta)), where (I) comprises a disruption, deletion or knockout of a combination of an alcohol/acetoaldehyde dehydrogenase and one or more of (i)- (iv). (I) is engineered to express one or more subunits of acetyl-coA carboxylase (AccABCD) that converts acetyl-CoA to malonyl-CoA, or engineered to express of over express one or more genes consisting of nphT7, phaB, phaJ, ter, pdup, or yqhD, where the microorganism produces n-butanol. (I) further comprises expressing or over expressing AccABCD, and expresses a polypeptide having at least 90-100% identity to a sequence of SEQ ID NO: 2 (AccABCD), SEQ ID NO: 18 (nphT7), SEQ ID NO: 30 (phaB), SEQ ID NO: 28 (phaJ), SEQ ID NOs: 23-26 (ter), SEQ ID NO: 34 (PduP) and SEQ ID NO: 32 (yqhD). (I) comprises an expression profile consisting of: (a) AccABCD, nphT7, PhaB, PhaJ, Ter, PduP, and YqhD; (b) nphT7, PhaB, PhaJ, Ter, PduP, and YqhD; (c) AccABCD, nphT7, PhaB, PhaJ, ccr, PduP, and YqhD; (d) nphT7, PhaB, PhaJ, cassette chromosome recombinase (ccr), PduP, and YqhD; (e) AccABCD, nphT7, Clostridium acetobutylicum, 3-hydroxybutyryl-CoA dehydrogenase (hbd), (C. acetobutylicum), crotonase (crt), Ter, PduP, and YqhD; or (f) nphT7, hbd, crt, Ter, PduP, and YqhD. The vector is a plasmid or an expression vector, where the above sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication.    TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preparation: No preparation method is given. EXAMPLE - No suitable example given.