NOVELTY - Preparation of anti-cropping bio-fertilizer involves, (a) preparation of long cylindrical fermentation tank with internal cavity of seal belt in longitudinal direction, and sealed chamber with a heat insulation device, humidification moisturizing device and stir aerator, and connecting the sealed chamber through an exhaust duct, (b) mixing fermented manure, plant waste and bio-fermentation agent, and (c) transferring mixture to tank, sealing the mixture, fermenting, generating fermentation odor and transporting odor through pipe, and processing the mixture.    USE - The method is useful for preparation of anti-cropping bio-fertilizer (claimed).    ADVANTAGE - The anti-cropping bio-fertilizer has excellent cropping effect, and increases fertilizer effect and improves yield. The method is simple, and cost-effective, changes waste into valuable product, solves problem by reducing waste to environment, and improves the fermentation by adding biological material.    DETAILED DESCRIPTION - Preparation of anti-cropping bio-fertilizer involves, (a) preparation of long cylindrical fermentation tank with internal cavity of seal belt in longitudinal direction, and sealed chamber with a heat insulation device, humidification moisturizing device and stir aerator, and connecting the sealed chamber through an exhaust duct with suction, (b) mixing fermented manure, plant waste, and bio-fermentation agent in weight ratio of 200-250:100-125:0.1-0.5, where pig manure moisture content and pH are 40-60% and 6.5-7.5 respectively, plant waste is one or more of crop straw, rice husk, bran, or sawdust, and weight ratio, moisture content, and pH of carbon and nitrogen are 40-50:1, 40-60%, and 6.5-7.5 respectively, and bio-fermentation agents are beer yeast bacteria, Bacillus subtilis, lactic acid bacteria, and Streptomyces in volume ratio of 2-3:3-4:3-4:4-5, and (c) transferring mixture obtained in step (b) to fermentation tank in step (a), fully sealing the mixture, fermenting the mixture at 50-60 degrees C, under 70-80% humidity and 70 ?? 80%, 0.5-1 MPa pressure for 1 hour, generating fermentation odor during process of fermentation, transporting through pipe to the exhaust gas, and processing the mixture for 10-12 hours. The preparation of beer yeast bacteria, involves using 10 g mother culture, 20 g peptone, 20 g glucose, and 1000 ml distilled water, and fermenting bacteria at 28-38 degrees C for 24-48 hours under aerobic fermentation. The preparation of B.subtilis, involves using 15 g peptone, 0.5 g beef extract, 20 g glucose, 5 g sodium chloride, and 1000 ml distilled water, and fermenting the culture at 30-36 degrees C for 48-72 hours under aerobic fermentation. The preparation of lactic acid bacteria, involves using 70 ml desired wort, 10 g beef extract, 5 g yeast extract, 5 g soy protein powder, and 1000 ml water, and fermenting the culture at 38-42 degrees C for 48-72 hours under aerobic fermentation, where pH of medium is 6.5. The preparation of Streptomyces, involves using 1 g maltose, 1 g glucose, 1 g sucrose, 3 g soluble starch, 2 g peptone, 10 g beef extract, 5 g yeast extract, 2 g soybean meal, and 1000 ml water, and fermenting the culture at 38-42 degrees C for 48-72 hours under aerobic fermentation, where pH of medium is 6.5.