NOVELTY - A shuttle vector for transferring genetic material between Caldicellulosiruptor spp. and an amplification cell, comprising origin of replication sequence from the amplification cell, origin of replication for Caldicellulosiruptor spp., selectable marker for the amplification cell, and a heterologous coding sequence complements a functional deletion in the Caldicellulosiruptor spp. genome, is new.    USE - The shuttle vector is useful for transferring genetic material between Caldicellulosiruptor spp. and an amplification cell for producing genetically modified Caldicellulosiruptor spp. microbe with increased biosynthesis of a bioproduct such as biofuel, preferably ethanol (all claimed). The vector is also useful for analyzing important biological functions unique to Caldicellulosiruptor spp., and for producing other commercially or industrially relevant chemicals.    ADVANTAGE - The shuttle vector extends breadth of biomass substrates, on which microbes can grow and metabolic pathways are engineered, facilitates extension of genetic methods to a number of other Caldicellulosiruptor species, simplifies method for genetic manipulation of members of the Caldicellulosiruptor and allows metabolic engineering for the direct conversion of plant biomass to liquid fuels such as ethanol. The genetically modified Caldicellulosiruptor spp. microbe exhibits increased biosynthesis of the bioproduct compared to a wild type control when grown on lignocellulosic biomass.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) cell comprising shuttle vector; (2) method (M1) for introducing the shuttle vector into a cell; (3) genetically modified Caldicellulosiruptor spp. microbe engineered for increasing biosynthesis of a bioproduct, exhibiting an increase in biosynthesis of the bioproduct compared to a wild type control; and (4) method for growing the genetically modified Caldicellulosiruptor spp. microbe on a feed stock to biosynthesize the bioproduct.