TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Acid: In the nucleic acid, the 5' UTR is from a non-fungal homolog of a highly expressed fungal gene that is conserved between fungi and non-fungi; or the 5' UTR is from a non-fungal homolog of a highly expressed Trichoderma reesei gene. The Trichoderma reesei gene is an elongation factor Tu; heat shock protein (Hsp)-70; beta-transducin repeat (WD40); glyceraldehyde 3-phosphate dehydrogenase; thioredoxin; ATP synthase; actin; calreticulin; E1-E2 ATPase; thiamine pyrophosphate enzyme; elongation factor 5A; ubiquitin; ribosomal protein S3A; thiamine (Thi)-4; Thi5; Histone; Hsp90; NAD(P) transhydrogenase; glutamine synthetase; aconitase; elongation factor 1; ribosomal protein S13/S18; ribonucleotide reductase M1 (RRM1); ubiquitin; thioredoxin; ATPase; or acetohydroxy acid isomeroreductase gene. The non-fungal homolog is from a species of phylum Euglenozoa, Percolozoa, Loukozoa, Metamonada, Cercozoa, Heterokontophyta, Haptophyta, Cryptophyta, Alveolata, Apicomplexa, Chromerida, Ciliophora, Dinoflagellata, Retaria, Foraminifera, or Radiolaria. It is from Dictyostelium purpureum, Daphnia pulex, Ostreococcus RCC809, Aureococcus anophagefferens, or Arabidopsis lyrata. The 5' UTR comprises a nucleotide sequence having at least 70%, 75%, 80%, 85%, or 90% sequence identity to any one of at least fully defined 90 bp (SEQ ID NOS: 4-67) given in the specification; or the 5' UTR comprises the nucleotide sequence of any one of SEQ ID NOS: 4-67. The 5' UTR is at least 30 nucleotides in length; the 5' UTR is 30 to 500 nucleotides in length; the 5' UTR is at least 50 nucleotides in length; the 5' UTR is at least 75 nucleotides in length; or the 5' UTR is at least 100 nucleotides in length. The 3' UTR comprises a polyadenylation signal. Nucleic acid further comprises between the first polypeptide coding sequence and the 3' UTR an internal ribosome entry site (IRES) and a second polypeptide coding sequence. The first polypeptide is a filamentous fungal polypeptide. It is a Trichoderma reesei polypeptide. The first polypeptide is yeast, mammalian or bacterial polypeptide. It comprises a signal sequence. The first polypeptide is a beta -glucosidase, where the beta -glucosidase comprises the amino acid sequence of fully defined 869 amino acids (SEQ ID NO: 3) given in the specification. The promoter is a mammalian viral promoter, where the mammalian viral promoter is a Rous sarcoma virus (RSV) long terminal repeat (LTR) promoter, a cytomegalovirus immediate early gene (CMV) promoter, a Simian virus early (SV40) promoter, or an adenovirus major late promoter. It is not an SV40 promoter. The mammalian viral promoter is a CMV promoter. The promoter is a plant viral promoter, where the plant viral promoter is a cauliflower mosaic virus (CaMV) promoter, a Commelina yellow mottle virus (CoYMV) promoter, a Figwort Mosaic Virus (FMV) promoter, or a cassava vein mosaic virus (CsVMV) promoter. The plant viral promoter is a CaMV 35S promoter, or CoYMV promoter. Alternatively, the promoter is a fungal promoter, where the promoter is obtained from a gene selected from Aspergillus oryzae TAKA amylase, Rhizomucor miehei aspartic proteinase, Aspergillus niger neutral alpha-amylase, Aspergillus niger acid stable alpha-amylase, Aspergillus niger or Aspergillus awamori glucoamylase (glaA), Rhizomucor miehei lipase, Aspergillus oryzae alkaline protease, Aspergillus oryzae triose phosphate isomerase, Aspergillus nidulans acetamidase, Fusarium venenatum amyloglucosidase, Fusarium venenatum Daria, Fusarium venenatum Quinn, Fusarium oxysporum trypsin-like protease, Trichoderma reesei beta -glucosidase, Trichoderma reesei cellobiohydrolase I, Trichoderma reesei cellobiohydrolase II, Trichoderma reesei endoglucanase I, Trichoderma reesei endoglucanase II, Trichoderma reesei endoglucanase III, EXAMPLE - No suitable example given.