TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Components: The emulsifier is chosen from Tween 60 (RTM: Polysorbate 60), Tween 80 (RTM: Polysorbate 80), Span 60 (RTM: Sorbitan monostearate) and Span 80 (RTM: Sorbitan oleate). Preferred Medium: The seed culture medium (pH 6-8) comprises 10-15 g/l glucose, 0.5-0.6 g/l dipotassium phosphate, 0.5-0.6 g/l magnesium sulfate heptahydrate, 0.04-0.05 g/l ammonium ferrous sulfate hexahydrate, 5-5.3 g/l calcium carbonate and 2-2.5 g/l citric acid. The fermentation culture medium (pH 6-8) comprises 20-25 g/l glucose, 20-25 g/l peptone, 15-20 g/l soybean meal, 0.7-0.8 g/l dipotassium phosphate, 0.6-0.7 g/l magnesium sulfate heptahydrate, 0.04-0.05 g/l ammonium ferrous sulfate hexahydrate, 5-5.5 g/l calcium carbonate and 2-2.5 g/l citric acid. Preferred Method: The method involves adding 1.5-3 %mass plant sterol, 0.1-0.5 %mass Tween 60 (RTM: Polysorbate 60), 0.5-2 %mass Tween 80 (RTM: Polysorbate 80), 0.5-1 %mass Span 60 (RTM: Sorbitan monostearate) and 0.5-2 %mass Span 80 (RTM: Sorbitan oleate) in fermentation culture medium. The method involves emulsifying the plant sterol at 50-70 degrees C. The concentration of the M.smegmatis strains is 107-109 cells/ml in the inoculum size of 5-10% in the seed culture medium placed in the culture tank. The method involves inoculating 20-50% inoculum size of the M.smegmatis in fermentation culture medium in the fermentation tank, where the optical density of the inoculation culture is 15-20 at 600 nm. The method involves fermenting at 25-30 degrees C under the pH of 6-8 with the dissolved oxygen content of 20-40% and rotating speed of 150-200 revolutions/minute (rpm) for 80-120 hours. The method involves extracting the finished product by filtering the fermentation broth to obtain filter cake, dissolving the filter cake using at least twice the amount of ethanol or acetone, extracting, filtering and distilling to obtain a crude product, dissolving the crude product in at least 4 times quantity of dichloromethane or chloroform, then extracting, bleaching, filtering, distilling, adding twice the volume of hexane or toluene and performing crystallization to obtain purified product.