NOVELTY - Isolated variant of a parent cellobiohydrolase (CBH) enzyme having cellulase activity, at least 80% sequence identity to SEQ ID NO:3 (a 497 amino acid sequence fully defined in the specification) and at least one improved property over the parent CBH enzyme comprising expression (protein content determination), phosphoric acid swollen cellulose (PASC) hydrolysis assay, PASC hydrolysis assay in the presence of EG2, PASC hydrolysis assay after heat incubation, hydrolysate PCS (whPCS) Assay, dilute ammonia corn cob (daCC) assay, and dilute ammonia corn stover (daCS) assay, is new.    USE - The isolated variant CBH is useful in a detergent composition and a feed additive. The isolated variant CBH polypeptide or the host cell is useful for hydrolyzing a cellulosic substrate such as a lignocellulosic biomass including grass, switch grass, cord grass, rye grass, reed canary grass, miscanthus, sugar-processing residues, sugarcane bagasse, agricultural wastes, rice straw, rice hulls, barley straw, corn cobs, cereal straw, wheat straw, canola straw, oat straw, oat hulls, corn fiber, stover, soybean stover, corn stover, forestry wastes, wood pulp, recycled wood pulp fiber, paper sludge, sawdust, hardwood and/or softwood (all claimed) to produce ethanol used in lieu of petrol, where the detergent composition is useful for cleaning textiles or cotton fabrics.    ADVANTAGE - The isolated variant CBH: has improved properties such as temperature-dependent activity profiles, thermostability, pH activity, pH stability, substrate specificity, product specificity, and chemical stability; ensures that the detergent composition exhibits enhanced cleaning performance and improves the feel (softness) and appearance of the cotton fabrics; and ensures that the ethanol can be produced in an eco-friendly manner.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for:    (1) an isolated polynucleotide comprising a polynucleotide sequence encoding a variant of the parent CBH polypeptide;    (2) a vector comprising the isolated polynucleotide;    (3) a host cell comprising the isolated polynucleotide, the vector, or the expression vector;    (4) a method-I for producing a variant CBH polypeptide, comprising culturing the host cell in a culture medium under suitable conditions;    (5) a detergent composition comprising a surfactant and the variant CBH polypeptide;    (6) a feed additive comprising the variant CBH polypeptide;    (7) a cell culture supernatant comprising a CBH variant; and    (8) a method-II for producing the cell culture supernatant, comprising culturing the host cell under suitable conditions to express the CBH variant, and collecting the cell culture supernatant of the culture.