TECHNOLOGY FOCUS - BIOLOGY - Preparation (Claimed): Preparation of the strains of microalgae, involves: (a) isolating the microalgae from low quality waters; (b) culturing the microalgae in algae culture medium (AIM); (c) adapting the microalgae by culturing in medium comprising algal isolation medium, low quality water containing heavy metals of 100-1000 ppm, hydrocarbons of 0.001-2% and high concentration of salt of up to 3%; (d) growing the microalgae in the culture medium for twelve cycles; (e) treating the microalgae with mutagenzing agents; (f) obtaining mutagenized microalgae; (g) isolating the protoplast from mutagenized microalgae of step (c); (h) shuffling the protoplast obtained step (d) for fusion using 60% polyethylene glycol (PEG)-6000; (i) culturing the microalgae of the fused protoplast in AIM media containing 0.5 M osmotic agent; (j ) carrying out the steps (d)-(f) for six cycles or generations under stringent growth conditions; and (k) obtaining the microalgae strains. Preferred Method: The steps (B)-(F) are to be performed in a sequential manner to obtain the strains of microalgae with high biomass and lipid content. Preferred Components: The strains have: the ability to grow under salinity conditions of up to 3% (preferably 0.5-3%); heavy metal tolerance of 100-1000 ppm; saturates of 26-85% (preferably 32-70%); lipid content of 20-35% (preferably 26-33%); and biomass of 2-7 g/l (preferably 2-6 g/l). The strains grow in the temperature of 10-45 degrees C. The strains grow in a pH of 4.5-10 (preferably 6.5-10).    TECHNOLOGY FOCUS - ORGANIC CHEMISTRY - Preferred Components: The mutagenizing agents in step (e) are ethyl methanesulfonate (EMS), mitomycin C, N-methyl-N'-nitro-N- nitrosoguanidine, benzo(9)pyrene and 4-nitroquinoline-1-oxide, UV rays, and/or gamma -rays, preferably EMS. The osmotic agent in step (i) is sucrose. EXAMPLE - Ethyl methanesulfonate (EMS) was added into 5 ml of the log phase culture of Chlorella vulgaris IOC-106 in a 15 ml centrifuge tube to a final concentration of 0.42 g/l and the culture suspension was further incubated in a water bath at 45 degrees C for 15 minutes. Diluting the culture 20 times with pre-chilled, fresh subsequently terminated the treatment. The mutated cells were centrifuged and transferred to the media having salt concentration of 1%, heavy metal concentration of 200 ppm, and pH of 10. After 24 hours incubations at 45 degrees C under light conditions and in presence of carbon dioxide, the culture broth was centrifuged at 3000 rpm for 10 minutes and cells which were settled by centrifugation were plated on media agar plates having salt concentration of 1%, high heavy metal concentration of 200 ppm, and high pH of 10. The plates were incubated at 45 degrees C for 48 hours in light and presence of carbon dioxide. For shuffling, protoplasts were fused by suspension in buffer (0.5 M of sucrose, 10 mM of Tris-hydrochloride, and 20 mM of magnesium chloride) containing 15% dimethyl sulfoxide and 60% polyethylene glycol (PEG)-6000. The resulting suspension was incubated at 25 degrees C for 50 minutes. The fused protoplast preparation was diluted with regeneration media (algae culture medium containing 0.5 M of sucrose) and protoplasts were harvested by centrifugation at 3500 rpm for 10 minutes at 25 degrees C. The protoplast cells floating was collected and were re-suspended in regeneration media and shaken at 200 rpm for 12 hours before plating on agar plates having higher salt concentration of 2%, heavy metal concentration of 250 ppm, and pH of 11. The plated were scraped to generate a pooled fusion library. The formation of protoplasts, their fusion and their subsequent regeneration was repeated six times with pooled regenerated cells from one fusion being the inoculum for the subsequent protoplast culture. At each cycle cells were screened at stringent growth conditions with respect to pH, salinity, and heavy metal concentration. Non-shuffled controls were prepared by the recursive formation and regeneration of protoplasts without exposure to PEG. The process was carried out for six cycles to obtain strains of microalgae.