NOVELTY - Twig cuttage cultivation of Nerium oleander, by preparing nursery seedbed, adding substrate to seedbed, selecting scion, cutting leaves, sterilizing cuttings, drying sterilized cuttings, wetting dried cuttings with carbendazim, soaking the cuttings in potassium permanganate, adding substrate to nursery pots, planting cuttings in pots, compacting planted cuttings by filling substrate, watering planted cuttings, spraying chlorothalonil, moistening seedbeds, transplanting seedlings into soil, fertilizing soil, plowing the soil and pruning plants.    USE - The method is useful for twig cuttage cultivation of N.oleander (claimed).    DETAILED DESCRIPTION - Method for twig cuttage cultivation of Nerium oleander, involves (a) preparing nursery seedbed under shed and adding cuttage substrate to the seedbed, (b) choosing cutting period during month of May to July, selecting scion having with normal development, robust growth and no pest and insect infestation, taking branches of 8 months or more and seeds having diameter of 3 mm or more, cutting the leaves on the branches and spike strips measuring 15-20 cm, retaining 2 or more buds on each of the spike strips and cutting plane and smooth U-shaped incision for increasing contact area between the incision with hormones and substrate, promoting wound healing in the spike strips and improving rooting rate of obtained cuttings, (c) sterilizing the cuttings, slotting machines and substrate and disinfecting scissors using 95% ethanol, drying sterilized cuttings, slotting machines and substrate under sunlight for 2 days, wetting dried cuttings, prepared slotting and substrate using 1/3000 carbendazim for 2 hours, soaking the cuttings in 0.06% potassium permanganate solution for 12 hours, further soaking the cuttings in 1/3000 carbendazim solution for 30 minutes, (d) adding the substrate to nursery pots, making an incision up to a depth of 2/3 to 3/4 on left top portion of the cuttings, planting 6 cuttings in each pot at a spacing of 3-5 cm, plugging holes with a bamboo stick, ensuring that the cutting is not let vacant, compacting the planted cuttings by filling the substrate with hands, watering the cuttings, maintaining the planted cuttings under greenhouse shed providing shading up to 85% to the cuttings, maintaining the temperature below 15 degrees C, moisture on surface of the cuttings and relative humidity of 85% under the shed for stabilizing the conditions for plantation of rooting cuttings, gradually lifting shading nets and preventing exposure of the cuttings to pathogens by spraying chlorothalonil diluted up to 1000 times every 5 days on the cuttings, (e) spraying intermittent mist on the cuttings for several times by mist machine before start of callus formation, reducing frequency of mist spraying after formation of the callus on the cuttings, moistening the seedbeds and maintaining temperature of the seedbeds at 18-26 degrees C until sprouting after 2-3 months, and (f) maintaining roots and leaves of grown seedlings intact, transplanting the seedlings at a spacing of 50 cm plus minus 50 cm into soil, where the soil is fertilized by applying farmyard manure as base fertilizer at a rate of 6-7 kg/m2 on the soil, plowing the soil deeply and leveling plowed soil, pruning plants every 1-2 years, cutting top portion of the plants when height of the trunk reaches 30-40 cm for promoting lateral bud germination, selecting 4-5 stout branches on upper portion of the branches, allowing primary branches to grow from the lateral buds, retaining 5-6 lateral buds on the primary branches, cutting upper terminal buds, allowing secondary branches to grow from the buds, retaining 6-7 lateral buds on the secondary branches and allowing the secondary branches to grow, plucking flowers and removing young shoots with poor growth. The substrate is prepared by mixing 90-92 wt.% by-products produced during production of edible vinegar as main material and 8-10 wt.% plant ash as auxiliary material, adding aerobic bacteria to obtained mixture, subjecting the mixture to aerobic fermentation, adding 6-8 wt.% vermiculite, 10-15 wt.% perlite and 15-20 wt.% mushroom compost material, and subjecting obtained to secondary fermentation to obtain fermented product having organic matter content of not less than 50%, soluble ion concentration (electrical conductivity) of 1-3 mS/cm, pH of 5.5-6.5, air voids of 25 vol.% and water holding capacity up to 50 vol.% as substrate. The step (e) involves spraying intermittent mist on the cuttings before start of callus formation preferably during 7-11 am in the morning and 2-6 in the evening for every 1 hour, where during 11 am to 2 pm spraying is performed every 30 minutes for 1-2 minutes, and performing spraying preferably during 7-11 am in the morning and 2-6 in the evening for every 2 hours after formation of callus, where during 11 am to 2 pm spraying is performed every 1 hour for 1-2 minutes.