NOVELTY - Producing biodiesel comprises: (a) converting biologically produced triglycerides to free fatty acids and glycerol; (b) esterifying the free acids to biodiesel; (c) separating the glycerol from the free acids or biodiesel; (d) converting separated glycerol to free fatty acids in a reactor with a recombinant microbe that has been engineered to comprise an overexpressed fatty acyl-acyl carrier protein (ACP) thioesterase (TE) and that converts glycerol to free fatty acids; (e) separating the free fatty acids from the microbe; and (f) converting the separated free fatty acids to biodiesel.    USE - The method is useful for producing biodiesel.    ADVANTAGE - The method makes use of crude glycerol waste product to produce free fatty acids, which can be used to produce single product that is biodiesel which is highly desirable at higher yield without producing added glycerol as a waste product, and thus it is cost effective.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for:    (1) a microbe comprising TE+-NADK+ (where NADK is NAD kinase), TE+-UdhA+ (where UdhA is soluble transhydrogenase), TE+-PntAB+ (where PntAB is membrane-bound transhydrogenase), TE+ NADK+ UdhA+, TE+ NADK+ PntAB+, TE+ FabZ+ NADK+ (where FabZ is beta -hythoxyacyl-ACP), TE+ FabZ+ UdhA+, TE+ FabZ+ PntAB+, TE+ FabZ+ NADK+ UdhA+, TE+ FabZ+ NADK+ PntAB+, Delta fadD+ TE+ NADK+ (FadD is acyl-coenzyme A synthetase), Delta fadD+ TE+ UdhA+, Delta fadD+ TE+ PntAB+, Delta fadD+ TE+ NADK+ UdhA+, Delta fadD+ TE+ NADK+ PntAB+, Delta fadD+ TE+ FabZ+ NADK+, Delta fadD+ TE+ FabZ+ UdhA+, Delta fadD+ TE+ FabZ+ PntAB+, Delta fadD+ TE+ FabZ+ NADK+ UdhA+, Delta fadD+ TE+ FabZ+ NADK+ PntAB+, Delta fadD+ AfabR+ TE+ FabZ+, AfadD+ AfabR+ TE+ FabZ+ NADK+, Delta fadD+ Delta fabR+ TE+ FabZ+ UdhA+, Delta fadD+ Delta fabR+ TE+ FabZ+ PntAB+, Delta fadD+ Delta fabR+ TE+ FabZ+ NADK+ UdhA+, Delta fadD+ Delta fabR+ TE+ FabZ+ NADK+ PntAB+, Delta fadD+ Delta fabR+ TE+ FabZ+ NADK+ glpDFK (where glpDFK is the glycerol transporter (glpF), ATP-dependent glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase (glpD)), TE+ glpDFK+, TE+ ACC+ (where ACC is acetyl-coenzyme A carboxylase), TE+ accABCD+ (where AccABCD is the operon for the 4 ACC subunits), Delta fadD+ Delta fadE+ Delta fadAB+ TE+ (where fadE is the gene encoding acyl-coenzyme A dehydrogenase and fadAB is the gene encoding a multifunctional enzyme complex containing thiolase, 3-hydroxyacyl-coenzyme A dehydrogenase, crotonase, epimerase, and isomerase activities) and glpDFK+ added to any genotype;    (2) producing biodiesel comprising converting a fat or oil to biodiesel and glycerol, separating the biodiesel from the glycerol, converting the separated glycerol to fatty acids in a reactor containing growth medium and a bacteria comprising an overexpressed TE gene, separating the fatty acids from the bacteria and/or the growth medium and converting the separated fatty acids to biodiesel; and    (3) producing biodiesel comprising growing a recombinant bacteria comprising an overexpressed plant acyl-ACP thioesterase in a reactor containing a growth medium comprising a glycerol produced from the synthesis of biodiesel, where the bacteria produces free fatty acids from the glycerol, separating the free fatty acids from the bacteria and/or the growth medium and esterifying the free fatty acids to make additional biodiesel.