NOVELTY - Isolated microorganism that expresses enzymes of the reductive glycine pathway is new.    USE - The isolated microorganism is useful for: converting formate to pyruvate or glycerate via the formation of glycine and serine, and formate to a metabolite of central metabolism via the formation of glycine and without the formation of serine, where the metabolite is acetyl coenzymeA (CoA), oxaloacetate, glycerate 2-phosphate or glycerate 3-phosphate; producing a biofuel including ethanol, propanol, isobutanol, n-butanol, an alcohol, an alkene, an alkane, a lipid or a polysaccharide, where the microorganism is formatotrophic, auto trophic, aerobic, anaerobic, bacteria preferably a gram positive bacteria or a gram negative bacteria, yeast such as Saccharomyces cerevisiae, fungi such as Aspergillus, Candida, Chlamydomonas, Chrysosporium, Cryotococcus, Fusarium, Kluyveromyces, Neotyphodium, Neurospora, Penicillium, Pichia, Saccharomyces, Trichoderma or Xanthophyllomyces, or algae (all claimed).    ADVANTAGE - The microorganism: efficiently converts formate to pyruvate or glycerate with reduced toxicity and with less energy consumption; and produces biofuel with high yield, thermodynamic favorability, chemical motive force, kinetics and expression challenges.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for:    (1) preparation of the microorganism;    (2) a system for culturing the microorganism and an electrode for providing electrons to generate formate;    (3) method-I of selecting the microorganism, comprising growing a microorganism on formate, and analyzing for the production of metabolites of the reductive glycine pathway and/or activity of the enzymes of the reductive glycine pathway in the microorganism, where the production of metabolites and an activity of the enzymes having a predetermined level is indicative of a formatotrophic microorganism;    (4) method-II of generating a biofuel, comprising culturing the microorganism under conditions that allow for biofuel formation, and generating the biofuel; and    (5) method-III of generating a human polypeptide comprising culturing the microorganism under conditions that allow for expression of the human polypeptide, and generating the human polypeptide.