TECHNOLOGY FOCUS - BIOLOGY - Preferred Method: The step (a)-(iii) involves inoculating 4 wt.% seed liquid in fermentation medium comprising 200 pts. wt. potatoes, 8 pts. wt. dextrose, 1 pt. wt. methanol and 1000 pts. wt. water, and culturing at 30 degrees C for 40 hours to obtain fermented broth. The step (a)-(iv) involves mixing 2 pts. wt. fermented broth, 50 pts. wt. water and 20 pts. wt. corn germ, stirring the mixture uniformly, maintaining at 50 degrees C for 40 minutes, stirring the mixture for 1 minute for every 15-20 minutes, and filtering the mixture to obtain phosphorylated enzyme solution. The step (b) involves boiling 30 pts. wt. corn germ and 50 pts. wt. water for 15 minutes, adjusting pH of the extract to 7, adding 0.04 pts. wt. neutral protease to the mixture, stirring the mixture for 4 minutes, maintaining at 50 degrees C for 2.5 hours, stirring the mixture for 4 minutes for every 0.5 hour, and filtering to obtain proteolytic solution. The step (c) involves taking 30 pts. wt. plant ash and 75 pts. wt. water, stirring the mixture, placing the mixture at room temperature for 20-30 minutes, and filtering the mixture to obtain ash extract. In step (d), 15 pts. wt. phosphorylated enzyme solution obtained in step (a), 35 pts. wt. proteolytic solution obtained in step (b) and 50 pts. wt. ash extract are mixed. The P.pastoris strain is P.pastoris strain ACCC21071, P.pastoris strain ACCC21072, P.pastoris strain ACCC21073, P.pastoris strain ACCC21074 or P.pastoris strain ACCC21075. The neutral protease is NE PW30 having enzyme activity of 30000 U/g, NE PW50 having enzyme activity of 50000 U/g or their combination. The neutral protease comprises SUKAPro NEPW50 and SUKAPro NEPW30 in mass ratio of 1:1. The plant ash is crop straw after burning or decomposed organic matter. The crop straw is chosen from corn straw, wheat straw, soybean straw, rice straw, peanut straw, vegetable straw or their combinations. The corn germ is prepared by crushing the corn germ and passing the crushed product through 60-80 mesh sieve. ACTIVITY - Fertilizer.