NOVELTY - Preparation of soilless strawberry seedling breeding culture substrate comprises collecting organic waste alcohol residue, crop straw and coconut husk, drying, crushing, performing water treatment, collecting carbonized rice hull or coal slag powder, vermiculite and perlite; fermenting, inoculating with high temperature fast rotting agent, composting for 2-3 days, turning, adding water to control water content, drying, crushing and sieving; adding coconut shell powder auxiliary materials, and compound fertilizer, and stirring.    USE - Method for preparing soilless strawberry seedling breeding culture substrate (claimed) used for improving strawberry plant disease resistance ability.    ADVANTAGE - The substrate has a grain diameter of 0.5-5 mm, water content of 35-55%, total porosity of 65-75%, bulk density of 0.35-0.5 g/ml, organic matter content /-35%, total nutrient content of 3-5%, electric conductivity (EC) value of 1-2 mS/cm, gas-water ratio of 1:2-4, pH of 5.5-7, brown or black brown color and no peculiar smell.    DETAILED DESCRIPTION - Preparation of soilless strawberry seedling breeding culture substrate comprises collecting organic waste alcohol residue, crop straw and coconut husk, drying, crushing, performing water treatment, collecting carbonized rice hull or coal slag powder, vermiculite and perlite as auxiliary material; where amount of raw materials are 3-5 pts. vol. mixed fermented material of alcohol residue and straw powder, 1-3 pts. vol. coconut shell powder base material, 1-2 pts. vol. carbonized rice hull powder or coal slag powder, 0.5-1 pts. vol. perlite, 1-2 pts. vol. vermiculite, and 0.1-0.5 wt.% compound fertilizer; putting organic material in fermentation groove, carrying out composting fermentation at a depth of 1-1.5 m, or stacking on cement fermenting field at a width of 3 m, height of 0.8-1.2 m, and length of 5-50 m, inoculating with high temperature fast rotting agent, composting for 2-3 days, adjusting frequency of turning to 1-3 times a day when temperature rises to more than 55 degrees C after one week, adding water to control water content to 55-65%, drying, crushing and sieving; adding coconut shell powder auxiliary materials, and compound fertilizer, and stirring.