NOVELTY - An artificial antigen and antibody (I) and (II), is new. A hapten is connected with bovine serum albumin of artificial antigens, synthetic enzyme horseradish peroxidase to immunize New Zealand rabbits, isolated and purified antisera prepared antibodies.    USE - The artificial antigen and antibody are useful for providing herbicidal effect (claimed).    ADVANTAGE - The artificial antigen and antibody have excellent stability, specificity and sensitivity. The method is simple and environmentally-friendly.    DETAILED DESCRIPTION - An artificial antigen and antibody of formulae (I) and (II), is new. A hapten is connected with bovine serum albumin of artificial antigens, synthetic enzyme horseradish peroxidase to immunize New Zealand rabbits, isolated and purified antisera prepared antibodies. An INDEPENDENT CLAIM is included for method for preparing the artificial antigen and antibody, which involves (a) adding 27.5 ml distilled water, 4 ml N-methyl pyrrolidone and 5.7 g barium hydroxide to 100 ml flask, heating and refluxing mixture for 3 hours, filtering to obtain crude product N-methyl butyric acid, adding 46.8 ml distilled water, 5.416 mL N-methyl caprolactam and 2.884 ml concentrated sulfuric acid, stirring mixture, heating and refluxing for 10 hours, regulating pH to 10, filtering, washing, and evaporating filtrate, (b) dissolving 2 mmol N-methyl butyric acid and 2 mmol 3-(trifluoromethyl)phenyl isocyanate in 4.2 ml sodium hydroxide solution, stirring mixture, reacting mixture for 2 hours, filtering, removing precipitate, removing upper solution, filtering, cooling and crystallizing mixture, (c) weighing 4.307 mg hapten in brown bottle, adding 200 g dimethyl sulfoxide, adding 8.711 mg N-hydroxy succinic imide, 6.242 mg N,N-carbodiimide, and stirring mixture for 24 hours, and (d) artificially synthesizing active ester liquid, dropping 20 mg bovine serum albumin (BSA), maintaining mixture at 4 degrees C overnight, measuring the concentration, packaging, and storing at 20 degrees C.