NOVELTY - Preparing microbial fertilizer composition, by activating cryopreserved photosynthetic bacteria, nitrogen-fixing bacteria, silicate bacteria, Cyanobacteria and yeast, inoculating activated strains on respective sterilized liquid culture media, inoculating and culturing culture broths on sterilized fermentation culture medium, inoculating and culturing liquid culture on primary culture medium, inoculating and culturing primary seed culture on sterilized fermentation culture medium, and inoculating secondary seed culture on sterilized fermentation culture medium.    USE - The method is useful for preparing microbial fertilizer composition for planted nursery seedlings (claimed), preferably for promoting growth of the nursery seedlings.    ADVANTAGE - The method enables to prepare microbial fertilizer composition that increases survival rate of the planted nursery seedlings, and is environmentally-friendly and cost-effective.    DETAILED DESCRIPTION - Method for preparing microbial fertilizer composition, involves (a) activating microorganisms chosen from photosynthetic bacteria, nitrogen-fixing bacteria, silicate bacteria, Cyanobacteria and yeast that are cryopreserved on culture slants and placing activated microbial strains in Petri dish, (b) inoculating activated photosynthetic bacterial strains on sterilized liquid culture medium (CM1), culturing the photosynthetic bacterial strains at 28-30 degrees C under an anaerobic condition and light illumination of tungsten filament lamp for 5-7 days to obtain culture broth (CB1), inoculating activated nitrogen-fixing bacterial strains on sterilized liquid culture medium (CM2), culturing the nitrogen-fixing bacterial strains at 25-27 degrees C under an aerobic condition for 1-2 days to obtain culture broth (CB2), inoculating activated silicate bacterial strains on sterilized liquid culture medium (CM3), culturing the silicate bacterial strains at 28-30 degrees C under an aerobic condition for 1-2 days to obtain culture broth (CB3), inoculating activated Cyanobacteria on sterilized liquid culture medium (CM4), culturing the Cyanobacteria at 28-30 degrees C under light illumination of fluorescent lamp (10-14 hours/day) for 3-5 days to obtain culture broth (CB4), inoculating activated yeast strains on sterilized liquid culture medium (CM5), and culturing the yeast strains at 28-30 degrees C under an aerobic condition for 3 days to obtain culture broth (CB5), (c) inoculating activated culture broths (CB1)-(CB5) in a respective ratio of 2:2:2:2:1 on sterilized fermentation culture medium (M1), performing culturing process at 28-30 degrees C under an aerobic condition for 3 days to obtain liquid cultures, inoculating obtained liquid culture on primary culture medium, and culturing inoculated strains at 37 degrees C under an anaerobic condition for 3 days to obtain primary seed culture, (d) inoculating 5% primary seed culture on sterilized fermentation culture medium (M2), culturing the primary seed culture at 28-30 degrees C under an aerobic condition for first 3-5 days and further under an anaerobic condition for next 3-5 days to obtain secondary seed culture, and (e) inoculating 5% secondary seed culture on sterilized fermentation culture medium (M3), culturing the secondary seed culture at 28-30 degrees C under an aerobic condition for first 3-5 days and under an anaerobic condition for next 3-5 days. The liquid culture medium (CM1) comprises 10 g yeast extract, 1 g dipotassium hydrogen phosphate, 0.5 g magnesium sulfate and 1000 ml water, and has pH of 7-7.2. The liquid culture medium (CM2) comprises 0.5 g yeast extract, 20 g mannitol, 0.2 g potassium dihydrogen phosphate, 0.8 g dipotassium hydrogen phosphate, 0.2 g magnesium sulfate, 0.1 g calcium sulfate, 0.01 g ferric chloride, 0.01 g sodium chloride and 1000 ml water, and has pH of 7.2. The liquid culture medium (CM3) comprises 10 g sucrose, 5 g calcium sulfate, 0.2 g dipotassium hydrogen phosphate, 5 g calcium carbonate, 0.2 g magnesium sulfate, 0.2 g sodium chloride and 1000 ml water, and has pH of 7.5. The liquid culture medium (CM4) comprises 1.5 g sodium nitrate, 0.05 g dipotassium hydrogen phosphate, 0.08 g magnesium sulfate, 0.04 g calcium chloride, 0.02 g sodium carbonate, 0.006 g citric acid, 0.006 g ferric citrate, 0.001 g zinc sulfate, 0.001 g manganese chloride and 1000 ml water, and has pH in the neutral range. The liquid culture medium (CM5) comprises 200 g potato starch, 20 g sucrose, and 1000 ml water, and has pH in the neutral range. The fermentation culture medium (M1) comprises 5% orange water, 0.5% yeast extract, 1% peptone, 0.2% ammonium chloride, 0.1% sodium chloride, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.025% zinc sulfate, 0.025% ferrous sulfate and water (remainder). The fermentation culture medium (M2) comprises 5% orange water, 0.1% yeast extract, 0.1% peptone, 0.2% ammonium chloride, 0.1% sodium chloride, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.025% zinc sulfate, 0.025% ferrous sulfate and water (remainder). The fermentation culture medium (M3) comprises 5% orange water, 0.2% ammonium chloride, 0.1% sodium chloride, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.025% zinc sulfate, 0.025% ferrous sulfate and water (remainder).