NOVELTY - Extracting spinosad from Saccharopolyspora spinosa fermentation liquid comprises (a) pretreating S. spinosa fermentation liquid with alkali liquid containing aqueous sodium hydroxide solution, regulating pH, stirring, and centrifuging for solid-liquid separation; (b) absorbing organic matter of aqueous phase by adding adsorbent, stirring, centrifuging and collecting solids, and performing secondary centrifugation; (c) combining the two separated solids, and vacuum-drying; (d) subjecting the dried product to Soxhlet extraction; and (e) recrystallizing, and removing solvent.    USE - The method is useful for extracting spinosad from Saccharopolyspora spinosa fermentation liquid (claimed).    ADVANTAGE - The method: does not require addition of a polar solvent for pretreatment process; greatly reduces the organic solvent amount; reduces pollution and cost; has safe operation condition and good separation effect; greatly reduces chemical oxygen demand (COD) in the waste water to reduce wastewater treatment difficulty; has high spinosad product yield of 70-95%; produces purified high spinosad having 75-93.2% quality.    DETAILED DESCRIPTION - Extracting spinosad from Saccharopolyspora spinosa fermentation liquid comprises (a) pretreating S. spinosa fermentation liquid with alkali liquid containing 20-50 wt.% aqueous sodium hydroxide solution, regulating the fermentation liquid pH to 8-10, stirring at room temperature for 15-30 minutes, and then centrifuging for solid-liquid separation; (b) absorbing organic matter of the resulting aqueous phase by adding 0.5-1 wt.% adsorbent at 5-50 degrees C, stirring for 15-60 minutes, centrifuging and collecting solids, performing secondary centrifugation, and then conveying aqueous phase for wastewater treatment, where the adsorbent is bentonite, activated carbon, macroporous resin or chitosan; (c) combining the two separated solids, and then vacuum-drying at 30-80 degrees C for 1-8 hours; (d) then subjecting the dried product to Soxhlet extraction using an extraction agent comprising pentane, methanol, ethanol, n-hexane, cyclohexane, petroleum ether, ethyl acetate, dichloromethane or trichloromethane, and refluxing for 1-8 hours, where the amount of the solvent is 0.5-5 times of the solid mass; and (e) recrystallizing using solvent to obtain spinosad product, and then concentrating under reduced pressure to remove solvent, where the solvent is n-pentane, n-hexane, cyclohexane or petroleum ether.