NOVELTY - Preparing taxol from yew cell culture liquid, comprises performing solid-liquid separation to cell culture solution, filtering the cell wall, then mixing the stock solution and the filtrate, purifying the mixed liquid, washing, eluting adding extracting agent into eluent, liquid-liquid extracting, drying organic solvent layer, adding basic alumina into the crude product, eluting, drying eluting part in vacuum, purifying the sample, eluting, collecting alcohol elution part, volatilizing organic phase to obtain crystal, centrifuging to collect crystal part, and freeze-drying collected crystal.    USE - The method is useful for preparing taxol from yew cell culture liquid (claimed).    ADVANTAGE - The method is capable of preparing taxol with high recovery rate and high purity in a simple, convenient and eco-friendly manner.    DETAILED DESCRIPTION - Preparing taxol from yew cell culture liquid, comprises performing solid-liquid separation to cell culture solution to obtain stock solution and cell wall, and then back filtering the cell wall to obtain filtrate, then mixing the stock solution and the filtrate, purifying the mixed liquid by non-polar macro-porous absorption resin chromatographic column, washing using distilled water, eluting using ethanol solution, collecting the eluent, recovering the ethanol, adding extracting agent into eluent, performing liquid-liquid extraction for two times, collecting organic solvent layer, vacuum drying at low temperature to obtain macro-porous resin column enrichment taxol crude product, adding the crude product with chromatographic column with basic alumina as filler, eluting with elution solution, collecting eluting part, drying in vacuum to obtain sample, purifying the sample by 18C high efficient liquid phase chromatographic column, then eluting with flowing phase methanol solution, collecting alcohol elution part, drying the component in a ventilation place, volatilizing organic phase at 4 degrees C in refrigerator for 24 hours to obtain crystal, centrifuging and collecting the crystal part, then continuously centrifuging and collecting the supernatant, and freeze-drying collected crystal.