NOVELTY - Method for producing high quality microalgae biodiesel by preparing seed culture medium and inoculating Chlorella vulgaris in seed culture medium, performing culturing process, collecting the prepared seed and performing one stage fermentation process, centrifuging the fermented seed to obtain algae mud, performing two stage fermentation process on the algae mud, recovering the fermented product by centrifugation, chemical flocculation or floating, and extracting and crushing the recovered product.    USE - The method is useful for producing high quality microalgae biodiesel (claimed).    DETAILED DESCRIPTION - Method for producing high quality microalgae biodiesel by preparing seed culture medium and inoculating Chlorella in seed culture flask, adding the inoculated flask to photo-biological reaction device, performing illumination culture in good extinguishing bacteria culture medium comprising carbon dioxide storage bottle, mixing device and gas flow meter, where seed culture medium comprises 24-34 g/l salt, 0.3-0.5 g/l sodium acetate salt, 200-300 mg/l sodium nitrate, 50-100 mg/l sodium dihydrogen phosphate, 10 mg/l disodium EDTA, 2.5-5 mg/l iron(II) sulfate heptahydrate, 0.006-0,06 mg/l vitamin B1, and 0.00005-0.0005 mg/l vitamin B2, the light source is fluorescent lamp or LED, the illumination intensity is 8-l0 Klux, the intensity is 0.2-0.7 VVM, the concentration of carbon dioxide is 0-0.5%, the culturing process is performed at 23-26 degrees C for 3-5 days, collecting the seed prepared in one-stage fermentation, centrifuging the collected seed at 4000-12000 revolutions/minute for l0 minutes to obtain algae mud, washing the algae mud using clean water for 2-3 times, centrifuging the obtained algae mud, and resuspending the precipitate, to obtain the subsequent fermentation seed, adding the first stage fermented seed into photo-reactor filled with fermentation culture medium with the above-mentioned raw materials and performing culturing process using the above-mentioned culture conditions, performing second stage fermentation process, recovering the fermented product by centrifugation, chemical flocculation or floating, extracting and crushing the recovered product by enzymatic crushing, ultrasonic crushing or liquid nitrogen, and the extraction is performed by using oil and organic solvent including chloroform or methanol and water in a ratio of 1: 1 or ether or normal hexane and water in a ratio of 1:1 at normal temperature.