TECHNOLOGY FOCUS - BIOLOGY - Preferred Components: The host cell is a fungal cell or a bacterial cell. The host cell is selected from: a filamentous fungal cell selected from: Trichoderma reesei, Trichoderma longibrachiatum, Trichoderma viride, Trichoderma koningii, Trichoderma harzianum, Penicillium, Humicola, Humicola insolens, Humicola grisea, Chrysosporium, Chrysosporium lucknowense, Myceliophthora thermophilia, Gliocladium, Aspergillus, Fusarium, Neurospora, Hypocrea, Emericella, Aspergillus niger, Aspergillus awamori, Aspergillus aculeatus, and Aspergillus nidulans; a yeast cell selected from: Saccharomyces cervisiae, Schizzosaccharomyces pombe, Schwanniomyces occidentalis, Kluveromyces lactus, Candida utilis, Candida albicans, Pichia stipitis, Pichia pastoris, Yarrowia lipolytica, Hansenula polymorpha, Phaffia rhodozyma, Arxula adeninivorans, Debaryomyces hansenii, and Debaryomyces polymorphus; and Zymomonas mobilis bacterial cell. The host cell expresses the variant of a parent CBH polypeptide encoded by the isolated polynucleotide, vector, or expression vector. The cell culture supernatant is derived from a culture of the host cell. The cell culture supernatant further comprises additional cellulases or hemicellulases. The additional cellulases and/or hemicellulases are: exogenously expressed in the host cell, endogenously expressed in the host cell, and/or mixed with the cell culture supernatant.    TECHNOLOGY FOCUS - BIOTECHNOLOGY - Recombinant Method (claimed): Production of variant CBH polypeptide involves culturing the host cell in a suitable culture medium under suitable conditions to produce the variant; and further isolating the produced variant. Preferred Variant: The variant comprises an amino acid substitution selected from: Thr356Leu, Thr246Pro, Thr255Asp and/or Asn200Arg, where the position of each amino acid substitution corresponds to (SEQ ID NO: 3). The variant further comprises an amino acid substitution selected from: Thr246Ser and Thr246Val; or an amino acid substitution selected from Thr255Ile, Thr255Lys, Thr255Pro, Thr255Arg and Thr255Val; or Asn200Gly amino acid substitution; or an amino acid substitution selected from: Phe418Met, Asp241Asn, Glu234Asp, Pro194Val, Tyr247Asp, Asn49Pro, Ser92Thr and/or Thr41Ile. Preferred Components: The parent CBH polypeptide is a fungal cellobiohydrolase 1 (CBH1) selected from Hypocrea jecorina, Hypocrea schweinitzii, Hypocrea orientalis, Trichoderma pseudokoningii, Trichoderma konilangbra, Trichoderma citrinoviride, Trichoderma harzanium, Aspergillus aculeatus, Aspergillus niger, Penicillium janthinellum, Humicola grisea, Scytalidium thermophilum, or Podospora anderina. The parent CBH polypeptide has at least 90% sequence identity to (SEQ ID NO: 3). Preferred Method: The method (p1) further involves killing the host cells after the culturing step; filtering the collected cell culture supernatant to remove cell debris; and subjecting the cell culture supernatant to ultrafiltration or other steps to enrich or concentrate the CBH variant, where the host cell further expresses additional cellulases and/or hemicellulases, where the additional cellulases and/or hemicellulases are present in the cell culture supernatant. The method (p1) further involves contacting the cell culture supernatant with a lignocellulosic biomass substrate. Preferred Vector: The vector is an expression vector. EXAMPLE - No suitable example given.