NOVELTY - Separating and purifying chenodeoxycholic acid from goose bile involves saponifying (in wt.%): goose bile (6-8) and solid sodium hydroxide on a water bath at 95 degrees C for 15-20 hours. Solid calcium chloride (8-10%) is added to the obtained mixture and then centrifuged to obtain precipitate of calcium salt of cholic acid. The obtained precipitate is processed thricely with water in agitated condition for 50-60 minutes and then centrifuged to collect the supernatant, where pH of the supernatant is maintained at 3 by using hydrochloric acid.    USE - Method for separating and purifying chenodeoxycholic acid from goose bile (claimed).    ADVANTAGE - The chenodeoxycholic acid separating and purifying method enables to secure repetition of processing steps without using ethyl acetate in order to reduce environment pollution. The method ensures high yielding rate of the product by using simple process.    DETAILED DESCRIPTION - Separating and purifying chenodeoxycholic acid from goose bile involves saponifying (in wt.%): goose bile (6-8) and solid sodium hydroxide on a water bath at 95 degrees C for 15-20 hours. Solid calcium chloride (8-10%) is added to the obtained mixture and then centrifuged to obtain precipitate of calcium salt of cholic acid. The obtained precipitate is processed thricely with water in agitated condition for 50-60 minutes and then centrifuged to collect the supernatant, where pH of the supernatant is maintained at 3 by using hydrochloric acid. The obtained precipitate is centrifuged to collect chenodeoxycholic acid in crude form. The crude product is mixed with sodium hydroxide in 1:1 ratio and then heated to homogenize the mixture. The mixture is diluted with water (25 times of the mixture). The obtained liquid is processed through macroporous resin chromatographic column, where ethanolic solution (4-60%) is utilized as eluent to elute the liquid and obtain colorless and transparent liquid as a product. The obtained product is eluted with 5% of sodium hydroxide solution (4 times of the obtained product), where pH of the obtained product is maintained at 3 by using hydrochloric acid. The final product is centrifuged to obtain chenodeoxycholic acid.