TECHNOLOGY FOCUS - BIOTECHNOLOGY - Preferred Components: The coconut water is obtained from coconut fruit by pooling from multiple coconuts or from single coconut individually. The nitrogen source is chosen from ammonium sulfate, ammonium acetate, ammonium carbonate, ammonium chloride and amino acid mixture or their combinations. The nitrogen source is 10-100 mM, preferably 25 mM ammonium sulfate. The 1-10 mM, preferably 2 mM magnesium salt is chosen from magnesium chloride, magnesium carbonate and magnesium sulfate. The excipient is chosen from inducer, activator, antibiotic and their combinations, at appropriate concentration. The pH of the culture medium is 4-8. The prokaryotic cell is Escherichia coli and the eukaryotic cell is Pichia pastoris. Preferred Method: In method (M1), the coconut water is extracted from a single coconut fruit or multiple coconut fruits, and upon the extraction, solid particles are optionally removed from the coconut water followed by aseptic sterilization or by filter sterilizing the water before combining with the nitrogen source. The coconut water extracted from multiple coconut fruits is pooled before optional removal of solid particles and filter sterilization. The removal of the solid particles from the coconut water is carried out by centrifuging at 3000-10000 rotations per minute for 15-30 minutes, subjecting to filter sterilization using membrane filter having 0.01-0.3 mu m, preferably 0.2 mu m pore size. In method (M2), the culture medium enhances the growth or biomass concentration of E.coli and P.pastoris by 2-10 folds and 1.5-3 folds, respectively comparing with growth or biomass concentration when grown in existing media. The prokaryotic or eukaryotic cell is grown in the culture medium by a method chosen from batch-cultivation, perfusion cultivation and chemostat cultivation. EXAMPLE - Coconut was collected and part of its mesocarp and endocarp were removed to expose the surface of endosperm. Then, coconut water (CW) was collected in sterile container and centrifuged at 4000 rotations per minute for 20 minutes to remove any solid particles, followed by filter sterilization with 0.22 mu m filter. 300-500 ml CW was obtained from one coconut fruit, and pH of the CW was noted as 4.6-4.8. Nitrogen source in the form of ammonium based salts such as ammonium sulfate, ammonium acetate, ammonium carbonate and ammonium chloride was added to CW, and were mixed together to obtain CW culture medium.