NOVELTY - High speed countercurrent chromatographic separation and purification of harpagoside includes crushing figwort medicinal material into coarse powder, adding ethanol as solvent, extracting by heating at reflux, filtering, cooling the filtrate, removing the solvent, adding ethanol extract into macroporous absorption resin, gradient eluting, collecting eluate containing harpagoside, removing solvent, drying, obtaining crude harpagoside, subjecting to high speed countercurrent chromatography using e.g. methanol, ethyl acetate, and water, UV detecting, collecting eluate, concentrating and drying.    USE - High speed countercurrent chromatographic separation and purification of harpagoside (claimed).    ADVANTAGE - The method avoids sample loss, achieves good separation effect and less solvent consumption and is pollution-free, efficient and fast.    DETAILED DESCRIPTION - High speed countercurrent chromatographic separation and purification of harpagoside comprises crushing figwort medicinal material into coarse powder, adding 10-95 vol.% ethanol as solvent, extracting for 1-3 times by heating at reflux, obtaining extracting liquid, filtering, cooling the filtrate, decompressing to remove the solvent, obtaining ethanol extract, adding ethanol extract into macroporous absorption resin, gradient eluting with mixed solvent of water and methanol or ethanol, collecting eluate containing harpagoside, decompressing to remove the solvent, drying, obtaining crude harpagoside, subjecting to high speed countercurrent chromatography using solvent A, solvent B and solvent C at 1-5:5-10:1-10 volume ratio at speed of 500-1000 revolutions/minute, where solvent A is methanol, ethanol, n-butyl alcohol or isobutyl alcohol, solvent B is ethyl acetate, propyl acetate or n-butyl acetate and solvent C is water, taking upper phase as stationary phase and lower phase as mobile phase, where flow rate of mobile phase is 0.5-5 ml/minute, UV detecting at wavelength of 190-380 nm, collecting eluate, concentrating and drying to obtain harpagoside.